1.
Antimicrob Agents Chemother
; 49(1): 444-6, 2005 Jan.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15616332
RESUMO
In this study the nucleotide sequence of the pncA gene from 59 Mycobacterium tuberculosis clinical isolates was analyzed. Mutations in the pncA gene were identified in 29 of 40 pyrazinamide-resistant isolates, and no pyrazinamidase activity was detected in 39 of them. Twelve mutations found in this work have not been described previously.
Assuntos
Amidoidrolases/genética , Antituberculosos/farmacologia , Farmacorresistência Bacteriana/genética , Mutação , Mycobacterium tuberculosis/efeitos dos fármacos , Pirazinamida/farmacologia , Testes de Sensibilidade Microbiana , Mycobacterium tuberculosis/enzimologia , Mycobacterium tuberculosis/genética , Tuberculose/microbiologia
2.
J Clin Microbiol
; 40(6): 2297-9, 2002 Jun.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12037115
RESUMO
Sixty-nine blood samples from 47 patients infected with human immunodeficiency virus were analyzed by using PCR to detect Mycobacterium avium. The sensitivity can be up to 95.7%, depending on the detection method used and the number of blood samples analyzed from each patient. The procedure can be helpful in the diagnosis of mycobacterial disease.