RESUMO
In this study the potential of new imaging techniques such as Magnetic Resonance Imaging (MRI), Matrix-Assisted Laser Desorption/Ionization (MALDI) profiling mass spectrometry ("MALDI Profiling") and Fourier Transform Infrared (FTIR) spectroscopic imaging was evaluated to study morphological and molecular patterns of the potential medicinal fungus Hericium coralloides. For interpretation, the MALDI profiling, FTIR imaging and MRI results were correlated with histological information gained from Scanning Electron Microscopy (SEM) and Light Microscopy (LM). Additionally we tested several evaluation processes and optimized the methodology for use of complex FTIR images to monitor molecular patterns. It is demonstrated that the combination of these spectroscopic methods enables to gain a more distinct picture concerning morphology and distribution of active ingredients. We were able to obtain high quality FTIR imaging and MALDI-profiling results and to distinguish different tissue types with their chemical ingredients. Beside this, we have created a 3-D reconstruction of a mature Hericium basidioma, based on the MRI dataset: analyses allowed, for the first time, a realistic approximation of the "evolutionary effectiveness" of this bizarrely formed basidioma type, concerning the investment of sterile tissue and its reproductive output (production of basidiospores).
Assuntos
Basidiomycota/química , Basidiomycota/citologia , Química Farmacêutica , Imagem Molecular , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
AIM: We compared the MBEC™-HTP assay plates made of polystyrene with metal discs composed of TMZF(®) and CrCo as substrates for biofilm formation. METHODS AND RESULTS: Staphylococcus aureus was grown on polystyrene and on metal discs made of titanium and chrome-cobalt. Antibiotic susceptibility was assessed by examining the recovery of cells after antibiotic exposure and by measuring the biofilm inhibitory concentration (BIC). The minimal inhibitory concentration (MIC) was assessed with planktonic cells. Bacterial growth was examined by scanning electron microscopy. The antibiotic concentration for biofilm inhibition (BIC) was higher than the MIC for all antibiotics. Microscopic images showed the biofilm structure characterized by groups of cells covered by a film. CONCLUSIONS: All models allowed biofilm formation and testing with several antibiotics in vitro. Gentamicin and rifampicin are the most effective inhibitors of Staph. aureus biofilm-related infections. We recommend MBEC™-HTP assay for rapid testing of multiple substances and TMZF(®) and CrCo discs for low-throughput testing of antibiotic susceptibility and for microscopic analysis. SIGNIFICANCE AND IMPACT OF THE STUDY: In vitro assays can improve the understanding of biofilms and help developing methods to eliminate biofilms from implant surfaces. One advantage of the TMZF(®) and CrCo discs as biofilm in vitro assay is that these metals are commonly used for orthopaedic implants. These models are usable for future periprosthetic joint infection studies.
Assuntos
Antibacterianos/farmacologia , Biofilmes , Poliestirenos , Próteses e Implantes/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/fisiologia , Gentamicinas/farmacologia , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Rifampina/farmacologia , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/crescimento & desenvolvimentoRESUMO
In this study, the potential of focus-variation microscopic imaging was evaluated in a study of morphological patterns of the potential medicinal fungus Hericium coralloides (Basidiomycota). We created three-dimensional reconstructions and visualizations using the imaging technique on a fresh H. coralloides basidioma. The aim was to approximate the spore dispersal efficiency of this basidiomata type regarding the investment of tissue biomass and its reproductive output (production of basidiospores). Results were correlated with published data gained from magnetic resonance imaging and micro-computed tomography. It is demonstrated that focus-variation microscopic imaging results in a more distinct picture of the morphology of the edible and potentially medicinal H. coralloides basidiomata. However, a direct measurement of spore production was not possible. Spore production could only be estimated in combination with a mathematical model because the surface was not directly measurable due to the cellular heterogeneity. However, focus-variation microscopic imaging allows a better and faster estimation of spore production compared with the published methods. Furthermore, it was found that a scanning resolution of 5× is sufficient for determining the fungal surface precisely because at a higher resolution artifacts occur, resulting in adulteration of the image.
