Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 47
Filtrar
Mais filtros

Base de dados
País/Região como assunto
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
Plant Dis ; 107(5): 1279-1283, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36399003

RESUMO

Boxwood blight can be challenging to detect in the field, especially when symptoms are mild, thus requiring large numbers of plants to be screened. Therefore, a rapid diagnostic assay that can detect the pathogen from large amounts of plant tissue would be useful. Here, we present a crude DNA extraction protocol that is rapid and scalable. The DNA extraction protocol can process large volumes of tough boxwood tissue rapidly without using cetyltrimethylammonium bromide or phenol-chloroform to remove inhibitors. Additionally, to detect the boxwood blight pathogen Calonectria pseudonaviculata, we developed a TaqMan probe to use with previously described PCR primers for a real-time PCR assay. The assay's limit of detection was determined by diluting symptomatic boxwood leaves in nondiseased leaves and by adding spores to nondiseased leaves to simulate diagnostic scenarios. The assay was able to detect the pathogen in symptomatic leaves diluted up to 1 × 104- to 1 × 105-fold in nondiseased leaves and from as low as 1,000 to 10,000 spores added to 1.2 g of nondiseased leaves. The ability to extract DNA from large volumes of plant tissue facilitates screening more plant tissue using the real-time PCR assay without increasing the number of samples to process in the lab.


Assuntos
Buxus , Hypocreales , Reação em Cadeia da Polimerase em Tempo Real , Doenças das Plantas , Hypocreales/genética
2.
Am J Transplant ; 13(8): 2154-60, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23746308

RESUMO

Pancreatic islet transplantation is an attractive therapy for the treatment of insulin-dependent diabetes mellitus. However, the low efficiency of this procedure necessitating sequential transplantations of islets with the use of 2-3 donors for a single recipient, mainly due to the early loss of transplanted islets, hampers its clinical application. Previously, we have shown in mice that a large amount of HMGB1 is released from islets soon after their transplantation and that this triggers innate immune rejection with activation of DC, NKT cells and neutrophils to produce IFN-γ, ultimately leading to the early loss of transplanted islets. Thus, HMGB1 release plays an initial pivotal role in this process; however, its mechanism remains unclear. Here we demonstrate that release of HMGB1 from transplanted islets is due to hypoxic damage resulting from Ca(2+) influx into ß cells through the Na(+) /Ca(2+) exchanger (NCX). Moreover, the hypoxia-induced ß cell damage was prevented by pretreatment with an NCX-specific inhibitor prior to transplantation, resulting in protection and long-term survival of transplanted mouse and human islets when grafted into mice. These findings suggest a novel strategy with potentially great impact to improve the efficiency of islet transplantation in clinical settings by targeting donor islets rather than recipients.


Assuntos
Compostos de Anilina/farmacologia , Diabetes Mellitus Experimental/prevenção & controle , Diabetes Mellitus Tipo 1/imunologia , Rejeição de Enxerto/imunologia , Transplante das Ilhotas Pancreáticas/imunologia , Ilhotas Pancreáticas/imunologia , Éteres Fenílicos/farmacologia , Trocador de Sódio e Cálcio/antagonistas & inibidores , Animais , Diabetes Mellitus Experimental/enzimologia , Diabetes Mellitus Experimental/imunologia , Citometria de Fluxo , Rejeição de Enxerto/tratamento farmacológico , Rejeição de Enxerto/metabolismo , Proteína HMGB1/metabolismo , Humanos , Hipóxia/metabolismo , Hipóxia/patologia , Ilhotas Pancreáticas/efeitos dos fármacos , Ilhotas Pancreáticas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NOD , Camundongos SCID , Trocador de Sódio e Cálcio/metabolismo
3.
Nat Biotechnol ; 19(2): 137-41, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11175727

RESUMO

Recently, several groups have developed green fluorescent protein (GFP)-based Ca(2+) probes. When applied in cells, however, these probes are difficult to use because of a low signal-to-noise ratio. Here we report the development of a high-affinity Ca(2+) probe composed of a single GFP (named G-CaMP). G-CaMP showed an apparent K(d) for Ca(2+) of 235 nM. Association kinetics of Ca(2+) binding were faster at higher Ca(2+) concentrations, with time constants decreasing from 230 ms at 0.2 microM Ca(2+) to 2.5 ms at 1 microM Ca(2+). Dissociation kinetics (tau approximately 200 ms) are independent of Ca(2+) concentrations. In HEK-293 cells and mouse myotubes expressing G-CaMP, large fluorescent changes were observed in response to application of drugs or electrical stimulations. G-CaMP will be a useful tool for visualizing intracellular Ca2+ in living cells. Mutational analysis, together with previous structural information, suggests the residues that may alter the fluorescence of GFP.


