Salivary and serum concentrations of selected pro- and antiinflammatory cytokines in relation to oral lesions among children undergoing maintenance therapy of acute lymphoblastic leukemia.

Acute lymphoblastic leukemia is the most common type of childhood cancer, accounting for about 23% of all cancers diagnosed in this age group. The last stage of radical treatment is remission maintenance, during which hospitalization is not necessary. The lesions occurring in the oral cavity caused by medications and chemotherapy may also be directly related to hematological and systemic disorders. The aim of this study was to examine the levels of selected pro- and anti-inflammatory cytokines in saliva and serum of both patients undergoing remission maintenance and those after the cessation of therapy who reported to the hematology clinic of the Pediatric University Hospital in Lublin. The results were later analyzed in relation to the frequency of oral lesions and subjective intensity of oral complaints. The study revealed significant differences in salivary and serum concentrations of TNF-α and IL-10 between test and control groups. Oral lesions were more frequent in patients receiving therapy compared to the control group. Subjective afflictions described by the Visual Analog Scale (VAS) mean values were highest in the control group.

Severity of work in opinions of rural women living in the Bieszczady region of south-eastern Poland.

OBJECTIVE: The objective of the study was recognition of the opinions of rural women living in the Bieszczady region of south-eastern Poland, concerning their perception of the degree of severity of work. The study was conducted among the inhabitants of the Cisna commune. MATERIAL AND METHODS: The basic research instrument was a questionnaire form containing 15 items. The study covered 101 women living in the Cisna commune in the Bieszczady. The self-reported degree of work load on a farm was analyzed among women who were occupationally active, and those who were not occupationally active. The effect of assistance from family members on the severity of work perceived by women was considered. RESULTS: The women in the study focused on agro-tourism, a few of them were engaged in agricultural work and the majority worked in household gardens. The study showed that occupationally active women work considerably longer, on average, and are the most loaded, compared to those not engaged in occupational activity. The mean daily time devoted to duties on a farm did not significantly differ between occupationally active and non-active women, and amounted to 380 and 320 minutes, respectively. The majority of women who were non-active occupationally evaluated their household chores as the highest work load. In turn, the women who undertook occupational activity assessed them as most burdensome, despite great help from their family. The body positions assumed while performing field work were: standing, standing-bent-over, and walking. The mean evaluations of work load while standing and standing-bent-over did not significantly differ, but affected the work load among women who, however, could not assess the severity of their work resulting from the body position assumed.

Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patients.

INTRODUCTION: The main mediators of the sympathetic nervous system in the effectors part are catecholamines (CA). An increased sympathetic nerve activity observed in chronic kidney disease (CKD), is due to a raised level of CA in plasma. Renalase is a protein secreted by the kidneys, composed of 342 amino acids, which is able to metabolize the circulating CA and possibly play an important role in the regulation of sympathetic tone and blood pressure. Also, oxidative stress, defined as a disruption of the equilibrium between the generation of oxidants, is a crucial factor in the development of the inflammatory syndrome associated with CKD. The advanced oxidation protein products (AOPP) represent exquisite markers of phagocyte-derived oxidative stress. OBJECTIVE: The aim of the study was to investigate the concentration of renalase and explore the associations between AOPP with regards to CA in haemodialysis (HD) patients. MATERIAL AND METHODS: The study was conducted among 50 residents of the municipality and neighbouring villages in the province of Lublin, central-eastern Poland. RESULTS: In the studied patients, it was found that an average concentration of renalase was 44.8 ± 6.5 µg/mL, whereas of AOPP plasma levels - 57.5 ± 21.5 µmol/L. The results demonstrated the correlation between levels of renalase and AOPP in the HD patients. Indeed, elevated levels of renalase and AOPP in HD may be due to the presence of uremic toxins in blood. The concentration of urea affects the plasma concentrations of AOPP and renalase causing a direct relationship between renalase and AOPP. However, there is no clear relationship between renalase and circulating catecholamines in HD patients.

Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients.

BACKGROUND: microRNA (miRNA) belongs to the non-coding RNAs family responsible for the regulation of gene expression. Renalase is a protein composed of 342 amino acids, secreted by the kidneys and possibly plays an important role in the regulation of sympathetic tone and blood pressure. The aim of the present study was to investigate plasma renalase concentration, and explore the relationship between miRNA-146a-5p expression and plasma renalase levels in hemodialyzed patients. METHODS: The study population comprised 55 subjects who succumbed to various cardiac events, 27 women and 28 men, aged 65-70 years. The total RNA including miRNA fraction was isolated using QiagenmiRNEasy Serum/Plasma kit according to the manufacturer's protocol. The isolated miRNAs were analyzed using a quantitative polymerase chain reaction (qRT-PCR) technique. The plasma renalase levels were measured using a commercial ELISA kit. RESULTS: In the group of patients with high levels of renalase, higher miRNA-146a expression was found, compared with those with low concentration of renalase. Patients with simultaneous low miRNA-146a expression and high level of renalase were confirmed to deliver a significantly longer survival time compared with other patients. CONCLUSIONS: miRNA-146a and plasma renalase levels were estimated as independent prognostic factors of hemodialyzed patients' survival time. Patients with low miRNA-146a expression demonstrated a significantly longer survival time in contrast to the patients with a high expression level of miRNA-146a. Moreover, a significantly longer survival time was found in patients with high renalase activity compared with patients with low activity of the enzyme.