Assuntos
Agaricales , Basidiomycota , Hericium , Microscopia , Microtomografia por Raio-XRESUMO
In alveolar type II cells, the release of surfactant is considerably delayed after the formation of exocytotic fusion pores, suggesting that content dispersal may be limited by fusion pore diameter and subject to regulation at a postfusion level. To address this issue, we used confocal FRAP and N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl) pyridinium dibromide (FM 1-43), a dye yielding intense localized fluorescence of surfactant when entering the vesicle lumen through the fusion pore (Haller, T., J. Ortmayr, F. Friedrich, H. Volkl, and P. Dietl. 1998. Proc. Natl. Acad. Sci. USA. 95:1579-1584). Thus, we have been able to monitor the dynamics of individual fusion pores up to hours in intact cells, and to calculate pore diameters using a diffusion model derived from Fick's law. After formation, fusion pores were arrested in a state impeding the release of vesicle contents, and expanded at irregular times thereafter. The expansion rate of initial pores and the probability of late expansions were increased by elevation of the cytoplasmic Ca2+ concentration. Consistently, content release correlated with the occurrence of Ca2+ oscillations in ATP-treated cells, and expanded fusion pores were detectable by EM. This study supports a new concept in exocytosis, implicating fusion pores in the regulation of content release for extended periods after initial formation.
Assuntos
Cálcio/fisiologia , Exocitose , Alvéolos Pulmonares/metabolismo , Vesículas Secretórias/ultraestrutura , Trifosfato de Adenosina/farmacologia , Animais , Células Cultivadas , Corantes Fluorescentes/química , Cinética , Fusão de Membrana , Microscopia Confocal , Microscopia Eletrônica de Varredura , Alvéolos Pulmonares/ultraestrutura , Surfactantes Pulmonares/metabolismo , Compostos de Piridínio/química , Compostos de Amônio Quaternário/química , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos TestesRESUMO
BACKGROUND: Osteoporosis is a common metabolic disease, with mesenchymal stem cells discussed to play an important role in its pathomechanism. For in vitro osteoporosis studies, selection of adequate culture conditions is mandatory so as to preserve cell properties as far as possible. A suitable cell culture surface would ideally provide reproducible experimental conditions by resembling those in-vivo. OBJECTIVE: Generating an improved growth surface for osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBMSCs). METHODS: We modified electrospun gelatine meshes with hydroxyapatite nanopowder. The potential beneficial impact of the ensuing culture conditions were evaluated by cultivating and comparing the growth of cells from osteoporotic and non-osteoporotic donors on either hydroxyapatite-gelatine (HA) meshes, pure gelatine meshes, or 2D standard tissue culture surfaces. RESULTS: After 21 days of differentiation, cells grown on pure or HA-gelatine meshes showed significantly higher mineralization levels compared to cells cultured in standard conditions. The amount of mineralization varied considerably in hBMSC cultures of individual patients but showed no significant difference between stem cells obtained from osteoporotic or non-osteoporotic donors. CONCLUSIONS: Overall, these results indicate that the use of HA-gelatine meshes as growth surfaces may serve as a valuable tool for cultivation and differentiation of mesenchymal stem cells along the osteogenic lineage, facilitating future research on osteoporosis and related issues.
Assuntos
Materiais Biocompatíveis/química , Durapatita/química , Gelatina/química , Células-Tronco Mesenquimais/citologia , Osteogênese , Alicerces Teciduais/química , Idoso , Idoso de 80 Anos ou mais , Técnicas de Cultura de Células , Diferenciação Celular , Células Cultivadas , Matriz Extracelular/química , Feminino , Humanos , MasculinoRESUMO
Dendritic cells are leukocytes of bone marrow origin. They are central to the control of the immune response. Dendritic cells are highly specialized in processing and presenting antigens (microbes, proteins) to helper T lymphocytes. Thereby, they critically regulate further downstream processes such as the development of cytotoxic T lymphocytes, the production of antibodies by B lymphocytes, or the activation of macrophages. A new field of dendritic cell biology is the study of their potential role in inducing peripheral tolerance. The immunogenic/tolerogenic potential of dendritic cells is increasingly being utilized in immunotherapy, particularly for the elicitation of antitumor responses. One very important specialization of dendritic cells is their outstanding capacity to migrate from sites of antigen uptake to lymphoid organs. Much has been learned about this process from studying one particular type of dendritic cell, namely, the Langerhans cell of the epidermis. Therefore, the migratory properties of Langerhans cells are reviewed. Knowledge about this "prototype dendritic cell" may help researchers to understand migration of other types of dendritic cells.
