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1.
J Cell Biol ; 87(1): 180-96, 1980 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-7191426

RESUMO

In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. ER cisternae are also situated very close to "rosettes,"plasmalemmal specializations found in mature adipocytes in vivo. As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. Other features described for the first time in 3T3-L1 preadipocytes include: (a) the presence of an extensive acid phosphatase (AcPase) positive GERL from which coated vesicles apparently arise (these coated vesicles display AcPase activity and are much smaller and far more numerous than the coated vesicles that seem to arise from the plasmalemmal coated pits); (b) the abundance of AcPase-positive autophagic vacuoles; and (c) a high level of alpha-naphthyl-acetate-esterase activity which, by light microscopy cytochemistry, appears to be localized in the cytosol.


Assuntos
Tecido Adiposo/ultraestrutura , Animais , Diferenciação Celular , Membrana Celular/ultraestrutura , Núcleo Celular/ultraestrutura , Células Cultivadas , Grânulos Citoplasmáticos/ultraestrutura , Citoesqueleto/ultraestrutura , Retículo Endoplasmático/ultraestrutura , Complexo de Golgi/ultraestrutura , Lisossomos/ultraestrutura , Camundongos , Microcorpos/ultraestrutura , Fagocitose
2.
J Cell Biol ; 98(4): 1342-7, 1984 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-6325469

RESUMO

While a multiplicity of cellular and biochemical effects are mediated by interferons on cultured cells, the mechanisms involved in the direct growth-inhibitory activity of interferons remain problematic. We have previously found that variants in cAMP metabolism in a macrophage cell line, J774.2, were at least 50-fold less sensitive to the growth inhibitory activity of interferons (IFN) than the parental clone. To test the hypothesis that cAMP mediates the growth inhibition produced by IFN in these cells, interferon-resistant variants were selected and characterized with respect to cAMP synthesis and function. Approximately one-third of the IFN-resistant clones were found to be resistant to growth inhibition produced by cholera toxin, but not 8Br-cAMP. IFN was fully able to protect all of the interferon-resistant/choleratoxin-resistant (IFNr/CTr) clones against infection by vesicular stomatitis virus and markedly stimulated 2', 5'-oligodenylate synthetase activity. These IFNr/CTr variants were shown to have a defect in adenylate cyclase. The remaining IFN-resistant clones were fully susceptible to the growth-inhibitory effects of cholera toxin because their basal and stimulated adenylate cyclase activity is similar to that of the parental clone. IFN failed to protect these IFNr/choleratoxin sensitive clones against infection by vesicular stomatitis virus and failed to stimulate 2', 5-oligodenylate synthetase, suggesting that they have defective or deficient IFN receptors. In addition, IFN failed to increase intracellular cAMP levels in both IFNr/CTr and IFNr/choleratoxin sensitive clones. These results provide firm genetic and biochemical evidence that the growth inhibitory effects of IFN on this cell line are mediated by cAMP.


Assuntos
Interferon Tipo I/toxicidade , Macrófagos/fisiologia , Mutação , 2',5'-Oligoadenilato Sintetase/metabolismo , Adenilil Ciclases/metabolismo , Animais , Divisão Celular/efeitos dos fármacos , Membrana Celular/ultraestrutura , Células Clonais , AMP Cíclico/metabolismo , Resistência a Medicamentos , Variação Genética , Linfoma Difuso de Grandes Células B , Macrófagos/efeitos dos fármacos , Camundongos , Vírus da Estomatite Vesicular Indiana/efeitos dos fármacos
3.
J Cell Biol ; 91(3 Pt 1): 798-802, 1981 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-6120173

RESUMO

Trifluoperazine, a drug that binds to Ca2+-calmodulin and inhibits its interaction with other proteins, was found to inhibit growth and phagocytosis in a macrophagelike cell line, J774.16. Both effects were reversible and occurred at the same concentrations of drug (25--50 microM) that inhibited the activation of cyclic nucleotide phosphodiesterase by calmodulin in vitro. Fc-mediated phagocytosis was also depressed by W-7, a sulfonamide derivative that inhibits the activity of Ca2+-calmodulin. In contrast, taxol, a drug that stabilizes cellular microtubules, had no effect on Fc-mediated phagocytosis although it inhibited cell growth at nanomolar concentrations. The inhibitory effects of trifluoperazine and W-7 on phagocytosis suggest that calmodulin may be involved in this complex cellular function.


