The dynamics of B-cell reconstitution post allogeneic hematopoietic stem cell transplantation: A real-world study.

BACKGROUND: The immune reconstitution after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is crucial for preventing infections and relapse and enhancing graft-versus-tumor effects. B cells play an important role in humoral immunity and immune regulation, but their reconstitution after allo-HSCT has not been well studied. METHODS: In this study, we analyzed the dynamics of B cells in 252 patients who underwent allo-HSCT for 2 years and assessed the impact of factors on B-cell reconstitution and their correlations with survival outcomes, as well as the development stages of B cells in the bone marrow and the subsets in the peripheral blood. RESULTS: We found that the B-cell reconstitution in the bone marrow was consistent with the peripheral blood (p = 0.232). B-cell reconstitution was delayed by the male gender, age >50, older donor age, the occurrence of chronic and acute graft-versus-host disease, and the infections of fungi and cytomegalovirus. The survival analysis revealed that patients with lower B cells had higher risks of death and relapse. More importantly, we used propensity score matching to obtain the conclusion that post-1-year B-cell reconstitution is better in females. Meanwhile, using mediation analysis, we proposed the age-B cells-survival axis and found that B-cell reconstitution at month 12 posttransplant mediated the effect of age on patient survival (p = 0.013). We also found that younger patients showed more immature B cells in the bone marrow after transplantation (p = 0.037). CONCLUSION: Our findings provide valuable insights for optimizing the management of B-cell reconstitution and improving the efficacy and safety of allo-HSCT.

Evolutionary Implications of the RNA N6-Methyladenosine Methylome in Plants.

Epigenetic modifications play important roles in genome evolution and innovation. However, most analyses have focused on the evolutionary role of DNA modifications, and little is understood about the influence of posttranscriptional RNA modifications on genome evolution. To explore the evolutionary significance of RNA modifications, we generated transcriptome-wide profiles of N6-methyladenosine (m6A), the most prevalent internal modification of mRNA, for 13 representative plant species spanning over half a billion years of evolution. These data reveal the evolutionary conservation and divergence of m6A methylomes in plants, uncover the preference of m6A modifications on ancient orthologous genes, and demonstrate less m6A divergence between orthologous gene pairs with earlier evolutionary origins. Further investigation revealed that the evolutionary divergence of m6A modifications is related to sequence variation between homologs from whole-genome duplication and gene family expansion from local-genome duplication. Unexpectedly, a significant negative correlation was found between the retention ratio of m6A modifications and the number of family members. Moreover, the divergence of m6A modifications is accompanied by variation in the expression level and translation efficiency of duplicated genes from whole- and local-genome duplication. Our work reveals new insights into evolutionary patterns of m6A methylomes in plant species and their implications, and provides a resource of plant m6A profiles for further studies of m6A regulation and function in an evolutionary context.

Exploring transcriptional switches from pairwise, temporal and population RNA-Seq data using deepTS.

Transcriptional switch (TS) is a widely observed phenomenon caused by changes in the relative expression of transcripts from the same gene, in spatial, temporal or other dimensions. TS has been associated with human diseases, plant development and stress responses. Its investigation is often hampered by a lack of suitable tools allowing comprehensive and flexible TS analysis for high-throughput RNA sequencing (RNA-Seq) data. Here, we present deepTS, a user-friendly web-based implementation that enables a fully interactive, multifunctional identification, visualization and analysis of TS events for large-scale RNA-Seq datasets from pairwise, temporal and population experiments. deepTS offers rich functionality to streamline RNA-Seq-based TS analysis for both model and non-model organisms and for those with or without reference transcriptome. The presented case studies highlight the capabilities of deepTS and demonstrate its potential for the transcriptome-wide TS analysis of pairwise, temporal and population RNA-Seq data. We believe deepTS will help research groups, regardless of their informatics expertise, perform accessible, reproducible and collaborative TS analyses of large-scale RNA-Seq data.

Evolution of the RNA N 6-Methyladenosine Methylome Mediated by Genomic Duplication.

