RESUMO
Obesity is a disorder of energy balance. Hormone-sensitive lipase (HSL) mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin (encoded by the gene Plin), an adipocyte protein, has been postulated to modulate HSL activity. We show here that targeted disruption of Plin results in healthy mice that have constitutively activated fat-cell HSL. Plin -/- mice consume more food than control mice, but have normal body weight. They are much leaner and more muscular than controls, have 62% smaller white adipocytes, show elevated basal lipolysis that is resistant to beta-adrenergic agonist stimulation, and are cold-sensitive except when fed. They are also resistant to diet-induced obesity. Breeding the Plin -/- alleles into Leprdb/db mice reverses the obesity by ncreasing the metabolic rate of the mice. Our results demonstrate a role for perilipin in reining in basal HSL activity and regulating lipolysis and energy balance; thus, agents that inactivate perilipin may prove useful as anti-obesity medications.
Assuntos
Obesidade/genética , Fosfoproteínas/genética , Fosfoproteínas/fisiologia , Magreza/genética , Tecido Adiposo/patologia , Tecido Adiposo Marrom/patologia , Animais , Proteínas de Transporte , Metabolismo Energético , Lipólise , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Obesidade/patologia , Obesidade/fisiopatologia , Consumo de Oxigênio , Perilipina-1 , Fenótipo , Fosfoproteínas/deficiência , Esterol Esterase/metabolismo , Magreza/patologia , Magreza/fisiopatologiaRESUMO
In rat brain dynamic susceptibility contrast magnetic resonance (MR) images, vessels visible on the same scan plane as the brain tissue were used to measure the characteristics of the input function of the MR contrast agent gadopentetate dimeglumine. MR images were acquired 30 and 60 minutes after intravenous injections of 3 mg/kg and 15 mg/kg NG-Nitro-L-arginine methyl ester (L-NAME) (n = 9). The time of arrival (TOA) and the mean transit time corrected for TOA of the input function were increased by 3 mg/kg or 15 mg/kg L-NAME. The area of the input function was increased by 15 mg/kg L-NAME. In two animals, similar modifications of the input function induced by 20 mg/kg L-NAME were reversed by infusion of sodium nitroprusside. In two other animals, MABP was increased by phenylephrine to a similar extent as in L-NAME experiments, but did not induce the same modifications of the input function, showing that the action of L-NAME on the input function was not simply caused by an effect on MABP. These results show that the input function can be significantly altered by manipulations widely used in cerebrovascular studies. These input function changes have important implications for calculation of cerebral blood flow.
Assuntos
Circulação Cerebrovascular/efeitos dos fármacos , Meios de Contraste/farmacocinética , Inibidores Enzimáticos/farmacologia , Imageamento por Ressonância Magnética/métodos , Meglumina/farmacocinética , NG-Nitroarginina Metil Éster/farmacologia , Compostos Organometálicos/farmacocinética , Ácido Pentético/análogos & derivados , Animais , Combinação de Medicamentos , Gadolínio DTPA , Técnicas de Diluição do Indicador , Masculino , Modelos Teóricos , Nitroprussiato/farmacologia , Ácido Pentético/farmacocinética , Fenilefrina/farmacologia , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Vasoconstritores/farmacologia , Vasodilatadores/farmacologiaRESUMO
Magnetic resonance imaging (MRI) techniques were used to determine the effect of preexisting hyperglycemia on the extent of cerebral ischemia/reperfusion injury and the level of cerebral perfusion. Middle cerebral artery occlusion (MCAO) was induced by a suture insertion technique. Forty one rats were divided into hyperglycemic and normoglycemic groups with either 4 hours of continuous MCAO or 2 hours of MCAO followed by 2 hours of reperfusion. Diffusion-weighted imaging (DWI) was performed at 4 hours after MCAO to quantify the degree of injury in 6 brain regions. Relative cerebral blood flow (CBF) and cerebral blood volume (CBV) were estimated using gradient echo (GE) bolus tracking and steady-state spin echo (SE) imaging techniques, respectively. Brain injury correlated with the perfusion level measured in both SE CBV and dynamic GE CBF images. In the temporary MCAO model, mean lesion size in DWI was 118% larger and hemispheric CBV was reduced by 37% in hyperglycemic compared with normoglycemic rats. Hyperglycemia did not significantly exacerbate brain injury or CBV deficit in permanent MCAO models. We conclude that preexisting hyperglycemia increases acute postischemic MRI-measurable brain cellular injury in proportion to an associated increased microvascular ischemia.