Assuntos
Movimento Celular/fisiologia , Células Epidérmicas , Células de Langerhans/fisiologia , Sistema Linfático/fisiologia , Animais , Membrana Basal/metabolismo , Membrana Basal/ultraestrutura , Técnicas de Cultura/métodos , Epiderme/fisiologia , Humanos , Tolerância Imunológica/fisiologia , Infecções/terapia , Células de Langerhans/imunologia , Células de Langerhans/ultraestrutura , Sistema Linfático/imunologia , Sistema Linfático/ultraestrutura , Neoplasias/terapiaRESUMO
Injections of WGA-HRP were made in the rat trigeminal ganglion and C1-3 dorsal root ganglia (DRGs) to study the central projection patterns and their relations to each other. Trigeminal ganglion injections resulted in heavy terminal labeling in all trigeminal sensory nuclei. Prominent labeling was also observed in the solitary tract nucleus and in the medial parts of the dorsal horn at C1-3 levels, but labeling could be followed caudally to the C7 segment. Contralateral trigeminal projections were found in the nucleus caudalis and in the dorsal horn at C1-3 levels. The C1 DRG was found to be inconstant in the rat. When it was present, small amounts of terminal labeling were found in the external cuneate nucleus (ECN) and the central cervical nucleus (CCN). No dorsal horn projections were seen from the C1 DRG. Injections in the C2 DRG resulted in heavy labeling in the ECN, nucleus X, CCN, and dorsal horn, where it was mainly located in lateral areas. Labeling could be followed caudally to the Th 7 segment. C2 DRG projections also appeared in the cuneate nucleus (Cun), in all the trigeminal sensory nuclei, and in the spinal, medial, and lateral vestibular nuclei. A small C2 DRG projection was observed in the ventral cochlear nucleus. C3 DRG injections resulted in heavy labeling in both medial middle and lateral parts of the dorsal horn, in the ECN, and in nucleus X, whereas the labeling in the CCN was somewhat weaker. Smaller projections were seen to trigeminal nuclei, Cun, and the column of Clarke. Comparisons of the central projection fields of trigeminal and upper cervical primary afferents indicated a somatotopic organization but with a certain degree of overlap.
Assuntos
Encéfalo/citologia , Gânglios Espinais/citologia , Neurônios Aferentes/fisiologia , Ratos/anatomia & histologia , Nervo Trigêmeo/citologia , Animais , Encéfalo/fisiologia , Feminino , Gânglios/citologia , Gânglios/fisiologia , Gânglios Espinais/fisiologia , Peroxidase do Rábano Silvestre , Masculino , Pescoço , Ratos Endogâmicos , Nervo Trigêmeo/fisiologia , Aglutininas do Germe de TrigoRESUMO
Injections of WGA-HRP were made in the rat C4-C8 dorsal root ganglia (DRGs) individually to study the central projections and their relations to each other. The main dorsal horn projections from these DRGs to the dorsal horn lamina II extended for about two segments rostrally and caudally to the injected DRG, whereas the projections to laminae I, III, and IV were less restricted rostrocaudally. Comparisons of the dorsal horn projections from the DRGs investigated indicated a tendency for a somatotopic organization, which was most prominent in lamina II. Labeled central branches from the C4-8 DRGs could be traced in the dorsal column as far caudally as 12-17 segments caudal to the level of entrance. Most of these fibers appeared to end in the medial dorsal horn base, including the column of Clarke. Labeling of primary afferents in the ventral horn generally extended for at least 3-4 segments rostral and caudal to the level of the injected DRG. Projections to the central cervical nucleus were most prominent from the C4 DRG and gradually became less prominent from the more caudal DRGs. Heavy projections to the cuneate nucleus (Cun) originated from the C7 and C8 DRG, whereas those from the C4-C6 DRGs were less extensive. The Cun projections from the different DRGs appeared to overlap, and the same was true for the projections to the external cuneate nucleus. Projections to the gracile nucleus, the vestibular nuclear complex, including nucleus X, and to trigeminal sensory nuclei were seen from all DRGs investigated.
