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We present combined theoretical and experimental work investigating the angle-resolved phases of the photoionization process driven by a two-color field consisting of an attosecond pulse train and an infrared pulse in an ensemble of randomly oriented molecules. We derive a general form for the two-color photoelectron (and time-delay) angular distribution valid also in the case of chiral molecules and when relative polarizations of the photons contributing to the attosecond photoelectron interferometer differ. We show a comparison between the experimental data and theoretical predictions in an ensemble of methane and deuteromethane molecules, discussing the effect of nuclear dynamics on the photoionization phases. Finally, we demonstrate that the oscillating component and the phase of the two-color signal can be fitted by using complex asymmetry parameters, in perfect analogy to the atomic case.
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We have built and characterized, to our knowledge, the first six-telescope discrete beam combiner (DBC) for stellar interferometry in the astronomical J-band. It is the DBC with the largest number of beam combinations and was manufactured using ultrafast laser inscription in borosilicate glass, with a throughput of ≈56%. For calibration of the visibility-to-pixel matrix, we use a two-input Michelson interferometer and extract the complex visibility. A visibility amplitude of 1.05 and relative precision of 2.9% and 3.8% are extracted for 1328 nm and 1380 nm, respectively. Broadband (≤40n m) characterization is affected by dispersion but shows similar performance.
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We report the fabrication of alkali-metal vapor cells using femtosecond laser machining. This laser-written vapor-cell (LWVC) technology allows arbitrarily-shaped 3D interior volumes and has potential for integration with photonic structures and optical components. We use non-evaporable getters both to dispense rubidium and to absorb buffer gas. This enables us to produce cells with sub-atmospheric buffer gas pressures without vacuum apparatus. We demonstrate sub-Doppler saturated absorption spectroscopy and single beam optical magnetometry with a single LWVC. The LWVC technology may find application in miniaturized atomic quantum sensors and frequency references.
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Structured Illumination Microscopy (SIM) is a key technology for high resolution and super-resolution imaging of biological cells and molecules. The spread of portable and easy-to-align SIM systems requires the development of novel methods to generate a light pattern and to shift it across the field of view of the microscope. Here we show a miniaturized chip that incorporates optical waveguides, splitters, and phase shifters, to generate a 2D structured illumination pattern suitable for SIM microscopy. The chip creates three point-sources, coherent and controlled in phase, without the need for further alignment. Placed in the pupil of a microscope's objective, the three sources generate a hexagonal illumination pattern on the sample, which is spatially translated thanks to thermal phase shifters. We validate and use the chip, upgrading a commercial inverted fluorescence microscope to a SIM setup and we image biological sample slides, extending the resolution of the microscope.
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Iluminação , Dispositivos Ópticos , Microscopia de Fluorescência/métodosRESUMO
In this Letter, we propose a fabrication technique based on femtosecond laser secondary direct writing (FsLSDW) that allows us to statically reset the beam-splitting ratio of directional couplers. By modifying the interaction region with a second inscription, the coupling coefficient of the reconstructed devices can be indeed changed continuously within the range of 0.49-2.1 rad/mm, thus enabling a complete tunability of the reconstructed splitting ratio from zero to full power transfer between the waveguides. This powerful reconstruction capability facilitates the arbitrary reset of an imperfect device, from any initial splitting ratio to the correct one. In the future, such static control method could potentially solve the fabrication error problem in the manufacturing of high-fidelity large-scale integrated photonic quantum chips.
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Yield stress materials deform as elastic solids or flow as viscous liquids, depending on the applied stress, which also allows them to trap particles below a certain size or density threshold. To investigate the conditions for such a transition at the microscale, we use an optofluidic microrheometer, based on the scattering of an infrared beam onto a microbead, which reaches forces in the nN scale. We perform creep experiments on a model soft material composed of swollen microgels, determining the limits of linear response and yield stress values, and observe quantitative agreement with bulk measurements. However, the motion of the microbead, both below and above yielding, reflects distinctive microscale features of the surrounding material, whose plastic rearrangements were investigated by us using small, passive tracers.
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We present the first on-sky results of a four-telescope integrated optics discrete beam combiner (DBC) tested at the 4.2 m William Herschel Telescope. The device consists of a four-input pupil remapper followed by a DBC and a 23-output reformatter. The whole device was written monolithically in a single alumino-borosilicate substrate using ultrafast laser inscription. The device was operated at astronomical H-band (1.6 µm), and a deformable mirror along with a microlens array was used to inject stellar photons into the device. We report the measured visibility amplitudes and closure phases obtained on Vega and Altair that are retrieved using the calibrated transfer matrix of the device. While the coherence function can be reconstructed, the on-sky results show significant dispersion from the expected values. Based on the analysis of comparable simulations, we find that such dispersion is largely caused by the limited signal-to-noise ratio of our observations. This constitutes a first step toward an improved validation of the DBC as a possible beam combination scheme for long-baseline interferometry.
