RESUMO
OBJECTIVES: To characterize nitric oxide synthase (NOS), which catalyzes nitric oxide (NO) production, in the human prostate using biochemical and immunohistochemical techniques. METHODS: NOS catalytic assay and NOS immunohistochemistry were performed on histologically verified nonmalignant prostate tissue obtained from the peripheral and transition zones of seven radical prostatectomy specimens. RESULTS: Biochemical analysis revealed NOS activity in the human prostate, with a greater amount in the peripheral zone than in the transition zone (P < 0.01). In both prostate zones, NOS was immunohistochemically localized to nerve fibers and ganglia coursing throughout the smooth musculature of the stroma and to subepithelial nerve plexuses. NOS immunoreactivity was also localized to glandular epithelium. CONCLUSIONS: The presence, activity, and distribution of NOS were described in two regions of the human prostate. The present evidence implicates NO in the automatic innervation and physiology of the human prostate. It is proposed that NO may modulate smooth muscle tone and secretory functions in the human prostate, although functional studies are needed to support these hypotheses.
Assuntos
Aminoácido Oxirredutases/análise , NADPH Desidrogenase/análise , Próstata/química , Humanos , Masculino , Óxido Nítrico Sintase , Próstata/enzimologiaRESUMO
Evidence is provided for the existence of platelet-activating factor (PAF)-like activity in the lipid extracts of human spermatozoa. The PAF content of human spermatozoa based on [3H]-serotonin release from washed rabbit platelets was noted to be 1.45 pmol/10(8) sperm cells in highly purified motile spermatozoa. No PAF activity was associated with the seminal fluid. Platelet-activating factor content of spermatozoa may be related to its fertility potential.
Assuntos
Fosfolipídeos/isolamento & purificação , Fator de Ativação de Plaquetas/análise , Espermatozoides/química , Animais , Plaquetas/efeitos dos fármacos , Plaquetas/metabolismo , Cromatografia Líquida de Alta Pressão , Humanos , Lipídeos/isolamento & purificação , Masculino , Fator de Ativação de Plaquetas/farmacologia , Coelhos , Serotonina/sangue , Motilidade dos Espermatozoides , Espermatozoides/fisiologiaRESUMO
Our laboratory has recently detected the presence of platelet-activating factor (PAF)-like activity in human spermatozoa. To gain further insight into the role of PAF on the male reproductive system, this study, using videomicroscopy, evaluated the effects of synthetic PAF on the motility of human spermatozoa. Treatment of 20 human semen samples with 3.69 x 10(-7) to 3.69 x 10(-13) M PAF resulted in statistically significant increases in motility. Treatment of spermatozoa with lyso-PAF (the biologically inactive form of PAF) showed no change in motility. Treatment of spermatozoa with PAF in severely asthenozoospermic males may be of therapeutic value.
Assuntos
Fator de Ativação de Plaquetas/farmacologia , Motilidade dos Espermatozoides/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , Masculino , Microscopia , Concentração Osmolar , Espermatozoides/efeitos dos fármacos , TelevisãoRESUMO
OBJECTIVE: To determine if nitric oxide synthase activity within the male reproductive tract is regulated by androgen. DESIGN: Nitric oxide synthase activity was measured in the reproductive organs of three groups of mature rats: unoperated controls, 1-week castrates, and 1-week castrates given T capsules at the time of surgery. The presence of nitric oxide synthase activity was confirmed by using the nitric oxide synthase-specific inhibitor N-nitro-L-arginine methyl ester (L-NAME). RESULTS: After castration, nitric oxide synthase activity was significantly reduced by 88%, 73%, and 54% in the caput, corpus, and cauda epididymidis, respectively. In the penis, nitric oxide synthase activity decreased 45% and nitric oxide synthase protein decreased 57% after castration. In the seminal vesicle and lateral prostate, nitric oxide synthase activity increased significantly after castration from nondetectable levels in controls. Nitric oxide synthase activity in the coagulating gland and ventral and dorsal prostate did not change after castration. The changes in nitric oxide synthase activity in all organs after castration were prevented by T replacement. Additionally, the activity measured in every organ in all three treatment groups was > 90% inhibited by L-NAME. CONCLUSION: These data demonstrate that androgen differentially affects nitric oxide synthase activity in the male reproductive tract. To the best of our knowledge this is the first time that nitric oxide synthase activity has been shown to be influenced by androgen in any tissue.
