The experiences of dental hygienists providing restorative patient care.

OBJECTIVE: The intent of this inquiry was to explore the lived experiences of dental hygienists who provide restorative patient care. METHODS: Phenomenology was the qualitative approach used. Purposive sampling ensured participants had relevant experiences. An interview guide was emailed 1 week prior to the interview to help participants prepare responses. Informed consent included selecting a pseudonym, which was used throughout the interview and on the written transcript to protect the participants' identities. One-hour semi-structured interviews were conducted and recorded on Zoom. Data collection and analysis occurred simultaneously. Verbatim transcription accuracy was verified. Data were initially coded and then focused coding created themes. Demographic data were analysed using descriptive statistics. RESULTS: After eight interviews, data saturation was achieved. Seven females and one male participated. All participants experienced a restorative curriculum in their entry-level program and most felt well-prepared. The motivation for becoming a restorative dental hygienist included having positive restorative experiences during the entry-level program, wanting to keep restorative skills current after graduation, and having an opportunity to provide restorative care. Challenges experienced included the dentists' negative attitudes, hygienists' not valuing restorative abilities and not having a qualified dental assistant. Personal benefits were related to career fulfilment. Benefits to patients involved improving access, efficiency, and convenience. The benefit to the office was increased production. Differences between restorative and preventive care involved demands of care and patient care flow. CONCLUSION: The findings provide insight into the unique experiences of dental hygienists who provided restorative patient care and a foundation for future research.

Fighting for menstrual equity through period product pantries.

BACKGROUND: Menstruators facing period poverty often struggle with menstrual hygiene and waste management, which can result in harmful short- and long-term health outcomes such as urinary tract infections, yeast infections, and vulvar contact dermatitis. Research indicates that 42% of menstruators in the United States have difficulty affording period products. Traditional methods of distributing period products through social services may unintentionally undermine menstruators' agency, leading to disempowerment and inefficient resource allocation. Period product pantries are a novel approach aimed at addressing period poverty, inequity, and inadequate menstrual health education in the United States. OBJECTIVES: This paper aims to examine the development, organization, and implementation of two distinct period product pantry networks in Ohio and New York. It seeks to compare the advantages and challenges of grassroots versus nonprofit-led models and to provide practical insights for future pantry operators. DESIGN: The study examines two models of period product pantries: a grassroots effort led by three local residents in Ohio and an initiative spearheaded by a nonprofit organization in New York. The design includes a comparative analysis of both models' organization, funding methods, and operational structures. METHODS: The authors gathered data on the construction, operation, and usage of two pantry networks, focusing on factors such as accessibility, community engagement, and sustainability. The study employed a combination of qualitative methods, including interviews with organizers, and a review of organizational documents to analyze the effectiveness and scalability of each model. RESULTS: Both pantry networks increased accessibility to period products in low socioeconomic neighborhoods, which are disproportionately affected by period poverty. The grassroots model, while resource-limited, fostered strong community ties and local engagement. The nonprofit-led model benefited from dedicated staff and a more stable funding structure but faced bureaucratic challenges. Despite their differences, both models demonstrated the potential to empower menstruators by preserving their dignity and autonomy. CONCLUSIONS: Period product pantries represent an innovative and equitable approach to addressing period poverty and inequity. The analysis of the two models offers valuable insights for organizations and individuals interested in establishing similar initiatives. While each model has its unique benefits and challenges, both are effective in empowering menstruators and providing accessible menstrual hygiene products to those in need. REGISTRATION: Not applicable.

Fighting for menstrual equity through period product pantriesPeriod product pantries are a new way to help people who can't afford period products and don't have enough education about menstrual health in the U.S. Many people who experience period poverty, or trouble getting products like pads and tampons, also face barriers to staying clean and managing period waste. This can cause health issues like infections. About 42% of people who get periods in the U.S. say they've had trouble paying for these products. Period product pantries are different from older methods of getting free products, like through social services, because they let people get what they need without feeling embarrassed or losing their sense of control. This paper looks at two types of period pantries: one started by three local people in Ohio and another run by a nonprofit group in New York. Both help people in neighborhoods where it's hard to afford period products. The paper talks about how these pantries were set up, how they are funded, and what worked well or didn't. The goal is to show how these pantries can be a good, fair way to help people while giving advice to others who might want to start their own pantries.

Experiences of Dental Hygienists Providing Restorative Care.

