RESUMO
Anophthalmia, microphthalmia, and coloboma are a genetically heterogeneous spectrum of developmental eye disorders and affect around 30 per 100,000 live births. OLFM2 encodes a secreted glycoprotein belonging to the noelin family of olfactomedin domain-containing proteins that modulate the timing of neuronal differentiation during development. OLFM2 SNPs have been associated with open angle glaucoma in a case-control study, and knockdown of Olfm2 in zebrafish results in reduced eye size. From a cohort of 258 individuals with developmental eye anomalies, we identified two with heterozygous variants in OLFM2: an individual with bilateral microphthalmia carrying a de novo 19p13.2 microdeletion involving OLFM2 and a second individual with unilateral microphthalmia and contralateral coloboma who had a novel single base change in the 5' untranslated region. Dual luciferase assays demonstrated that the latter variant causes a significant decrease in expression of OLFM2. Furthermore, RNA in situ hybridisation experiments using human developmental tissue revealed expression in relevant structures, including the lens vesicle and optic cup. Our study indicates that OLFM2 is likely to be important in mammalian eye development and disease and should be considered as a gene for human ocular anomalies.
Assuntos
Proteínas da Matriz Extracelular/genética , Anormalidades do Olho/genética , Glaucoma de Ângulo Aberto/genética , Glicoproteínas/genética , Polimorfismo de Nucleotídeo Único , Linhagem Celular Tumoral , Estudos de Coortes , Olho/embriologia , Anormalidades do Olho/diagnóstico , Proteínas do Olho/genética , Deleção de Genes , Regulação da Expressão Gênica , Glaucoma de Ângulo Aberto/diagnóstico , Glaucoma de Ângulo Aberto/etiologia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Lactente , MasculinoRESUMO
Interstitial deletions of chromosome 3p14p12 are a rare chromosome rearrangement. Twenty-six patients have been reported in the literature to date, however, a specific clinical phenotype has not yet been delineated. We describe three patients (two new) with overlapping chromosome 3p14p12 deletions and review the clinical and molecular data of 11 well-characterized, published cases. These patients had a number of features in common, such as short stature, failure to thrive, facial dysmorphism, congenital heart defects, urogenital abnormalities, neurological problems, hearing loss, and global developmental delay, suggesting that the interstitial chromosome 3p14p12 deletion gives rise to a multiple congenital anomaly syndrome. Some of the patients show clinical overlap with other complex syndromes such as CHARGE syndrome. Genotype-phenotype analysis revealed candidate genes for parts of the clinical features suggesting that the 3p14 deletion is a contiguous gene syndrome.
Assuntos
Anormalidades Múltiplas/genética , Deleção Cromossômica , Cromossomos Humanos Par 3/química , Deficiências do Desenvolvimento/genética , Anormalidades Múltiplas/patologia , Criança , Pré-Escolar , Mapeamento Cromossômico , Deficiências do Desenvolvimento/patologia , Feminino , Genótipo , Humanos , Lactente , Masculino , Fenótipo , Índice de Gravidade de Doença , SíndromeRESUMO
OBJECTIVES: To estimate procedure-related risks of miscarriage following amniocentesis and chorionic villus sampling (CVS) based on a systematic review of the literature and a meta-analysis. METHODS: A search of MEDLINE, EMBASE, CINHAL and The Cochrane Library (2000-2014) was performed to review relevant citations reporting procedure-related complications of amniocentesis and CVS. Only studies reporting data on more than 1000 procedures were included in this review to minimize the effect of bias from smaller studies. Heterogeneity between studies was estimated using Cochran's Q, the I(2) statistic and Egger bias. Meta-analysis of proportions was used to derive weighted pooled estimates for the risk of miscarriage before 24 weeks' gestation. Incidence-rate difference meta-analysis was used to estimate pooled procedure-related risks. RESULTS: The weighted pooled risks of miscarriage following invasive procedures were estimated from analysis of controlled studies including 324 losses in 42 716 women who underwent amniocentesis and 207 losses in 8899 women who underwent CVS. The risk of miscarriage prior to 24 weeks in women who underwent amniocentesis and CVS was 0.81% (95% CI, 0.58-1.08%) and 2.18% (95% CI, 1.61-2.82%), respectively. The background rates of miscarriage in women from the control group that did not undergo any procedures were 0.67% (95% CI, 0.46-0.91%) for amniocentesis and 1.79% (95% CI, 0.61-3.58%) for CVS. The weighted pooled procedure-related risks of miscarriage for amniocentesis and CVS were 0.11% (95% CI, -0.04 to 0.26%) and 0.22% (95% CI, -0.71 to 1.16%), respectively. CONCLUSION: The procedure-related risks of miscarriage following amniocentesis and CVS are much lower than are currently quoted.