RNA N 6-methyladenosine (m6A) modification is the most abundant form of RNA epigenetic modification in eukaryotes. Given that m6A evolution is associated with the selective constraints of nucleotide sequences in mammalian genomes, we hypothesize that m6A evolution can be linked, at least in part, to genomic duplication events in complex polyploid plant genomes. To test this hypothesis, we presented the maize (Zea mays) m6A modification landscape in a transcriptome-wide manner and identified 11,968 m6A peaks carried by 5,893 and 3,811 genes from two subgenomes (maize1 and maize2, respectively). Each of these subgenomes covered over 2,200 duplicate genes. Within these duplicate genes, those carrying m6A peaks exhibited significant differences in retention rate. This biased subgenome fractionation of m6A-methylated genes is associated with multiple sequence features and is influenced by asymmetric evolutionary rates. We also characterized the coevolutionary patterns of m6A-methylated genes and transposable elements, which can be mediated by whole genome duplication and tandem duplication. We revealed the evolutionary conservation and divergence of duplicated m6A functional factors and the potential role of m6A modification in maize responses to drought stress. This study highlights complex interplays between m6A modification and gene duplication, providing a reference for understanding the mechanisms underlying m6A evolution mediated by genome duplication events.

DLG1-AS1 is activated by MYC and drives the proliferation and migration of hepatocellular carcinoma cells through miR-497-5p/SSRP1 axis.

BACKGROUND: Long non-coding RNAs (lncRNAs) have been reported to be biological regulators in hepatocellular carcinoma (HCC). DLG1 antisense RNA 1 (DLG1-AS1) has been found to be up-regulated in cervical cancer. However, its function and underlying mechanism in HCC remains unknown. METHODS: DLG1-AS1 expression was assessed in HCC cells and normal cell by RT-qPCR. Luciferase reporter assay, RNA pull down assay and RIP assay were used to demonstrate the interaction between DLG1-AS1 and miR-497-5p. RESULTS: DLG1-AS1 was highly expressed in HCC cells. Silencing of DLG1-AS1 led to the inhibition of HCC cell growth and migration. Besides, MYC induced the transcriptional activation of DLG1-AS1. MYC could facilitate HCC cellular processes by up-regulating DLG1-AS1. MiR-497-5p could interact with DLG1-AS1 in HCC cells. Down-regulation of miR-497-5p could reverse the impacts of DLG1-AS1 silencing on HCC cells. SSRP1 expression could be positively regulated by DLG1-AS1 but was negatively regulated by miR-497-5p. Knockdown of DLG1-AS1 suppressed tumor growth in nude mice. CONCLUSIONS: DLG1-AS1 is activated by MYC and functions as an oncogene in HCC via miR-497-5p/SSRP1 axis.

Stereotactic Biopsy for Brainstem Lesions: A Meta-analysis with Noncomparative Binary Data.

OBJECTIVES: To evaluate the diagnostic yield and safety of brainstem stereotactic biopsy for brainstem lesions. METHODS: We performed a meta-analysis of English articles retrieved from the PubMed, Web of Science, Cochrane Library, and APA psycInfo databases up to May 12, 2021. A binary fixed-effect model, the inverse variance method, or a binary random-effect model, the Dersimonian Laird method, were utilized for pooling the data. This meta-analysis was registered with INPLASY, INPLASY202190034. FINDINGS: A total of 41 eligible studies with 2792 participants were included. The weighted average diagnostic yield was 97.0% (95% confidential interval [CI], 96.0-97.9%). The weighted average proportions of temporary complications, permanent deficits, and deaths were 6.2% (95% CI, 4.5-7.9%), .5% (95% CI, .2-.8%), and .3% (95% CI, .1-.5%), respectively. The subgroup analysis indicated a nearly identical weighted average diagnostic yield between MRI-guided stereotactic biopsy and CT-guided stereotactic biopsy (95.9% vs 95.8%) but slightly increased proportions of temporary complications (7.9% vs 6.0%), permanent deficits (1.9% vs .2%), and deaths (1.1% vs .4%) in the former compared to the latter. Moreover, a greater weighted average diagnostic yield (99.2% vs 97.6%) and lower proportions of temporary complications (5.1% vs 6.8%) and deaths (.7% vs 1.5%) were shown in the pediatric patient population than in the adult patient population. CONCLUSIONS: Brainstem stereotactic biopsy demonstrates striking accuracy plus satisfying safety in the diagnosis of brainstem lesions. The diagnostic yield, morbidity, and mortality mildly vary based on the diversity of assistant techniques and subject populations.

