Effects of topical Catalase application on dental pulp tissue: a histopathological evaluation.

OBJECTIVES: recently, the role of antioxidant enzymes in wound healing and their therapeutic potential has been a subject of interest in medical sciences. However there is restricted knowledge on the results of topical application, optimal therapeutic dosage and carrier types. The purpose of this study was to investigate the wound healing response of dental pulp after direct pulp capping with an antioxidant enzyme; Catalase (C-40). METHODS: three mongrel dogs were used in this study. A total of 32 perforated Cl V preparations were treated with either 0.8mg (n=16) or 1.6mg (n=16) Catalase (C-40) which was incorporated in gelatin carriers whereas 16 control teeth had carriers without Catalase. One millimeter thick Teflon disks were placed over the gelatin pellets and the cavities restored with silver amalgam. Short term and long term histopathologic reactions were evaluated after 7 and 90 days. RESULTS: there were no statistically significant differences between the inflammatory cell responses of control and either dosage groups of Catalase at the 7-day evaluation period. However, after 90 days, better tissue healing was observed in the teeth treated with Catalase, resulting in significant differences with the control group. CONCLUSIONS: it can be concluded that the results of this study encourage the topical use of antioxidant Catalase as a pulp-capping agent. However, further studies are required to determine optimal antioxidant dosages.

Tissue reaction to three subcutaneously implanted local hemostatic agents.

Soft tissue response to three subcutaneously implanted local hemostatic agents; oxidised regenerated cellulose (Surgicel), gelatin sponge (Spongostan) and collagen sponge (Hemostagen) were evaluated histopathologically 7, 14, 21, 30 and 45 days following their implantation in rats. The results showed that all materials were well tolerated by soft tissues. These materials neither seemed to impair nor contribute to wound healing.

Mandibular soft tissue mass as the initial presentation of Burkitt's lymphoma.

A six-year-old girl was evaluated for an intraoral mass of 5 months duration, which had been treated with antibiotics in a district hospital. Extraoral examination showed a firm, non-tender swelling at the angular region of the left mandible with regional lymphadenopathy. Intraoral examination revealed a mass extending from the left mandibular primary first molar to the retromolar region, interfering normal occlusion. The mass was painful and firm, peduculated and reddish in appearance. The remainder of the oral examination was unremarkable. Panoramic radiograph and CT scan showed a large multilocular radiolucency beginning from the bud of second premolar, extending to the mandibular ramus that displaced the bud of second molar. Incisional biopsy of the intraoral mass was performed and the result was reported as Burkitt's lymphoma. The child was treated with chemotherapy and radiation, with recurrence.

Comparison of electrosurgery and formocresol as pulpotomy techniques in dog primary teeth.

The primary teeth of four dogs were treated using either a conventional formocresol or electrosurgical technique following pulpotomy. Pulps of the treated teeth were evaluated histologically for the presence of inflammation, fibrosis, necrosis, resorption, and reparative dentin formation. Results indicated that conventional formocresol pulpotomy technique is histopathologically superior to electrosurgery pulpotomy technique.

[Guided tissue regeneration in degree III furcation involved teeth]. / Yönlendirilmis doku rejenerasyonu teknigi ile Klas III furkasyon defektlerinin tedavisi.

Present investigation was designed to evaluate the regenerative potential of the periodontal ligament tissue in degree III furcation defects. The upper 2. and 3. premolar teeth of 3 dogs were used. Periodontal breakdown was induced around the teeth by experimental periodontitis model. After the breakdown occurred, mucoperiostal flaps were elevated and granulation tissue removed. The roots were curretted and cementum of the periodontitis involved parts of the roots was removed. The resorbable material was trimmed to approximate size needed to cover the root surfaces. The material provisionally adapted to the both buccal and palatinal root surfaces and flaps were sutured. After 42 days of post operative period histological examination of experimental and control groups showed that chances of obtaining new connective tissue attachment may be enhanced in treatment of Cl III furcation defect by Guided Tissue Regeneration.