Assuntos
Aborto Espontâneo/etiologia , Amniocentese/efeitos adversos , Amostra da Vilosidade Coriônica/efeitos adversos , Diagnóstico Pré-Natal/efeitos adversos , Aborto Espontâneo/epidemiologia , Aneuploidia , Tomada de Decisões , Feminino , Idade Gestacional , Humanos , Razão de Chances , Educação de Pacientes como Assunto , Gravidez , Fatores de RiscoAssuntos
Terapia Baseada em Transplante de Células e Tecidos/métodos , Fertilização in vitro/métodos , Células Germinativas , Células-Tronco Pluripotentes Induzidas , Infertilidade Feminina/terapia , Infertilidade Masculina/terapia , Animais , Modelos Animais de Doenças , Feminino , Gametogênese/fisiologia , Humanos , Masculino , Casamento , Camundongos , TrissomiaRESUMO
BACKGROUND: Congenital deletions affecting 3q11q23 have rarely been reported and only five cases have been molecularly characterised. Genotype-phenotype correlation has been hampered by the variable sizes and breakpoints of the deletions. In this study, 14 novel patients with deletions in 3q11q23 were investigated and compared with 13 previously reported patients. METHODS: Clinical data were collected from 14 novel patients that had been investigated by high resolution microarray techniques. Molecular investigation and updated clinical information of one cytogenetically previously reported patient were also included. RESULTS: The molecular investigation identified deletions in the region 3q12.3q21.3 with different boundaries and variable sizes. The smallest studied deletion was 580 kb, located in 3q13.31. Genotype-phenotype comparison in 24 patients sharing this shortest region of overlapping deletion revealed several common major characteristics including significant developmental delay, muscular hypotonia, a high arched palate, and recognisable facial features including a short philtrum and protruding lips. Abnormal genitalia were found in the majority of males, several having micropenis. Finally, a postnatal growth pattern above the mean was apparent. The 580 kb deleted region includes five RefSeq genes and two of them are strong candidate genes for the developmental delay: DRD3 and ZBTB20. CONCLUSION: A newly recognised 3q13.31 microdeletion syndrome is delineated which is of diagnostic and prognostic value. Furthermore, two genes are suggested to be responsible for the main phenotype.
Assuntos
Deleção Cromossômica , Cromossomos Humanos Par 3 , Deficiências do Desenvolvimento/genética , Fácies , Genitália Masculina/anormalidades , Transtornos do Crescimento/genética , Deficiências do Desenvolvimento/diagnóstico , Feminino , Estudos de Associação Genética , Humanos , Masculino , Proteínas do Tecido Nervoso/genética , Receptores de Dopamina D3/genética , Síndrome , Fatores de Transcrição/genéticaRESUMO
Since the first report in 1993, an ectopic centromere, i.e. neocentromere formation, has been reported in more than 100 small supernumerary marker chromosomes (sSMC), in 7 instances of centromere repositioning, and in about a dozen cases with more complex chromosomal rearrangements. Here we report 2 new cases with centromere repositioning and 3 neocentric sSMC consisting exclusively of heterochromatic material. Yet, no centromere formation was reported for the regions 18q22.1 and Xq27.1â¼27.2 as it was observed in the 2 cases with centromere repositioning here; in both cases, cytogenetically an inversion was suggested. Two of the 3 neocentric sSMC were derived from a short arm of an acrocentric chromosome. The remainder neocentric sSMC case was previously reported and was stainable only by material derived from itself.