Prognostic significance of XIAP and NF-κB expression in esophageal carcinoma with postoperative radiotherapy.

BACKGROUND: X-chromosome-linked IAP (XIAP) and nuclear factor-κB (NF-κB) are frequently overexpressed and correlate closely with chemoradiotherapy resistance and poor prognosis in many cancers. However, the significance of XIAP and NF-κB expression in radiotherapy sensitivity and its effect on the prognosis of esophageal squamous cell carcinoma (ESCC) are still unknown. The aim of this study was to examine XIAP and NF-κB status in ESCC patients undergoing postoperative radiotherapy after radical surgery, and to evaluate their clinical significance. METHODS: A total of 78 ESCC patients treated with postoperative radiotherapy after radical surgery were enrolled in this study. We immunohistochemically investigated the expression of XIAP and NF-κB in tissues from enrolled patients with specific antibodies. Then, the correlations among XIAP, NF-κB expression, clinicopathological features and its prognostic relevance in ESCC were analyzed. RESULTS: The increased expression of XIAP and NF-κB in ESCC tissues were clearly correlated with the tumor differentiation and p-TNM stage. Significant positive correlations were found between the expression status of XIAP and NF-κB (r = 0.779, P = 0.000). Overexpression of XIAP and NF-κB and metastasis were significantly associated with shorter overall survival times in univariate analysis (P < 0.05). Multivariate analysis also confirmed that XIAP expression was an independent prognostic factor (P = 0.005). CONCLUSIONS: XIAP and NF-κB are intensively expressed in ESCC. The level of XIAP is positively correlated to progression and prognosis of ESCC.

Influence of Light Conditions on the Antibacterial Performance and Mechanism of Waterborne Fluorescent Coatings Based on Waterproof Long Afterglow Phosphors/PDMS Composites.

Marine microbial adhesion is the fundamental cause of large-scale biological fouling. Low surface energy coatings can prevent marine installations from biofouling; nevertheless, their static antifouling abilities are limited in the absence of shear forces produced by seawater. Novel waterborne antifouling coatings inspired by fluorescent coral were reported in this paper. Waterproof long afterglow phosphors (WLAP) were introduced into waterborne silicone elastomers by the physical blending method. The composite coatings store energy during the day, and the various colors of light emitted at night affect the regular physiological activities of marine bacteria. Due to the synergistic effect of fouling-release and fluorescence antifouling, the WLAP/polydimethylsiloxane (PDMS) composite coating showed excellent antifouling abilities. The antibacterial performance of coatings was tested under simulated day-night alternation, continuous light, and constant dark conditions, respectively. The results illustrated that the antibacterial performance of composite coatings under simulated day-night alternation conditions was significantly better than that under continuous light or darkness. The weak lights emitted by the coating can effectively inhibit the adhesion of bacteria. C-SB/PDMS showed the best antibacterial effect, with a bacterial adhesion rate (BAR) of only 3.7%. Constant strong light also affects the normal physiological behavior of bacteria, and the weak light of coatings was covered. The antibacterial ability of coatings primarily relied on their surface properties under continuous dark conditions. The fluorescent effect played a vital role in the synergetic antifouling mechanism. This study enhanced the static antifouling abilities of coatings and provided a new direction for environmentally friendly and long-acting marine antifouling coatings.

The Effect of Conductive Polyaniline on the Anti-Fouling and Electromagnetic Properties of Polydimethylsiloxane Coatings.

