Climatic effects on the synchrony and stability of temperate headwater invertebrates over four decades.

Important clues about the ecological effects of climate change can arise from understanding the influence of other Earth-system processes on ecosystem dynamics but few studies span the inter-decadal timescales required. We, therefore, examined how variation in annual weather patterns associated with the North Atlantic Oscillation (NAO) over four decades was linked to synchrony and stability in a metacommunity of stream invertebrates across multiple, contrasting headwaters in central Wales (UK). Prolonged warmer and wetter conditions during positive NAO winters appeared to synchronize variations in population and community composition among and within streams thereby reducing stability across levels of ecological organization. This climatically mediated synchronization occurred in all streams irrespective of acid-base status and land use, but was weaker where invertebrate communities were more functionally diverse. Wavelet linear models indicated that variation in the NAO explained up to 50% of overall synchrony in species abundances at a timescale of 4-6 years. The NAO appeared to affect ecological dynamics through local variations in temperature, precipitation and discharge, but increasing hydrochemical variability within sites during wetter winters might have contributed. Our findings illustrate how large-scale climatic fluctuations generated over the North Atlantic can affect population persistence and dynamics in inland freshwater ecosystems in ways that transcend local catchment character. Protecting and restoring functional diversity in stream communities might increase their stability against warmer, wetter conditions that are analogues of ongoing climate change. Catchment management could also dampen impacts and provide options for climate change adaptation.

Bending the Curve of Global Freshwater Biodiversity Loss: An Emergency Recovery Plan.

Despite their limited spatial extent, freshwater ecosystems host remarkable biodiversity, including one-third of all vertebrate species. This biodiversity is declining dramatically: Globally, wetlands are vanishing three times faster than forests, and freshwater vertebrate populations have fallen more than twice as steeply as terrestrial or marine populations. Threats to freshwater biodiversity are well documented but coordinated action to reverse the decline is lacking. We present an Emergency Recovery Plan to bend the curve of freshwater biodiversity loss. Priority actions include accelerating implementation of environmental flows; improving water quality; protecting and restoring critical habitats; managing the exploitation of freshwater ecosystem resources, especially species and riverine aggregates; preventing and controlling nonnative species invasions; and safeguarding and restoring river connectivity. We recommend adjustments to targets and indicators for the Convention on Biological Diversity and the Sustainable Development Goals and roles for national and international state and nonstate actors.

A catchment-scale perspective of plastic pollution.

Plastic pollution is distributed across the globe, but compared with marine environments, there is only rudimentary understanding of the distribution and effects of plastics in other ecosystems. Here, we review the transport and effects of plastics across terrestrial, freshwater and marine environments. We focus on hydrological catchments as well-defined landscape units that provide an integrating scale at which plastic pollution can be investigated and managed. Diverse processes are responsible for the observed ubiquity of plastic pollution, but sources, fluxes and sinks in river catchments are poorly quantified. Early indications are that rivers are hotspots of plastic pollution, supporting some of the highest recorded concentrations. River systems are also likely pivotal conduits for plastic transport among the terrestrial, floodplain, riparian, benthic and transitional ecosystems with which they connect. Although ecological effects of micro- and nanoplastics might arise through a variety of physical and chemical mechanisms, consensus and understanding of their nature, severity and scale are restricted. Furthermore, while individual-level effects are often graphically represented in public media, knowledge of the extent and severity of the impacts of plastic at population, community and ecosystem levels is limited. Given the potential social, ecological and economic consequences, we call for more comprehensive investigations of plastic pollution in ecosystems to guide effective management action and risk assessment. This is reliant on (a) expanding research to quantify sources, sinks, fluxes and fates of plastics in catchments and transitional waters both independently as a major transport routes to marine ecosystems, (b) improving environmentally relevant dose-response relationships for different organisms and effect pathways, (c) scaling up from studies on individual organisms to populations and ecosystems, where individual effects are shown to cause harm and; (d) improving biomonitoring through developing ecologically relevant metrics based on contemporary plastic research.