Relationship between renalase and N-terminal pro-B-type natriuretic peptide (NT pro-BNP) in haemodialysis patients.

INTRODUCTION: Our knowledge in the field of cause of deaths in dialysis patients is rapidly expanding, yet we still do not fully understand how renalase regulates the processes of cardiovascular disease developing in end-stage renal disease. Increased sympathetic nerve activity observed in chronic kidney diseases due to raised catecholamines in plasma results from the absence of renalase. Renalase synthesized and secreted by the kidneys participate in the regulation of sympathetic tone and blood pressure. A family of natriuretic peptides has been identified - NT pro-BNP - which seems to be the best predictor of clinical outcome and marker of extracellular fluid overload, as well as predicting mortality, irrespective of renal function. OBJECTIVE: The aim of the presented study was to investigate renalase concentration and investigate associations between NT-proBNP, as well as analyzed parameters in haemodialysis patients. MATERIALS AND METHOD: The study was conducted among residents of the municipality and neighbouring villages in the province of Lublin, central-eastern Poland. 49 male subjects on haemodialysis, aged 65.3 ± 14.2 years, median time on haemodialysis: 37.5 months, were included. All study subjects underwent haemodialysis 3 times a week. The mean concentration of renalase in the entire study population was 126.59 ± 32.63 ng/mL. The circulating levels of NT-proBNP was 813.64 ± 706.96 pg/mL. A significant inverse correlation was found between NT-proBNP and renalase plasma levels (R = -0.3, P = 0.03). CONCLUSIONS: Inverse correlation between NT-proBNP and renalase plasma levels in haemodialysis patients were due to impaired kidney function, accompanied by increased sympathetic nerve activity, which have an impact on the development of hypertension and cardiovascular complications.

Analysis of situation of rural women in the Lublin Region from the aspect of loading with work.

OBJECTIVE: The objective of the study was recognition of rural women's opinions concerning the degree of heaviness of work activities performed and obtaining the answer to the question: What is the actual level of loading rural women with work? METHODS: The basic research instrument was a questionnaire form. A representative group of women aged 40-50 were selected for the study because this group had the most complete family structure. The study was conducted among rural women living in the commune of Zwierzyniec. Family size and multi-generationality were analyzed, as well as family members' assistance in household and field activities. The type of work performed was analyzed with consideration of the duration of work and body position while performing this work. Self-reported degree of loading with work on a farm was analyzed, and effective energy expenditure calculated for individual work activities performed by women, based on which the actual degree of loading with work was determined. RESULTS: The respondents generally perceived their loading with work as low. They considered household jobs as not loading with or not exerting any effect on their fatigue and state of health. A very weak statistical relationship was observed between the duration of loading with household jobs and the perceived degree of loading with this work. The Kolmogorov-Smirnov test showed that the evaluations expressed by women concerning the degree of loading with household chores did not depend on time devoted to these work activities. The majority of women similarly evaluated work load, irrespective of the actual degree of loading. Work activities performed in a household were classified according to work load as heavy, medium-heavy and mediocre. Subjective evaluations of work load by rural women considerably differed from the assessment performed using the work time schedule method.

Level of glycation gap in a healthy subject.

INTRODUCTION: The discordance between glycated hemoglobin (HbA(1C)) and fructosamine (FA) estimations in the assessment of glycemia is often encountered. A number of mechanisms might explain such discordance, but whether or not they are consistent is uncertain. Nevertheless, the fact that there is a discrepancy in HbA(1C) and mean blood glucose cannot be ignored in the monitoring of glycemic control. To address the discrepancy between HbA(1C) and mean blood glucose, Robert Cohen proposed the measurement of glycation gap (GG). Recently, the 'Glycation Gap' (GG) has been defined as the difference between the measured HbA(1C). GG has improved the quality of the monitoring of glycemic control, especially for those patients whose HbA(1C) levels do not truly reflect the mean blood glucose levels. OBJECTIVE: The aims of the statistical analyses were to estimate GG values in a healthy subject. The research was conducted among the inhabitants of the Zwierzyniec commune and nearby villages. MATERIAL AND METHODS: The study population consisted of 93 subjects: 63 women and 30 men, between the ages of 18-79. Measurements of HbA(1C) and FA in the 93 people were used to calculate GG, defined as the difference between measured HbA(1C) and HbA(1C) predicted from FA, based on the population regression of HbA(1C) on FA. CONCLUSIONS: In considering the values GG in the study group, particular significance should be attributed to a progressive increase of GG with advancing age. Elderly people who are at risk of developing diabetes, or who have already developed the disease, may not exhibit the classic symptoms expected. Age-related changes can mean that some symptoms will be masked, or more dificult to spot. It is worth pointing out that HbA(1C) together with GG must be taken into account in the correct interpretation of the glycation processes.