Assuntos
Isquemia Encefálica/fisiopatologia , Hiperglicemia/fisiopatologia , Animais , Isquemia Encefálica/complicações , Hiperglicemia/complicações , Imageamento por Ressonância Magnética , Masculino , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/fisiopatologiaRESUMO
Preexisting hyperglycemia is associated with enhanced reperfusion injury in the postischemic rat brain. The goal of this study was to evaluate whether the hyperglycemic exacerbation of brain injury is associated with enhanced generation of hydroxyl radicals in rats subjected to middle cerebral artery occlusion (2 h), followed by reperfusion (2 h). Magnetic resonance images revealed the exacerbation of focal brain injury in hyperglycemic rats. The salicylate trapping method was used in conjunction with microdialysis to continuously estimate hydroxyl radical production by measurement of the stable adducts 2,3- and 2,5-dihydroxybenzoic acid (DHBA) during ischemia/reperfusion. In normoglycemic rats, from a mean baseline level of 130 nmol/l, 2,3-DHBA levels surged to peak levels of 194 nmol/l 45 min into ischemia and to 197 nmol/l 15-30 min into the reperfusion period, returning to baseline by 2 h into reperfusion. A similar temporal profile was observed in hyperglycemic rats, except that absolute 2,3-DHBA levels were higher (165 nmol/l at baseline, 317 nmol/l peak during ischemia, 333 nmol/l peak during reperfusion), and levels remained significantly high (p < .05) throughout the reperfusion period. These results suggest that hydroxyl radical is an important contributor to the exacerbation of neuronal and cerebrovascular injury after focal ischemia/reperfusion in hyperglycemic rats.
Assuntos
Arteriopatias Oclusivas/metabolismo , Artérias Cerebrais/metabolismo , Gentisatos , Hiperglicemia/metabolismo , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Arteriopatias Oclusivas/complicações , Infarto Cerebral/patologia , Inibidores Enzimáticos/farmacologia , Hidroxibenzoatos , Radical Hidroxila , Hiperglicemia/complicações , Técnicas In Vitro , Imageamento por Ressonância Magnética , Masculino , Microdiálise , NG-Nitroarginina Metil Éster/farmacologia , Óxido Nítrico Sintase/antagonistas & inibidores , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/complicações , Traumatismo por Reperfusão/metabolismoRESUMO
OBJECTIVE: To develop a method to measure wound surface area. DESIGN: Validity study. SETTING: Inpatient service. PARTICIPANTS: Four patients with decubitus ulcers. MEASUREMENTS: A new imaging process estimating wound surface area and volume by NMR spectroscopy of a mold of the pressure ulcer and a comparison measurement of volume of the mold by water displacement. Measurements made serially totalled 17. RESULTS: Measurement of the volume of the mold by the computer system correlated strongly with measurement of the volume by water displacement. Surface area of the pressure sore mold correlated strongly with volume of the mold raised to the two-thirds power. CONCLUSIONS: It is possible for the first time to measure surface area of decubitus ulcers. This may provide a way of determining accurately the dose of newly proposed topical treatments.