Lifting the veil: richness measurements fail to detect systematic biodiversity change over three decades.

While there is widespread recognition of human involvement in biodiversity loss globally, at smaller spatial extents, the effects are less clear. One reason is that local effects are obscured by the use of summary biodiversity variables, such as species richness, that provide only limited insight into complex biodiversity change. Here, we use 30 yr of invertebrate data from a metacommunity of 10 streams in Wales, UK, combined with regional surveys, to examine temporal changes in multiple biodiversity measures at local, metacommunity, and regional scales. There was no change in taxonomic or functional α-diversity and spatial ß-diversity metrics at any scale over the 30-yr time series, suggesting a relative stasis in the system and no evidence for on-going homogenization. However, temporal changes in mean species composition were evident. Two independent approaches to estimate species niche breadth showed that compositional changes were associated with a systematic decline in mean community specialization. Estimates of species-specific local extinction and immigration probabilities suggested that this decline was linked to lower recolonization rates of specialists, rather than greater local extinction rates. Our results reveal the need for caution in implying stasis from patterns in α-diversity and spatial ß-diversity measures that might mask non-random biodiversity changes over time. We also show how different but complementary approaches to estimate niche breadth and functional distinctness of species can reveal long-term trends in community homogenization likely to be important to conservation and ecosystem function.

Beyond cool: adapting upland streams for climate change using riparian woodlands.

Managed adaptation could reduce the risks of climate change to the world's ecosystems, but there have been surprisingly few practical evaluations of the options available. For example, riparian woodland is advocated widely as shade to reduce warming in temperate streams, but few studies have considered collateral effects on species composition or ecosystem functions. Here, we use cross-sectional analyses at two scales (region and within streams) to investigate whether four types of riparian management, including those proposed to reduce potential climate change impacts, might also affect the composition, functional character, dynamics and energetic resourcing of macroinvertebrates in upland Welsh streams (UK). Riparian land use across the region had only small effects on invertebrate taxonomic composition, while stable isotope data showed how energetic resources assimilated by macroinvertebrates in all functional guilds were split roughly 50:50 between terrestrial and aquatic origins irrespective of riparian management. Nevertheless, streams draining the most extensive deciduous woodland had the greatest stocks of coarse particulate matter (CPOM) and greater numbers of 'shredding' detritivores. Stream-scale investigations showed that macroinvertebrate biomass in deciduous woodland streams was around twice that in moorland streams, and lowest of all in streams draining non-native conifers. The unexpected absence of contrasting terrestrial signals in the isotopic data implies that factors other than local land use affect the relative incorporation of allochthonous subsidies into riverine food webs. Nevertheless, our results reveal how planting deciduous riparian trees along temperate headwaters as an adaptation to climate change can modify macroinvertebrate function, increase biomass and potentially enhance resilience by increasing basal resources where cover is extensive (>60 m riparian width). We advocate greater urgency in efforts to understand the ecosystem consequences of climate change adaptation to guide future actions.

Field and laboratory studies reveal interacting effects of stream oxygenation and warming on aquatic ectotherms.

Aquatic ecological responses to climatic warming are complicated by interactions between thermal effects and other environmental stressors such as organic pollution and hypoxia. Laboratory experiments have demonstrated how oxygen limitation can set heat tolerance for some aquatic ectotherms, but only at unrealistic lethal temperatures and without field data to assess whether oxygen shortages might also underlie sublethal warming effects. Here, we test whether oxygen availability affects both lethal and nonlethal impacts of warming on two widespread Eurasian mayflies, Ephemera danica, Müller 1764 and Serratella ignita (Poda 1761). Mayfly nymphs are often a dominant component of the invertebrate assemblage in streams, and play a vital role in aquatic and riparian food webs. In the laboratory, lethal impacts of warming were assessed under three oxygen conditions. In the field, effects of oxygen availability on nonlethal impacts of warming were assessed from mayfly occurrence in 42 293 UK stream samples where water temperature and biochemical oxygen demand were measured. Oxygen limitation affected both lethal and sublethal impacts of warming in each species. Hypoxia lowered lethal limits by 5.5 °C (±2.13) and 8.2 °C (±0.62) for E. danica and S. ignita respectively. Field data confirmed the importance of oxygen limitation in warmer waters; poor oxygenation drastically reduced site occupancy, and reductions were especially pronounced under warm water conditions. Consequently, poor oxygenation lowered optimal stream temperatures for both species. The broad concordance shown here between laboratory results and extensive field data suggests that oxygen limitation not only impairs survival at thermal extremes but also restricts species abundance in the field at temperatures well below upper lethal limits. Stream oxygenation could thus control the vulnerability of aquatic ectotherms to global warming. Improving water oxygenation and reducing pollution can provide key facets of climate change adaptation for running waters.