Assuntos
Centrômero , Cromossomos Humanos Par 18 , Cromossomos Humanos X , Adulto , Feminino , Humanos , Hibridização in Situ Fluorescente , Lactente , Masculino , GravidezRESUMO
In this study we model the impact of introducing outpatient investigation of pulmonary thrombo-embolism (PTE) to the acute medical unit (AMU) using the Pulmonary Embolism Severity Index (PESI) decision rule. Specifically, we ask what proportion of patients requiring imaging could be investigated without admission, and how many bed-days this would save. We obtained records for all medical patients who had imaging for PTE in a six-month period at a large teaching hospital with a 40-bedded AMU. The patients were categorized into suitability for outpatient investigation using a combination of the PESI rule and practical considerations. Three hundred and fifty-nine separate presentations were identified. From available records, 31 patients (9.2%, 95% confidence interval [CI] 6.6-12.8%) had no contraindications to outpatient management. These patients used a total of 79 bed-days in the six-month period, or 1.1% (95% CI 0.8-1.5%) of the maximum AMU bed occupancy. Around 10% of patients who require imaging for suspected PTE could be triaged to outpatient investigation using the PESI tool. Adopting this method to triage patients of ambulatory care, would have only a modest effect on acute medical bed occupancy, but remains a valid option for motivated patients in the low-risk category.
Assuntos
Assistência Ambulatorial/métodos , Seleção de Pacientes , Embolia Pulmonar/diagnóstico , Triagem , Idoso , Técnicas de Apoio para a Decisão , Feminino , Hospitalização , Humanos , Masculino , Valor Preditivo dos Testes , Encaminhamento e Consulta , Reprodutibilidade dos Testes , Estudos Retrospectivos , Medição de Risco , Escócia/epidemiologia , Índice de Gravidade de DoençaRESUMO
BACKGROUND: The aim of this study was to ascertain the prevalence of meiotic segregation products in embryos from carriers of 13/14 and 14/21 Robertsonian translocations and to estimate the predictive value of testing single cells using the fluorescence in situ hybridization (FISH) technique, to provide more information for decision-making about PGD. METHODS: In this prospective cohort study, the copy number of translocation chromosomes in nuclei from lysed blastomeres of cleavage-stage embryos was ascertained using locus-specific FISH probes. Logistic regression analysis, controlling for translocation type, female age and fertility status, was used to calculate the odds ratio (OR) of unbalanced segregation products for female and male heterozygotes. The primary diagnostic measure was the predictive value of the test result. The primary outcome measure was the live birth rate per couple. RESULTS: Female carriers were four times more likely than male carriers to produce embryos with an unbalanced translocation product (OR 3.8, 95% confidence interval 2.0-7.2, P < 0.001). The prevalence of abnormality for the chromosomes tested in embryos from female or male heterozygotes was estimated to be 43 or 28%, respectively, while estimates of the predictive value were 93-100 or 96-100% for a normal test result and 79 or 57% for an abnormal test result. The live birth rate per couple was 58% for female carriers and 50% for male carriers. CONCLUSIONS: For female carriers, PGD using FISH could reduce the risk of miscarriage from either translocation or the risk of Down syndrome from the 14/21 Robertsonian translocation. PGD using FISH for male carriers is unlikely to be indicated given the relatively low prevalence of chromosome imbalance and low predictive value.
Assuntos
Segregação de Cromossomos/genética , Fase de Clivagem do Zigoto , Meiose , Diagnóstico Pré-Implantação/métodos , Translocação Genética , Cromossomos Humanos Par 13/genética , Cromossomos Humanos Par 14/genética , Cromossomos Humanos Par 21/genética , Estudos de Coortes , Feminino , Heterozigoto , Humanos , Hibridização in Situ Fluorescente/métodos , Masculino , GravidezRESUMO
Dopamine agonist therapy has been the cornerstone treatment for prolactinoma since the 1970s, replacing surgery in the primary management of this condition. These agents are effective in the management of prolactin excess, have a low side-effect profile, and in some cases may even be curative. However, recent studies of high dose dopamine agonists used in Parkinson's disease have raised the possibility that these drugs may be associated with cardiac valvulopathy. This paper discusses the modern use of dopamine agonists in a patient with prolactinoma.