Assuntos
Cálcio/análise , Cálcio/metabolismo , Proteínas Luminescentes/metabolismo , Trifosfato de Adenosina/farmacologia , Sequência de Aminoácidos , Animais , Carbacol/farmacologia , Linhagem Celular , Galinhas , Ácido Edético/farmacologia , Proteínas de Fluorescência Verde , Humanos , Indicadores e Reagentes , Ionomicina/farmacologia , Rim , Cinética , Proteínas Luminescentes/análise , Proteínas Luminescentes/genética , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Quinase de Cadeia Leve de Miosina/genética , Quinase de Cadeia Leve de Miosina/metabolismo , Miosinas/química , Miosinas/genética , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Proteínas Recombinantes/análise , Proteínas Recombinantes/metabolismo , Transfecção
4.
Biochim Biophys Acta ; 1451(1): 132-40, 1999 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-10446395

RESUMO

The molecular mechanism of Ca(2+) release by myotoxin a (MTYX), a polypeptide toxin isolated from the venom of prairie rattlesnakes (Crotalus viridis viridis), was investigated in the heavy fraction of sarcoplasmic reticulum (HSR) of rabbit skeletal muscles. [(125)I]MYTX bound to four HSR proteins (106, 74, 53 and 30 kDa) on polyvinylidene difluoride (PVDF) membrane. DIDS, 4, 4'-diisothiocyanatostilbene-2,2'-disulfonic acid, bound predominantly to 30 kDa protein on the PVDF membrane, the molecular weight of which was similar to one of the MYTX binding proteins. The maximum (45)Ca(2+) release induced by caffeine (30 mM) was further increased in the presence of MYTX (10 microM) or DIDS (30 microM), whereas that induced by DIDS (30 microM) was not affected by MYTX (10 microM). MYTX inhibited [(3)H]DIDS binding to HSR in a concentration-dependent manner. Furthermore, [(125)I]MYTX binding to 30 kDa protein was inhibited by DIDS in a concentration-dependent manner. These results suggest that MYTX and DIDS release Ca(2+) from HSR in a common mechanism. The 30 kDa protein may be a target protein for the Ca(2+) releasing action of MYTX and DIDS.


Assuntos
Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico/farmacologia , Cálcio/metabolismo , Venenos de Crotalídeos/farmacologia , Proteínas Musculares/análise , Músculo Esquelético/efeitos dos fármacos , Animais , Sítios de Ligação , Cafeína/farmacologia , Bloqueadores dos Canais de Cálcio/farmacologia , Eletroforese em Gel de Poliacrilamida/métodos , Proteínas Musculares/química , Proteínas Musculares/metabolismo , Músculo Esquelético/metabolismo , Coelhos , Retículo Sarcoplasmático/efeitos dos fármacos
5.
Biochim Biophys Acta ; 1294(2): 177-82, 1996 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-8645736