Anthropogenic modification disrupts species co-occurrence in stream invertebrates.

The question of whether species co-occurrence is random or deterministic has received considerable attention, but little is known about how anthropogenic disturbance mediates the outcomes. By combining experiments, field surveys and analysis against null models, we tested the hypothesis that anthropogenic habitat modification disrupts species co-occurrence in stream invertebrates across spatial scales. Whereas communities in unmodified conditions were structured deterministically with significant species segregation, catchment-scale conversion to agriculture and sediment deposition at the patch- or micro-habitat scale apparently randomized species co-occurrences. This shift from non-random to random was mostly independent of species richness, abundance and spatial scale. Data on community-wide life-history traits (body size, dispersal ability and predatory habits) and beta-diversity indicated that anthropogenic modification disrupted community assembly by affecting biotic interactions and, to a lesser extent, altering habitat heterogeneity. These data illustrate that the balance between predictable and stochastic patterns in communities can reflect anthropogenic modifications that not only transcend scales but also change the relative forces that determine species coexistence. Research into the effects of habitat modification as a key to understanding global change should extend beyond species richness and composition to include species co-occurrence, species interactions and any functional consequences.

Linking interdecadal changes in British river ecosystems to water quality and climate dynamics.

Macroinvertebrate communities in Western European rivers have changed substantially in recent decades. Understanding the causes is challenging because improvements in water quality have coincided with climatic variations over this period. Using data covering >2300 rivers and 21 years (1991-2011) across England and Wales, we analysed family-level distributions and nationwide trends in prevalence (proportion of sampling locations where an organism was present) to diagnose the causes of ecological change. Our aims were to: (i) reveal the taxa driving assemblage-level trends; (ii) identify the main changes in family-level prevalence and distribution patterns; and (iii) test whether changes were accounted for by improving water quality, increasing temperatures or variations in discharge. While previous analyses revealed increasing richness among British river invertebrates, a partial turnover of taxa is now evident. Two distinct components of temporal trend have comprised: (i) overall increases or decreases in taxon prevalence over 21 years, which correlated with pollution sensitivity and discharge; and (ii) short-term variations in prevalence that correlated primarily with temperature and nutrient concentrations. The longer-term changes in prevalence were reflected in expansions or contractions in families' distributions linked to water quality, with little evidence of shifts consistent with increasing temperatures. Although these monitoring data had limitations (e.g., family-level data, few headwaters), they provide no clear evidence of long-term climate effects on invertebrates; the one feature consistent with climate warming - a small northward expansion of the range of many taxa - was accounted for by large improvements in water quality in northern England. Nevertheless, changes linked to discharge and temperature over the shorter-term (<2 years) point to the climatic sensitivity of invertebrate communities. It is therefore likely that any long-term climatic changes since 1990 have been outweighed by the strength and geographical extent of the recovery from poor water quality.

Addressing the challenges of combined sewer overflows.

