RESUMO
Herein, we developed matrix metalloprotease (MMP) sensitive gold nanorods (MMP-AuNR) for cancer imaging and therapy. It was feasible to absorb NIR laser and convert into heat as well as visualize MMP activity. We showed the possibility of gold nanorods as a hyperthermal therapeutic agent and MMP sensitive imaging agent both in vitro and in vivo condition. The results suggested potential application of MMP-AuNR for simultaneous cancer diagnosis and therapy.
Assuntos
Carcinoma de Células Escamosas , Diagnóstico por Imagem/métodos , Ouro , Terapia a Laser/métodos , Metaloproteinases da Matriz/metabolismo , Fototerapia/métodos , Neoplasias Cutâneas , Animais , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/terapia , Linhagem Celular Tumoral , Inibidores Enzimáticos/farmacologia , Fluorescência , Ouro/química , Ouro/uso terapêutico , Temperatura Alta , Luz , Inibidores de Metaloproteinases de Matriz , Metaloproteinases da Matriz/química , Camundongos , Camundongos Nus , Microscopia Eletrônica de Transmissão , Nanotubos/química , Transplante de Neoplasias , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/terapia , Espectrometria de Fluorescência , Espectroscopia de Luz Próxima ao Infravermelho , TemperaturaRESUMO
Properties of ferritin, immobilized on dithiobis (N-succinimydyl propionate) (DTSP)-covered gold electrode, in 3-morpholino propanesulfonic acid buffer were investigated by AFM and FE-SEM. Electrochemical properties the ferritin was measured by a cyclic volatammetry. When the potentials of 0.2, 0.34, and 1.0V vs. Ag/AgCl were applied for 15h for the ferritin immobilization, the electrode potential of Fe(II)/Fe(III) in ferritin changed to negative values. Negative electrode potential shift of Fe(II)/Fe(III) in ferritin with respect to the applied potential could be attributed to the stabilized ferritin on DTSP-covered gold electrode. From AFM and SEM images, it was proven that ferritins fixed at below 0.34V were clusters in several micrometer and those fixed at higher applied potential than OCV were finely distributed particles in several tens of nanometer.