Bone reaction to subperiosteally implanted hydroxyapatite/collagen/glycosaminoglycans and coral in the guinea pig.

Tissue reactions and new bone formation at 30, 60, and 90 days after the subperiosteal implantation of hydroxyapatite/collagen/glycosaminoglycans and blocks of coral in 18 guinea pigs were evaluated histologically with the use of polarized light and light microscopy. Animals implanted with coral showed new bone formation at each time interval and resorption of coral was observed. Histopathologic evaluation of hydroxyapatite/collagen/glycosaminoglycans implants revealed no evidence of new bone formation and the hydroxyapatite particles were surrounded by fibrous connective tissue.

[Effects of citric acid and tetracycline HCL treatment on periodontal healing]. / Sitrik asit ve tetrasiklin HCL uygulamalarinin periodontal iyilesme üzerine etkileri.

The aim of the present study was to examine if a new connective tissue attachment is established following citric acid and tetracycline HCI treatment of roots, surgically deprived of its attachment apparatus. The 2. and 3. premolars of three dogs were used. After elevation of mucoperiostal flaps alveolar bone was removed extending a level of 2/3 of the roots. Then, teeth were treated with %1 citric acid PH 1 and Tetracycline HCI (Aquas solution) for 3 minutes and 4 teeth were used as control. After the 21 days of healing period the groups were evaluated histopathologically. The results indicate that citric acid and tetracycline treatment increase the amount of new attachment, inhibit the down growth of the dento gingival epithelium, allowing more time for the repopulation of periodontal ligament cells on the root surfaces.

[Alveolar cavitational osteopathosis--a case report]. / Alveoler kavite osteopatozu. Vaka raporu.

Alveolar cavitational osteopathosis is a rare disease of dental alveolus with an undefined origin. Clinically, the symptoms may vary widely; patients may complain dental sensitivity in those teeth which appear normal or pain remote from the craniofacial region. In this case report, present knowledge about alveolar cavitational osteopathosis and diagnostic and treatment process of our case was presented.

Lymphoepithelial cyst.

Lymphoepithelial cyst is thought to arise from the entrapment of salivary epithelium in lymphoid aggregates during embryogenesis. Such lesions are not common. It has been noted that there is a close association between the oral mucosal lymphoid tissue and the secretory ducts of minor salivary glands which is recognized as "Duct-associated lymphoid tissue" (DALT). A case of lymphoepithelial cyst with histologically documented close proximity between the excretory ducts of the minor salivary gland and lymphoid tissue in the cyst wall suggesting a DALT-like structure is presented.

Effects of fibrin adhesive material (Tissucol) application on furcation defects in dogs.

A histological study was conducted to evaluate the effects of fibrin adhesive material (FAM) application on periodontal healing in seven experimental dogs. Bilateral class III furcation defects were surgically created at the second and third premolars, then orthodontic wires were placed around the teeth to induce periodontitis. Six weeks later, the wires were removed and the defects were treated by either surgery alone or surgery plus FAM application. A total of 21 specimens were obtained for histological and histometrical analysis on days 7, 21 and 42. The Mann-Whitney U test showed significantly more new attachment and bone regeneration in the FAM-treated group compared to the control (p less than 0.05). Surgery plus FAM application in the treatment of class III furcation defects seemed to be effective in promoting connective tissue attachment and bone regeneration.

Oral congenital dermoid cyst in the floor of the mouth of a newborn. The significance of gastrointestinal-type epithelium.

A case of congenital dermoid cyst in the floor of the mouth in a newborn is presented. The presence of microcyst formation with intestinal-type epithelial lining and mucin histochemistry are discussed.

Odontogenic myxoma containing osteocement-like spheroid bodies: report of a case with an unusual histopathological feature.