Europe's ageing wastewater system often combines domestic sewage with surface runoff and industrial wastewaters. To reduce the associated risk of overloading wastewater treatment works during storms, and to prevent wastewater backing-up into properties, Combined Sewer Overflows (CSOs) are designed into wastewater networks to release excess discharge into rivers or coastal waters without treatment. In view of growing regulatory scrutiny and increasing public concern about their excessive discharge frequencies and potential impacts on environments and people, there is a need to better understand these impacts to allow prioritisation of cost-effective solutions.We review: i) the chemical, physical and biological composition of CSOs discharges; ii) spatio-temporal variations in the quantity, quality and load of overflows spilling into receiving waters; iii) the potential impacts on people, ecosystems and economies. Despite investigations illustrating the discharge frequency of CSOs, data on spill composition and loading of pollutants are too few to reach representative conclusions, particularly for emerging contaminants. Studies appraising impacts are also scarce, especially in contexts where there are multiple stressors affecting receiving waters. Given the costs of addressing CSOs problems, but also the likely long-term gains (e.g. economic stimulation as well as improvements to biodiversity, ecosystem services, public health and wellbeing), we highlight here the need to bolster these evidence gaps. We also advocate no-regrets options to alleviate CSO problems taking into consideration economic costs, carbon neutrality, ecosystem benefit and community well-being. Besides pragmatic, risk-based investment by utilities and local authorities to modernise wastewater systems, these include i) more systemic thinking, linking policy makers, consumers, utilities and regulators, to shift from local CSO issues to integrated catchment solutions with the aim of reducing contributions to wastewater from surface drainage and water consumption; ii) broader societal responsibilities for CSOs, for example through improved regulation, behavioural changes in water consumption and disposal of waste into wastewater networks, and iii) greater cost-sharing of wastewater use.

Potential drivers of changing ecological conditions in English and Welsh rivers since 1990.

River invertebrate communities across Europe have been changing in response to variations in water quality over recent decades, but the underlying drivers are difficult to identify because of the complex stressors and environmental heterogeneity involved. Here, using data from ∼4000 locations across England and Wales, collected over 29 years, we use three approaches to help resolve the drivers of spatiotemporal variation in the face of this complexity: i) mapping changes in invertebrate richness and community composition; ii) structural equation modelling (SEM) to distinguish land cover, water quality and climatic influences; and iii) geographically weighted regression (GWR) to identify how the apparent relationships between invertebrate communities and abiotic variables change across the area. Mapping confirmed widespread increases in richness and the proportion of pollution-sensitive taxa across much of England and Wales. It also revealed regions where pollution-sensitive taxa or overall richness declined, the former primarily in the uplands. SEMs confirmed strong increases in average biochemical oxygen demand and nutrient concentrations related to urban and agricultural land cover, but only a minority of land cover's effect upon invertebrate communities was explained by average water chemistry, highlighting potential factors such as episodic extremes or emerging contaminants. GWR identified strong geographical variation in estimated relationships between macroinvertebrate communities and environmental variables, with evidence that the estimated negative impacts of nutrients and water temperature were increasing through time. Overall the results are consistent with widespread biological recovery of Britain's rivers from past gross organic pollution, whilst highlighting declines in some of the most diverse and least impacted streams. Modelling points to a complex and changing set of drivers, highlighting the multifaceted impacts of catchment land cover and the evolving role of different stressors, with the relationship to gross organic pollution weakening, whilst estimated nutrient and warming effects strengthened.

Eurasian dipper eggs indicate elevated organohalogenated contaminants in urban rivers.

Many urban European streams are recovering from industrial, mining, and sewage pollution during the 20th century. However, associated recolonization by clean water organisms can potentially result in exposure to legacy or novel toxic pollutants that persist in the environment. Between 2008 and 2010, we sampled eggs of a river passerine, the Eurasian dipper (Cinclus cinclus), from 33 rivers in South Wales and the English borders (UK) which varied in catchment land use from rural to highly urbanized. Dipper egg δ(15)N and δ(13)C stable isotopes were enriched from urban rivers while δ(34)S was strongly depleted, effectively discriminating their urban or rural origins at thresholds of 10% urban land cover or 1000 people/km(2). Concentrations of total polychlorinated biphenyls (PCBs) and polybrominated biphenyl ethers (PBDEs) were positively related to urban land cover and human population density while legacy organochlorine pesticides such as p,p'-DDE, lindane, and hexachlorobenzene were found in higher concentrations at rural sites. Levels of PBDEs in urban dipper eggs (range of 136-9299 ng/g lw) were among the highest ever reported in passerines, and some egg contaminants were at or approaching levels sufficient for adverse effects on avian development. With the exception of dieldrin, our data shows PCBs and other organochlorine pesticides have remained stable or increased in the past 20 years in dipper eggs, despite discontinued use.