Assuntos
Proteínas de Ligação ao Cálcio/antagonistas & inibidores , Calmodulina/antagonistas & inibidores , Macrófagos/fisiologia , Fagocitose/efeitos dos fármacos , Trifluoperazina/farmacologia , Alcaloides/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Camundongos , Muramidase/metabolismo , Paclitaxel , Sulfonamidas/farmacologia
4.
Science ; 237(4821): 1452-8, 1987 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-2442814

RESUMO

Three recent advances pertinent to the mechanism of insulin action include (i) the discovery that the insulin receptor is an insulin-dependent protein tyrosine kinase, functionally related to certain growth factor receptors and oncogene-encoded proteins, (ii) the molecular cloning of the insulin proreceptor complementary DNA, and (iii) evidence that the protein tyrosine kinase activity of the receptor is essential for insulin action. Efforts are now focusing on the physiological substrates for the receptor kinase. Experience to date suggests that they will be rare proteins whose phosphorylation in intact cells may be transient. The advantages of attempting to dissect the initial biochemical pathway of insulin action include the wealth of information about the metabolic consequences of insulin action and the potential for genetic analysis in Drosophila and in man.


Assuntos
Insulina/metabolismo , Receptor de Insulina/fisiologia , DNA/análise , Drosophila , Receptores ErbB/fisiologia , Humanos , Peso Molecular , Oncogenes , Fosforilação , Fosfosserina/metabolismo , Fosfotreonina/metabolismo , Fosfotirosina , Proteínas Tirosina Quinases/metabolismo , Receptor de Insulina/genética , Receptores de Superfície Celular/metabolismo , Especificidade por Substrato , Tirosina/análogos & derivados , Tirosina/metabolismo
5.
Science ; 235(4795): 1495-8, 1987 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-3029870

RESUMO

Elevation of glucose transport is an alteration common to most virally induced tumors. Rat fibroblasts transformed with wild-type or a temperature-sensitive Fujinami sarcoma virus (FSV) were studied in order to determine the mechanisms underlying the increased transport. Five- to tenfold increases in total cellular glucose transporter protein in response to transformation were accompanied by similar increases in transporter messenger RNA levels. This, in turn, was preceded by an absolute increase in the rate of glucose transporter gene transcription within 30 minutes after shift of the temperature-sensitive FSV-transformed cells to the permissive temperature. The transporter messenger RNA levels in transformed fibroblasts were higher than those found in proliferating cells maintained at the nonpermissive temperature. The activation of transporter gene transcription by transformation represents one of the earliest known effects of oncogenesis on the expression of a gene encoding a protein of well-defined function.


Assuntos
Transformação Celular Viral , Proteínas de Transporte de Monossacarídeos/genética , Animais , Vírus do Sarcoma Aviário , Divisão Celular , Linhagem Celular , Fibroblastos , Regulação da Expressão Gênica , Cinética , RNA Mensageiro/genética , Ratos , Transcrição Gênica
6.
Mol Cell Biol ; 9(7): 2806-17, 1989 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2476658