RESUMO

Quinolidomicin A1, a 60-membered macrolide purified from an actinomycete Micromonospora sp. markedly induced 45Ca2+ release from the heavy fraction of skeletal muscle sarcoplasmic reticulum (HSR), but induced only slightly from the light fraction of sarcoplasmic reticulum (LSR), showing a lack of the ionophoretic activity even at a high concentration (300 microM). This was also confirmed by measuring the 45Ca2+ transport activity of quinolidomicin A1 across an organic solvent barrier. Quinolidomicin A1 (3-300 microM) increased 45Ca2+ release from HSR with an EC50 value of approx. 20 microM. The potency of quinolidomicin A1 was approx. 100-fold higher than that of caffeine. The bell-shaped profile of Ca2+ dependence for quinolidomicin A1 was different from that for caffeine. Blockers of Ca2+ release channels such as Mg2+ (10 mM), procaine (10 mM) and ruthenium red (10 microM) partially blocked quinolidomicin A1 (30 microM)-induced 45Ca2+ release from HSR. At 0 degrees C, quinolidomicin A1-induced 45Ca2+ release was ascertained not to be due to the inhibition of Ca2+ ATPase by the ATPase assay. Quinolidomicin A1 potentiated [3H]ryanodine binding to HSR with a decrease in KD but without a change in Bmax. These results suggest that quinolidomicin A1-induced Ca2+ release from HSR is consisted of two components, which are both sensitive and insensitive to blockers of Ca2+ release channels, and that the former component is associated with the ryanodine receptor.


Assuntos
Antibacterianos/farmacologia , Cálcio/metabolismo , Macrolídeos , Músculo Esquelético/metabolismo , Retículo Sarcoplasmático/metabolismo , Actinomycetales , Análise de Variância , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antibióticos Antineoplásicos/farmacologia , Transporte Biológico/efeitos dos fármacos , Cafeína/farmacologia , Radioisótopos de Cálcio , Cinética , Estrutura Molecular , Coelhos , Rianodina/metabolismo , Retículo Sarcoplasmático/efeitos dos fármacos
6.
Artigo em Inglês | MEDLINE | ID: mdl-26737853

RESUMO

Our emotional healthcare system is designed to cope with users' negative emotions in daily life. To make the system more intelligent, we integrated emotion recognition by facial expression to provide appropriate services based on user's current emotional state. Our emotion recognition by facial expression has confusion issue to recognize some positive, neutral and negative emotions that make the emotional healthcare system provide a relaxation service even though users don't have negative emotions. Therefore, to increase the effectiveness of the system to provide the relaxation service, we integrate stress detection from ECG signal. The stress detection might be able to address the confusion issue of emotion recognition by facial expression to provide the service. Indeed, our results show that integration of stress detection increases the effectiveness and efficiency of the emotional healthcare system to provide services.


Assuntos
Eletrocardiografia/métodos , Emoções/fisiologia , Estresse Psicológico , Expressão Facial , Humanos , Saúde Mental , Processamento de Sinais Assistido por Computador , Estresse Psicológico/diagnóstico , Estresse Psicológico/fisiopatologia
7.
Cell Death Differ ; 22(8): 1260-74, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25571974

RESUMO

Axonal transport is critical for neuronal development and function, and defective axonal transport has been implicated in neurodegenerative diseases. However, how axonal transport is regulated, or how defective transport leads to neuronal degeneration, remains unclear. Here, we report that c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1, also known as JNK-interacting protein 3 (JIP3)) and JNK-associated leucine zipper protein (JLP) are essential for postnatal brain development. Mice with a double-knockout (dKO) in Jsap1 and Jlp in the dorsal telencephalon developed progressive neuron loss. Using a primary neuron culture system with induced disruption of targeted genes, combined with gene rescue experiments, we show that JSAP1 and JLP regulate kinesin-1-dependent axonal transport with functional redundancy. We also show that the binding of JSAP1 and JLP to kinesin-1 heavy chain is crucial for interactions between kinesin-1 and microtubules. Furthermore, we describe a molecular mechanism by which defective kinesin-1-dependent axonal transport in Jsap1:Jlp dKO neurons causes axonal degeneration and subsequent neuronal death. JNK hyperactivation because of increased intra-axonal Ca(2+) in the Jsap1:Jlp dKO neurons was found to mediate both the axonal degeneration and neuronal death, in cooperation with the Ca(2+)-dependent protease calpain. Our results indicate that axonal JNK may relocate to the nucleus in a dynein-dependent manner, where it activates the transcription factor c-Jun, resulting in neuronal death. Taken together, our data establish JSAP1 and JLP as positive regulators of kinesin-1-dependent axonal transport, which prevents neuronal degeneration.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Transporte Axonal/fisiologia , Axônios/metabolismo , Cinesinas/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/genética , Animais , Transporte Axonal/genética , Axônios/fisiologia , Células Cultivadas , Cinesinas/genética , Masculino , Camundongos , Camundongos Knockout , Proteínas do Tecido Nervoso/genética
8.
Br J Pharmacol ; 113(1): 233-9, 1994 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-7812616