Assuntos
Aminoácido Oxirredutases/metabolismo , Androgênios/fisiologia , Genitália Masculina/enzimologia , Aminoácido Oxirredutases/antagonistas & inibidores , Animais , Arginina/análogos & derivados , Arginina/farmacologia , Cálcio/farmacologia , Epididimo/enzimologia , Masculino , NG-Nitroarginina Metil Éster , Óxido Nítrico Sintase , Orquiectomia , Pênis/enzimologia , Próstata/enzimologia , Ratos , Ratos Sprague-Dawley , Glândulas Seminais/enzimologia , Testosterona/farmacologia , Ducto Deferente/enzimologiaRESUMO
Sympathetic denervation of the rat cauda epididymidis by surgical removal of the inferior mesenteric ganglion (IMG) results in an excessive accumulation of sperm in the cauda epididymidis as well as significant changes in cauda sperm motility and cauda epididymal gross histology. The objective of the present study was to determine if the cauda-specific changes in sperm storage, sperm motility, and epididymal histology following the loss of sympathetic innervation were accompanied by changes in the protein composition of epididymal fluid. One and 4 weeks after surgical IMG removal or sham operations, luminal fluid obtained from the caput and cauda epididymidis and cauda epididymal sperm-associated proteins were subjected to two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and silver-stained proteins were quantitated. One week after IMG removal, two cauda epididymal fluid (CEF) proteins (2 and 13) had increased 43% and 49%, respectively, whereas four CEF proteins (5, 8, 9, and 19) had decreased between 30% and 73% compared to controls. Four weeks after IMG removal, changes in CEF proteins observed 1 week following surgery were no longer present, but the staining intensities of three additional CEF proteins (11, 12, and 18) were reduced an average of 70% compared to control CEF proteins. By obstructing the cauda epididymidis, we confirmed that the changes in CEF protein composition observed following IMG removal were not the result of sperm accumulation but were due directly to the loss of innervation; the staining intensity of CEF protein 2 increased as a result of excessive sperm accumulation in the cauda epididymidis both in the presence and absence of innervation from the IMG. No significant changes in caput epididymal fluid proteins or cauda epididymal sperm-associated proteins were detected following IMG removal. These data show that the protein composition of rat CEF is significantly affected by the loss of sympathetic innervation and suggest that neuronal input may play an important role in the maintenance of epididymal function.
Assuntos
alfa-Globulinas/química , Denervação Autônoma , Epididimo/metabolismo , Plexo Hipogástrico/cirurgia , Metaloproteínas/química , Proteínas/química , Hormônios Testiculares/química , Animais , Eletroforese em Gel de Poliacrilamida , Proteínas Secretadas pelo Epidídimo , Epididimo/inervação , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
The rat cauda epididymidis receives sympathetic innervation from the inferior mesenteric ganglion (IMG). We have previously demonstrated that surgical removal of the IMG and proximal hypogastric nerves (IMG denervation) results in significant and cauda-specific changes in epididymal sperm transport, sperm motility, luminal fluid protein composition, and tissue histology. In the present study we used natural mating trials and intrauterine insemination (IUI) techniques to determine whether or not IMG denervation affects male fertility and reproductive capacity. For the initial studies, adult male Sprague Dawley rats were mated with estrous females 1 and 4 weeks following IMG denervation. Nine days after mating, uterine implantation sites and corpora lutea (CL) were counted. In females mated with sham-operated control males, 85.8% of ovulated oocytes were fertilized and subsequently implanted. In contrast, females mated with IMG-denervated males 1 or 4 weeks following surgery had 0% and 3.5%, respectively, of ovulated oocytes fertilized and implanted. For rats maintained 21 days after mating, an average of 13 +/- 1 pups were delivered by each of nine females mated with sham-operated control male rats; whereas, only seven