Assuntos
Encéfalo/anatomia & histologia , Gânglios Espinais/citologia , Vias Aferentes/anatomia & histologia , Animais , Encéfalo/citologia , Peroxidase do Rábano Silvestre , Masculino , Ratos , Ratos Endogâmicos , Conjugado Aglutinina do Germe de Trigo-Peroxidase do Rábano Silvestre , Aglutininas do Germe de TrigoRESUMO
The innervation of the rat hard palate and the bordering part of the soft palate was studied after anterograde transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) and to choleragenoid (B-HRP) in separate experiments. WGA-HRP labeling showed leakage from several types of nerve endings, whereas B-HRP did not. Both conjugates gave rise to heavy labeling of a variety of nerve endings. Intragemmal and, especially, perigemmal fibers were labeled in chemosensory corpuscles, which were most common in the medial wall of the incisive canal and in the most anterior part of the soft palate. Ruffini endings of different sizes were labeled in the incisive papilla. Other subepithelial endings forming elongated expanded profiles with medium- to large-caliber source fibers were most common in protruding parts of the palate. Labeled intraepithelial endings included Merkel endings, which were most frequent in the incisive papilla and the rugae. Other labeled profiles were medium-caliber afferents giving rise to irregular, beaded, and sometimes branched endings often located far superficially in the epithelium. Such endings were present both within and between protruding parts of the palate. Fine-caliber intraepithelial endings were labeled almost exclusively in WGA-HRP experiments.
Assuntos
Palato/inervação , Animais , Peroxidase do Rábano Silvestre , Microscopia Eletrônica , Terminações Nervosas , Neurônios Aferentes , Palato/fisiologia , Palato Mole/inervação , Palato Mole/fisiologia , Ratos , Ratos Sprague-Dawley , Gânglio Trigeminal , Conjugado Aglutinina do Germe de Trigo-Peroxidase do Rábano Silvestre , Aglutininas do Germe de TrigoRESUMO
The regulation of the vasculature in the skin is a complex process involving both perivascular nerves and local endothelial-mediated control. In this study, the perivascular innervation in the mystacial pad of the rat was characterized based upon immunochemical and lectin binding characteristics and distribution. All of the innervation labeled with anti-protein gene product 9.5 (PGP 9.5), which was used in double- and triple-labeling combinations with the Griffonia simplicifolia lectin (GSA) and antibodies against a variety of neuropeptides, enzymes, and structural proteins. GSA histofluorescence revealed an intricate microvasculature within the rows of tactile vibrissae, which form a natural grid to standardize analyses. Specific features of the vascular organization were confirmed by scanning electron microscopy. Each interval between adjacent vibrissae contained a predictably organized microvascular module composed of separate arterial channels and capillary networks for each of several different structures: papillary muscles, facial muscles, the interior of vibrissal follicle-sinus complexes, vibrissal papillae, and the upper dermis of the intervibrissal fur. Each module was innervated by at least two sets of sensory, at least two sets of sympathetic, and at least one possible set of parasympathetic. These sets not only differed in their biochemical characteristics, but also in their relative position within the arterial walls and their distribution among the microvasculature to the various structures. As such, the microvasculature to each type of structure had a particular combination of innervation, suggesting that separate neuronal mechanisms may be involved in regulating the blood flow to different types of targets even within the confines of a small territory of tissue.
Assuntos
Sistema Nervoso Autônomo/citologia , Microcirculação/inervação , Proteínas do Tecido Nervoso/análise , Neurônios Aferentes/citologia , Neuropeptídeos/análise , Ratos Sprague-Dawley/anatomia & histologia , Pele/irrigação sanguínea , Vibrissas , Animais , Anticorpos , Enzimas/análise , Feminino , Lectinas , Microcirculação/ultraestrutura , Microscopia Eletrônica de Varredura , Modelos Estruturais , Neurônios Aferentes/ultraestrutura , Ratos , Vibrissas/ultraestruturaRESUMO
The peripheral reorganization of pulpal nerves after tooth injury was studied, in the rat, with anterograde horseradish peroxidase tracing techniques, and combined retrograde Fluorogold tracing and immunohistochemistry was employed to examine the effects of inferior alveolar nerve lesions or tooth injury on some cytochemical characteristics of pulpal trigeminal ganglion nerve cells, namely content of substance P, calcitonin gene-related peptide and the ganglioside GM1 (binding subunit of cholera toxin), as well as affinity to RT 97 (antibody to neurofilament protein) and the lectin Griffonia simplicifolia isolectin I-B4. Anterograde horseradish peroxidase tracing demonstrated that pulpal nerves either disappear or reinnervate novel targets after loss of pulpal tissue. There were no obvious signs of neuroma formation. Retrograde Fluorogold labelling with immunohistochemistry showed that after inferior alveolar nerve lesions with subsequent regeneration, a much higher proportion of Fluorogold cells (15%) were substance P-positive compared to normal (2%). In addition, 3% of the cells were Griffonia simplicifolia isolectin I-B4-positive. Such cells were very rare in controls. Proportions of calcitonin gene-related peptide-, GM1- and RT-97-positive cells were normal. After tooth lesions, the proportions of Fluorogold-positive substance P-, Griffonia simplicifolia isolectin I-B4-, GM1- and RT 97-labelled cells were similar to controls, while the proportion of calcitonin gene-related peptide-positive neurons was reduced. The results show that pulpal deafferentation may change the long-term cytochemical characteristics of affected trigeminal ganglion neurons.