Assuntos
Processamento de Imagem Assistida por Computador , Úlcera por Pressão/patologia , Coloides , Humanos , Espectroscopia de Ressonância Magnética , Compostos Orgânicos , Reprodutibilidade dos TestesRESUMO
Microgravity causes rapid decrement in musculoskeletal mass is associated with a marked decrease in circulatory testosterone levels, as we reported in hindlimb-suspended (HLS) rats. In this model which simulates microgravity, we hypothesized that testosterone supplementation should prevent these losses, and we tested this in two studies. Muscle volumes and bone masses were quantitated by using magnetic resonance imaging (MRI) on day 12. In the first study, 12-wk-old Sprague-Dawley rats that were HLS for 12 days lost 28.5% of muscle volume (53.3 +/- 4.8 vs. 74.5 +/- 3.6 cm3 in the ground control rats; P < 0.001) and had a 5% decrease in bone mineral density (BMD) (P < 0.05). In the second study, 30 male 12-wk-old Wistar rats were HLS and were administered either a vehicle (control), testosterone, or nandrolone decanoate (ND). An additional 20 rats were used as ground controls, one-half of which received testosterone. HLS rats had a significant reduction in muscle volume (42.9 +/- 3.0 vs. 56 +/- 1.8 cm3 in ground control rats; P < 0.01). Both testosterone and ND treatments prevented this muscle loss (51.5 +/- 2 and 51.6 +/- 1.2 cm3, respectively; a 63% improvement; P < 0. 05). There were no statistical differences between the two active treatment groups nor with the ground controls. Similarly, there was an 85% improvement in BMD in the testosterone group (1.15 +/- 0.04 vs. 1.04 +/- 0.04 density units in vehicle controls; P < 0.05) and a 76% improvement in the ND group (1.13 +/- 0.07 density units), whereas ground control rats had a BMD of 1.17 +/- 0.03 density units. Because serum testosterone levels are markedly reduced in this model of simulated microgravity, androgen replacement seems to be a rational countermeasure to prevent microgravity-induced musculoskeletal losses.
Assuntos
Androgênios/farmacologia , Osso e Ossos/fisiologia , Elevação dos Membros Posteriores/fisiologia , Músculo Esquelético/fisiologia , Ausência de Peso/efeitos adversos , Anabolizantes/farmacologia , Animais , Peso Corporal/fisiologia , Osso e Ossos/anatomia & histologia , Osso e Ossos/efeitos dos fármacos , Imageamento por Ressonância Magnética , Masculino , Músculo Esquelético/anatomia & histologia , Músculo Esquelético/efeitos dos fármacos , Nandrolona/análogos & derivados , Nandrolona/farmacologia , Decanoato de Nandrolona , Ratos , Ratos Sprague-DawleyRESUMO
The purpose of this study was to investigate the mechanisms by which a nitric oxide synthase (NOS) inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), is neuroprotective in the hyperglycemic rat model of 2 h of transient middle cerebral artery occlusion followed by 2 h of reperfusion (MCAO/R). The salicylate trapping method was used in conjunction with a microdialysis technique to continuously estimate hydroxyl radical (.OH) formation by measurement of the stable adducts 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Extracellular excitatory amino acids (EAAs) were detected from the same microdialysis samples. Magnetic resonance imaging (MRI) techniques were used to measure neuronal and cerebrovascular injury. The magnitude of EAA release correlated with the levels of the .OH adducts. Treatment with L-NAME (3 mg/kg, i.p.) 1 min before MCAO, and again 1 min before reperfusion, reduced the levels of DHBA by 46. 4% and glutamate by 50.5% in the hyperglycemic rats compared to untreated hyperglycemic controls. MRI indicated that L-NAME reduced the no-reflow zone and the cytotoxic lesion volume to 22.5% and 21. 0%, respectively, that of hyperglycemic controls. Co-treatment with the nitric oxide (NO) donor L-arginine completely eliminated the protective effects of l-NAME with respect to .OH and EAA levels as well as MRI lesion volume. Our data suggest that hyperglycemic MCAO/R results in excessive glutamate excitotoxicity, leading to enhanced generation of .OH via a NO-mediated mechanism, in turn resulting in severe ischemia/reperfusion brain injury.