morphologically normal pups were delivered by one of 14 females mated with IMG-denervated male rats. Additional experiments demonstrated that the decrement in offspring was, in part, due to a significant decrease in the number of spermatozoa in the female uterus following mating with IMG-denervated males. To determine whether IMG denervation exerted an additional effect directly on the fertilizing ability of spermatozoa, IUI experiments were performed. Six million cauda epididymal spermatozoa from 1- or 4-week IMG-denervated males were inseminated into the uterine horns of luteinzing hormone-releasing hormone (LHRH)-synchronized females and 9 days later implantation sites and CL were counted. Implantations were observed for 78%, 28%, and 25% of ovulated oocytes following IUI with spermatozoa from sham-operated controls and from 1- and 4-week IMG-denervated rats, respectively. To determine whether the reduction in implantation sites following IUI with spermatozoa from IMG-denervated rats resulted from impaired oocyte fertilization, studies were performed in which oocytes were retrieved and stained 24 hours after IUI. Comparable fertilization rates of 76.5% and 89.0% were observed using cauda epididymal spermatozoa from IMG-denervated and sham-operated control males, respectively, indicating that oocyte fertilization was not affected by the loss of innervation. These studies establish the importance of innervation from the IMG for ejaculatory competence and sperm reproductive capacity in the male rat. These data further suggest that sympathetic innervation in the epididymis critically influences paternal factors associated with embryonic development.
Assuntos
Denervação , Desenvolvimento Embrionário e Fetal , Epididimo/fisiologia , Fertilização , Espermatozoides/fisiologia , Sistema Nervoso Simpático/fisiologia , Animais , Copulação , Implantação do Embrião , Epididimo/inervação , Tubas Uterinas/fisiologia , Feminino , Inseminação Artificial , Masculino , Ovário/fisiologia , Gravidez , Ratos , Ratos Sprague-Dawley , Motilidade dos EspermatozoidesRESUMO
Sympathetic nerves emanating from the inferior mesenteric ganglion (IMG) innervate the mammalian epididymis and vas deferens. We have reported previously that surgical removal of the rat IMG results in excessive accumulation of spermatozoa within the cauda epididymis. The objective of the present study was to determine whether sperm accumulation following IMG removal was due to a denervation-induced change in the rate of sperm transport within the cauda epididymis. In these studies epididymal sperm numbers were counted and used as a measure of sperm transport within the epididymis. In order to examine sperm transport within the cauda epididymis specifically, efferent duct ligation (EDL) was used to prevent further entry of testicular spermatozoa into the epididymis. Rats were divided into four treatment groups: sham-operated control, EDL alone for 7 days (EDL + 7), EDL alone for 14 days (EDL + 14), or EDL for 7 days at which time the IMG was removed and the rats were maintained for an additional 7 days (EDL + IMG). Epididymides were homogenized and the number of spermatozoa in the caput and cauda epididymis was counted. In EDL + 7 rats, the caput epididymis was devoid of spermatozoa. The rate of transit of spermatozoa through the cauda epididymis of EDL + 7 rats was approximately 3.43 million/day. The total number of cauda epididymal spermatozoa in EDL + 7 rats was reduced by 20% compared to sham-operated control rats. In EDL + 14 rats, spermatozoa transited the cauda epididymis at a rate of approximately 9.57 million/day, and the total number of spermatozoa in the cauda epididymis was reduced by 73% compared to sham-operated controls. If the IMG was removed 7 days following EDL, spermatozoa transited the cauda epididymis at a rate comparable to that observed in EDL + 7 rats (3.39 million/day). Following IMG denervation of EDL + 7 rats, cauda epididymal sperm numbers were maintained at levels observed in the EDL + 7 rats. These data demonstrate that the transport of spermatozoa through the cauda epididymis is influenced significantly by neuronal input from the IMG.
