RESUMO
In this study, the complete mitochondrial DNA genome sequence of Sinibrama taeniatus was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of S. taeniatus was a circular molecule of 16,623 bp in length. It consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of S. taeniatus was 31.3% A, 26.8% C, 16.1% G, and 25.8% T, with a relatively high A + T content (57.1%). The results could provide useful data for studying genetic diversity and phylogenetics in S. taeniatus and related species.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Composição de Bases , Códon de Iniciação , Códon de Terminação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Variação Genética , Fases de Leitura Aberta/genética , RNA Ribossômico/química , RNA Ribossômico/isolamento & purificação , RNA Ribossômico/metabolismo , RNA de Transferência/química , RNA de Transferência/isolamento & purificação , RNA de Transferência/metabolismo , Análise de Sequência de DNARESUMO
Schizothorax chongi is an endemic and important polyploidy fish in the upper stream of the Yangtze River. S. chongi represents a typical model species to study historical adaptation and evolution in the Tibetan Plateau. In this study, the complete mitochondrial DNA genome sequence of S. chongi was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial DNA (mtDNA) genome sequence of S. chongi is a circular molecule of 16,584 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of S. chongi is 29.6% A, 27.1% C, 17.9% G, and 25.4% T, with a high AT content (55.0%). The results could provide useful data for further studies on phylogenetics, conservation genetics and rational resource management for S. chongi.
Assuntos
Cyprinidae/genética , DNA Mitocondrial/genética , Genoma Mitocondrial/genética , Mitocôndrias/genética , Análise de Sequência de DNA/veterinária , Animais , Composição de Bases/genética , Sequência de Bases , Tamanho do Genoma , RNA Ribossômico/genética , RNA de Transferência/genética , TibetRESUMO
Glyptothorax sinense (Siluriformes, Sisoridae), is a kind of small-sized freshwater fish which mainly distributes in the middle and upper reaches of the Yangtze River in China. In this study, the complete mitochondrial genome of G. sinense was first determined using a PCR-based method. The complete mtDNA sequence is 16,531 bp in length, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop). The overall-based composition was 31.61% A, 26.66% T, 15.38% G and 26.34% C, with a relatively high A + T content (58.27%). This will provide a useful tool for evolutionary and population genetic studies of G. sinense.
Assuntos
Peixes-Gato/genética , DNA Mitocondrial/genética , Genoma Mitocondrial/genética , Mitocôndrias/genética , Análise de Sequência de DNA/veterinária , Animais , Composição de Bases/genética , Sequência de Bases , China , Tamanho do Genoma , Reação em Cadeia da Polimerase , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
Triplophysa anterodorsalis is an endemic fish in the upper stream of the Yangtze River, Jinsha River and its tributaries. However, wild populations of T. anterodorsalis declined sharply due to cascade hydropower stations constructed successively in the Jinsha River during the past decades. In the present study, the complete mitochondrial DNA genome sequence of T. anterodorsalis was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial genome sequence of T. anterodorsalis is a circular molecule of 16,567 bp in size. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of T. anterodorsalis is 27.37% A, 25.68% C, 18.37% G, and 28.57% T, with a relatively a relatively high A + T content (55.94%). The results could provide useful data for studies on genetic structure and diversity and rational resource conservation in T. anterodorsalis.
Assuntos
Cipriniformes/genética , Genoma Mitocondrial/genética , Animais , Composição de Bases/genética , Ordem dos Genes/genética , Tamanho do Genoma/genética , RNA de Transferência/genética , Análise de Sequência de DNA/métodosRESUMO
Lepturichthys fimbriata is an endemic and an important commercial fish which distributes in the upper stream of the Yangtze River. In this study, the complete mitogenome sequence of L. fimbriata has been first sequenced by DNA sequencing based on the PCR fragments. The mitogenome, consisting of 16,567 base pairs (bp), includes 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNA genes and a non-coding control region (CR). The overall base composition of L. fimbriata is 30.4% A, 28.7% C, 16.5% G, and 24.4% T, with a relatively a slight AT bias of 54.7%. CR of 903 bp length is located between tRNA(Pro) and tRNA(Phe). The complete mitochondrial genome sequence would be useful for further studies on conservation genetics and resource management in L. fimbriata.
Assuntos
Peixes/genética , Genes Mitocondriais/fisiologia , Genoma Mitocondrial/fisiologia , Animais , Sequência de Bases , Proteínas de Peixes/genética , Proteínas Mitocondriais/genética , Dados de Sequência Molecular , RNA/genética , RNA Mitocondrial , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
Jinshaia sinensis is an endemic and typical fish which is successfully adaptive to mountain torrents in the upper stream of the Yangtze River and its tributaries. In this study, the complete mitogenome sequence of J. sinensis has been first sequenced by DNA sequencing based on the PCR fragments. The mitogenome, consisting of 16,567 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNA genes and a noncoding control region (CR). The overall base composition of J. sinensis is 30.37% A, 28.82% C, 16.59% G, and 24.22% T, with a relatively a slight AT bias of 54.59%. CR of 903 bp length is located between tRNA(Pro) and tRNA(Phe). The complete mitochondrial genome may provide fundamental informative data not only for unravel the population structure and differentiation, but also for further conservation genetics studies on this balitorid species.
Assuntos
Cipriniformes/genética , Genoma Mitocondrial/genética , Animais , Composição de Bases/genética , Reação em Cadeia da Polimerase/métodos , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNA/métodosRESUMO
Homatula potanini is an endemic and one of ornamental fishes in the upper stream of the Yangtze River and its tributaries. However, wild populations of H. potanini declined sharply due to anthropological activity in the Jinsha River during the past decades. In present study, the complete mitochondrial DNA genome sequence of H. potanini was first determined by DNA sequencing based on the PCR fragments. The complete mitochondrial genome sequence is a circular molecule of 16,569 bp in size. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene nucleotide composition of H. potanini is 30.1% A, 26.9% C, 16.7% G, and 26.3% T, with a relatively high A + T content (56.4%). The results could provide useful data for studies on genetic structure and rational resource conservation in H. potanini.