RESUMO

1. Myotoxin alpha (MYTX), a polypeptide toxin purified from the venom of prairie rattlesnakes (Crotalus viridis viridis) induced Ca2+ release from the heavy fraction (HSR) but not the light fraction of skeletal sarcoplasmic reticulum at concentrations higher than 1 microM, followed by spontaneous Ca2+ reuptake by measuring extravesicular Ca2+ concentrations using the Ca2+ electrode. 2. The rate of 45Ca2+ release from HSR vesicles was markedly accelerated by MYTX in a concentration-dependent manner in the range of concentrations between 30 nM and 10 microM, indicating the most potent Ca2+ releaser in HSR. 3. The Ca2+ dependency of MYTX-induced 45Ca2+ release has a bell-shaped profile but it was quite different from that of caffeine, an inducer of Ca(2+)-induced Ca2+ release. 4. 45Ca2+ release induced by MYTX was remarkable in the range of pCa between 8 and 3, whereas that by caffeine was prominent in the range of pCa, i.e., between 7 and 5.5. 5. MYTX-induced 45Ca2+ release consists of both early and late components. The early component caused by MYTX at low concentrations (30-300 nM) completed within 20 s, while the late component induced by it at higher concentrations (> 0.3 microM) was maintained for at least 1 min. 6. Both the components were almost completely inhibited by inhibitors of Ca2+ such as Mg2+, ruthenium red and spermine. 7. 45Ca2+ release induced by caffeine or beta,gamma-methyleneadenosine 5'-triphosphate (AMP-PCP) was completely inhibited by high concentrations of procaine. Procaine abolished the early component but not the late one, suggesting that at least the early component is mediated through Ca(2+)-induced Ca2+ release channels. 8. On the basis of these results, the character of Ca2+ release induced by MYTX was quite different from that caused by caffeine or AMP-PCP, suggesting that MYTX induces Ca2+ release having novel properties in HSR. MYTX is the first polypeptide Ca2+ inducer and has become a useful pharmacological tool for clarifying the mechanism of Ca2+ release from skeletal muscle SR.


Assuntos
Cálcio/metabolismo , Venenos de Crotalídeos/farmacologia , Retículo Sarcoplasmático/metabolismo , Trifosfato de Adenosina/análogos & derivados , Trifosfato de Adenosina/antagonistas & inibidores , Trifosfato de Adenosina/farmacologia , Animais , ATPase de Ca(2+) e Mg(2+)/metabolismo , Cafeína/antagonistas & inibidores , Cafeína/farmacologia , Radioisótopos de Cálcio , Venenos de Crotalídeos/antagonistas & inibidores , Venenos de Crotalídeos/isolamento & purificação , Técnicas In Vitro , Eletrodos Seletivos de Íons , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Músculo Esquelético/ultraestrutura , Procaína/farmacologia , Coelhos , Rianodina/farmacocinética , Retículo Sarcoplasmático/efeitos dos fármacos , Retículo Sarcoplasmático/enzimologia
9.
J Thorac Cardiovasc Surg ; 78(1): 7-11, 1979 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-449387

RESUMO

A new surgical method for repair of anomalous origin of the left coronary artery from the pulmonary trunk is described. The principle of the proposed technique is to transmit the oxygenated blood to the anomalous left coronary artery through surgically created aortopulmonary window and the internal tunnel created in the main pulmonary trunk. A 2-year-old boy in whom this anomaly was associated with mitral regurgitation caused by papillary muscle dysfunction was successfully treated by this new surgical method, and the deformed mitral valve was concomitantly replaced by a Hancock porcine xenograft.