Assuntos
Polpa Dentária/inervação , Estilbamidinas , Gânglio Trigeminal/fisiologia , Animais , Corantes Fluorescentes , Peroxidase do Rábano Silvestre , Imuno-Histoquímica , Microscopia de Fluorescência , Ratos , Ratos Endogâmicos , Extração Dentária , Gânglio Trigeminal/citologiaRESUMO
Low and high molecular weight isoforms of the mitogen and multifunctional cytokine basic fibroblast growth factor (FGF-2) are up-regulated in neurons and glial cells in response to peripheral nerve lesion. While synthesis, regulation and functions of FGF-2 in non-neuronal cells are well established, the significance of neuronal FGF-2 remains to be investigated in the peripheral nervous system. Therefore, the expression, intracellular localization and possible effects of FGF-2 isoforms were analyzed in primary sympathetic neurons derived from the rat superior cervical ganglion. FGF-2 is detected in the nucleus and in perinuclear Golgi fields of early postnatal neurons which also express mRNA and protein for the FGF receptor type 1. Biolistic transfection of plasmids encoding FGF-2 isoforms fused to fluorescent proteins demonstrates nuclear targeting of 18 kDa FGF-2 and 23 kDa FGF-2 with prominent accumulation in the nucleolus of neurons. Neither overexpression nor treatment with FGF-2 isoforms promotes survival of sympathetic neurons deprived of nerve growth factor; however, neuronal transfection of the high molecular weight FGF-2 isoform in dissociated and slice cultures results in a bi- or multinuclear phenotype. The present study provides evidence for neuronal synthesis and targeting of FGF-2 to the nucleus and Golgi apparatus supporting a dual role of FGF-2 in the nucleus and secretory pathway of sympathetic neurons.
Assuntos
Compartimento Celular/genética , Diferenciação Celular/fisiologia , Fator 2 de Crescimento de Fibroblastos/genética , Mitose/genética , Neurônios/metabolismo , Gânglio Cervical Superior/metabolismo , Animais , Animais Recém-Nascidos , Compartimento Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Nucléolo Celular/genética , Nucléolo Celular/metabolismo , Nucléolo Celular/ultraestrutura , Núcleo Celular/genética , Núcleo Celular/metabolismo , Núcleo Celular/ultraestrutura , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Células Cultivadas , Fator 2 de Crescimento de Fibroblastos/farmacologia , Complexo de Golgi/genética , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Técnicas In Vitro , Microscopia Eletrônica , Mitose/efeitos dos fármacos , Peso Molecular , Fator de Crescimento Neural/genética , Fator de Crescimento Neural/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/ultraestrutura , Isoformas de Proteínas/genética , RNA Mensageiro/efeitos dos fármacos , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores de Fatores de Crescimento de Fibroblastos/metabolismo , Gânglio Cervical Superior/crescimento & desenvolvimento , Gânglio Cervical Superior/ultraestruturaRESUMO
Ultrastructurally, all cells of human fetal membranes strongly exhibit a large amount of lipid deposits throughout pregnancy. Their origin and function is still unknown. The aim of this study was to investigate the localization of key components of lipid metabolism in this tissue. Using immunohistochemical techniques, the distribution of lipoprotein lipase (LPL), low density lipoprotein receptors (LDL receptors), and apo-lipoprotein B and E was investigated in 20 human fetal membranes at term. In addition, electron microscopy was used to study the intracellular localization of lipoprotein-sized particles. Amnionic epithelium and trophoblast cells reacted strongly for LPL. LDL receptors and apo-lipoproteins were present in amnionic epithelium and fibroblasts of the amnion. In none of the investigated cells were lipoprotein-sized particles identified. Similar results were obtained in all 20 cases. The findings indicate that lipoprotein from the amniotic fluid or from the maternal circulation may serve as substrate for lipids in human fetal membranes.