Assuntos
Comportamento de Escolha , Agonistas de Dopamina/uso terapêutico , Prolactinoma/tratamento farmacológico , Animais , Humanos , Resultado do TratamentoRESUMO
In 2016, 10 universities launched a Networked Improvement Community (NIC) aimed at increasing the number of scholars from Alliances for Graduate Education and the Professoriate (AGEP) populations entering science, technology, engineering, and mathematics (STEM) faculty careers. NICs bring together stakeholders focused on a common goal to accelerate innovation through structured, ongoing intervention development, implementation, and refinement. We theorized a NIC organizational structure would aid understandings of a complex problem in different contexts and accelerate opportunities to develop and improve interventions to address the problem. A distinctive feature of this NIC is its diverse institutional composition of public and private, predominantly white institutions, a historically Black university, a Hispanic-serving institution, and land grant institutions located across eight states and Washington, DC, United States. NIC members hold different positions within their institutions and have access to varied levers of change. Among the many lessons learned through this community case study, analyzing and addressing failed strategies is as equally important to a healthy NIC as is sharing learning from successful interventions. We initially relied on pre-existing relationships and assumptions about how we would work together, rather than making explicit how the NIC would develop, establish norms, understand common processes, and manage changing relationships. We had varied understandings of the depth of campus differences, sometimes resulting in frustrations about the disparate progress on goals. NIC structures require significant engagement with the group, often more intensive than traditional multi-institution organizational structures. They require time to develop and ongoing maintenance in order to advance the work. We continue to reevaluate our model for leadership, climate, diversity, conflict resolution, engagement, decision-making, roles, and data, leading to increased investment in the success of all NIC institutions. Our NIC has evolved from the traditional NIC model to become the Center for the Integration of Research, Teaching and Learning (CIRTL) AGEP NIC model with five key characteristics: (1) A well-specified aim, (2) An understanding of systems, including a variety of contexts and different organizations, (3) A culture and practice of shared leadership and inclusivity, (4) The use of data reflecting different institutional contexts, and (5) The ability to accelerate infrastructure and interventions. We conclude with recommendations for those considering developing a NIC to promote diversity, equity, and inclusion efforts.
RESUMO
Karyotyping couples that have had recurrent miscarriages detects balanced rearrangements in carrier parents who can be offered prenatal cytogenetic analysis to prevent the birth of a subsequent child with an unbalanced rearrangement. In four UK centres, over periods of 5-30 years, balanced rearrangements were found in 406 out of 20,432 parents that had experienced miscarriage (1.9%), but only four unbalanced rearrangements were found after referral for prenatal diagnosis because of a balanced parental translocation ascertained for recurrent miscarriages. At an estimated cost of 3-4 million pounds, these data raise doubts about the cost effectiveness of current policies on the routine karyotyping of couples experiencing repeated miscarriages.
Assuntos
Aborto Habitual/genética , Transtornos Cromossômicos/diagnóstico , Cariotipagem , Diagnóstico Pré-Natal/economia , Aborto Habitual/prevenção & controle , Transtornos Cromossômicos/genética , Análise Custo-Benefício , Feminino , Humanos , Gravidez , Estudos RetrospectivosRESUMO
Complete hydatidiform moles have a diploid chromosome constitution, generally with only paternal genetic material present (diandry). Diandric complete moles are thought to arise either by fertilization of an anucleate oocyte by two spermatozoa or, more commonly, doubling of a single sperm genotype. Molar pregnancies are usually sporadic, and may be accompanied by malignant transformation; however, recurrence is associated with increased risk of further affected pregnancies and of persistent trophoblastic neoplasia or choriocarcinoma. This study presents the first use of preimplantation genotyping to ensure biparental inheritance in a woman presenting with recurrent diandric complete hydatidiform mole. Following an IVF cycle, a single cell from each of 11 embryos was tested by whole genome amplification and genotyping at 16 different simple tandem repeat loci. All embryos showed normal biparental inheritance; one blastocyst was transferred, resulting in the delivery of healthy monozygotic twin girls.
