Diagnosis of Vitality in Skin Wounds in the Ligature Marks Resulting From Suicide Hanging.

Ascertaining the vital origin of skin wounds is one of the most challenging problems in forensic pathology. The forensic literature describes biomarkers and methods for differentiating vital and postmortem wounds, although no clear conclusions have been reached. The aim of this study was to characterize human vital wounds by analyzing the concentrations of metallic ions and the expression of P-selectin and cathepsin D in skin wounds in the ligature marks in a cohort of suicidal hangings for which vitality was previously demonstrated.A total of 71 skin wounds were analyzed within a postmortem interval of 19 to 36 hours. The concentration of Fe, Zn, Mg, and Ca and the expression of P-selectin and cathepsin D were analyzed together and separately. The majority of autopsied suicidal hangings were men (86%) with complete hanging mode (60.7%) in which there was a high frequency of subcutaneous injuries (78.3%). High concentrations of Ca and Mg compared with Fe and Zn were found. Ca and Zn concentrations decreased, and Fe concentration increased with the seriousness of the injury. A high percentage of moderately negative expression of both proteins was correlated with subcutaneous injury and low or medium concentrations of Fe.In conclusion, the joint study of metallic ions and proteins allows to characterize and to differentiate an injured vital wound of noninjured skin, especially when the damage in the tissue affects to the majority of the structures of the skin, but these results will need to be complemented with other biomarkers in time-controlled samples to further help in the differentiation of vital and postmortem wounds.

Drug-facilitated sexual assault and other crimes: A systematic review by countries.

Drug-facilitated sexual assault (DFSA) and drug-facilitated crime (DFC) constitute a mode of violence that is generally unknown to the population and may go unnoticed by health professionals. The aim of this systematic review was to analyze the victims of DFC, compiling their sociodemographic characteristics, the toxic substances used and their biological matrices and modes of action, in order to identify the substances that are commonly put to criminal use. The aim would be to establish political and health strategies that inform and warn people about possible criminal social behaviors consequent danger to health. This systematic review was conducted following the PRISMA guidelines. Alcohol, benzodiazepines and cocaine were among the most commonly detected substances. In most of the hospitals, immunoassays, liquid chromatography (LC-MS), or gas chromatography-mass spectrometry (GC-MS) analyses were used to identify the substances, while the most frequently used biological matrices were blood and urine. From a judicial point of view, the instrumental protocols and techniques followed for the detection of toxics in different biological matrices must guarantee the reliability and validity of the results for use in a court of law. The recommendations of international organizations should be followed and must be called upon to strengthen their respective national laws against this chemical submission (CS) phenomenon.

Ethical problems related to information and pharmaceutical care in Spain.

This paper represents a reflection on the limits and objectives of the information pharmacists should offer in pharmacies. The obligation of a pharmacist to follow the patient's therapeutic progress makes it necessary to integrate this figure into an ethical-legal framework and to define the objective of the health-related information offered, taking into account the patient's welfare and constitutional rights.

The use and usefulness of information for patients undergoing anaesthesia.

Informed consent is a process rather than just simply the signing of a form. It should provide the patient with the information and understanding needed to authorize a procedure. The aim of the present study was to try to understand the patient's attitude to, and understanding of, the pre-anaesthesia informed consent (IC) document. A prospective descriptive study was made of 159 adults who attended a pre-operative consultation prior to programmed surgery at a teaching hospital. The patients were given a questionnaire on different aspects of the pre-anaesthesia IC document they had signed (ease of understanding and other aspects of the information received and expected) and five questions on how well they remembered the information given in the IC form they had read previously. A series of epidemiological variables, anaesthesia and surgery-associated risks (ASA) and whether the patients had undergone anaesthesia previously, were evaluated. The mean age of the study population was 55 (SD:19.63), with 36% over the age of 65, while 77% had not received secondary education. 21% of the patients who signed did not read the document and 14% found it difficult to understand. 89% found the information sufficient and 46% said they preferred to receive such information in written form. 64% had no or very little recall of the information they had been given, the proportion of those making this claim being in direct relation with age over 50 years and low level of studies. The findings suggest that improvements are needed in the pre-anaesthesia informed consent document so that, rather than serving as a mere legal prerequisite, it fulfils its purpose of providing the patient with the information necessary and in a clearly understood way so that he/she can authorize the proposed surgery.