Assuntos
Aorta/cirurgia , Anomalias dos Vasos Coronários/cirurgia , Artéria Pulmonar/anormalidades , Angiocardiografia , Aortografia , Bioprótese , Pré-Escolar , Anomalias dos Vasos Coronários/complicações , Anomalias dos Vasos Coronários/diagnóstico por imagem , Próteses Valvulares Cardíacas , Humanos , Masculino , Métodos , Insuficiência da Valva Mitral/complicações , Insuficiência da Valva Mitral/cirurgia , Pericárdio/transplante , Artéria Pulmonar/diagnóstico por imagem , Artéria Pulmonar/cirurgia , Transplante Autólogo
10.
Brain Res ; 911(2): 141-5, 2001 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-11511381

RESUMO

A serotonin (5-hydroxytryptamine, 5-HT)-releasing drug, p-chloroamphetamine elicited decreases in 5-HT levels in the mouse frontal cortex. 5-HT reduction elicited by p-chloroamphetamine was inhibited by the 5-HT(2A/2B/2C) receptor antagonist, LY 53857 and the 5-HT(2A) receptor antagonist, ketanserin. However, the 5-HT(2B/2C) receptor antagonist, SB 206553, enhanced it. LY 53857 and ketanserin can inhibit hyperthermia elicited by p-chloroamphetamine, although SB 206553 enhances it. The effects of the 5-HT(2) receptor antagonists on neurotoxicity are very similar to those on hyperthermia. Since hyperthermia facilitates neurotoxicity induced by amphetamine analogue, these 5-HT(2) receptor antagonists may modify 5-HT depletion induced by p-chloroamphetamine through responses to body temperature.


Assuntos
Febre/induzido quimicamente , Lobo Frontal/efeitos dos fármacos , Neurotoxinas/farmacologia , Receptores de Serotonina/efeitos dos fármacos , Serotoninérgicos/farmacologia , Serotonina/deficiência , p-Cloroanfetamina/farmacologia , Animais , Temperatura Corporal/efeitos dos fármacos , Temperatura Corporal/fisiologia , Química Encefálica/efeitos dos fármacos , Química Encefálica/fisiologia , Interações Medicamentosas/fisiologia , Febre/metabolismo , Febre/fisiopatologia , Lobo Frontal/metabolismo , Lobo Frontal/fisiopatologia , Masculino , Camundongos , Degeneração Neural/induzido quimicamente , Degeneração Neural/metabolismo , Degeneração Neural/fisiopatologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurotoxinas/metabolismo , Receptores de Serotonina/metabolismo , Antagonistas da Serotonina/farmacologia
11.
Eur J Pharmacol ; 403(3): 225-8, 2000 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-10973623

RESUMO

The effects of a serotonin (5-hydroxytryptamine, 5-HT)-releasing drug, p-chloroamphetamine (PCA), on body temperature were investigated in mice. PCA induced hyperthermia in mice. PCA-induced hyperthermia was inhibited by the 5-HT(2A/2B/2C) receptor antagonist, 4-isopropyl-7-methyl-9-(2-hydroxy-1-methyl-propoxycarbonyl)-4,6A,7 , 8,9,10,10A-octahydro-indolo[4,3-FG]quinolone maleate (LY53857). The 5-HT(2A) receptor antagonist, ketanserin, reduced the PCA-induced hyperthermia, while the 5-HT(2B/2C) receptor antagonist, N-3-pyridinyl-3,5-dihydro-5-methyl-benzo[1,2-b:4, 5-b']dipyrrole-1(2H)-carboxamide (SB 206553), enhanced it. LY 53857, ketanserin and SB 206553 did not affect hyperactivity in mice treated with PCA. These results suggest that PCA-induced hyperthermia in mice is mediated by 5-HT(2A) receptors and is not related to changes in locomotor activity.


