1.
Biosci Biotechnol Biochem
; 77(12): 2505-9, 2013.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24317072
RESUMO
In the fermentation industry, the traceability of microorganisms during the process is important to ensure safety and efficacy. Ethyl carbamate, a group-2A carcinogen, is produced from ethanol and urea during the storage of food/alcoholic beverages. We isolated non-urea-producing sake yeast car1 mutants carrying a discriminable molecular marker, and demonstrated, by the use of PCR assays, that these mutants are useful for traceability analysis and identification during the sake brewing process.