Parasitism and Optimization of Hyposoter didymator (Hymenoptera: Ichneumonidae) Rearing on Spodoptera littoralis and Helicoverpa armigera (Lepidoptera: Noctuidae).

This study was carried out to improve the rearing methods of endoparasite Hyposoter didymator (Thunberg) for biological control release. The growth and the development of H. didymator on the first, second, and third instars of Spodoptera littoralis (Boisduval) and Helicoverpa armigera (Hübner) under laboratory conditions were investigated. Results indicated that S. littoralis was a suitable host for the rearing of H. didymator . The development time of the parasite on the second instar of both hosts was almost the same (∼17 d). The age of the female parasite affected the percentage of adult emergence, pupal mortality, and females in the offspring reared on S. littoralis host. The best copulation period of H. didymator females and males was 36 h that resulted in 51.8% of females in the offspring. The male to female ratio during copulation period, as well as the presence of males after copulation, and the number of host larvae for each parasite female had no effect on parasitism. Results suggested that S. littoralis second instar is a suitable host for mass rearing of H. didymator parasite, which may improve the use of H. didymator as a biocontrol agent.

Biochemical analysis and immunohistochemical determination of cardiac troponin for the postmortem diagnosis of myocardial damage.

Cardiac disease is the most common cause of sudden death in Western nations. In forensic practice there is a need for more sensitive diagnostic methods for the postmortem diagnosis of myocardial damage. The aim of this study was to analyse the diagnostic efficacy of biochemical markers in cadaver fluids in conjunction with histological studies and the immunohistochemical determination of cardiac troponin C (cTnC) and cardiac troponin T (cTnT) levels in myocardial tissue fixed in formol and included in paraffin. We studied 50 cadavers (43 males and 7 females) with a mean age of 47.5 years (SD 19.2; range 12 to 87 years). Cases were chosen according to the postmortem interval, cause of death, and circumstances of death. Pericardial fluid and serum were tested in duplicate for cardiac troponin I (cTn I), myoglobin and CKMB by immunoassay system using commercial kits. In myocardial tissue, histological studies were performed with hematoxylin and eosin (HE), Masson's trichrome staining and immunohistochemical techniques involving streptavidin-biotin-peroxidase were performed. The results pointed to statistically significant differences for all the biochemical markers in pericardial fluid. The highest levels were obtained in the group of cadavers who had died from myocardial infarction. The immunohistochemical expression of cTnC was detected in 86% of cases; it was strongly positive and usually diffuse. The expression of cTnT, was much less frequent (46% of cases) and less intense. It was concluded that the immunohistochemical determination of cTnC and cTnT levels in myocardial tissue may be used as an index of myocardium damage.

Biochemical assessment of acute myocardial ischaemia.

AIMS: To evaluate the efficacy of biochemical parameters in different fluids in the diagnosis of myocardial infarction of different causes, analysed after death. METHODS: The myoglobin concentration and total creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) activities were measured in serum, pericardial fluid, and vitreous humour from seven diagnostic groups of cadavers classified according to the severity of myocardial ischaemia and cause of death. Lactate dehydrogenase (LDH) and myosin were measured only in serum and pericardial fluid, and cathepsin D only in pericardial fluid. Routine haematoxylin and eosin and acridine orange staining were used for microscopy studies of heart tissue. RESULTS: In pericardial fluid there were substantial differences between the different groups with respect to CK, CK-MB, and LDH activities and myosin concentrations. The highest values were found in cases with morphological evidence of myocardial ischaemia. CONCLUSIONS: Biochemical parameters, which reach the pericardial fluid via passive diffusion and ultrafiltration due to a pressure gradient, were thus detectable in this fluid earlier than in serum in cases with myocardial ischaemia. These biochemical parameters may be of use for ruling out myocardial ischaemia in those controversial cases in which reliable morphological findings are lacking.