Assuntos
Febre/fisiopatologia , Receptores de Serotonina/fisiologia , Serotoninérgicos/farmacologia , p-Cloroanfetamina/farmacologia , Animais , Regulação da Temperatura Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ergolinas/farmacologia , Febre/induzido quimicamente , Indóis/farmacologia , Ketanserina/farmacologia , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Piridinas/farmacologia , Receptores de Serotonina/efeitos dos fármacos , Antagonistas da Serotonina/farmacologia
12.
Eur J Pharmacol ; 430(2-3): 265-8, 2001 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-11711040

RESUMO

Serotonergic and dopaminergic involvement in hyperthermia induced by a serotonin (5-hydroxytryptamine, 5-HT)-releasing drug, p-chloroamphetamine, was investigated in mice. Neither the 5-HT transporter inhibitor fluoxetine nor the 5-HT depleter p-chlorophenylalanine affected p-chloroamphetamine-induced hyperthermia. The dopamine depleter alpha-methyl-p-tyrosine significantly reduced p-chloroamphetamine-induced hyperthermia. The dopamine D(1) receptor antagonist 7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine (SCH 23390) antagonized p-chloroamphetamine-induced hyperthermia, although the dopamine D(2) receptor antagonist sulpiride was without effect. These results indicate that p-chloroamphetamine-induced hyperthermia in mice is mediated by dopamine release followed by activation of the dopamine D(1) receptor.


Assuntos
Febre/fisiopatologia , Receptores Dopaminérgicos/fisiologia , Receptores de Serotonina/fisiologia , Serotoninérgicos/farmacologia , p-Cloroanfetamina/farmacologia , Animais , Benzazepinas/farmacologia , Temperatura Corporal/efeitos dos fármacos , Antagonistas de Dopamina/farmacologia , Inibidores Enzimáticos/farmacologia , Fenclonina/farmacologia , Febre/induzido quimicamente , Febre/metabolismo , Fluoxetina/farmacologia , Masculino , Camundongos , Serotonina/metabolismo , Antagonistas da Serotonina/farmacologia , Inibidores Seletivos de Recaptação de Serotonina/farmacologia , Sulpirida/farmacologia , Fatores de Tempo , alfa-Metiltirosina/farmacologia
13.
Eur J Pharmacol ; 268(1): R1-2, 1994 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-7925605

RESUMO

Myotoxin alpha from prairie rattlesnakes induced Ca2+ release from the heavy fraction of sarcoplasmic reticulum at submicromolar concentrations. 125I-Labeled myotoxin alpha (125I-myotoxin alpha) specifically bound to the heavy fraction. Fractionation of the solubilized heavy fraction with a spermine-agarose column gave purified calsequestrin as a major binding protein of 125I-myotoxin alpha. We have first indicated that calsequestrin is a target protein for Ca(2+)-releasing action of myotoxin alpha. Calsequestrin probably plays a key role in physiological Ca2+ release from sarcoplasmic reticulum.


Assuntos
Cálcio/metabolismo , Calsequestrina/metabolismo , Venenos de Crotalídeos/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Crotalus , Ligação Proteica
14.
Leuk Lymphoma ; 42(4): 761-74, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11697507

RESUMO

Two new human myeloma cell lines were established from pleural effusion and bone marrow malignant cells derived from a single patient, who manifested hyperammonemia associated with multiple myeloma, and these were characterized. Both lines possess t(11;14)(q13;q32) and t(8;14)(q24;q32) reciprocal translocations and overexpress cyclin D1, but not c-myc. Human myeloma lines including these new lines produced and secreted excess ammonia into culture medium more than non-myelomatous hematological cell lines. In addition, these two lines were revealed to have high surface CD7 expression correlated with relatively high mRNA expression by MP-RT-PCR. Among 8 human myeloma lines, half of them revealed significant surface expression of CD7 and a positive correlation between expression levels of protein and message. CD7 message was also detected in surface negative lines. Consequently, there may be posttranslational regulation of the CD7 molecule, whose cellular biological role in expressing cells has not been elucidated.


Assuntos
Antígenos CD7/metabolismo , Cromossomos Humanos Par 11 , Cromossomos Humanos Par 14 , Cromossomos Humanos Par 8 , Mieloma Múltiplo/patologia , Translocação Genética , Células Tumorais Cultivadas/citologia , Adulto , Amônia/metabolismo , Células da Medula Óssea/patologia , Ciclina D1/metabolismo , Humanos , Hiperamonemia/etiologia , Hiperamonemia/patologia , Masculino , Mieloma Múltiplo/complicações , Mieloma Múltiplo/genética , Derrame Pleural Maligno/patologia , Proteínas Proto-Oncogênicas c-myc/metabolismo , Células Tumorais Cultivadas/imunologia , Células Tumorais Cultivadas/metabolismo
15.
Scand J Work Environ Health ; 24(5): 392-7, 1998 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9869311