Assuntos
Inibidores Enzimáticos/uso terapêutico , Hiperglicemia/metabolismo , Ataque Isquêmico Transitório/tratamento farmacológico , NG-Nitroarginina Metil Éster/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Óxido Nítrico Sintase/antagonistas & inibidores , Animais , Sobrevivência Celular/efeitos dos fármacos , Citrulina/metabolismo , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Ácido Glutâmico/metabolismo , Radical Hidroxila , Hiperglicemia/complicações , Ataque Isquêmico Transitório/complicações , Ataque Isquêmico Transitório/metabolismo , Imageamento por Ressonância Magnética , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
We tested the hypothesis that the exacerbation of post-ischemic brain tissue injury associated with hyperglycemia in rats is due to toxic metabolism of nitric oxide. We used magnetic resonance imaging (MRI) techniques to measure neuronal and cerebrovascular injury in a 2-h transient focal cerebral ischemia model in normoglycemic and hyperglycemic rats at 3 and 24 h post-ischemia onset. We determined the effect of low dose (3 mg/kg i.p.) treatment with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). Compared to normoglycemia, preexisting hyperglycemia increased the volume of brain tissue exhibiting hyperintensity in diffusion weighted MRI (DWI) by factors of 5.6 and 6.2 at 3 h and 24 h post-ischemia, respectively. A similar increase in tissue volumes exhibiting hyperintense signal in T2-weighted MRI (T2WI) (3.3-fold and 5.6-fold) was observed. Cerebral blood volume MRI indicated a large focal no-reflow zone in hyperglycemic rats. Treatment with L-NAME eliminated the no-reflow zone in the hyperglycemic rats, and reduced tissue volumes of DWI hyperintensity by 86% and 93% at 3 h and 24 h, respectively. Similarly, tissue volumes of T2WI hyperintensity were reduced by 80% and 94% at 3 h and 24 h, respectively. Thus, nitric oxide is an important mediator in the exacerbation of post-ischemic brain injury in hyperglycemic rats. Inhibition of nitric oxide synthase limits edema formation, improves perfusion and reduces infarct volume.
Assuntos
Arginina/análogos & derivados , Hiperglicemia/fisiopatologia , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico/biossíntese , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Arginina/farmacologia , Hiperglicemia/complicações , Imageamento por Ressonância Magnética , Masculino , Monitorização Fisiológica , NG-Nitroarginina Metil Éster , Prosencéfalo/irrigação sanguínea , Prosencéfalo/fisiopatologia , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/complicações , Coloração e Rotulagem , Sais de Tetrazólio , Fatores de TempoRESUMO
Diffusion-weighted magnetic resonance imaging (DWI) with calculation of the apparent diffusion coefficient (ADC) of water is a widely used noninvasive method to measure movement of water from the extracellular to the intracellular compartment during cerebral ischemia. Lamotrigine, a neuronal Na(+) channel blocker, has been shown to attenuate the increase in extracellular concentrations of excitatory amino acids (EAA) during ischemia and to improve neurological and histological outcome. Because of its proven ability to reduce EAA levels during ischemia, lamotrigine should also minimize excitotoxic-induced increases in intracellular water content and therefore attenuate changes in the ADC. In this study, we sought to determine the effect of lamotrigine on intra- and extracellular water shifts during transient global cerebral ischemia. Fifteen New Zealand white rabbits were anesthetized and randomized to one of three groups: a control group, a lamotrigine-treated group, or a sham group. After being positioned in the bore of the magnet, a 12-min 50-s period of global cerebral ischemia was induced by inflating a neck tourniquet. During ischemia and early reperfusion there was a similar and significant decrease of the ADC in both the lamotrigine and control group. The ADC in the sham ischemia group remained at baseline throughout the experiment. Lamotrigine-mediated blockade of voltage-gated sodium channels did not prevent the intracellular movement of water during 12 min 50 s of global ischemia, as measured by the ADC, suggesting that the ADC decline may not be mediated by voltage-gated sodium influx and glutamate release.