HCH and DDT residues in human placentas in Murcia (Spain).

Organochlorinated insecticides are ubiquitous toxicants that are transplacentally transferred from mother to fetus and are reported to produce adverse health effects in pregnant woman and neonates. To investigate hexachlorocyclohexane (HCH) and 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) exposure in pregnant woman, a total of 102 placentas were analyzed by a gas chromatograph equipped with electron capture detector. Organochlorine residues were found in 69.6% of the samples. Lindane was detected in 24.5%, alpha-HCH in 2.9%, beta-HCH in 6.9%, p,p'-DDE in 44.1%, p,p'-DDT in 14.7% and p,p'-DDD in 10.8% of the samples. The pattern of dispersal by these substances in Murcia is similar to that described by different authors in other countries. Therefore, the past use of DDT and the present employment of lindane seem to be reflected in body tissues of the residents of this area.

Diagnosis of pregnancy in bloodstains. Study of progesterone and luteinizing hormone levels.

The objective of this work is to establish the diagnostic efficiency of the determination of progesterone and luteinizing hormone in bloodstains on an absorbent surface. In a stain consisting of woman's blood, the establishment of pregnancy or birth may be significant in cases of criminal abortion or infanticide, or to determine the probable date of the events. A total of 61 women between the ages of 16 and 47 years were studied (mean age 29.54 years; S.D. 7.82). Subjects were distributed in three groups: pregnant women, women in labour and non-pregnant women. We found statistically significant differences in progesterone and LH levels between the non-pregnant group and pregnant women in labour in 24 and 48-h bloodstains. We found no statistically significant differences between progesterone and LH levels in pregnant women in 24, 48 and 72-h stains and at 6 and 7 days. We can conclude that progesterone and LH measurements in bloodstains at least 1 week old and probably much older can be very useful in establishing the diagnosis of pregnancy up until and including the time of birth.

Efficacy of cerebro-spinal fluid biochemistry in the diagnosis of brain insult.

Postmortem biochemical indices may provide a useful adjunct to morphological studies in the identification of antemortem brain insult. We studied 34 routine medico-legal cases categorising them into one of four diagnostic groups. There were 11 cases of head trauma, 7 of 'hypoxia' (3 hangings and 4 carbon monoxide or drug poisonings), 7 sudden cardiac deaths and 9 miscellaneous cases. Survival time and postmortem interval was known for each case. The degree of cranio-cerebral trauma was graded. Cerebro-spinal fluid (CSF) and vitreous humour were analysed for calcium, glucose, total proteins, aldolase, aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyltransferase (GGT), lactate dehydrogenase (LDH), creatine kinase (CK) and creatine kinase BB isoenzyme (CK-BB). CK-BB was also measured in superior vena cava serum. In CSF there was a significant correlation between the severity of cranio-cerebral trauma and levels of aldolase, CK-BB, AST, ALT and total proteins. CSF CK-BB, median units/l (range), for the groupings of head trauma, hypoxia, sudden cardiac death and miscellaneous were respectively 823 (2-3431); 96 (2-187); 4 (2-25); 5 (1-69). Corresponding serum CK-BB levels were 240 (28-322); 390 (26-411); 180 (20-482); 79 (18-530).

Study of the enzymatic activity of GGT, LDH, PAP and PSA in semen stains: application to age calculation.

The conditions under which semen stains are stored can markedly affect the stability of some of their biochemical parameters. Because of the resistance of semen to processes of degradation and denaturation, the age of the stain may, under optimum conditions, be calculated. We studied six series of semen stain placed on absorbent natural cloth under three different storage temperatures: 5 degrees C (refrigerator), 18-25 degrees C (room temperature), and 38 degrees C (incubator). Seminal fluid was obtained from nonvasectomized and vasectomized individuals aged 25-40 years (mean age 30 +/- 0.32 years, SD 3.06). A total of 240 strains were divided into groups depending on the duration of storage: 24, 48 or 72 h, 1 week, 1, 2, 4 or 6 months. Eluates were analyzed for gamma glutamyltransferase (GGT), lactate dehydrogenase (LDH), prostatic acid phosphatase (PAP), and p30 or prostate specific antigen (PSA). The results of regression analyses showed that a large proportion of the dependent variable (age of the stain) was explained by the rest of the biochemical markers tested. Calculation of the age of the semen stain thus requires consideration of several biochemical parameters.