In this paper, four conductive polyaniline powders doped in hydrochloric acid, sulfuric acid, phosphoric acid, and sulfonic acid were selected and blended with polydimethylsiloxane to prepare coatings with an electromagnetic absorption effect and fouling desorption effect, respectively. A UV spectrophotometer was used to evaluate the settling rate of the powders. Fourier transform infrared spectrometry, laser confocal microscopy, and scanning electron microscopy were used to observe the morphology and structure of the powder and the coating. The interface properties of the coatings were characterized using a contact angle measurement, the mechanical properties of the coatings using a tensile test, and the electromagnetic properties of the powders and microwave absorption properties of the coatings using vector network analyzers. Meanwhile, the antifouling performance of the coatings was evaluated via the marine bacteria adhesion test and benthic diatom adhesion test, and the effect of conductive polyaniline on the antifouling performance of the coating was analyzed. The results show that adding polyaniline reduced the surface energy of the coating and increased the roughness, mechanical properties and anti-fouling properties of the coating. Moreover, adding appropriate polyaniline powder can enhance the electromagnetic wave loss of the coating. The followings values were recorded for a hydrochloric-acid-doped polyaniline coating: lowest surface energy of 17.17 mJ/m2, maximum fracture strength of 0.95 MPa, maximum elongation of 155%, maximum bandwidth of 3.81 GHz, and peak of reflection loss of -23.15 dB. The bacterial detachment rate of the polydimethylsiloxane (PDMS) samples was only 30.37%. The bacterial adhesion rates of the composite coating containing hydrochloric-acid-doped polyaniline were 4.95% and 2.72% after rinsing and washing, respectively, and the desorption rate was 45.35%. The chlorophyll concentration values were 0.0057 mg/L and 0.0028 mg/L, respectively, and the desorption rate was 54.62%.

Anti-marine biofouling adhesion performance and mechanism of PDMS fouling-release coating containing PS-PEG hydrogel.

Polystyrene microspheres compounded with polyethylene glycol-based hydrogel (PS-PEG)/polydimethylsiloxane (PDMS) coatings were prepared using the physical blending method. The chemical structure, surface and interface properties, interlayer adhesion, and tensile properties were tested in this paper. Furthermore, the antifouling performance was evaluated through bovine serum albumin fluorescent protein adsorption testing, marine bacteria adhesion testing, and benthic diatom adhesion testing. The results showed that the coating performance was best when 20 wt% PS-PEG hydrogel was added. Its surface energy was only 19.21 mJ/m2, the maximum breaking strength was 1.24 MPa, the maximum elongation rate was 675 %, the elastic modulus was 2.53 MPa, and the anti-stripping rate was 100 %. In addition, the coating with added 20 wt% PS-PEG hydrogel bacterial adherence rate was 5.36 % and 2.45 % after rinsing and washing, respectively, and the removal rate was 54.29 %. In the benthic diatom adhesion test, the chlorophyll concentration a-value was only 0.0017 mg/L after washing with added 20 wt% hydrogel, and the protein desorption rate was 84.19 % higher than PDMS in the fluorescent protein adsorption test. This coating has the 'low adhesion' and 'desorption' characteristics in the three growth stages of biofouling. Meanwhile, the low surface energy of the silicone is stable, and the hydrogel also dynamically migrates to the surface to gradually form a hydration layer, both are synergistic. When 20 wt% PS-PEG hydrogel was added, the coating demonstrated excellent antifouling performance due to its high hydration layer, low surface energy, high elasticity, and high interlayer adhesion. This research is expected to contribute to the practical applications of hydrogel coatings in marine antifouling.

DNA repair pathways-targeted cyclovirobuxine inhibits castration-resistant prostate cancer growth by promoting cell apoptosis and cycle arrest.

BACKGROUND: Castration-resistant prostate cancer (CRPC) is a deadly malignancy without effective therapeutics. Cyclovirobuxine (CVB) can play an anticancer role by inhibiting mitochondrial function, regulating tumor cell apoptosis, dysregulating autophagy, and other mechanisms. This study aimed to examine the function and mechanism of CVB in CRPC to provide new insights into CRPC treatment. METHODS: The effect of CVB on PC3 and C4-2 cell viability was determined using a CCK8 assay. Core therapeutic targets of CVB in CRPC cells were identified using RNA sequencing, online database, and PPI network analyses. Western blotting, RT-qPCR and molecular docking were performed to evaluate the regulation of core targets by CVB. Utilizing GO and KEGG enrichment analyses, the probable anti-CRPC mechanism of CVB was investigated. Immunofluorescence, flow cytometry and colony formation assays were used to verify the potential phenotypic regulatory role of CVB in CRPC. RESULTS: CVB inhibited CRPC cell activity in a concentration-dependent manner. Mechanistically, it primarily regulated BRCA1-, POLD1-, BLM-, MSH2-, MSH6- and PCNA-mediated mismatch repair, homologous recombination repair, base excision repair, Fanconi anemia repair, and nucleotide excision repair pathways. Immunofluorescence, Western blot, flow cytometry and colony formation experiments showed that CVB induced DNA damage accumulation, cell apoptosis, and cell cycle arrest and inhibited CRPC cell proliferation. CONCLUSION: CVB can induce DNA damage accumulation in CRPC cells by targeting DNA repair pathways and then induce cell apoptosis and cell cycle arrest, eventually leading to inhibition of the long-term proliferation of CRPC cells.