Assuntos
Água Corporal/fisiologia , Encéfalo/fisiopatologia , Ataque Isquêmico Transitório/fisiopatologia , Triazinas/farmacologia , Animais , Pressão Sanguínea , Temperatura Corporal , Água Corporal/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Encéfalo/fisiologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Aminoácidos Excitatórios/metabolismo , Lamotrigina , Imageamento por Ressonância Magnética , Coelhos , Reperfusão , Bloqueadores dos Canais de Sódio , Fatores de TempoRESUMO
Canavan disease (CD) is an autosomal recessive disorder caused by aspartoacylase deficiency leading to accumulation of N-acetylaspartic acid and spongy degeneration of the brain. The mouse model for CD showed low levels of glutamate and gamma-aminobutyric acid (GABA) in the brain. Whether the low levels of glutamate and GABA observed in the CD mouse brain lead to abnormal production of glutamate-GABA associated enzymes and resulting succinate production is not obvious. While glutamate dehydrogenase and alpha-ketoglutarate dehydrogenase complex activities are lower in the cerebellum and brain stem of the CD mouse, alanine aminotransferase and succinate semialdehyde dehydrogenase (SSADH) activities and succinate level are similar to the levels observed in the wild type. Deficiency of SSADH has been suggested to be associated with mental retardation and hypotonia, similar to the clinical features of CD. The normal SSADH activity in the CD mouse brain suggests that mental retardation and hypotonia seen in the CD mouse is not due to SSADH activity and if documented also in patients with CD.
Assuntos
Aldeído Oxirredutases/deficiência , Doença de Canavan/enzimologia , Deficiência Intelectual/enzimologia , Hipotonia Muscular/enzimologia , Aldeído Oxirredutases/genética , Animais , Doença de Canavan/genética , Deficiência Intelectual/genética , Camundongos , Camundongos Knockout , Hipotonia Muscular/genética , Succinato-Semialdeído DesidrogenaseRESUMO
Perturbation of the cerebral circulation by occlusion of the vertebral arteries and a carotid artery can be visualized by using MR imaging and the intravascular contrast agent Gd-DTPA complexed to albumin. This tracer consistently reduced the T1 relaxation time in the brain and blood. The difference between hemispheres was revealed by less T1 reduction in the occluded hemisphere and by an adjustment in the display contrast of images that revealed the territory of decreased perfusion. These results were confirmed by comparing them with cerebral blood flow using radioactive microspheres and the intravascular blood volume tracer 51Cr-EDTA. This method, combined with high-resolution MR imaging, can be applied to serial noninvasive studies of cerebral blood volume in ischemia and other conditions.
Assuntos
Volume Sanguíneo , Isquemia Encefálica/fisiopatologia , Circulação Cerebrovascular , Imageamento por Ressonância Magnética , Animais , Barreira Hematoencefálica , Permeabilidade Capilar , Artérias Cerebrais/fisiopatologia , Meios de Contraste , Gadolínio DTPA , Masculino , Compostos Organometálicos , Ácido Pentético , Ratos , Ratos Endogâmicos , Albumina SéricaRESUMO
Events associated with an evolving cerebral infarction were studied using multiple magnetic resonance imaging (MRI) techniques at 4.7 T in a rat model of middle cerebral artery occlusion. High resolution perfusion images revealed a core of absent perfusion surrounded by a zone of slow, but measurable perfusion. Only the core of severest perfusion deficit demonstrated restricted water diffusion as early as 1 hr, consistent with "cytotoxic" cellular edema in the most vulnerable region. Within 24 hours, the area of restricted diffusion encompassed the entire region destined to become infarcted. In spin-echo images, hypointensity, likely reflecting deoxygenated hemoglobin, was visible in the ischemic hemisphere. Edema accumulated over 72 hr primarily in the surrounding slowly perfused rim, consistent with the concept of "vasogenic" edema. These studies demonstrate that multimodal MRI can visualize events which define the ischemic penumbra--deoxygenation, maintenance of transmembrane ionic gradients, reduced flow, and delayed cell death. These experiments noninvasively visualized differential hemodynamic and biochemical processes within the core and perifocal penumbra and will allow quantitation over time of the relationship between blood flow, cytotoxicity and edema in stroke.