Mortality in maximum security psychiatric hospital patients.

Numerous studies have mentioned to the high percentage of violent deaths in prison psychiatric hospitals, with suicide being the principal cause. The aim of this study was to analyze the circumstances related with the deaths recorded in a high security institution. Postmortem reports on all the deaths at the Alicante Psychiatric Prison between 1984 and 1997 were studied (36 cases of unnatural death and 28 of natural death). Of the violent deaths recorded 34 (94.4%) were suicides. Demographic, clinical and interpersonal variables factors were registered. In the cases of suicides, the method used, the place of death, season, month and time of suicide were analyzed. In our study, 64.7% of suicides were schizophrenic and 32.4% had inflicted self-harm previously. We found a statistically significant association between the cause of death (natural, suicide or homicide) and age, 47.1% of suicides being between the ages of 18 and 30 and 29.4% between the ages of 30 and 45. Natural causes predominated in older subjects. The prison population studied showed grave negative traits, mental illness and criminal behavior having forced them to the very edge of society. Our results were compared with the death and suicide rates of the general Spanish population.

Diagnostic efficacy of biochemical markers in diagnosis post-mortem of ischaemic heart disease.

In forensic medicine, there is a need for more sensitive biochemical markers for the post-mortem diagnosis of acute myocardial infarction. A study of the distribution of biochemical markers in different fluids is of great significance in post-mortem diagnosis, because their distribution depends on the location of tissue damage and release kinetics. The aim of this study is to compare the sensitivities and specificities of creatine kinase-MB (CK-MB), myoglobin and cTnI in serum and pericardial fluid for the post-mortem diagnosis of acute myocardial infarction (AMI). We studied 188 cadavers selected during 1 year from medicolegal autopsies. The groups were as follows: (1) myocardial infarction (n = 52); (2) asphyxia (n = 59); (3) multiple trauma (n = 41); (4) natural deaths excluding myocardial infarction (n = 36). We obtained statistically significant differences in pericardial fluid for all the biochemical markers, the highest levels being obtained in the group of cadavers who had died from myocardial infarction. A common factor is the high negative predictive value found in biochemical markers, which is contrary to the findings obtained in clinical practice, when the percentages of sensitivity are very high.

Epidemiology of suicide in elderly people in Madrid, Spain (1990-1994).

The progressive aging of the population as a whole, the frequent appearance of degenerative diseases, and the greater frequency of suicide among persons older than 65 years than in younger age groups, are worrisome issues that deserve investigation. The aim of this study was therefore to analyze different epidemiological and social factors that influence suicide behaviour in elderly subjects in Madrid (Spain) during a 5-year period from January 1990 to December 1994. Post-mortem reports on all deaths that were examined at the Institute of Forensic Medicine in Madrid were studied. All cases of suicide in subjects aged > or = 65 years during these years (N = 461) were studied through the autopsy records and information from the coroner's inquest. Variables corresponding to demographic, clinical and interpersonal factors, method of suicide, scene of death, season, month and time of suicide were registered. In both sexes, jumping from a height was the most frequent method (63.6%). Family members had noted symptoms of depression in almost half of the cases (49.5%). Coexisting physical disorders were present in 68.9% of the subjects. Health care professionals have an important role to play in-suicide prevention.

Vitreous humor carbohydrate-deficient transferrin concentrations in the postmortem diagnosis of alcoholism.