The odontogenic myxoma is a rare, benign, but locally invasive tumour of the jaws. Radiographically, it is a bone destroying lesion and has ill-defined borders. Histological characteristics are spindle and stellate-shaped tumour cells and a distinct myxomatous stroma. Bony islands that represent residual trabeculae are found scattered throughout the lesion. This report describes a case of odontogenic myxoma that shows diffusely dispersed osteocement-like spherular calcified bodies, unlike residual bone trabeculae, and discusses its differential diagnosis.

The effect of a collagen membrane in regenerative therapy of two-wall intrabony defects in dogs.

Guided tissue regeneration that supports the periodontal ligament and bone cells in achieving healthy attachment between teeth and alveolar bone following periodontal therapy has been repeatedly described in the literature. The aim of the present study was to assess the effect of an absorbable collagen membrane used in guided tissue regeneration procedures in two-wall intrabony defects. For this purpose, periodontal defects were surgically created around mandibular teeth in nine dogs. In a randomly chosen quadrant in each dog, a collagen membrane was shaped to cover the interproximal bone defect and adjacent root surface. No collagen membrane was placed over the control defects. Block biopsies of test and control sites were obtained from three dogs at 30 days, three dogs at 60 days, and three dogs at 90 days after the procedures. Histomorphologic and histometric evaluations were performed. We observed that both collagen membrane treated and control defects demonstrated similar amounts of new attachment and bone. However, gingival recession and postoperative keratinized tissue loss were observed in most of the sites. Although there was a tendency towards new attachment in both groups, the gingival tissue loss due to recession led to limited regeneration.

[Schwannoma (neurilemmoma): a case report]. / Schwannoma (neurilemoma): bir vaka raporu.

Neurilemmoma, neurofibroma and neuroma are benign neoplasms of nerve tissue. These arise from components of the peripheral nerves. They are extremely rare within the jaws. Schwannoma or neurilemmoma may occur along the route of any of the peripheral cranial or sympathetic nerves. The source of origin is thought to be the schwann cells. Its appearance in oral and paraoral regions is not rare. Treatment is surgical and the prognosis is excellent. It has no tendency toward recurrence. Our schwannoma case is found noteworthy because of its localisation and subsequent complications and is presented to your concern.

[Oral findings in dyskeratosis congenita (a case report)]. / Dyskeratosis congenita'a agiz bulgulari.

Dyskeratosis congenita is a rare genokeratosis and characterized by, A) Reticular dermal atrophy and telangiectatic pigmentation, B) Distrophies of the nails, C) Oral leukokeratosis. In this article, a 22 year old male patient with Dyskeratosis congenita was presented and oral findings were discussed.

Keratinization of palatal mucosa beneath metal-based removable partial and acrylic-based complete dentures compared with normal palatal mucosa: a clinical, cytological and histological study.

Normal human palatal epithelium is an orthokeratinizing tissue with a well-developed stratum corneum. Several cytological and histological studies showed that when the palate covered by a denture fully or partially, keratinization of the mucosa changes. The results of some studies suggested that keratinization changes from orthokeratosis to parakeratosis, and there is a reduction in the thickness of stratum corneum. Whereas the results of some other studies showed increased orthokeratinization. This study was planned to determine changes in keratinization of palatal mucosa in denture wearers compared with normal palatal mucosa. Keratinization of palatal mucosa was examined by mainly exfoliative cytology in three groups. Group-1 consisted of 51 patients wearing metal-based removable partial denture; group-2 consisted of 50 patients wearing acrylic-based complete denture and group-3 including 57 healthy volunteers who have not been wearing denture served as the controls. In each group, the results of exfoliative cytology were supported by histological examinations. Statistical results showed that either acrylic-based complete or cobalt-chromium-based removable partial dentures seem to reduce the quantity and/or quality of the keratin layer. The mean values of keratinization and the thickness of stratum corneum were significantly lower in the complete denture group than in the partial denture group. Furthermore the reliability of exfoliative cytology was confirmed by the guidance of histological examination.