Purpose The intent of this inquiry was to explore the lived experiences of dental hygienists who provide restorative patient care.Methods Phenomenology was the qualitative approach used. Purposive sampling ensured participants had relevant experiences. An Interview Guide was emailed one week prior to the interview to help participants prepare responses. Informed consent included selecting a pseudonym, which was used throughout the interview and on the written transcript to protect the participants' identities. One-hour semi-structured interviews were conducted and recorded on Zoom. Data collection and analysis occurred simultaneously. Verbatim transcription accuracy was verified. Data were initially coded and then focused coding created themes. Demographic data were analyzed using descriptive statistics.Results After eight interviews data saturation was achieved. Seven females and one male participated. All participants experienced a restorative curriculum in their entry-level program and most felt well-prepared. The motivation for becoming a restorative dental hygienist included having positive restorative experiences during the entry-level program, wanting to keep restorative skills current after graduation, and having an opportunity to provide restorative care. Challenges experienced included the dentists' negative attitudes, dental hygienists' not valuing restorative abilities and not having a qualified dental assistant. Personal benefits were related to career fulfillment. Benefits to patients involved improving access, efficiency, and convenience. The benefit to the office was increased production. Differences between restorative and preventive care involved demands of care and patient care flow.Conclusion The findings provide insight into the unique experiences of dental hygienists who provided restorative patient care and a foundation for future research.

Implementation and evaluation of a virtual dental home educational program.

AIMS: A Virtual Dental Home (VDH) is an alternative care model using teledentistry technology to provide care in community settings for special care populations. The Dental Hygiene Department at Idaho State University developed a VDH educational program to deliver preventive and therapeutic care at an assisted-living (AL) facility for memory care residents. The purpose of the educational program was to design and implement a VDH model for AL residents, and subsequently to evaluate the effectiveness of the educational program through students' knowledge, clinical confidence, and perspectives. METHODS AND RESULTS: Senior dental hygiene students (n = 32) completed didactic, laboratory, and clinical experiences on implementing an VDH. Using a pretest/posttest design, data were collected using a self-generated questionnaire; validity and reliability of the questionnaire were established prior to administration. Likert data were analyzed (n = 22, 69%) using the Wilcoxon Signed Rank test and Bonferroni correction. The change in scores of all three variables was statistically significant. The educational program was effective for increasing knowledge, clinical confidence, and perspectives of the dental hygiene students. CONCLUSION: Educational programs that include didactic, laboratory, and clinical experiences prepare graduates for using alternative care models, thereby, enhancing the potential to improve access to care for vulnerable populations in community settings.

Phage K gp102 Drives Temperature-Sensitive Antibacterial Activity on USA300 MRSA.

There is widespread interest in using obligately lytic bacteriophages ("phages") to treat human bacterial infections. Among Staphylococcus aureus infections, the USA300 lineage is a frequent cause of invasive disease. We observed that phage K, a model S. aureus myophage, exhibits temperature-sensitive growth on USA300 strains, with the wild-type phage providing poorer growth suppression in broth and forming smaller and fainter plaques at 37 °C vs. 30 °C. We isolated 65 mutants of phage K that had improved plaquing characteristics at 37 °C when compared to the parental phage. In all 65 mutants, this phenotype was attributable to loss-of-function (LoF) mutations in gp102, which encodes a protein of unknown function that has homologs only among the Herelleviridae (SPO1-like myophages infecting gram-positive bacteria). Additional experiments with representative mutants consistently showed that the temperature-sensitive plaque phenotype was specific to USA300 MRSA strains and that Gp102 disruption was correlated with improved suppression of bacterial growth in broth and improved antibacterial activity in a mouse model of upper respiratory tract infection. The same genotype and in vitro phenotypes could be replicated in close relatives of phage K. Gp102 disruption did not have a detectable effect on adsorption but did delay cell culture lysis relative to wild-type under permissive infection conditions, suggesting that gp102 conservation might be maintained by selective pressure for more rapid replication. Expression of gp102 on a plasmid was toxic to both an MSSA and a USA300 MRSA strain. Molecular modeling predicts a protein with two helix-turn-helix domains that displays some similarity to DNA-binding proteins such as transcription factors. While its function remains unclear, gp102 is a conserved gene that is important to the infection process of Kayvirus phages, and it appears that the manner in which USA300 strains defend against them at 37 °C can be overcome by gp102 LoF mutations.