Assuntos
Âmnio/química , Apolipoproteínas/análise , Córion/química , Lipase Lipoproteica/análise , Receptores de LDL/análise , Trofoblastos/química , Âmnio/citologia , Âmnio/enzimologia , Córion/citologia , Córion/enzimologia , Células Epiteliais/química , Células Epiteliais/enzimologia , Células Epiteliais/ultraestrutura , Feminino , Fibroblastos/química , Fibroblastos/citologia , Fibroblastos/enzimologia , Humanos , Imuno-Histoquímica , Microscopia Eletrônica , Gravidez , Trofoblastos/citologia , Trofoblastos/enzimologiaRESUMO
BACKGROUND: Occlusion of coronary arteries during beating heart surgery bears the potential for mechanical trauma to the arterial wall with consequent endothelial injury. The aim of this study was to elucidate the effects of local occlusion on the beating heart in human coronary arteries. METHODS: Coronary arteries of patients with dilated cardiomyopathy (n = 7) or ischemic heart disease (n = 10) undergoing heart transplantation were locally occluded after starting cardiopulmonary bypass. Immediately after excision of the diseased heart, the vessels were fixed. Unoccluded segments served as controls. Integrity of endothelial lining was observed with scanning electron microscopy. RESULTS: Scanning electron microscopy revealed significantly more severe endothelial injury in the area of occlusion than in the adjacent, not manipulated control segments. In the region of local occlusion, plaque rupture was noted in three of 34 atherosclerotic vessel specimens, injury to side branches was evident in two of 44, and local microthrombus formation was evident in six of 44 samples. CONCLUSIONS: Local occlusion of human coronary arteries during beating heart coronary surgery may cause focal endothelial denudation, local microthrombosis, atherosclerotic plaque rupture, and injury to target vessel side branches.
Assuntos
Ponte Cardiopulmonar/efeitos adversos , Vasos Coronários/lesões , Endotélio Vascular/lesões , Transplante de Coração , Anastomose Cirúrgica/efeitos adversos , Cardiomiopatia Dilatada/cirurgia , Doença das Coronárias/cirurgia , Vasos Coronários/patologia , Endotélio Vascular/patologia , HumanosRESUMO
PURPOSE: To correlate clinical and histological findings after lamellar keratoplasty, phototherapeutic keratectomy, and application of a donor lenticule on a human cornea. METHODS: A cornea was obtained during penetrating keratoplasty. The specimen was fixated, dehydrated and embedded in Epon resin. The tissue was cut in 0.5-microm-thick semi-thin sections, stained with toluidine blue, and studied with light microscopy. RESULTS: The central part of the photoablated cornea, which was covered by the donor lenticule, did not differ from a normal cornea. Peripherally, a hazy ring was found clinically. Histology showed an irregular epithelium. Where it was thickened, the epithelium was hyperplastic and showed an increased number of cell layers. In the hazy region, Bowman's layer was absent, indicating that the donor lenticule did not cover this part of the photokeratectomized cornea. The anterior-most part of the corneal stroma was vacuolized and contained amorphous extracellular material; swollen keratocytes were present in this region. Beneath this layer, collagen lamellae were wavy and interwoven and keratocytes were increased in number, appeared swollen, and some had assumed an atypical shape. Peripheral to the haze, the cornea was clear. Histologically, the epithelium was irregular and hyperplastic, Bowman's layer was absent, and stromal collagen lamellae were abnormally organized, but no vacuolization was found. CONCLUSIONS: The formation of haze after excimer laser photokeratectomy can be minimized if the ablated stroma is covered by a corneal lenticule.