Deaths from the effects of alcohol intoxication are encountered routinely in forensic practice. In an important number of cases difficulty may arise in interpreting the significance of results obtained in the autopsy. In clinical practice biochemical markers, particularly serum gamma-glutamyl-transpeptidase (GGT), alanine aminotransferase (ALT), aspartate transaminase (AST), carbohydrate-deficient transferrin (CDT), and erythrocyte mean corpuscular volume are used to diagnose heavy alcohol consumption. CDT is used as a reliable and specific marker. In postmortem diagnosis, because of the difficulty in interpreting blood alcohol levels and relatively non-specific pathological features, biochemical compounds have been studied for use as possible markers. The aim of this study was to evaluate the usefulness of the postmortem determination of CDT in vitreous humor as a confirmation of antemortem alcoholism. CDT levels were studied in 66 male cadavers with a mean age of 55.9 years (S.D. 17.0, range 22-87 years) with a mean postmortem interval of 17.9 h (S.D. 11.4, range 4-72 h). Cases were assigned to two diagnostic groups according to the antemortem diagnosis of alcoholism. Statistically significant differences were found for CDT and ALT concentrations between the two diagnostic groups. The highest vitreous humor levels of CDT and ALT were obtained in the group of cases with a previous diagnosis of alcoholism. Our results suggest that vitreous humor CDT levels are useful in cases where the postmortem diagnosis of alcoholism is hindered by the non-specificity of data.

A case of self-inflicted wounding by the introduction of needles through the abdominal wall to induce abortion.

The self-inflicted wounding of a 23-year-old woman who introduced needles through the abdominal wall to induce abortion is reported. The woman first came to hospital with metrorrhagia and pain in the right iliac fossa. Initially she refused treatment and went home. Twenty-two hours later she was admitted to hospital after giving birth at home to a male fetus of 610 g. The placenta was expelled in the hospital and a sewing needle was found. Lateral and anteroposterior radiographs revealed 15 such needles in the hypogastric region, most of them at subcutaneous level. The newborn was admitted to the intensive care unit in a generally poor condition, with hypothermia, cyanosis and bradycardia. An X-ray showed a metallic object in the abdominal region which, again, corresponded to a sewing needle. The newborn did not respond to treatment and died 2h after admission.

Behavior at school and social maladjustment.

School maladjustment is one of the first symptoms to appear in response to alterations in the child's development and maturation. The aim of this paper is to study school maladjustment in minors confined in juvenile court center and its associations with sociofamiliar, psychobiographical, and social maladjustment variables among others, given the fact that school is one of the earliest agents of socialization. The results point to the influence of family conflict on the subject's behavior in association with the anxiety trait and extroversion. We believe that an accurate diagnosis of school maladjustment is essential as it is in this setting where the treatment of social maladjustment can be initiated with a minimum of delay.

Personality traits in juvenile maladjustment.

The aim of this study was to establish the role of some personality traits, measured by psychological tests, in minors and adolescents experiencing problems in their social integration. We studied 189 subjects (110 male, 79 female) ranging in age from 11 to 18 years. All the subjects were from centers under the administration of the juvenile court of Murcia, Spain. Each subject underwent individual clinical psychological examination as well as psychometric and psychological studies. Our results show a statistically significant association between the scores on some of the tests used and variables related to familial and social maladjustment. These personality traits define a set of individuals with a characteristic profile, for whom unfavorable biographical events have made integration in, and adaptation to, the established social milieu difficult.

Family violence as a determinant factor in juvenile maladjustment.

The family is one of the major socialization agencies for children. Parents are one of the most important models from whom the child and adolescent acquire a wide variety of behavior patterns, attitudes, values, and norms. The aim of this study was to determine the importance of factors related to family conflicts in the genesis of social maladjustment. A total of 189 young people (110 boys and 79 girls) from training schools connected with the juvenile court in Murcia (Spain) were studied. The subjects' ages ranged from 11 to 18 years (mean 13.51, SD 0.16). Our sample comprised a group of minors who experienced a high incidence of intrafamilial pathology, which was found to be a significant discriminant factor. Aggressive behavior, rules, norms, values, opinions, and attitudes toward aggressivity can be learned in the family setting.

[Pancoast syndrome caused by lung tuberculosis]. / Síndrome de Pancoast producido por tuberculosis pulmonar.

Pancoast's syndrome arises from neoplasms in 95% of cases but infection is a rare cause. We describe a patient with Pancoast's tumor secondary to tuberculosis. Pain caused by plexopathy and lack of diagnosis by noninvasive means led to the need for open biopsy.