Evidence of biological recovery from gross pollution in English and Welsh rivers over three decades.

Uncertainty around the changing ecological status of European rivers reflects an evolving array of anthropogenic stressors, including climate change. Although previous studies have revealed some recovery from historical pollution in the 1990s and early-2000s, there are contrasting trends among pollutants across Europe and recovery may have stalled or been reversed. To provide more contemporary evidence on trends and status, here we investigate changes in English and Welsh river macroinvertebrate communities over almost 30 years (1991-2019) using a network of nearly 4000 survey locations. Analysis comprised: i) trends in taxonomic and functional richness, community composition and ecological traits, ii) gains, losses and turnover of taxa, and the overall homogeneity of macroinvertebrate communities nationally, and iii) an exploration of how temporal trends varied with catchment characteristics. Taxonomic richness increased, primarily in the 1990s, whilst a shift towards pollution-sensitive taxa continued throughout the study period, accompanied by a growing prevalence in traits such as preferences for fast-flowing conditions, coarser substrata, and 'shredding' or 'scraping' feeding strategies. Changes consistent with improvement occurred in both urbanised and agricultural catchments, but were more pronounced in urban rivers as they gained pollution sensitive taxa that were otherwise more prevalent in rural rivers. Overall, these results indicate continuing biological recovery from organic pollution, consistent with national scale trends in water quality. Results reemphasise the importance of looking at multiple facets of diversity, with periods of near-constant richness disguising changes in taxonomic and functional composition. Whilst this national-scale picture is broadly positive, we highlight the need to investigate more local variations or pollutants that depart from this aggregate picture.

The occurrence and zoonotic potential of Cryptosporidium species in freshwater biota.

BACKGROUND: Protozoan pathogens from the genus Cryptosporidium cause the diarrhoeal disease cryptosporidiosis in humans and animals globally. Freshwater biota could act as potential reservoirs or zoonotic sources of Cryptosporidium infections for livestock and people, but Cryptosporidium occurrence in aquatic biota is largely unexplored. The aim of this study was to investigate the occurrence of Cryptosporidium in a range of freshwater organisms in upland rivers across England and Wales. METHODS: Fish were sampled by electrofishing, invertebrate larvae by kick sampling and the otter Lutra lutra and mink Mustela vison through faecal samples collected opportunistically as part of a nation-wide study. PCR targeting the small subunit ribosomal RNA gene was used to detect Cryptosporidium species. RESULTS: Cryptosporidium occurred in just 0.8% of all the samples and in none of 73 samples from nine invertebrate genera. Cryptosporidium was detected in two of 2/74 fish samples (2.7%), both salmonids, and in 2/92 otter faecal samples (2.17%), but there were no positive samples in mink (0/24) or the bullhead Cottus gobio (0/16). CONCLUSIONS: Low detection rate of human-infective Cryptosporidium species in aquatic fauna indicates they may present a low risk of contamination of some upland freshwaters.

American dippers indicate contaminant biotransport by Pacific salmon.