Assuntos
Blastocisto , Mola Hidatiforme/prevenção & controle , Adulto , Feminino , Genótipo , Humanos , Gravidez , RecidivaRESUMO
OBJECTIVE: The role of preoperative localisation of abnormal parathyroid glands remains controversial but is particularly relevant to the management of patients with recurrent or persistent hyperparathyroidism and familial syndromes. We report our experience of the use of selective parathyroid venous sampling (PVS) in the localisation of parathyroid disease in such patients. DESIGN: We report a retrospective 10-year experience (n = 27) of the use of PVS in complicated primary hyperparathyroidism and contrast the use of PVS with neck ultrasound, magnetic resonance imaging (MRI), computed tomography (CT) and sestamibi imaging modalities. RESULTS: In 14 out of 25 patients who underwent surgery PVS results were completely concordant with surgical and histological findings and 88% of patients achieved post-operative cure. Out of 13 patients referred after previous failed surgery, 12 underwent further surgery which was curative in 9. In total PVS yielded useful positive (n = 13) and/or negative information (n = 6) in 19 out of 25 patients undergoing surgery. Using histology as the gold standard, 59% of PVS studies were entirely consistent with histology, as compared with 39% of ultrasound scans, 36% of sestamibi scans and 17% of MRI/CT scans. CONCLUSIONS: PVS is a valuable adjunct to MRI/CT and sestamibi scanning in selected patients with complicated hyperparathyroidism when performed in an experienced unit.
Assuntos
Hiperparatireoidismo/patologia , Glândulas Paratireoides/irrigação sanguínea , Glândulas Paratireoides/patologia , Veia Cava Superior , Adulto , Idoso , Feminino , Humanos , Hiperparatireoidismo/diagnóstico por imagem , Hiperparatireoidismo/cirurgia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Glândulas Paratireoides/diagnóstico por imagem , Hormônio Paratireóideo/sangue , Neoplasias das Paratireoides/diagnóstico por imagem , Neoplasias das Paratireoides/patologia , Neoplasias das Paratireoides/cirurgia , Cuidados Pré-Operatórios , Cintilografia , Compostos Radiofarmacêuticos , Reoperação , Estudos Retrospectivos , Tecnécio Tc 99m Sestamibi , Tomografia Computadorizada por Raios XRESUMO
There has been a huge acceleration in our technical ability to detect variation in the human genome in recent years, and there has been a corresponding effort in clinical diagnostic laboratories to take advantage of this progress for the benefit of patients. There has, however, not been an equivalent increase in our understanding of human genetics and disease, not for lack of effort but due to the far greater complexity of understanding variation than the difficulties of detecting it. This chapter describes how software tools can be used to target clinical genetic diagnostic testing in order to exploit technical and scientific advances both efficiently and cost-effectively, while maximizing clinical utility.
Assuntos
Variações do Número de Cópias de DNA/genética , Testes Genéticos , Adulto , Animais , Feminino , Genoma Humano , Estudo de Associação Genômica Ampla , Humanos , Técnicas de Amplificação de Ácido Nucleico , GravidezRESUMO
OBJECTIVE: To undertake a cost-effectiveness analysis of using microarray comparative genomic hybridisation (array-CGH) as a first-line test versus as a second-line test for the diagnosis of causal chromosomal abnormalities in patients referred to a NHS clinical genetics service in the U.K. with idiopathic learning disability, developmental delay and/or congenital anomalies. METHODS: A cost-effectiveness study was conducted. The perspective is that of a U.K. NHS clinical genetics service provider (with respect to both costs and outcomes). A cohort of patients (n = 1590) referred for array-CGH testing of undiagnosed learning disability and developmental delay by a single NHS regional clinical genetics service (South East Thames Regional Genetics Service), were split into a before-and-after design where 742 patients had array-CGH as a second-line test (before group-comparator intervention) and 848 patients had array-CGH as a first-line test (after group-evaluated intervention). The mean costs were calculated from the clinical genetics testing pathway constructed for each patient including the costs of genetic testing undertaken and clinical appointments scheduled. The outcome was the number of diagnoses each intervention produced so that a mean cost-per-diagnosis could be calculated. The cost effectiveness of the two interventions was calculated as an incremental cost-effectiveness ratio to produce an incremental cost-per-diagnosis (in 2013 GBP). Sensitivity analyses were conducted by altering both costs and effects to check the validity of the outcome. RESULTS: The incremental mean cost of testing patients using the first-line testing strategy was -GBP241.56 (95% CIs -GBP256.93 to -GBP226.19) and the incremental mean gain in the percentage diagnoses was 0.39% (95% CIs -2.73 to 3.51%), which equates to an additional 1 diagnosis per 256 patients tested. This cost-effectiveness study comparing these two strategies estimates that array-CGH first-line testing dominates second-line testing because it was both less costly and as effective. The sensitivity analyses conducted (adjusting both costs and effects) supported the dominance of the first-line testing strategy (i.e. lower cost and as effective). CONCLUSIONS: The first-line testing strategy was estimated to dominate the second-line testing strategy because it was both less costly and as effective. These findings are relevant to the wider UK NHS clinical genetics service, with two key strengths of this study being the appropriateness of the comparator interventions and the direct applicability of the patient cohort within this study and the wider UK patient population.