Swelling Behaviour of Polystyrene Microsphere Enhanced PEG-Based Hydrogels in Seawater and Evolution Mechanism of Their Three-Dimensional Network Microstructure.

To understand the microstructure evolution of hydrogels swollen in seawater, freeze-drying technology was used to fix and preserve the swollen three-dimensional microstructure. By this method, we revealed the swelling behavior of hydrogels in seawater, and elucidated the mechanism of the swelling process. Meanwhile, we also used Fourier-transform infrared spectroscopy; laser confocal microscopy; field emission scanning electron microscopy, and swelling performance tests to research the structure and properties of PS-PEG hydrogels, before and after seawater swelling, and analyzed the structure and properties of PEG-based hydrogels with different contents of polystyrene microspheres. Results showed that PS-PEG hydrogels went through three stages during the swelling process, namely 'wetting-rapid swelling-swelling equilibrium'. Due to the capillary effect and hydration effect, the surface area would initially grow tiny pores, and enter the interior in a free penetration manner. Finally, it formed a stable structure, and this process varied with different content of polystyrene microspheres. In addition, with the increase of polystyrene microsphere content, the roughness of the hydrogel before swelling would increase, but decrease after swelling. Appropriate acquisition of polystyrene microspheres could enhance the three-dimensional network structure of PEG-based hydrogels, with a lower swelling degree than hydrogels without polystyrene microspheres.

Preparation of g-C3N4/TNTs/CNTs Photocatalytic Composite Powder and Its Enhancement of Antifouling Performance of Polydimethylsiloxane Coatings.

Semiconductor photocatalytic materials have shown potential in the field of antifouling due to their good antibacterial properties, stability, and nontoxic properties. It is an effective way to use them to improve the static antifouling performance of silicone antifouling coatings. g-C3N4/TNTs/CNTs (CNTC) photocatalytic composite powders were prepared and introduced into polydimethylsiloxane (PDMS) coatings to enhance their antifouling performance. Firstly, g-C3N4/TNTs with heterostructure were thermally polymerized by urea and TiO2 nanotubes (TNTs), and then g-C3N4/TNTs and multi-walled carbon nanotubes (CNTs) were composited to obtain CNTC. Finally, CNTC was added into PDMS to prepare g-C3N4/TNTs/CNTs/PDMS (CNTC/P) composite antifouling coating. The results showed that CNTC successfully recombined and formed a heterostructure, and the recombination rate of photogenerated carriers decreased after recombination. The addition of CNTC to PDMS increased the hydrophobicity and roughness while reducing the surface energy (SE) of the coatings. CNTC could effectively improve the anti-attachment performance of PDMS coatings to bacteria and benthic diatom. The bacterial attachment rate (AB) and benthic diatom attachment rate (AD) of CNTC/P-20 were, respectively, 13.1% and 63.1%; they are much lower than that of the coating without photocatalytic composite powder. This coating design provides a new idea for developing new "efficient" and "green" photocatalytic composite antifouling coatings.

SLAP@g-C3N4 Fluorescent Photocatalytic Composite Powders Enhance the Anti-Bacteria Adhesion Performance and Mechanism of Polydimethylsiloxane Coatings.

