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1.
Proc Natl Acad Sci U S A ; 111(39): 14106-11, 2014 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-25225359

RESUMO

Recent advances in nanoscale bioreplication processes present the potential for novel basic and applied research into organismal behavioral processes. Insect behavior potentially could be affected by physical features existing at the nanoscale level. We used nano-bioreplicated visual decoys of female emerald ash borer beetles (Agrilus planipennis) to evoke stereotypical mate-finding behavior, whereby males fly to and alight on the decoys as they would on real females. Using an industrially scalable nanomolding process, we replicated and evaluated the importance of two features of the outer cuticular surface of the beetle's wings: structural interference coloration of the elytra by multilayering of the epicuticle and fine-scale surface features consisting of spicules and spines that scatter light into intense strands. Two types of decoys that lacked one or both of these elements were fabricated, one type nano-bioreplicated and the other 3D-printed with no bioreplicated surface nanostructural elements. Both types were colored with green paint. The light-scattering properties of the nano-bioreplicated surfaces were verified by shining a white laser on the decoys in a dark room and projecting the scattering pattern onto a white surface. Regardless of the coloration mechanism, the nano-bioreplicated decoys evoked the complete attraction and landing sequence of Agrilus males. In contrast, males made brief flying approaches toward the decoys without nanostructured features, but diverted away before alighting on them. The nano-bioreplicated decoys were also electroconductive, a feature used on traps such that beetles alighting onto them were stunned, killed, and collected.


Assuntos
Besouros/fisiologia , Comportamento Sexual Animal/fisiologia , Comunicação Animal , Animais , Materiais Biomiméticos , Cor , Feminino , Voo Animal , Masculino , Modelos Biológicos , Nanoestruturas , Nanotecnologia , Comportamento Estereotipado , Visão Ocular
2.
Opt Lett ; 39(7): 2125-8, 2014 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-24686691

RESUMO

The Dyakonov-Tamm wave was experimentally excited along the periodically corrugated interface of magnesium fluoride as the isotropic homogeneous partnering material and a zinc-selenide chiral sculptured thin film (STF) as the anisotropic and periodically nonhomogeneous partnering material. The total transmittance of a p-polarized 633 nm laser beam was measured as a function of the angle of incidence for several thicknesses of the chiral STF to identify those dips in total transmittance that are indicative of the excitation of a Dyakonov-Tamm wave.

3.
Ann Bot ; 112(6): 1141-8, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23960046

RESUMO

BACKGROUND AND AIMS: Blue-green iridescence in the tropical rainforest understorey sedge Mapania caudata creates structural coloration in its leaves through a novel photonic mechanism. Known structures in plants producing iridescent blues consist of altered cellulose layering within cell walls and in special bodies, and thylakoid membranes in specialized plastids. This study was undertaken in order to determine the origin of leaf iridescence in this plant with particular attention to nano-scale components contributing to this coloration. METHODS: Adaxial walls of leaf epidermal cells were characterized using high-pressure-frozen freeze-substituted specimens, which retain their native dimensions during observations using transmission and scanning microscopy, accompanied by energy-dispersive X-ray spectroscopy to identify the role of biogenic silica in wall-based iridescence. Biogenic silica was experimentally removed using aqueous Na2CO3 and optical properties were compared using spectral reflectance. KEY RESULTS AND CONCLUSIONS: Blue iridescence is produced in the adaxial epidermal cell wall, which contains helicoid lamellae. The blue iridescence from cell surfaces is left-circularly polarized. The position of the silica granules is entrained by the helicoid microfibrillar layers, and granules accumulate at a uniform position within the helicoids, contributing to the structure that produces the blue iridescence, as part of the unit cell responsible for 2 ° Bragg scatter. Removal of silica from the walls eliminated the blue colour. Addition of silica nanoparticles on existing cellulosic lamellae is a novel mechanism for adding structural colour in organisms.


Assuntos
Celulose/metabolismo , Cyperaceae/química , Dióxido de Silício/química , Parede Celular/química , Parede Celular/metabolismo , Cor , Cyperaceae/metabolismo , Cyperaceae/ultraestrutura , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Nanopartículas/química , Óptica e Fotônica , Epiderme Vegetal/química , Epiderme Vegetal/metabolismo , Epiderme Vegetal/ultraestrutura , Folhas de Planta/química , Folhas de Planta/metabolismo , Folhas de Planta/ultraestrutura
4.
J Forensic Sci ; 60(1): 179-85, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25284136

RESUMO

A fingermark on a nonporous substrate can be developed by depositing a columnar thin film (CTF) on it, but the CTF technique's sensitivity for low-quality fingermarks is unknown. The optimized CTF and traditional development of several depletion series of sebaceous-loaded fingermarks were compared using a split-print methodology as well as subjective and objective grading schemes, in a limited laboratory trial. CTF development was superior to development with selected traditional techniques on brass, anodized aluminum, black acrylonitrile butadiene styrene (ABS), and white nylon. On white ABS and black nylon, the CTF technique performed poorly but still as well as the best-performing traditional development technique. The CTF technique was more sensitive on brass and anodized aluminum than, and as sensitive on the four hard plastics and stainless steel as, the best-performing traditional technique. Thus, the CTF technique is useful to develop friction-ridge detail from limited fingermark residue on some smooth substrates.