Migrating salmon can increase productivity in Pacific Northwestern streams and lakes through the deposition of nutrients from their decomposing carcasses after spawning. Several studies also report simultaneous biotransport of persistent organic pollutants that have contaminated lake food webs, although no similar effect has been shown conclusively in rivers. We tested the prediction that salmon enhance contaminants in river food webs using the American dipper (Cinclus mexicanus), an aquatic songbird and a recognized indicator of stream quality. Over 3 years, we analyzed 29 dipper eggs and aquatic invertebrate samples from 14 different rivers in 10 catchments in southern British Columbia, Canada to assess whether variations in autumn spawning density of Pacific salmon were reflected in dipper egg contamination or stable carbon and nitrogen isotopes. δ(13)C isotope signatures, but not δ(15)N, in aquatic invertebrates and dipper eggs increased among catchments in proportion to the average density of spawning salmon. Concentrations of brominated flame retardants (PBDEs), dichlorodiphenyltrichloroethane metabolites (DDTs), and chlordane compounds were related in part to the δ(13)C measure of salmon density, but mercury, chlorobenzenes, and polychlorinated biphenyls (PCBs) were explained better by dipper trophic level. We conclude that spawning Pacific salmon result in the increased availability of salmon fry as dipper prey and salmon are a significant source of PBDEs, DDTs, and chlordanes to river ecosystems. However, contrary to lake studies, postspawn concentrations of legacy PCBs in river birds, even in salmon-rich rivers, were not significantly higher than would be expected from atmospheric deposition alone. We recommend using δ(13)C isotopes to trace salmon-derived lipids which may persist over winter particularly in rivers, and are potentially a better reflection of lipophilic contaminant transfer.

Community assembly, functional traits, and phylogeny in Himalayan river birds.

Heterogeneity in riverine habitats acts as a template for species evolution that influences river communities at different spatio-temporal scales. Although birds are conspicuous elements of these communities, the roles of phylogeny, functional traits, and habitat character in their niche use or species' assembly have seldom been investigated. We explored these themes by surveying multiple headwaters over 3000 m of elevation in the Himalayan Mountains of India where the specialist birds of montane rivers reach their greatest diversity on Earth. After ordinating community composition, species traits, and habitat character, we investigated whether river bird traits varied with elevation in ways that were constrained or independent of phylogeny, hypothesizing that trait patterns reflect environmental filtering. Community composition and trait representation varied strongly with increasing elevation and river naturalness as species that foraged in the river/riparian ecotone gave way to small insectivores with direct trophic dependence on the river or its immediate channel. These trends were influenced strongly by phylogeny as communities became more clustered by functional traits at a higher elevation. Phylogenetic signals varied among traits, however, and were reflected in body mass, bill size, and tarsus length more than in body size, tail length, and breeding strategy. These variations imply that community assembly in high-altitude river birds reflects a blend of phylogenetic constraint and habitat filtering coupled with some proximate niche-based moulding of trait character. We suggest that the regional co-existence of river birds in the Himalaya is facilitated by this same array of factors that together reflect the highly heterogeneous template of river habitats provided by these mountain headwaters.

Using diatoms as quality indicators for a newly-formed urban lake and its catchment.

Periphytic diatoms were studied to evaluate the water quality of a newly created lake, formed by the enclosure of the formerly tidal Cardiff Bay (Wales, UK), and the effects of two inflowing rivers which drain densely populated and industrialised catchments. Seven sites in Cardiff Bay and two locations on the inflowing rivers were monitored for diatoms and water chemistry over 2 years. Water quality was assessed using a revised UK trophic diatom index (TDI) and new methods to determine ecological quality ratios and ecological status classes as required by the EU Water Framework Directive. Diatom assemblages reflected spatiotemporal variations in environmental conditions between the rivers and Cardiff Bay and within the bay. In the bay, diatoms reflected differences in river quality and possibly local pollution in certain areas of the lake. High values of the TDI indicated eutrophic to hypertrophic conditions in both rivers and in the bay and diatoms indicated poor ecological status.

Estimating the size distribution of plastics ingested by animals.

The ingestion of plastics appears to be widespread throughout the animal kingdom with risks to individuals, ecosystems and human health. Despite growing information on the location, abundance and size distribution of plastics in the environment, it cannot be assumed that any given animal will ingest all sizes of plastic encountered. Here, we use published data to develop an allometric relationship between plastic consumption and animal size to estimate the size distribution of plastics feasibly ingested by animals. Based on more than 2000 gut content analyses from animals ranging over three orders of magnitude in size (lengths 9 mm to 10 m), body length alone accounts for 42% of the variance in the length of plastic an animal may ingest and indicates a size ratio of roughly 20:1 between animal body length and the largest plastic the animal may ingest. We expect this work to improve global assessments of plastic pollution risk by introducing a quantifiable link between animals and the plastics they can ingest.