Assuntos
Epididimo/citologia , Gânglios Simpáticos/fisiologia , Neurônios/fisiologia , Motilidade dos Espermatozoides/fisiologia , Animais , Denervação , Epididimo/inervação , Gânglios Simpáticos/citologia , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
This study was conducted to investigate the requirement for sperm processing in microsurgical subzonal placement of sperm in rabbit oocytes. Fertilization rates with standard in vitro fertilization and microsurgical subzonal sperm placement were found to be similar (56 and 55%) when sperm treated with high-ionic strength Brackett's defined medium to initiate capacitation were used. Statistically significant reductions in fertilization rates for both standard in vitro fertilization and subzonal placement were noted when twice-washed spermatozoa were used. Initiation of capacitation of spermatozoa results in higher fertilization results even when the zona pellucida is bypassed during fertilization.
Assuntos
Fertilização in vitro/métodos , Capacitação Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Zona Pelúcida , Animais , Blastocisto , Sobrevivência Celular , Meios de Cultura , Transferência Embrionária , Feminino , Masculino , Microinjeções , Oócitos/citologia , Concentração Osmolar , CoelhosRESUMO
Capacitation of spermatozoa is essential for fertilization. Rabbit spermatozoa are particularly difficult to capacitate in vitro and require treatment with high-ionic-strength Brackett's defined medium. Spermatozoa treated with platelet activating factor had significantly higher fertilization rates when compared with nontreated (fresh, twice washed) spermatozoa (63% vs 34%). Fertilization rates of spermatozoa treated with platelet activating factor, although higher than those of high-ionic-strength capacitated spermatozoa, were not significantly different (63% vs 57%). Spermatozoa treated with lyso-platelet activating factor, the biologically inactive form of platelet activating factor, were noted to have fertilization rates similar to those of the untreated (noncapacitated) group. These data show that synthetic platelet activating factor treatment of uncapacitated spermatozoa induces fertilization of rabbit oocytes in vitro in a manner similar to that for spermatozoa capacitated by high-ionic-strength media and significantly higher than that for untreated spermatozoa or after treatment with the biologically inactive form of platelet activating factor (lyso-platelet activating factor).
Assuntos
Fertilização in vitro , Oócitos/fisiologia , Fator de Ativação de Plaquetas/farmacologia , Animais , Feminino , Fertilização , Masculino , Fator de Ativação de Plaquetas/análogos & derivados , Gravidez , Coelhos , Capacitação Espermática , Espermatozoides/fisiologiaRESUMO
Platelet activating factor is rapidly gaining acceptance as a potent mediator in many reproductive processes. This study presents data that indicate a direct role of platelet activating factor in fertilization. Platelet activating factor was shown to significantly increase (p less than 0.001) the fertilization rate of mouse oocytes in vitro. Furthermore, CV3988, an inhibitor of platelet activating factor, was noted to significantly decrease in vitro fertilization rates at 10(-5) and 10(-4) mol/L concentrations.
Assuntos
Fertilização/efeitos dos fármacos , Oócitos/efeitos dos fármacos , Fator de Ativação de Plaquetas/farmacologia , Animais , Feminino , Fertilização/fisiologia , Masculino , Camundongos , Oócitos/patologia , Éteres Fosfolipídicos/farmacologia , Fator de Ativação de Plaquetas/antagonistas & inibidores , Fator de Ativação de Plaquetas/fisiologiaRESUMO
Nitric oxide synthase (NOS), which catalyzes the production of nitric oxide (NO), was characterized within the reproductive tract of adult male Sprague-Dawley rats by means of biochemical and immunohistochemical techniques. Tissues examined included the testis, epididymis (caput, corpus, and cauda regions), vas deferens, ejaculatory duct, seminal vesicle, and coagulating gland. NOS activity was measured by use of an assay based on the stoichiometric conversion of [3H]-L-arginine to [3H]-L-citrulline and NO, catalyzed by NOS. Low levels of NOS activity were detected in the testis and seminal vesicle (< 0.5 fmol [3H]-L-citrulline formed/min/mg protein in each tissue). The highest levels of NOS activity were present in the cauda segment of the epididymis and in the vas deferens, each having a sevenfold greater amount of NOS activity than the testis (p < 0.05). Intermediate levels of NOS activity were detected in the coagulating gland (0.863 +/- 0.248 fmol [3H]-L-citrulline formed/min/mg protein), caput epididymidis (0.457 +/- 0.180 fmol [3H]-L-citrulline formed/min/mg protein), and corpus epididymidis (0.631 +/- 0.215 fmol [3H]-L-citrulline formed/min/mg protein). NADPH diaphorase histochemistry and NOS immunohistochemistry localized NOS to neuronal fibers coursing throughout the smooth musculature and subepithelial regions of the epididymis, vas deferens, and ejaculatory duct. Endothelial cells and nerve plexuses within the adventitia of blood vessels supplying reproductive tissues were also positive for NOS. Additional localizations of NOS were within epithelial cells of the epididymis and coagulating gland.