Assuntos
Substância Própria/patologia , Transplante de Córnea/patologia , Epitélio Corneano/patologia , Miopia/patologia , Ceratectomia Fotorrefrativa , Contagem de Células , Tamanho Celular , Substância Própria/cirurgia , Epitélio Corneano/cirurgia , Fibroblastos/patologia , Humanos , Hiperplasia/patologia , Lasers de Excimer , Masculino , Pessoa de Meia-Idade , Miopia/cirurgia , Reoperação , Doadores de TecidosRESUMO
PURPOSE: To report a case of cerebral ischemia confirmed by magnetic resonance imaging after successful cardiopulmonary resuscitation (CPR) complicated by acute respiratory injury. MATERIALS AND METHODS: After 4 min of cardiac arrest, followed by 3 min of basic life support CPR, a female pig weighing 38 kg received every 5 min vasopressin (0.4, 0.4 and 0.8 U/kg). After 22 min of cardiac arrest, including 18 min of CPR, one defibrillation attempt employing 100 J resulted in return of spontaneous circulation. Neurological evaluation was performed 24 and 96 h after successful CPR. Magnetic resonance imaging was carried out 4 days after CPR using a clinical 1.5 T scanner. The magnetic resonance imaging protocol consisted of fast spinecho T2-weighted, as well as spinecho T1-weighted imaging of the brain. RESULTS: CPR with vasopressin resulted in excellent coronary perfusion pressure ranging between 35 and 60 mm Hg throughout CPR. Eight minutes after initiation of chest compressions, bleeding out of the tracheal tube occurred. This was later confirmed as originating from bilateral bloody pulmonary infiltrations, resulting in acute respiratory injury in the post-resuscitation phase. Ninety-six hours after successful CPR, magnetic resonance imaging revealed bilateral diffuse cerebral vasogenic edema. CONCLUSION: Although excellent coronary perfusion pressure renders a return of spontaneous circulation more likely, complications such as acute respiratory injury in the post-resuscitation phase have to be managed carefully in order to ensure good neurological recovery from cardiac arrest.
Assuntos
Reanimação Cardiopulmonar , Circulação Coronária , Sistema Nervoso/fisiopatologia , Doença Aguda , Animais , Encéfalo/patologia , Isquemia Encefálica/diagnóstico , Isquemia Encefálica/fisiopatologia , Feminino , Parada Cardíaca/complicações , Parada Cardíaca/terapia , Hemorragia/etiologia , Intestino Delgado/patologia , Pneumopatias/etiologia , Imageamento por Ressonância Magnética , Miocárdio/patologia , Transtornos Respiratórios/etiologia , Análise de Sobrevida , Suínos , Fatores de Tempo , Falha de TratamentoRESUMO
In this study the detailed morphology and the function of cartilage canals in the chicken femur are investigated. Several embryonic stages (e 13.5, 16, 19, and 20) are examined by means of light microscopy, electron microscopy (TEM), and immunohistochemistry (VEGF, type I and II collagen). Our results show that cartilage canals originate from the perichondrium and form a complex pattern. Two types of canals are distinguishable: shell canals and communicating canals. Shell canals are in the reserve zone and are arranged in successive layers. Communicating canals spring from the shell canals and pass down into the proliferative zone and into the hypertrophic zone. These canals are conical shaped and are orientated nearly in parallel to the long axis of the femur. Cartilage canals comprise venules, arterioles, capillaries (mature and immature), and undifferentiated mesenchymal cells. No canal wall in the sense of an epithelium is elaborated. VEGF is detected in both types of canals and macrophages are found at the end of the cartilage canals. We conclude that the growth factor stimulates angiogenesis and that the latter cells erode the matrix ahead of the canals and thus enable the advancement of the vessels. The results clearly show that the canal matrix differs from the remaining cartilage matrix. The canal matrix contains type I collagen, few type II collagen fibrils and proteoglycans are lacking. In contrast, in the cartilage matrix type II collagen and proteoglycans are abundant but no type I collagen is found. Communicating canals are surrounded by a distinct layer of type I collagen indicating that osteoid is formed around these canals. Hypertrophic chondrocytes label for type I collagen and it seemed possible that chondrocytes adjacent to the communicating canals differentiate into bone-forming cells. Our results provide evidence that cartilage canals are involved in nourishment of the cartilage as well as in the ossification process.
