RESUMO
A putative role of the brain-derived neurotrophic factor (BDNF) in epilepsy has emerged from in vitro and animal models, but few studies have analyzed human samples. We assessed the BDNF expression of transcripts with exons I (BDNFI), II (BDNFII), IV (BDNFIV) and VI (BDNFVI) and methylation levels of promoters 4 and 6 in the hippocampi of patients with pharmaco-resistant temporal lobe epilepsy (TLE) (n=24). Hippocampal sclerosis (HS) and pre-surgical pharmacological treatment were considered as clinical independent variables. A statistical significant increase for the BDNFVI (p<0.05) was observed in TLE patients compared to the autopsy control group (n=8). BDNFVI was also increased in anxiety/depression TLE (N=4) when compared to autopsies or to the remaining group of patients (p<0.05). In contrast, the use of the antiepileptic drug Topiramate (TPM) (N=3) was associated to a decrease in BDNFVI expression (p<0.05) when compared to the remaining group of patients. Methylation levels at the BDNF promoters 4 and 6 were similar between TLE and autopsies and in relation to the use of either Sertraline (SRT) or TPM. These results suggest an up-regulated expression of a specific BDNF transcript in patients with TLE, an effect that seems to be dependent on the use of specific drugs.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/genética , Epilepsia do Lobo Temporal/genética , Hipocampo/metabolismo , Adulto , Idoso , Anticonvulsivantes/uso terapêutico , Metilação de DNA , Epilepsia do Lobo Temporal/tratamento farmacológico , Éxons , Feminino , Frutose/análogos & derivados , Frutose/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade , Regiões Promotoras Genéticas , RNA Mensageiro/metabolismo , Sertralina/uso terapêutico , Topiramato , Adulto JovemRESUMO
"The study deals with immigrants' integration process in western societies and the ensuing relationship between cultural and economic aspects. Receiving countries are inclined to classify immigrants according to their ethnic origins. This policy is bound to have major consequences not only on social but also on occupational levels. Ethnicity deeply affects the labour-market and is responsible for a negative influence on the general integration process." (SUMMARY IN ENG AND FRE)