RESUMO

OBJECTIVES: This study explored the high prevalence of pleural plaques in the town of Matsubase in Kumamoto, Japan. METHODS: Small-size chest X-ray film was used for screening, and all persons with pleural plaques were confirmed by computed tomography (CT). The prevalence rate of pleural plaques in the 4 districts of Matsubase and its surrounding towns and cities were also examined. The age-adjusted mortality rate for lung cancer in this town was compared with that of its surrounding towns and cities. RESULTS: Pleural plaques were found in 1357 persons (724 men and 633 women) among the inhabitants who were more than 20 years of age in Matsubase between 1988 and 1993. CT scans ascertained 938 cases with pleural plaques among the 11 14 persons who participated. Thus at least 9.5% of the inhabitants over 20 years of age in this town had pleural plaques. The neighboring towns had a higher rate than the more distant towns. A large-scale open-cast asbestos mine and mill had been in operation in Matsubase between 1883 and 1970. Mineral analysis revealed anthophyllite fibers. Most of the plaques were found in persons who had never worked in the mine or mill. CONCLUSIONS: The high prevalence of pleural plaques in Matsubase was due to anthophyllite exposure, mainly environmental. No mesotheliomas were found, however. These findings agree with those from an earlier study from Finland.


Assuntos
Amianto/efeitos adversos , Exposição Ambiental/efeitos adversos , Doenças Pleurais/epidemiologia , Doenças Pleurais/etiologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/isolamento & purificação , Amianto/isolamento & purificação , Feminino , Humanos , Japão/epidemiologia , Neoplasias Pulmonares/mortalidade , Masculino , Pessoa de Meia-Idade , Doenças Pleurais/diagnóstico por imagem , Prevalência , Radiografia
16.
J Pediatr Surg ; 27(7): 882-4, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1640338

RESUMO

This is a report of our experience with 22 cases of large unruptured omphaloceles treated by amnion inversion during the period 1973 through 1990. The method is characterized by three stages: (1) a silastic sheet is sutured directly to the skin around the amniotic membrane, under local anaesthesia, without dissection between the skin and the amnion; (2) the reduction of herniated viscera into the abdominal cavity is achieved by squeezing the sheeting using a specially modified stapler; and (3) the amniotic membrane is preserved intact, and inverted into the abdominal cavity at the time of abdominal wall closure. Of the 22 infants, 19 survived with satisfactory results. Two patients died of multiple associated anomalies, and the remaining patient died of sepsis arising at the time of the final abdominal closure. This procedure has proved to be effective and safe for high-risk patients with congenital heart diseases, anal atresia, tracheoesophageal fistula, or bronchial stenosis and prematurity. The practical aspects of the procedure, as well as its advantages and pitfalls, are illustrated.


Assuntos
Âmnio/cirurgia , Hérnia Umbilical/cirurgia , Técnicas de Sutura , Feminino , Humanos , Recém-Nascido , Masculino , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/mortalidade , Procedimentos Cirúrgicos Operatórios/métodos , Resultado do Tratamento
17.
J Pediatr Surg ; 30(1): 97-100, 1995 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-7722841

RESUMO

Based on a review of operative notes of recurrent inguinal hernia cases from the authors' primary series, a surgical technique modified from the Marcy repair is described. With this technique, emphasis is placed on preservation of the intact internal spermatic fascia and reduction in the size of the internal inguinal ring. Through the inguinal approach, the sleeve-like extension of the internal spermatic fascia is incised longitudinally along the cord and up to the internal ring. The cord structures are dissected off the sac, and as much of the fascial tissue as possible is preserved intact. Both edges of the fascial defect are approximated with an unabsorbable suture; great care is taken to not penetrate the wall of the sac. The same suture is then used for high ligation, via a stay suture placed on the transversalis fascia on the other side of the neck, to reduce the size of the internal ring. The technique can be used in premature babies who have a flimsy, easily torn sac, and in some cases of giant hernia with a widely dilated internal inguinal ring, if the direct wall integrity remains adequate.