Assuntos
Aberrações Cromossômicas , Hibridização Genômica Comparativa/economia , Deficiências da Aprendizagem/genética , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Hibridização Genômica Comparativa/métodos , Análise Custo-Benefício , Feminino , Humanos , Lactente , Recém-Nascido , Deficiências da Aprendizagem/diagnóstico , Deficiências da Aprendizagem/economia , Masculino , Pessoa de Meia-Idade , Medicina Estatal/economia , Reino Unido , Adulto JovemRESUMO
Two sisters presented with multiple congenital abnormalities and developmental delay; abnormalities elsewhere in their extended family suggested that their father carried a balanced translocation. G-banded chromosome analysis showed apparently normal karyotypes. Fluorescence in situ hybridisation (FISH) with whole chromosome paints revealed no apparent abnormality in the father. However, further FISH studies, using multiple subtelomeric probes, demonstrated a derivative chromosome 16 in one sister. Subsequent studies showed that her sister also had a derivative 16 which had been inherited in an unbalanced form from their father, who carried a balanced reciprocal translocation between chromosomes 1 and 16. This report describes the detection of this submicroscopic translocation and the clinical findings in the two sisters.
Assuntos
Cromossomos Humanos Par 16/genética , Cromossomos Humanos Par 1/genética , Telômero/genética , Translocação Genética , Adolescente , Saúde da Família , Feminino , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Masculino , LinhagemRESUMO
A group of 103 subjects with a strict diagnosis of autism were tested for deletion of band q11.2 on the long arm of chromosome 22. No deletions were found, indicating that when a patient has been diagnosed with autism using strict and consistent criteria, in the absence of other indications, it is unlikely that this individual will have a 22q11 deletion. Testing for 22q11 deletions is therefore unlikely to be necessary in these patients. Am. J. Med. Genet. (Neuropsychiatr. Genet.) 96:15-17, 2000.
Assuntos
Transtorno Autístico/genética , Deleção Cromossômica , Cromossomos Humanos Par 21 , Adolescente , Transtorno Autístico/diagnóstico , Criança , Pré-Escolar , Feminino , Humanos , Recém-Nascido , MasculinoRESUMO
Menkes disease is a rare X-linked recessive disorder of copper metabolism, characterised by progressive neurological degeneration, abnormal hair and connective tissue manifestations. We report on a girl with classic Menkes disease, carrying a de novo balanced translocation 46,X,t(X;13)(q13.3; q14.3). The translocation breakpoints at Xq13.3 and 13q14.3 coincide with the Menkes disease and Wilson disease loci, respectively.
Assuntos
Proteínas de Transporte de Cátions , Cromossomos Humanos Par 13/genética , Síndrome dos Cabelos Torcidos/genética , Proteínas Recombinantes de Fusão , Translocação Genética , Cromossomo X/genética , Adenosina Trifosfatases/genética , Proteínas de Transporte/genética , ATPases Transportadoras de Cobre , Diagnóstico Diferencial , Evolução Fatal , Feminino , Cabelo/anormalidades , Degeneração Hepatolenticular/genética , Degeneração Hepatolenticular/patologia , Humanos , Hibridização in Situ Fluorescente , Lactente , Cariotipagem , Síndrome dos Cabelos Torcidos/patologiaRESUMO
Seven adult patients with dyspnea resulting from nonmalignant unilateral diaphragmatic paralysis underwent plication of the affected hemidiaphragm. Preoperatively, the patients complained of exertional dyspnea and orthopnea and had a reduced arterial oxygen tension, total lung capacity, vital capacity, expiratory reserve volume, and functional residual capacity. Plication was performed by imbricating the diaphragm in layers through a thoracotomy. After plication there was a significant increase in arterial oxygen tension and all lung volumes except residual volume. The patients' symptoms were improved with plication and a significant decrease was recorded in breathlessness on a visual analogue scale. There were no postoperative complications and mean hospital stay was 12 days.