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Aminoácido Oxirredutases/metabolismo , Genitália Masculina/enzimologia , Animais , Ductos Ejaculatórios/enzimologia , Epididimo/enzimologia , Histocitoquímica , Imuno-Histoquímica , Masculino , NADPH Desidrogenase , Óxido Nítrico Sintase , Ratos , Ratos Sprague-Dawley , Glândulas Seminais/enzimologia , Testículo/enzimologia , Distribuição Tecidual , Ducto Deferente/enzimologiaRESUMO
The regulation and possible function of the preproenkephalin gene in testis were studied in vivo in transgenic mice containing: (1) bases -193 to +210 of the human proenkephalin gene and an additional one kilobase of 3' proenkephalin flanking sequence driving expression of bacterial chloramphenicol acetyltransferase (CAT), and (2) the same promoter and flanking sequences driving expression of a rat proenkephalin cDNA. Five lines of mice, designated HEC1-5, expressed the first construct and 10, HER1-10, the second. Each HEC male and many HER males showed dramatic expression of the transgene in the testis, although much lower expression was observed in the brain and other enkephalin-producing tissues. High levels of expression in testis can thus be achieved with a very short promoter region and do not require intron A sequences previously considered necessary. Altered enkephalin expression may affect testicular function. One founder, HER8, displayed grossly abnormal testicular morphology and was completely infertile. A second founder, HER6, had low sperm motility. Two offspring from other lines also displayed subnormal fertility. These studies support a role for specific promoter sequences in testis expression and may further support a significant role for proenkephalin in testicular function.
Assuntos
Encefalinas/genética , Precursores de Proteínas/genética , Animais , Sequência de Bases , Sondas de DNA/genética , DNA Complementar/genética , Feminino , Efeito Fundador , Regulação da Expressão Gênica , Humanos , Infertilidade Masculina/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Ratos , Espermatogênese/genética , Espermatogênese/fisiologia , Testículo/metabolismo , Testículo/ultraestruturaRESUMO
Guanethidine, a chemical that selectively blocks sympathetic noradrenergic neurons, was used to investigate the role of sympathetic innervation in the fertility of rat epididymal sperm, using both natural mating and in utero insemination protocols. This animal model correlates, at least in part, with spinal cord injury (SCI) in men. Adult male rats were treated daily by i.p. injections, for 21 or 42 days, with 0 or 6.25 mg/kg guanethidine. To compare the effects of guanethidine-induced sympathectomy with those following surgically induced sympathectomy, the inferior mesenteric ganglion and the proximal hypogastric nerves were removed in another group of rats. Both chemically and surgically induced sympathectomy increased the weight of the epididymis and seminal vesicles/coagulating glands as well as the number and the transit time of cauda epididymal sperm. Neither serum testosterone levels nor LH was affected by treatment with guanethidine. Using natural mating, no litters were produced by guanethidine-treated rats. Chemically denervated rats failed to produce copulatory plugs or ejaculate into the uterus. However, distal cauda epididymal sperm from chemically or surgically denervated rats displayed normal fertilization ability (80%) using in utero inseminations. In addition, the sperm of denervated rats did not show abnormal sperm chromatin structure using an assay that detects DNA damage. We conclude that sympathectomy delays the transit of sperm through the cauda epididymidis and produces ejaculatory dysfunction but does not compromise sperm quality in the distal cauda epididymidis. Moreover, these data provide compelling evidence that there is no association between the prolonged transit time of sperm within the epididymis, i.e., pre-ejaculatory sperm aging, and the fertility of those sperm, which has important implications for artificial insemination using sperm from men with SCI.