Fluorescent antifouling and photocatalytic antifouling technologies have shown potential in the field of marine antifouling. SLAP@g-C3N4/PDMS (SLAP@CN/PDMS) composite antifouling coatings were designed and prepared using g-C3N4, sky-blue long afterglow phosphor (SLAP), and polydimethylsiloxane (PDMS). The fluorescence emitted by SLAP under dark conditions was used to excite g-C3N4 for fluorescent photocatalysis and to prolong the photocatalytic activity of g-C3N4. Key data were collected by testing and characterization and are presented in this work. The results showed that g-C3N4 was successfully coated on the SLAP surface and formed a heterogeneous structure. After the composite powder was added to the PDMS coating, the coating maintained low surface energy but enhanced the surface roughness of the coating. The experimental results of degraded Rhodamine B (RhB) showed that SLAP prolonged the g-C3N4 photocatalytic activity time. The anti-marine bacterial adhesion performance of the coating was investigated by bacterial adhesion experiments. The results showed that SLAP@CN could effectively improve the anti-bacterial adhesion performance of PDMS coating, in which the anti-bacterial adhesion performance of SLAP@CN-2.5/PDMS was improved by nearly 19 times. This antifouling coating introduces fluorescent antifouling, photocatalytic antifouling, and fluorescence-driven photocatalytic antifouling based on the low surface energy antifouling of silicones and achieves "all-weather" fluorescent photocatalytic antifouling.

Preparation and Properties of PED-TDI Polyurethane-Modified Silicone Coatings.

To explore the influence mechanisms of polyurethane soft segments on modified silicone coatings, a series of modified coatings was prepared by introducing different contents of hydroxypropyl-terminated polydimethylsiloxane (PDMS2200) into the soft segment of polyurethane. ATR-FTIR, NMR, CLSM, AFM, contact angle measurement, the tensile test, bacterial adhesion, and the benthic diatom adhesion test were used to investigate the structure, morphology, roughness, degree of microphase separation, surface energy, tensile properties, and antifouling properties of the modified coatings. The results show that PDMS2200 could aggravate the microphase separation of the modified coatings, increase the surface-free energy, and reduce its elastic modulus; when the microphase separation exceeded a certain degree, increasing PDMS2200 would decrease the tensile properties. The PED-TDI polyurethane-modified silicone coating prepared with the formula of PU-Si17 had the best tensile properties and antifouling properties among all modified coatings.

Effect of Microwave-Assisted Curing on Properties of Waterborne Silicone Antifouling Coatings.

Waterborne silicone coatings are prepared in this paper by using silicone emulsion as a film-forming material, γ-methacryloxypropyltrimethoxysilane, and dibutyltin dilaurate as a curing agent and a catalyst, respectively. The corresponding coatings are obtained by controlling different microwave times to accelerate the coating curing. The surface morphology, roughness, surface properties, mechanical properties, and antifouling properties of the coating are studied by laser confocal microscope, contact angle measurement, tensile test, marine bacterial attachment test, and benthic diatom adhesion test. Additionally, the action mechanism of microwaves in the curing process of the coatings is also discussed. The results show that the microwave can greatly reduce the curing time of waterborne silicone coating. It can improve the painting efficiency, the surface roughness of the coating, and the mechanical properties of the coatings. The change in roughness increases the contact angle of the coating, reduces the apparent surface energy, and then improves the antifouling performance. For the coating cured by microwave, with the increase in microwave curing time, the water and diiodomethane contact angles of the coating gradually increase, and the surface energy gradually decreases from about 20 mJ/m2 to 10.8 mJ/m2. With the increase in microwave time, the attachment amount of Navicular Tenera gradually decreases, the removal rate gradually increases, and the removal rate of Navicular Tenera in the coating increases from 15.36% to 31.78%. The bacterial removal rate of the coating can be increases from 11.05% to 22.28% after microwave curing. Microwave-assisted curing is helpful in improving the antifouling and self-cleaning performance of waterborne silicone coatings, showing promising potential applications.

Preparation and Properties of Fluorosilicone Fouling-Release Coatings.