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In this work we demonstrate novel integrated-optics modulators and switches, realized in a glass substrate by femtosecond laser pulses. These devices are based on oscillating microcantilevers, machined by water-assisted laser ablation. Single-mode optical waveguides are laser-inscribed inside the cantilever beam and continue in the substrate beyond the cantilever's tip. By exciting the resonant oscillation of the mechanical structure, coupling between the waveguide segments is varied in time. Operation frequencies are in the range of tens of kilohertz, thus they markedly overcome the response-time limitation of other glass-based modulators, which rely on the thermo-optic effect. These components may be integrated in more complex waveguide circuits or optofluidic lab-on-chips, to provide periodic and high-frequency modulation of the optical signals.
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Three-dimensional culture systems and suitable substrates topographies demonstrated to drive stem cell fate in vitro by mechanical conditioning. For example, the Nichoid 3D scaffold remodels stem cells and shapes nuclei, thus promoting stem cell expansion and stemness maintenance. However, the mechanisms involved in force transmission and in biochemical signaling at the basis of fate determination are not yet clear. Among the available investigation systems, confocal fluorescence microscopy using fluorescent dyes enables the observation of cell function and shape at the subcellular scale in vital and fixed conditions. Contrarily, nonlinear optical microscopy techniques, which exploit multi-photon processes, allow to study cell behavior in vital and unlabeled conditions. We apply confocal fluorescence microscopy, coherent anti-Stokes Raman scattering (CARS), and second harmonic generation (SHG) microscopy to characterize the phenotypic expression of mesenchymal stem cells (MSCs) towards adipogenic and chondrogenic differentiation inside Nichoid scaffolds, in terms of nuclear morphology and specific phenotypic products, by comparing these techniques. We demonstrate that the Nichoid maintains a rounded nuclei during expansion and differentiation, promoting MSCs adipogenic differentiation while inhibiting chondrogenesis. We show that CARS and SHG techniques are suitable for specific estimation of the lipid and collagenous content, thus overcoming the limitations of using unspecific fluorescent probes.
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Diferenciação Celular/fisiologia , Células-Tronco Mesenquimais/fisiologia , Alicerces Teciduais/química , Adipogenia/fisiologia , Animais , Células Cultivadas , Condrogênese/fisiologia , Corantes Fluorescentes/metabolismo , Células-Tronco Mesenquimais/metabolismo , Microscopia Confocal/métodos , Ratos , Análise Espectral Raman/métodosRESUMO
We demonstrate the generation of few-cycle deep ultraviolet pulses via frequency upconversion of 5-fs near-infrared pulses in argon using a laser-fabricated gas cell. The measured spectrum extends from 210 to 340 nm, corresponding to a transform-limited pulse duration of 1.45 fs. We extract from a dispersion-free second-order cross-correlation measurement a pulse duration of 1.9 fs, defining a new record in the deep ultraviolet spectral range.
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The decay of an unstable system is usually described by an exponential law. Quantum mechanics predicts strong deviations of the survival probability from the exponential: Indeed, the decay is initially quadratic, while at very large times it follows a power law, with superimposed oscillations. The latter regime is particularly elusive and difficult to observe. Here we employ arrays of single-mode optical waveguides, fabricated by femtosecond laser direct inscription, to implement quantum systems where a discrete state is coupled and can decay into a continuum. The optical modes correspond to distinct quantum states of the photon, and the temporal evolution of the quantum system is mapped into the spatial propagation coordinate. By injecting coherent light states in the fabricated photonic structures and by measuring a small scattered fraction of such light with an unprecedented dynamic range, we are able to experimentally observe not only the exponential decay regime, but also the quadratic Zeno region and the power-law decay at long evolution times.
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We report on the quantum storage of a heralded frequency-multiplexed single photon in an integrated laser-written rare-earth doped waveguide. The single photon contains 15 discrete frequency modes separated by 261 MHz and spanning across 4 GHz. It is obtained from a nondegenerate photon pair created via cavity-enhanced spontaneous down-conversion, where the heralding photon is at telecom wavelength and the heralded photon is at 606 nm. The frequency-multimode photon is stored in a praseodymium-doped waveguide using the atomic frequency comb (AFC) scheme, by creating multiple combs within the inhomogeneous broadening of the crystal. Thanks to the intrinsic temporal multimodality of the AFC scheme, each spectral bin includes 9 temporal modes, such that the total number of stored modes is about 130. We demonstrate that the storage preserves the nonclassical properties of the single photon, and its normalized frequency spectrum.
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Last decade's advancements in optofluidics allowed obtaining an ever increasing integration of different functionalities in lab on chip devices to culture, analyze, and manipulate single cells and entire biological specimens. Despite the importance of optical imaging for biological sample monitoring in microfluidics, imaging is traditionally achieved by placing microfluidics channels in standard bench-top optical microscopes. Recently, the development of either integrated optical elements or lensless imaging methods allowed optical imaging techniques to be implemented in lab on chip systems, thus increasing their automation, compactness, and portability. In this review, we discuss known solutions to implement microscopes on chip that exploit different optical methods such as bright-field, phase contrast, holographic, and fluorescence microscopy.