Assessing the effects of tidal stream marine renewable energy on seabirds: A conceptual framework.

We are at a crossroads where many nation states, including the United Kingdom of Great Britain and Northern Ireland (UK), are committing to increased electricity production from "green energy", of which tidal stream marine renewable energy is one such resource. However, many questions remain regarding the effects of tidal energy devices on marine wildlife, including seabirds, of which the UK has internationally important numbers. Guidelines are lacking on how best to use both well-established and novel survey methods to assess seabird use of tidal flow areas, leading to a data-rich but information poor (DRIP) situation. This review provides a conceptual framework for assessing the effects of tidal stream energy devices on seabirds, summarises current knowledge and highlights knowledge gaps. Finally, recommendations are given for how best to pursue knowledge on this topic.

Rehabilitation of Cognitive Dysfunction Following Traumatic Brain Injury.

This article outlines key principles and considerations in the rehabilitation of cognitive challenges following mild, moderate, and severe traumatic brain injuries, with a focus on the needs of the service member and veteran population. The authors highlight specific evidence-based strategies and interventions and provide functional examples to support implementation. By emphasizing the array of tools and resources that have been designed to address cognitive challenges in the service member and veteran population, they focus on optimizing cognition to support successful community reintegration and the resumption of a full and meaningful life.

Involvement of alpha6/alpha3 neuronal nicotinic acetylcholine receptors in neuropsychiatric features of Dementia with Lewy bodies: [(125)I]-alpha-conotoxin MII binding in the thalamus and striatum.

Dementia with Lewy bodies (DLB) is a neurodegenerative disease associated with a range of neuropsychiatric symptoms and reduced expression of neuronal nicotinic acetylcholine receptors (nAChRs) in neocortex, hippocampus, thalamus and basal ganglia. To determine whether there are selective associations between alterations in alpha6/alpha3 neuronal nicotinic acetylcholine receptors (nAChRs) and the two key neuropsychiatric features of DLB, impaired consciousness (IC) and visual hallucinations (VH), quantitative [(125)I]-alpha-conotoxin MII ([(125)I]-alpha-Ctx MII) autoradiography was undertaken on 28 people with DLB and 15 control cases from the Newcastle Brain Bank. There was a highly significant overall trend for reduced thalamic [(125)I]-alpha-Ctx MII binding in DLB (p < 0.001), with significant deficits in the centromedian, ventral lateral and ventroposterior medial thalamic nuclei (p < 0.05), together with caudate and putamen (p < 0.001). [(125)I]-alpha-Ctx MII binding was significantly lower in DLB cases with IC than without IC in the putamen (p < 0.05), however there was no significant association between [(125)I]-alpha-Ctx MII binding and VH. Reductions in [(125)I]-alpha-Ctx MII binding in caudate and putamen were paralleled by similar reductions in [(125)I]PE2I binding. [(125)I]PE2I binding was also significantly lower in DLB cases with IC than without IC in the caudate (p < 0.05) and putamen (p < 0.001). These results demonstrate that deficits in alpha6/alpha3 nAChRs occur in specific brain regions in DLB, may in part be related to the loss of dopaminergic neurons and may contribute to the development of impaired consciousness in the disorder.

Plastic biochannel hybridization devices: a new concept for microfluidic DNA arrays.

Conventional DNA hybridization assay kinetics depends solely on the diffusion of target to surface-bound probes, causing long hybridization times. In this study, we examined the possibilities of accelerating the hybridization process by using microfluidic channels ("biochannels") made of polycarbonate, optionally with an integrated pump. We produced two different devices to study these effects: first, hybridization kinetics was investigated by using an eSensor electrochemical DNA detection platform allowing kinetic measurements in homogenous solution. We fabricated an integrated cartridge for the chip comprising the channel network and a micropump for the oscillation of the hybridization mixture to further overcome diffusion limitations. As a model assay, we used an assay for the detection of single-nucleotide polymorphisms in the HFE-H gene. Second, based on the biochannel approach, we constructed a plastic microfluidic chip with a network of channels for optical detection of fluorescent-labeled targets. An assay for the simultaneous detection of four pathogenic bacteria surrogate strains from multiple samples was developed for this device. We observed high initial hybridization velocities and a fast attainment of equilibrium for the biochannel with integrated pump. Experimental results were compared with predictions generated by computer simulations.