Assuntos
Fasciotomia , Hérnia Inguinal/cirurgia , Cordão Espermático/cirurgia , Técnicas de Sutura , Ducto Deferente/cirurgia , Adolescente , Criança , Pré-Escolar , Hérnia Inguinal/patologia , Humanos , Lactente , Recém-Nascido , Ligadura , Masculino , Recidiva , Procedimentos Cirúrgicos Operatórios/métodos
18.
J Pediatr Surg ; 28(12): 1622-5, 1993 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8301514

RESUMO

Thymolipoma, a rare benign mediastinal tumor, was found in a 6-year-old boy. Computed tomography scans, magnetic resonance imaging, and angiography of internal thoracic artery confirmed the diagnosis before surgery.


Assuntos
Lipoma/epidemiologia , Timo/patologia , Neoplasias do Timo/epidemiologia , Tecido Adiposo/patologia , Criança , Diagnóstico por Imagem , Humanos , Lipoma/diagnóstico , Lipoma/cirurgia , Masculino , Timoma/diagnóstico , Hiperplasia do Timo/diagnóstico , Neoplasias do Timo/diagnóstico , Neoplasias do Timo/cirurgia
19.
J Pediatr Surg ; 28(6): 833-7, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8331514

RESUMO

A 27-year-old mother was diagnosed by prenatal ultrasonography as having triplets at gestational age 32 weeks. Following cesarean section at 37 weeks, a pair of female babies were noted for the first time to be joined by a common pelvis with three lower limbs. They had separate upper gastrointestinal tracts, which joined in the distal ileum, leading to a common colon, rectum, and a single anus. Each twin had a functioning kidney, with a single ureter leading to a common bladder. A common urethra originating from the bladder neck ran into the urogenital sinus of one baby. Prior to the surgical separation, placement of four tissue expanders and 20 pneumoperitoneums were performed, in order to stretch the parietes for easier approximation of the wound edges. At 13 months of age, separation was performed, requiring 17 hours. The skin and musculature from the conjoined third leg was used as a fillet for abdominal wall closure in each patient. One infant was given the distal half of the colon and an entire anus with a temporary jejunostomy, and the right half of the bladder with the urethra. The other infant was given the proximal half of the colon with a permanent colostomy, and the left half of the bladder with permanent cystostomy using appendiceal pedicle graft (Mitrofanoff's procedure). This is the 10th case of surgical separation in ischiopagus tripus twins reported in the literature, and the seventh successful separation with both patients alive.


Assuntos
Qualidade de Vida , Gêmeos Unidos/cirurgia , Feminino , Humanos , Lactente , Intestino Grosso/anormalidades , Deformidades Congênitas dos Membros , Planejamento de Assistência ao Paciente , Ossos Pélvicos/anormalidades , Pneumoperitônio Artificial , Expansão de Tecido
20.
J Dermatol ; 22(1): 62-7, 1995 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-7897028

RESUMO

A 74-year-old Japanese male had developed generalized erythroderma and rapid growth of multiple verrucous lesions over the entire surface of his face, trunk, and extremities three months before he was seen. Histologically seborrheic keratoses were revealed. Laboratory examinations showed peripheral leukocytosis with atypical lymphocytes and high levels of IgE and IgG. On the basis of these clinical and histopathologic findings, we diagnosed the patient as having Leser-Trélat sign associated with Sézary syndrome. The erythroderma subsided after administration of oral predonisone, and no new formations of seborrheic keratosis were observed. However, because of subsequent aggravation of the generalized erythroderma, we administered chemotherapy. Six months after the initial examination, lung cancer was found, and the patient subsequently died of respiratory and renal failure.


Assuntos
Ceratose Seborreica/patologia , Síndromes Paraneoplásicas , Síndrome de Sézary/patologia , Neoplasias Cutâneas/patologia , Idoso , Carcinoma de Células Escamosas/patologia , Dermatite Esfoliativa/patologia , Seguimentos , Humanos , Neoplasias Pulmonares/patologia , Masculino , Segunda Neoplasia Primária/patologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA