RESUMO
Chronic respiratory diseases often necessitate lung transplantation due to irreversible damage. Organ engineering offers hope through stem cell-based organ generation. However, the crucial sterilization step in scaffold preparation poses challenges. This study conducted a systematic review of studies that analysed the extracellular matrix (ECM) conditions of decellularised lungs subjected to different sterilisation processes. A search was performed for articles published in the PubMed, Web of Sciences, Scopus, and SciELO databases according to the PRISMA guidelines. Overall, five articles that presented positive results regarding the effectiveness of the sterilisation process were selected, some of which identified functional damage in the ECM. Was possible concluded that regardless of the type of agent used, physical or chemical, all of them demonstrated that sterilisation somehow harms the ECM. An ideal protocol has not been found to be fully effective in the sterilisation of pulmonary scaffolds for use in tissue and/or organ engineering.
Assuntos
Matriz Extracelular , Pulmão , Esterilização , Alicerces Teciduais , Esterilização/métodos , Humanos , Engenharia Tecidual/métodos , AnimaisRESUMO
O objetivo do estudo foi determinar a prevalência de depressão em idosos que freqüentam centros de convivência. Foi realizado estudo descritivo transversal, de fevereiro a julho de 2001, com idosos de idade igual ou acima de 60 anos, provenientes de centros de convivência de Taguatinga, Brasília, DF. A amostra foi composta de 118 idosos, que foram distribuídos em faixas etárias com intervalos de cinco anos e responderam à Escala de Depressão Geriátrica de Yesavage, versão simplificada com 15 perguntas. Foram realizadas análises de variância entre as faixas etárias, teste de Tukey, com intervalo de confiança de 95 por cento. Houve predominância do sexo feminino (90 por cento) e a maioria tinha entre 60 e 64 anos (31 por cento). A depressão foi identificada em 36 idosos (31 por cento), 4 por cento apresentaram depressão grave, e desses, todos na faixa entre 60 e 64 anos (14 por cento do grupo). Recomenda-se a criação de programas nacionais com o objetivo de diminuir sintomas depressivos entre os idosos em centros de convivência.
The objective of the study was to establish the prevalence rate of depression among the elderly population (those 60 and older) who frequent community centers. From February to July of 2001, a cross-sectional survey was conducted with elderly people from community centers in Taguatinga, Brasilia, FD (Federal District). The sample included 118 elderly people, distributed in five-year age brackets, who responded to a simplified version of the Yesavage Geriatric Depression Scale with 15 questions. Anova and the Tukey test were performed to analyze differences between the age groups with 95 percent confidence intervals. The sample was predominantly female (90 percent) with the majority reporting being in the age bracket of 60 to 64 years old (31 percent). Depression was reported by 36 respondents (31 percent) and severe depression was reported by 4 percent of those interviewed, all of whom were in the age bracket of 60 to 64 (representing 14 percent of this group). National programs should be developed in community centers focused on decreasing depression among the elderly population.