Identification of Single Nucleotide Polymorphisms as Biomarkers for Recurrent Pregnancy Loss in Korean Women.

BACKGROUND: Single nucleotide polymorphisms (SNPs) are reportedly associated with repeated abortion. Thus, genetic analysis based on race is the key to developing accurate diagnostic tests. This study analyzed the genetic polymorphisms of recurrent pregnancy loss (RPL) patients among Korean women compared to the controls. METHODS: In 53 women of RPL group and 50 controls, the genetic analysis was performed. The genotype distribution and allele frequency were analyzed statistically for the difference between the two groups. The association between each SNP marker and RPL risk was analyzed. RESULTS: The genotypes of LEPR, endothelial nitric oxide synthase (eNOS), KDR, miR-27a, miR-449b, and tumor necrosis factor-alpha (TNF-α) were analyzed using odds ratio (OR) with 95% confidence intervals (CIs). Only the AG genotype of miR-449b (A>G) polymorphism showed significant association with the risk of RPL when compared to the AA genotype (OR, 2.39). The combination of GG/AG+GG/CA+AA genotypes for eNOS/miR-449b/TNF-α was associated with 7.36-fold higher risk of RPL (OR, 7.36). The GG/AG+GG combination for eNOS/miR-449b showed 2.43-fold higher risk for RPL (OR, 2.43). The combination of AG+GG/CA+AA genotypes for miR-449b/TNF-α showed a significant association with the risk of RPL (OR, 7.60). From the haplotype-based analysis, the G-G-A haplotype of eNOS/miR-449b/TNF-α and the G-A haplotype of miR-449b/TNF-α were associated with increased risk of RPL (OR, 19.31; OR, 22.08, respectively). CONCLUSION: There is a significant association between the risk of RPL and miR-449b/TNF-α combination, and therefore, genetic analysis for specific combined genotypes can be an important screening method for RPL in Korean women.

Successful in Vitro Fertilization and Embryo Transfer after Transplantation of Cryopreserved Ovarian Tissue: Report of the First Korean Case.

For patients at risk of premature ovarian failure with cancer treatment, it is an important option to re-implant the ovarian tissue (OT) after cryopreservation to preserve endocrine function and fertility. With this technique, about 30% of pregnancy success rate and about 90 live births have been reported to date. However, there has been no case report of successful in vitro fertilization (IVF) and embryo transfer (ET) with oocytes collected from transplanted cryopreserved OT in Korea. We report a 30-year old woman with rectal cancer who underwent IVF and ET after cryopreserved OT thawing and re-implantation. She has been diagnosed with stage IIIC rectal cancer after surgery, and right ovary was removed and cryopreserved between cycles of chemotherapy. After completion of chemotherapy and radiotherapy, the patient underwent orthotopic transplantation of cryopreserved OTs. Three months after transplantation, the serum follicle-stimulating hormone level decreased from 91.11 mIU/mL to 43.69 mIU/mL. Thereafter, the patient underwent 11 ovarian stimulation cycles, and in 7 cycles, follicle growth was observed at the OT graft site. In one of these cycles, the oocyte was successfully retrieved and one embryo was transplanted after IVF. The patient was not pregnant, but the cryopreservation of OT can save the fertility after anticancer chemotherapy.

Direct hydrothermal synthesis of amine-functionalized cubic hematite (C-Fe2O3) and sonochemical deposition of nanosized Au for its application as a visible-light photocatalyst.

Cubic-shaped hematite (C-Fe2O3) functionalized with amine groups was directly prepared via one-pot hydrothermal reaction of Fe3+ with 1,12-diaminododecane (DA-12) in aqueous solution (50% ethanol). Herein, DA-12 (as a Lewis acid) promoted the aggregation of α-FeOOH nanorods with Lewis base sites, leading to the rapid recrystallization and conversion into uniform C-Fe2O3. C-Fe2O3 was subsequently deposited with nanosized Au via sonochemical reduction of 1.0 wt% HAuCl4 (0.1-0.8 mL), hereafter referred to as Au-deposited C-Fe2O3 (C-Fe2O3@Au). X-ray diffraction patterns of C-Fe2O3@Au confirmed the hexagonal crystalline phases of hematite and crystalline Au (111) and showed a weak broad band attributed to the amorphous carbon of DA-12. C-Fe2O3@Au was tested as a visible-light photocatalyst towards the degradation of methylene blue (MB) dye. C-Fe2O3@Au (0.1-0.4 mL of 1.0 wt% HAuCl4) exhibited 6-8 times higher photocatalytic activity than the Au-free counterpart (C-Fe2O3). The enhanced photocatalysis was mainly attributed to the improved separation efficiency of photo-excited charge carriers, i.e., the facilitated transport of electrons from the conduction band to the lower lying Fermi level of Au. However, the photocatalytic activity of C-Fe2O3@Au (0.8 mL of 1.0 wt% HAuCl4) was decreased probably due to the reduction of active sites for MB adsorption by the high coverage of the Au layer. The combined hydrothermal and sonochemical methods provided the direct synthetic route to cubic-shaped hematite decorated with nanosized Au and surface amine functionality as a promising visible-light photocatalyst.