Assuntos
Encéfalo/patologia , Infarto Cerebral/diagnóstico , Imageamento por Ressonância Magnética/métodos , Doença Aguda , Animais , Edema Encefálico/diagnóstico , Isquemia Encefálica/diagnóstico , Circulação Cerebrovascular/fisiologia , Meios de Contraste , Dextranos , Óxido Ferroso-Férrico , Processamento de Imagem Assistida por Computador , Ferro , Nanopartículas de Magnetita , Masculino , Óxidos , Ratos , Ratos Sprague-Dawley , Fatores de TempoAssuntos
Soluções para Preservação de Órgãos , Preservação de Órgãos/métodos , Transplante de Pâncreas/métodos , Adenosina , Adolescente , Adulto , Alopurinol , Amilases/sangue , Edema/diagnóstico , Feminino , Glutationa , Humanos , Insulina , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Pancreatopatias/diagnóstico , Rafinose , Fatores de Tempo , Doadores de TecidosAssuntos
Soluções para Preservação de Órgãos , Preservação de Órgãos/métodos , Transplante de Pâncreas , Pâncreas/metabolismo , Adenosina , Adolescente , Adulto , Alopurinol , Edema/etiologia , Glutationa , Humanos , Técnicas In Vitro , Insulina , Imageamento por Ressonância Magnética , Preservação de Órgãos/efeitos adversos , Transplante de Pâncreas/efeitos adversos , Transplante de Pâncreas/fisiologia , Pancreatopatias/etiologia , Rafinose , Fatores de Tempo , Doadores de Tecidos , Água/metabolismoRESUMO
The early evolution of ischemic brain injury under normoglycemic and streptozotocin-induced hyperglycemic plasma conditions was studied using magnetic resonance imaging (MRI). Male Sprague-Dawley rats were subjected to either permanent middle cerebral artery occlusion (MCAO), or 1-h MCAO followed by reperfusion using the intraluminal suture insertion method. The animals were divided into four groups each with eight rats: normoglycemia with permanent MCAO, normoglycemia with 1-h MCAO, hyperglycemia with permanent MCAO, and hyperglycemia with 1-h MCAO. Diffusion-weighted images (DWIs) and T2-weighted images (T2WIs) were aquired every 1 h from 20 min until 6 h after MCAO, at which time cerebral plasma volume images (PVIs) were acquired. Tissue infarction was determined by triphenyltetrazolium chloride staining at 7 h after MCAO. The ischemic damage, measured as the area of DWI and T2WI hyperintensity and tissue infarction, increased significantly in hyperglycemic rats in both permanent and transient MCAO models. In the permanent MCAO model, the maximal apparent water diffusion coefficient (ADC) decline under either normo- or hyperglycemia was about 40%, but the speed of ADC drop was faster in hyperlgycemic rats than in normoglycemic rats. Reperfusion after 1 h of MCAO in normoglycemic rats partly reversed the decline in ADC, whereas the low ADC area continued to expand after reperfusion in the hyperglycemic group. Between the two hyperglycemic groups with either permanent MCAO or reperfusion, no significant difference was found in the infarct volume measured at 7 h after MCAO. However, reperfusion dramatically increased the extent and accelerated the development rate of vasogenic edema. ADC in the hyperglycemic reperfusion group also dropped to a lower level. A large "no-reflow" zone was found in the ischemic hemisphere in the hyperglycemic reperfusion group. This study provides strong evidence to support that preischemic hyperglycemia exacerbates ischemic damage in both transient and permanent MCAO models and demonstrates, using MRI, that reperfusion under preischemic hyperglycemia accelerates the evolution of early ischemic injury.