Environment and food web structure interact to alter the trophic magnification of persistent chemicals across river ecosystems.

Legacy organic pollutants persist in freshwater environments, but there is limited understanding of how their trophic transfer and effects vary across riverine ecosystems with different land use, biological communities and food webs. Here, we investigated the trophic magnification of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and a suite of organochlorines (OCs) across nine riverine food webs in contrasting hydrological catchments across South Wales (United Kingdom). Pollutants biomagnified through the food webs in all catchments studied, in some cases reaching levels sufficient for biological effects on invertebrates, fish and river birds such as the Dipper (Cinclus cinclus). Trophic magnification differed across food webs depending on pollutant characteristics (e.g. octanol-water partitioning coefficient) and site-specific environmental conditions (e.g. land use, water chemistry and basal resource composition). The trophic magnification of PBDEs, PCBs and OCs also reflected food-web structure, with greater accumulation in more connected food webs with more generalist taxa. These data highlight interactions between pollutant properties, environmental conditions and biological network structure in the transfer and biomagnification of POPs in river ecosystems. We advocate the need for further investigations of system-specific transfers of contaminants through aquatic food webs as these factors appear to have important implications for risk assessment.

Impacts of multiple stressors on freshwater biota across spatial scales and ecosystems.

Climate and land-use change drive a suite of stressors that shape ecosystems and interact to yield complex ecological responses (that is, additive, antagonistic and synergistic effects). We know little about the spatial scales relevant for the outcomes of such interactions and little about effect sizes. These knowledge gaps need to be filled to underpin future land management decisions or climate mitigation interventions for protecting and restoring freshwater ecosystems. This study combines data across scales from 33 mesocosm experiments with those from 14 river basins and 22 cross-basin studies in Europe, producing 174 combinations of paired-stressor effects on a biological response variable. Generalized linear models showed that only one of the two stressors had a significant effect in 39% of the analysed cases, 28% of the paired-stressor combinations resulted in additive effects and 33% resulted in interactive (antagonistic, synergistic, opposing or reversal) effects. For lakes, the frequencies of additive and interactive effects were similar for all spatial scales addressed, while for rivers these frequencies increased with scale. Nutrient enrichment was the overriding stressor for lakes, with effects generally exceeding those of secondary stressors. For rivers, the effects of nutrient enrichment were dependent on the specific stressor combination and biological response variable. These results vindicate the traditional focus of lake restoration and management on nutrient stress, while highlighting that river management requires more bespoke management solutions.

Microplastic ingestion by riverine macroinvertebrates.

Although microplastics are a recognised pollutant in marine environments, less attention has been directed towards freshwater ecosystems despite their greater proximity to possible plastic sources. Here, we quantify the presence of microplastic particles (MPs) in river organisms upstream and downstream of five UK Wastewater Treatment Works (WwTWs). MPs were identified in approximately 50% of macroinvertebrate samples collected (Baetidae, Heptageniidae and Hydropsychidae) at concentrations up to 0.14 MP mg tissue-1 and they occurred at all sites. MP abundance was associated with macroinvertebrate biomass and taxonomic family, but MPs occurred independently of feeding guild and biological traits such as habitat affinity and ecological niche. There was no increase in plastic ingestion downstream of WwTW discharges averaged across sites, but MP abundance in macroinvertebrates marginally increased where effluent discharges contributed more to total runoff and declined with increasing river discharge. The ubiquity of microplastics within macroinvertebrates in this case study reveals a potential risk from MPs entering riverine food webs through at least two pathways, involving detritivory and filter-feeding, and we recommend closer attention to freshwater ecosystems in future research.