RESUMO
A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-M53T, was isolated from the insect Acrida cinerea. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain SCU-M53T belonged to the genus Paracoccus, having Paracoccus chinensisNBRC 104937T (97.04 % 16S rRNA gene sequence similarity) and Paracoccus niistensis KCTC 22789T (96.70 %) as the most closely related phylogenetic neighbours. Growth occurred at 10-40 °C (optimum 25-30 °C), pH 5.0-10.0 (optimum pH 6.5-7.5) and with 0-4 % (w/v) NaCl (optimum 0-1 % NaCl). The fatty acids of strain SCU-M53T were C18 : 1ω7c, C18 : 0, C16 : 0, C14 : 0 3-OH, C14 : 0 and C10 : 0 3-OH. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown aminolipid, two unknown phospholipids and two unknown lipids. The isoprenoid quinone was ubiquinone-10. The DNA G+C content was 60.6 mol%. Strain SCU-M53T exhibited 36.5 and 28.6 % DNA-DNA relatedness to P. chinensis NBRC 104937T and P. niistensis KCTC 22789T, respectively. According to these results, strain SCU-M53T represents a novel species of the genus Paracoccus, for which the name Paracoccus acridae sp. nov. is proposed. The type strain is SCU-M53T (=KCTC 42932T=CGMCC 1.15419T).
Assuntos
Ortópteros/microbiologia , Paracoccus/classificação , Filogenia , Animais , Técnicas de Tipagem Bacteriana , Composição de Bases , China , Produtos Agrícolas , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Paracoccus/genética , Paracoccus/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
All locust epiphytic bacteria were screened and a total of 62 epiphytic bacteria were obtained from samples of Acrida cinerea. Via phylogenetic analysis, the 62 epiphytic bacteria were allocated to 27 genera, 18 families, 13 orders, six classes, and four phylums. Then, cyhalothrin degradation experiments were conducted, and the 10 strains that degraded more than 30% cyhalothrin and Paracoccus acridae SCU-M53 showed the highest cyhalothrin degradation rate of 70.5%. Furthermore, Paracoccus acridae SCU-M53 was selected for optimal cyhalothrin biodegradation conditions via the response surface method (Design-Expert). Under the optimum conditions (28 °C, 75 mg/L, and 180 rpm), the cyhalothrin degradation rate reached 79.84% after 2 days. This suggests the possibility that isolating biodegradation cyhalothrin strains from Acrida cinerea is feasible.