Assuntos
Isquemia Encefálica/patologia , Hiperglicemia/patologia , Animais , Pressão Sanguínea/fisiologia , Isquemia Encefálica/fisiopatologia , Núcleo Caudado/patologia , Modelos Animais de Doenças , Imageamento por Ressonância Magnética , Masculino , Putamen/patologia , Ratos , Ratos Sprague-DawleyRESUMO
This article describes a novel application of contrast-enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt-labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575-579, 2001.
Assuntos
Cobalto , Imageamento por Ressonância Magnética/métodos , Microscopia/métodos , Neurônios/citologia , Animais , Encéfalo/citologia , Modelos Animais , Moluscos , Vias Neurais/citologiaRESUMO
A limited midline myelotomy at T10 can relieve pelvic cancer pain in patients. This observation is explainable in light of strong evidence in support of the existence of a visceral pain pathway that ascends in the dorsal column (DC) of the spinal cord. In rats and monkeys, responses of neurons in the ventral posterolateral thalamic nucleus to noxious colorectal distention are dramatically reduced after a lesion of the DC at T10, but not by interruption of the spinothalamic tract. Blockade of transmission of visceral nociceptive signals through the rat sacral cord by microdialysis administration of morphine or 6-cyano-7-nitroquinoxaline-2,3-dione shows that postsynaptic DC neurons in the sacral cord transmit visceral nociceptive signals to the gracile nucleus. Retrograde tracing studies in rats demonstrate a concentration of postsynaptic DC neurons in the central gray matter of the L6-S1 spinal segments, and anterograde tracing studies show that labeled axons ascend from this region to the gracile nucleus. A similar projection from the midthoracic spinal cord ends in the gracile and cuneate nuclei. Behavioral experiments demonstrate that DC lesions reduce the nocifensive responses produced by noxious stimulation of the pancreas and duodenum, as well as the electrophysiological responses of ventral posterolateral neurons to these stimuli. Repeated regional blood volume measurements were made in the thalamus and other brain structures in anesthetized monkeys in response to colorectal distention by functional MRI. Sham surgery did not reduce the regional blood volume changes, whereas the changes were eliminated by a DC lesion at T10.
Assuntos
Encéfalo/fisiopatologia , Dor/fisiopatologia , Medula Espinal/fisiopatologia , Vísceras/inervação , Vias Aferentes/fisiologia , Vias Aferentes/fisiopatologia , Animais , Encéfalo/irrigação sanguínea , Circulação Cerebrovascular , Haplorrinos , Humanos , Pelve , Ratos , Medula Espinal/fisiologia , Tálamo/irrigação sanguínea , Tálamo/fisiopatologiaRESUMO
OBJECTIVE: To determine regional cerebral water content in vivo by magnetic resonance imaging (MRI) after the administration of 7.5% saline in brain-lesioned rabbits. DESIGN: Randomized, controlled, intervention trial. SETTING: University animal laboratory. SUBJECTS: Eighteen male New Zealand white rabbits, randomly assigned to one of three groups. INTERVENTIONS: The animals were anesthetized (1% halothane), intubated, and mechanically ventilated to maintain end-tidal CO2 tension between 30 and 35 mm Hg (4 and 4.7 kPa). Arterial and central venous catheters were inserted and arterial blood samples were serially obtained during the experiment. Serum osmolality was measured. A cryogenic cerebral lesion was produced by pouring liquid nitrogen for 1 min into a funnel placed on the intact skull over the right hemisphere. One group of animals received 20 mL of 7.5% saline intravenously 150 mins after the cerebral lesion was generated (7.5% saline group, n = 7). A second group of animals received the same volume of 0.9% saline intravenously (0.9% saline group, n = 7). In a third group of animals (control group, n = 4) no lesion was created and no fluid administered. MEASUREMENTS AND MAIN RESULTS: Five spin-echo T2-weighted MRIs of the brain were acquired at 90 mins (Baseline 1), 120 mins (Baseline 2), 150 mins (Infusion), 180 mins (Infusion + 30 mins), and 210 mins (Infusion + 60 mins) after the generation of the cerebral lesion. In the control group, two scans separated by a time interval of 120 mins were performed. The percent changes in signal intensity between the first and the four following scans of a coronal slice of the central region were determined. Analysis of variance and the Mann-Whitney U test were used for statistical analysis. Data are presented as mean +/- SD; p < .05 was considered significant. Serum osmolality increased significantly from 308 +/- 13 mosm/L to 349 +/- 19 mosm/L after the infusion of 20 mL of 7.5% saline, but did not change after the administration of 0.9% saline. Signal intensity in the area between the caudal edge of the core of the lesion and the basal ganglia was 9 +/- 8% higher on the injured side than in the corresponding area on the contralateral side (p < .05). Compared with Baseline 1, signal intensity at Infusion + 60 mins decreased by 26.3 +/- 13.7% in the 7.5% saline group, whereas it decreased by 10.4 +/- 8.6% in the 0.9% saline group (p < .05 between groups). Signal intensity decreased only slightly and nonsignificantly by 0.6 +/- 4.4% between the two scans in the control group. CONCLUSIONS: The administration of a 7.5% saline solution causes a prompt and substantial decrease in cerebral water content as assessed by spin-echo T2-weighted MRI. Magnetic resonance imaging offers the opportunity for repeated, noninvasive in vivo determinations of cerebral water content.
Assuntos
Água Corporal/metabolismo , Edema Encefálico/metabolismo , Encéfalo/metabolismo , Imageamento por Ressonância Magnética , Solução Salina Hipertônica/administração & dosagem , Animais , Encéfalo/patologia , Edema Encefálico/etiologia , Edema Encefálico/patologia , Modelos Animais de Doenças , Infusões Intravenosas , Masculino , Tamanho do Órgão , Concentração Osmolar , Coelhos , Distribuição AleatóriaRESUMO
We have applied a superparamagnetic iron oxide formulation (AMI-25, Advanced Magnetics, Inc., Cambridge, MA) to image the cerebral vasculature. Contrast-enhanced images of normal anesthetized rats demonstrated excellent gray/white matter differentiation, consistent with known differences in blood perfusion, and cerebrospinal fluid spaces were clearly seen. Alterations in normal perfusion patterns due to barbiturate anesthesia and ischemia were clearly visible. We propose the use of this agent as an adjunct to MRI for the imaging of conditions with perfusion abnormalities.
Assuntos
Encéfalo/anatomia & histologia , Circulação Cerebrovascular , Meios de Contraste , Compostos Férricos , Imageamento por Ressonância Magnética/métodos , Animais , Estudos de Avaliação como Assunto , Masculino , Ratos , Ratos EndogâmicosRESUMO
Ischemia due to middle cerebral artery occlusion was studied in 29 rats from 1 to 24 hours after occlusion using magnetic resonance imaging. Images were made before and after the injection of a superparamagnetic iron oxide compound, AMI-25. Subtraction images demonstrated the region of perfusion deficit as early as 1 hour after occlusion, earlier than conventional T2-weighted images. The area of altered perfusion detected by this technique (subtraction imaging after AMI-25 administration) correlated with that demonstrated by iodoantipyrine autoradiography. Since this magnetic resonance technique can be used to serially estimate the location and size of the ischemic area, the technique can be an important adjunct to metabolic studies of focal ischemia using magnetic resonance spectroscopy. The technique may have clinical applications as well.