The measurement of bile acid-dependent and independent fractions of rat bile.

Bile flow and bile acid secretory rate in rate in rats with interruption of the enterohepatic circulation had a biphasic decay with the second slower slope less marked for bile flow. A direct relationship between both parameters was observed in these animals. by knowing the choleretic efficiency of bile acids calculated from the regression line, the total bile acid output and the volume of bile collected, the fraction of bile produced by other way than bile water carried by bile acids was estimated. This fraction was found to be about 65 percent of the total bile formed.

[Effect of protein-free diet on sulfobromophthalein hepatic transport (author's transl)]. / Effecto de la dieta aproteica sobre el transporte hepatico de bromosulfaleina

Initial plasma disappearance rate and biliary excretion of intravenously injected sulfobromophthalein were studied in Wistar female rats fed a normal or a protein-free diet. Proteins and sulfobromophthalein distribution in the liver were studied by a gel filtration method. The results suggest that protein deficiency may produce an impaired sulfobromophthalein transference from plasma to liver cell due to a reduction of total hepatic proteins and of sulfobromophthalein-binding protein fraction responsible for liver uptake.