To improve the antifouling performance of silicone fouling-release coatings, some fluorosilicone and silicone fouling-release coatings were prepared and cured at room temperature with hydroxyl-terminated fluoropolysiloxane (FPS) or hydroxy-terminated polydimethylsiloxane (PDMS) as a film-forming resin, tetraethyl orthosilicate (TEOS) as a crosslinking agent, and dibutyltin dilaurate (DBTDL) as a catalyst. The chemical structure, surface morphology and roughness, tensile properties, and antifouling properties of the coating were studied by infrared spectroscopy, a laser confocal scanning microscope, contact angle measurement, tensile tests, and marine bacteria and benthic diatom attachment tests. The results showed that the FPS coatings were not only hydrophobic but also oleophobic, and the contact angles of the FPS coatings were larger than those of the PDMS coatings. The surface free energies of the FPS coatings were much lower than those of the PDMS coatings. Generally, the fluorine groups can improve the antifouling performance of the coating. Introducing nonreactive silicone oil into PDMS or FPS coatings can improve the antifouling performance of the coating to a certain extent. The prepared fluorosilicone fouling-release coatings showed good application prospects.

The abnormal expression of chromosomal region maintenance 1 (CRM1)-survivin axis in ovarian cancer and its related mechanisms regulating proliferation and apoptosis of ovarian cancer cells.

Ovarian cancer (OC) is the main type of cancer that affects the female reproductive system and has a high morbidity and mortality rate. This study aimed to explore the regulatory effect of the chromosomal region maintenance 1 (CRM1)-survivin axis on the progression of OC. Ovarian cancer cells were transfected with pcDNA3.1-survivin and short hairpin RNA (sh)-CRM1. Cell proliferation was analyzed by cell counting kit-8 (CCK8), 5-ethynyl-2´-deoxyuridine (EdU) staining, and colony formation assays. Apoptosis was detected using flow cytometry. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were performed to analyze the expression of RNA and protein, respectively. qRT-PCR and prognostic correlation analyses revealed that CRM1 is highly expressed in OC cells and related to survival. The results of qRT-PCR, CCK8, colony formation test, EdU staining, flow cytometry, and Western blotting showed that CRM1 silencing inhibited the proliferation and colony formation of OVCAR 3 and SKOV3 cells and promoted cell apoptosis by promoting Caspase-3 activation. Survivin was positively regulated by CRM1 and promoted the development of OC. The results of the rescue experiment showed that overexpression of survivin reversed the inhibitory effect of CRM1 knockdown on the proliferation of ovarian cancer cells and its inhibitory effect on apoptosis. Our findings confirm the role of the CRM1-survivin signal transduction axis in OC by regulating the proliferation and apoptosis of OC cells, and may thus serve as a potential therapeutic target for OC.

easyMF: A Web Platform for Matrix Factorization-Based Gene Discovery from Large-scale Transcriptome Data.

With the development of high-throughput experimental technologies, large-scale RNA sequencing (RNA-Seq) data have been and continue to be produced, but have led to challenges in extracting relevant biological knowledge hidden in the produced high-dimensional gene expression matrices. Here, we develop easyMF ( https://github.com/cma2015/easyMF ), a web platform that can facilitate functional gene discovery from large-scale transcriptome data using matrix factorization (MF) algorithms. Compared with existing MF-based software packages, easyMF exhibits several promising features, such as greater functionality, flexibility and ease of use. The easyMF platform is equipped using the Big-Data-supported Galaxy system with user-friendly graphic user interfaces, allowing users with little programming experience to streamline transcriptome analysis from raw reads to gene expression, carry out multiple-scenario MF analysis, and perform multiple-way MF-based gene discovery. easyMF is also powered with the advanced packing technology to enhance ease of use under different operating systems and computational environments. We illustrated the application of easyMF for seed gene discovery from temporal, spatial, and integrated RNA-Seq datasets of maize (Zea mays L.), resulting in the identification of 3,167 seed stage-specific, 1,849 seed compartment-specific, and 774 seed-specific genes, respectively. The present results also indicated that easyMF can prioritize seed-related genes with superior prediction performance over the state-of-art network-based gene prioritization system MaizeNet. As a modular, containerized and open-source platform, easyMF can be further customized to satisfy users' specific demands of functional gene discovery and deployed as a web service for broad applications.

Gene expression changes in the pituitary gland of rats exposed to electromagnetic pulses.

OBJECTIVE: We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP). METHODS: The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis. We then validated and further investigated the reduced expression of two tumorigenesis-related genes, Pten, and Jund, by assessing their mRNA and protein expression by quantitative real-time-PCR, western blotting, and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP. RESULTS: EMP exposure induced genome-wide gene expression changes in the rat pituitary gland. There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals. CONCLUSION: EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland. These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.