[The concept of identity in relation to motherhood in the discourse of teenagers who attend a pregnancy from a linguistic and psychoanalytical perspective]. / El concepto de identidad en relación con la maternidad en el discurso de adolescentes que cursan un embarazo desde una perspectiva lingüística y psicoanalítica.

My aim in this paper is to briefly discuss the notion of identity and how can unify the Psychoanalytical concept with the discursive, in order to carry out multidisciplinary research on this topic. This work is based on life stories collected in the Hospital Larcade, from 2008 to 2011, from teenagers who attend a pregnancy. Through the linguistic analysis of the guarantees, following the model of Toulmin (17), as it has been redefined by Pardo (14) and Molina (12), I intend to give an account of the discursive and psychological bases of what will then form an identity for these young women on maternity. They live in extreme poverty in the Buenos Aires suburbs and are combined with a large dose of family violence that links their identities as mothers to the image (or identities of the other) who have over their own mothers, parents, couples in these contexts of violence. These data are also confronted with domestic violence cases that appear in the media and how they are dealt with discursively at these. Thus realizes the contradictions imposed the cultural identity of a group (the viewers or readers of means, which are a large part of society) against the experience of violence of these mothers. This research has as theoretical framework the Critical Discourse Analysis and the methodology is qualitative.

A novel method for genotyping the Helicobacter pylori vacA intermediate region directly in gastric biopsy specimens.

The present report describes a novel method for genotyping the virulence-associated vacA intermediate (i) region of Helicobacter pylori in archive material. vacA i-region genotypes as determined by the novel method were completely concordant with those of sequence analysis and with those of functional vacuolation activity. The method was further validated directly in gastric biopsy specimens of 386 H. pylori-positive cases, and effective characterization of the vacA i region was obtained in 191 of 192 (99.5%) frozen and in 186 of 194 (95.9%) formalin-fixed paraffin-embedded gastric biopsy specimens, respectively. The genotyping method was next used to address the relationship between the vacA genotypes and the cagA status. The vacA i1 genotype was associated with vacA s1 (where s indicates signal region), vacA m1 (where m indicates middle region), and cagA-positive genotypes (P < 0.0001), while the vacA i2 genotype was closely related with vacA s2, vacA m2, and cagA-negative genotypes (P < 0.0001). The relationship between H. pylori vacA i-region genotypes and gastric disease development was subsequently evaluated in the Portuguese population. Patients infected with vacA i1 strains showed an increased risk for gastric atrophy and for gastric carcinoma, with odds ratios of 8.0 (95% confidence interval [CI], 2.3 to 27) and of 22 (95% CI, 7.9 to 63), respectively. Taken together, the results show that this novel H. pylori vacA i-region genotyping method can be applied directly to archive material, providing a fast evaluation of strain virulence determinants without the need of culture. The results further emphasize that the characterization of the vacA i region may be useful to identify patients at higher risk of gastric carcinoma development.

Increased immature hematopoietic progenitor cells CD34+/CD38dim in myelodysplasia.

BACKGROUND: Myelodysplastic syndromes (MDS) are clonal disorders affecting hematopoietic progenitor cells (HPC). Despite the relevance of clonal CD34+ cells in developing MDS, only few studies analyze the phenotype of this cell population. The aim of this study was to evaluate phenotypic changes on HPC in MDS that could reflect abnormalities in the differentiation process of stem cells. METHODS: We analyzed the expression of CD38 and HLA-DR on CD34+ cells by flow cytometry in 36 patients with MDS, as well as in healthy donors (n = 12) and patients with other hematological disorders: non-Hodgkin lymphomas and multiple myeloma, both in complete remission (CR) (n = 32); acute lymphoblastic leukemia in CR (n = 17); de novo acute myeloblastic leukemia (AML) at diagnosis (n = 22) and in CR (n = 37); and AML secondary to MDS at diagnosis (n = 19). Cases with available karyotype were grouped according to the International Prognostic Scoring System (IPSS). RESULTS: Compared to normal BM, the fraction of immature HPC, characterized as CD34+bright, intermediate FSC/SSC, and CD38dim, was significantly increased in high risk MDS and secondary AML, but not in low risk MDS, (P < or = 0.001, P = 0.03, and P = 0.7). De novo AML showed decreased immature HPC. High numbers of immature HPC correlated with higher IPSS risk groups (P = 0.05) and showed significant impact on disease progression (P = 0.03). CONCLUSION: Our study confirms that evaluation of CD38 expression pattern on HPC is an easy and reproducible test that allows evaluating the immature subset of progenitor cells. Increased immature HPC in high risk MDS and secondary AML may reflect blocked differentiation of CD34+ cells in these diseases.

El concepto de identidad en relación con la maternidad en el discurso de adolescentes que cursan un embarazo desde una perspectiva ling³ística y psicoanalítica / [The concept of identity in relation to motherhood in the discourse of teenagers who attend a pregnancy from a linguistic and psychoanalytical perspective].

My aim in this paper is to briefly discuss the notion of identity and how can unify the Psychoanalytical concept with the discursive, in order to carry out multidisciplinary research on this topic. This work is based on life stories collected in the Hospital Larcade, from 2008 to 2011, from teenagers who attend a pregnancy. Through the linguistic analysis of the guarantees, following the model of Toulmin (17), as it has been redefined by Pardo (14) and Molina (12), I intend to give an account of the discursive and psychological bases of what will then form an identity for these young women on maternity. They live in extreme poverty in the Buenos Aires suburbs and are combined with a large dose of family violence that links their identities as mothers to the image (or identities of the other) who have over their own mothers, parents, couples in these contexts of violence. These data are also confronted with domestic violence cases that appear in the media and how they are dealt with discursively at these. Thus realizes the contradictions imposed the cultural identity of a group (the viewers or readers of means, which are a large part of society) against the experience of violence of these mothers. This research has as theoretical framework the Critical Discourse Analysis and the methodology is qualitative.

Influencia del campo magnético estacionario en el cultivo del hibridoma IOR-A3 / Influence of the field magnetic stationary in the crop of the hybrid IOR-A3

Se aplicó un campo magnético estacionario con una intensidad de 4x10 a la -3 tesla durante una hora por seis días a cultivos celulares de hibridoma IOR-R3. Las células fueron cultivadas en plazas de 24 pozos a una densidad celular de 2x10 a la 4, y fueron mantenidas bajo condiciones de crecimiento exponencial a 37 grados Celsius y atmósfera de CO2 a 5 por ciento. se emplearon dos placas testigos y dos fueron sometidas a campo magnético. Durante el tiempo de exposición las placas testigos se mantuvieron a igual temperatura que las sometidas al campo. se analizó el crecimiento celular, viabilidad y concentración de inmunoglobulinas G obteniéndose mejores resultados en las células expuestas a campo magnético