5.
J Forensic Sci ; 60(2): 295-302, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25421147

RESUMO

Both the columnar-thin-film (CTF) and the vacuum-metal-deposition (VMD) techniques for visualizing sebaceous fingermarks require the deposition of a material thereon in a vacuum chamber. Despite that similarity, there are many differences between the two techniques. The film deposited with the CTF technique has a columnar morphology, but the film deposited with the VMD technique comprises discrete islands. A split-print methodology on a variety of fingermarked substrates was used to determine that the CTF technique is superior for developing fingermarks on clear sandwich bags and partial bloody fingermarks on stainless steel. Both techniques are similar in their ability to develop fingermarks on glass but the CTF technique yields higher contrast. The VMD technique is superior for developing fingermarks on white grocery bags and the smooth side of Gloss Finish Scotch Multitask(™) tape. Neither technique worked well for fingermarks on black garbage bags.


Assuntos
Dermatoglifia , Medicina Legal/métodos , Sangue , Humanos , Microscopia Eletrônica de Varredura , Propriedades de Superfície , Vácuo , Volatilização
6.
J Forensic Sci ; 59(1): 94-102, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24400828

RESUMO

A form of physical vapor deposition, called the conformal-evaporated-film-by-rotation (CEFR) method, was optimized for the conformal deposition of columnar thin films (CTFs) on sebaceous fingermarks. Relying on the surface topology of the fingermark, the CTF development technique is different from traditional development techniques. After the optimization of the development conditions, the CTF development technique was found to be superior to traditional development methods on several nonporous substrates: the smooth side of Scotch(®) Multitask, Gorilla(®) , and Scotch(®) Duct tapes; clear and black soft plastics; stained and sealed walnut and cherry woods; partial bloody fingermarks on stainless steel; and discharged cartridge casings. It was equally as good as other development techniques on other substrates, but worse on a few. The optimization study is expected to assist in designing a mobile CEFR apparatus capable of on-scene development of fingermarks.


Assuntos
Dermatoglifia , Nanoestruturas , Sebo , Cianoacrilatos , Fluorescência , Humanos , Pós , Glândulas Sebáceas , Propriedades de Superfície , Volatilização
7.
Sci Rep ; 3: 1409, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23474988

RESUMO

The commonly used optical sensor based on surface plasmon-polariton wave phenomenon can sense just one chemical, because only one SPP wave can be guided by the interface of a metal and a dielectric material contained in the sensor. Multiple analytes could be detected and/or the sensing reliability for a single analyte could be enhanced, if multiple SPP-wave modes could be excited on a single metal/dielectric interface. For that to happen, the partnering dielectric material must be periodically non-homogeneous. Using a chiral sculptured thin film (CSTF) as that material in a SPP-wave platform, we show that the angular locations of multiple SPP-wave modes shift when the void regions of the CSTF are infiltrated with a fluid. The sensitivities realized in the proof-of-concept experiments are comparable to state-of-research values.

8.
Forensic Sci Int ; 231(1-3): 204-7, 2013 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-23890638

RESUMO

The grading of fingerprint quality by fingerprint examiners as currently practised is a subjective process. Therefore, an objective system was devised to remove the subjectivity. The devised grading system is quantitative and uses three separate, easily available, software packages to ultimately identify the portions of a fingerprint that correspond to low-, medium-, and high-quality definitive minutiae as defined on the Universal Latent Workstation of the US Federal Bureau of Investigation.


Assuntos
Dermatoglifia , Processamento de Imagem Assistida por Computador/métodos , Software , Algoritmos , Cianoacrilatos , Humanos , Volatilização
9.
J Forensic Sci ; 56(3): 612-6, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21306377

RESUMO

Various vacuum techniques are employed to develop fingermarks on evidentiary items. In this work, a vacuum was used to deposit columnar thin films (CTFs) on untreated, cyanoacrylate-fumed or dusted fingermarks on a limited selection of nonporous surfaces (microscope glass slides and evidence tape). CTF deposition was not attempted on fingermarks deposited on porous surfaces. The fingermarks were placed in a vacuum chamber with the fingermark side facing an evaporating source boat containing either chalcogenide glass or MgF(2). Thermal evaporation of chalcogenide glass or MgF(2) under a 1 µTorr vacuum for 30 min formed dense CTFs on fingermark ridges, capturing the topographical features. The results show that it is possible to capture fingermark topology using CTFs on selected untreated, vacuumed cyanoacrylate-fumed or black powder-dusted nonporous surfaces. Additionally, the results suggested this might be a mechanism to help elucidate the sequence of deposition.


Assuntos
Dermatoglifia , Cianoacrilatos , Vidro , Humanos , Microscopia Eletrônica de Varredura , Pós , Vácuo , Volatilização , Difração de Raios X/métodos
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