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Técnicas Analíticas Microfluídicas/métodos , Microfluídica/instrumentação , Microfluídica/métodos , Microscopia/instrumentação , Microscopia/métodos , Imagem Óptica/instrumentação , Imagem Óptica/métodos , Automação/instrumentação , Automação/métodos , Holografia/instrumentação , Holografia/métodos , Humanos , Dispositivos Lab-On-A-Chip , Técnicas Analíticas Microfluídicas/instrumentaçãoRESUMO
We study analytically the polarization behaviour of directional couplers composed of birefringent waveguides, showing that they can induce polarization transformations that depend on the specific input-output path considered. On the basis of this study, we propose and demonstrate experimentally, by femtosecond laser writing, directional couplers that yield a polarization-independent power splitting and, at the same time, preserve the polarization state of the propagating light. More in detail, we devise two different approaches to realize such devices: the first one is based on local birefringence engineering by additional refractive index modification tracks, while the second one exploits ultra-low birefringence waveguides (b = 1.2 × 10-6), obtained by thermal annealing.
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The viscosity of gel-forming fluids is notoriously complex and its study can benefit from new model systems that enable a detailed control of the network features. Here we use a novel and simple microfluidic-based active microrheology approach to study the transition from Newtonian to non-Newtonian behavior in a DNA hydrogel whose structure, connectivity, density of bonds, bond energy and kinetics are strongly temperature dependent and well known. In a temperature range of 15 °C, the system reversibly and continuously transforms from a Newtonian dispersion of low-valence nanocolloids into a strongly shear-thinning fluid, passing through a set of intermediate states where it behaves as a power-law fluid. We demonstrate that the knowledge of network topology and bond free energy enables to quantitatively predict the observed behavior using established rheology models.
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Microfluidic lenses are relevant optical components for sensing application in lab-on-a-chip devices, guaranteeing a robust alignment of the elements, a high level of compactness and tunable optical properties. In this work we describe an innovative integrated in-plane microfluidic lens. The device shows both an optimized shape capable of reducing spherical aberrations and periodically tunable optical properties. Indeed through the combination of the lens with a droplet generator module, we have been able to obtain an integrated optofluidic modulator capable of both on-demand on/off switching and periodic modulation of light. The device possesses a simple 3D geometry, which has been realized by exploiting the 3D capability of the femtosecond laser micromachining fabrication technique.
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Atomic force microscopy (AFM) nanoindentation of soft materials is a powerful tool for probing mechanical properties of biomaterials. Though many results have been reported in this field over the last decade, adhesion forces between the tip and the sample hinder the elastic modulus measurement when hydrophilic soft samples are investigated. Here, two-photon polymerization (2PP) technology was used to fabricate hydrophobic perfluoropolyether-based AFM tips. The hydrophobic 2PP tips allowed us to overcome the limitations of commercial and functionalized tips as well as to successfully measure the elastic modulus of medically relevant soft materials in air. Our results obtained in the characterization of poly(dimethyl siloxane) and polyethylene glycol hydrogels showed lower adhesion forces over a larger measurement range when compared to measurements performed with commercial tips. The elastic moduli measured by means of hydrophobic 2PP AFM tips were also found to be comparable to those obtained using conventional techniques for macroscopic samples. We successfully showed that the hydrophobic AFM tips developed by this highly versatile technology enable the study of mechanical properties of soft matter, benefiting from reduced sample-tip interactions, and a custom-made shape and dimension of the tips.
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Developing versatile joining techniques to weld transparent materials on a micrometer scale is of great importance in a growing number of applications, especially for the fabrication and assembly of biomedical devices. In this paper, we report on fs-laser microwelding of two transparent layers of polymethyl methacrylate (PMMA) based on nonlinear absorption and localized heat accumulation at high repetition rates. A fiber CPA laser system was used delivering 650-fs pulses at 1030 nm with repetition rates in the MHz regime. The laser-induced modifications produced by the focused beam into the bulk PMMA were firstly investigated, trying to find a suitable set of process parameters generating continuous and localized melting. Results have been evaluated based on existing heat accumulation models. Then, we have successfully laser welded two 1-mm-thick PMMA layers in a lap-joint configuration. Sealing of the sample was demonstrated through static and dynamic leakage tests. This fs-laser micro-welding process does not need any pre-processing of the samples or any intermediate absorbing layer. Furthermore, it offers several advantages compared to other joining techniques, because it prevents contamination and thermal distortion of the samples, thus being extremely interesting for application in direct laser fabrication of microfluidic devices.
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Quantum mechanical decay, Fano interference, and bound states with energy in the continuum are ubiquitous phenomena in different areas of physics. Here we experimentally demonstrate that particle statistics strongly affects quantum mechanical decay in a multiparticle system. By considering propagation of two-photon states in engineered photonic lattices, we simulate quantum decay of two noninteracting particles in a multilevel Fano-Anderson model. Remarkably, when the system sustains a bound state in the continuum, fractional decay is observed for bosonic particles, but not for fermionic ones. Complete decay in the fermionic case arises because of the Pauli exclusion principle, which forbids the bound state to be occupied by the two fermions. Our experiment indicates that particle statistics can tune many-body quantum decay from fractional to complete.