Assuntos
Cartilagem/embriologia , Fêmur/embriologia , Organogênese , Osteogênese/fisiologia , Animais , Calcificação Fisiológica , Cartilagem/metabolismo , Cartilagem/ultraestrutura , Embrião de Galinha , Colágeno Tipo I/metabolismo , Colágeno Tipo II/metabolismo , Matriz Extracelular/metabolismo , Fêmur/metabolismo , Imageamento Tridimensional/métodos , Imuno-Histoquímica , Microscopia Eletrônica de Varredura , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
To test the oxyphoretic properties and potential nephrotoxic side-effects of polymerized hemoglobin solutions, isolated rat kidneys were perfused in a recirculating system for 180 min. Group I was perfused with a substrate enriched Ringer solution containing hydroxyethylstarch (HES) to produce isoncotic conditions. In group II HES was substituted in part by UPPBHb (34 g/l) with a high portion of low molecular weight molecules (= UPPBHb1). In group III 34 g/l of UPPBHb containing an increased fraction of high molecular weight polymers (= UPPBHb2) was used. Only UPPBHb2-perfused kidneys showed a reduced renal perfusate flow (RPF, 13.3 +/- 1.1 ml/min g kw), when compared to HES-perfused controls (15.5 +/- 0.8) and UPPBHb1 (15.1 +/- 1.2). Glomerular filtration rate (GFR) was significantly higher in UPPBHb1-perfused kidneys (902 +/- 107 vs 633 +/- 55 microliters/min g kw for HES). This difference became even more pronounced in the third hour of perfusion (474 +/- 125 vs. 103 +/- 33). In contrast, UPPBHb2 produced low initial GFR levels of 385 +/- 25, which had only a minor tendency to decline with time. Parallel to GFR, absolute reabsorption of sodium (TNa) andoxygen consumption (QO2) showed values of 110 +/- 16 and 5.46 +/- 0.33 mumol/min g kw in UPPBHb1-kidneys vs 83 +/- 6 and 5.09 +/- 0.27 in controls and vs 53 +/- 4 and 3.66 +/- 0.12 in UPPBHb2-kidneys. Fractional excretion of sodium (FENa), of potassium (FEK), and of water (FEH2O) in UPPBHb1 and UPPBHb2-perfused kidneys were not significantly different from HES-perfused controls at any time of perfusion. Urinary flow rate (UFR) was similar in UPPBHb1- and HES-kidneys. Nevertheless, control kidneys tended to render oliguric during the third hour of perfusion (UFR 19.9 +/- 4.1 microliters/min g kw), whereas UPPBHb1 preserved urinary flow in a better way (83.7 +/- 32.4). UFR of UPPBHb2-kidneys was significantly reduced initially (30.2 +/- 5.1 vs. 105 +/- 33 for HES), but increased steadily up to 67 +/- 23. In the UPPBHb1 and HES group, all functional parameters determined declined dramatically within the third hour of perfusion, whereas UPPBHb2 produced functional stability. The in vivo reaction pattern of renal autoregulation was better preserved in UPPBHb-perfused kidneys than in HES-perfused controls: 74 +/- 6 vs. 59 +/- 5 vs. 42 +/- 4% (of full autoregulatory response) for UPPBHb1, UPPBHb2, and HES kidneys, respectively. Light- and electron microscopic analysis revealed major alterations only for the outer medulla of HES-kidneys.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Substitutos Sanguíneos/farmacologia , Hemoglobinas/farmacologia , Rim/fisiologia , Polímeros/farmacologia , Animais , Substitutos Sanguíneos/química , Bovinos , Taxa de Filtração Glomerular , Hemoglobinas/química , Derivados de Hidroxietil Amido/farmacologia , Rim/patologia , Masculino , Perfusão , Polímeros/química , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
Cerebral malaria (CM) is associated with high mortality and morbidity as a certain percentage of survivors suffers from persistent neurological sequelae. The mechanisms leading to death and functional impairments are yet not fully understood. This study investigated biochemical and morphological markers of apoptosis in the brains of mice infected with Plasmodium berghei ANKA. Cleaved caspase-3 was detected in the brains of animals with clinical signs of CM and immunoreactivity directly correlated with the clinical severity of the disease. Caudal parts of the brain showed more intense immunoreactivity for cleaved caspase-3. Double-labelling experiments revealed processing of caspase-3 primarily in neurons and oligodendrocytes. These cells also exhibited apoptotic-like morphological profiles in ultrastructural analysis. Further, cleavage of caspase-3 was found in endothelial cells. In contrast to neurons and oligodendrocytes, apoptosis of endothelial cells already occurred in early stages of the disease. Our results are the first to demonstrate processing of caspase-3 in different central nervous system cells of animals with CM. Apoptosis of endothelial cells may represent a critical issue for the development of the disease in the mouse model. Neurological signs and symptoms might be attributable, at least in part, to apoptotic degeneration of neurons and glia in advanced stages of murine CM.