RESUMO

Insulin and insulinlike growth factor 1 (IGF-1) receptors are present in brain, yet their function remains obscure. Expression of these tyrosine kinase-bearing growth factor receptors during rat brain development was examined by using three antipeptide antibodies directed against epitopes in the beta subunits (AbP2, AbP4, and AbP5). All three antibodies recognized both insulin and IGF-1 receptors. Membranes were prepared from fetal brains (14 to 21 days of gestation), neonatal brain (postnatal day 1), and adult brain. Immunoblot analyses using AbP4 and AbP5 revealed a 92-kilodalton (kDa) protein that corresponded to the beta subunit of the insulin and IGF-1 receptors. Densitometric scanning of immunoblots indicated that receptor proteins were 4- to 10-fold more abundant in fetal brain membranes than in membranes from adult brain. Expression was highest during 16 to 18 days of gestation and declined thereafter to the relatively low level found in adult brain. Immunoblot analyses with AbP2 as well as ligand-activated receptor autophosphorylation revealed an additional protein of 97 kDa. This protein was phosphorylated in response to IGF-1 and was not directly recognized by AbP4 or AbP5. The covalent association of the 97-kDa protein with the 92-kDa beta subunit was indicated by the ability of AbP4 and AbP5 to immunoprecipitate both proteins under nonreducing conditions but only the 92-kDa protein after reduction. In contrast, AbP2 immunoprecipitated both proteins regardless of their association. This immunospecificity remained unchanged after deglycosylation of the isolated proteins. Two-dimensional tryptic phosphopeptide analysis showed that the 92- and 97-kDa subunits of the IGF-1 receptor are related but distinct proteins. Taken together, the data suggest that the 92- and 97-kDa subunits differ in primary amino acid sequence. Thus, two distinct beta subunits may be present in a single IGF-1 receptor in brain. These subunits have in common an epitope recognized by an antibody to the tyrosine kinase domain (AbP2) but differ in regions thought to be important in receptor kinase regulation and signal transduction.


Assuntos
Encéfalo/metabolismo , Receptor de Insulina/metabolismo , Receptores de Superfície Celular/metabolismo , Animais , Western Blotting , Encéfalo/embriologia , Encéfalo/crescimento & desenvolvimento , Eletroforese em Gel de Poliacrilamida , Epitopos/imunologia , Fator de Crescimento Insulin-Like I/metabolismo , Mapeamento de Peptídeos , Fosforilação , Testes de Precipitina , Proteínas Tirosina Quinases/metabolismo , Ratos , Receptor de Insulina/imunologia , Receptores de Superfície Celular/imunologia , Receptores de Somatomedina
7.
Mol Cell Biol ; 9(10): 4187-95, 1989 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-2479821

RESUMO

We report the functional expression of two different mammalian facilitative glucose transporters in Xenopus oocytes. The RNAs encoding the rat brain and liver glucose transporters were transcribed in vitro and microinjected into Xenopus oocytes. Microinjected cells showed a marked increase in 2-deoxy-D-glucose uptake as compared with controls injected with water. 2-Deoxy-D-glucose uptake increased during the 5 days after microinjection of the RNAs, and the microinjected RNAs were stable for at least 3 days. The expression of functional glucose transporters was dependent on the amount of RNA injected. The oocyte-expressed transporters could be immunoprecipitated with anti-brain and anti-liver glucose transporter-specific antibodies. Uninjected oocytes expressed an endogenous transporter that appeared to be stereospecific and inhibitable by cytochalasin B. This transporter was kinetically and immunologically distinguishable from both rat brain and liver glucose transporters. The uniqueness of this transporter was confirmed by Northern (RNA) blot analysis. The endogenous oocyte transporter was responsive to insulin and to insulinlike growth factor I. Most interestingly, both the rat brain and liver glucose transporters, which were not insulin sensitive in the tissues from which they were cloned, responded to insulin in the oocyte similarly to the endogenous oocyte transporter. These data suggest that the insulin responsiveness of a given glucose transporter depends on the type of cell in which the protein is expressed. The expression of hexose transporters in the microinjected oocytes may help to identify tissue-specific molecules involved in hormonal alterations in hexose transport activity.


Assuntos
Proteínas de Transporte de Monossacarídeos/genética , Oócitos/metabolismo , Animais , Transporte Biológico/genética , Citocalasina B/metabolismo , Desoxiglucose/metabolismo , Insulina/fisiologia , Fator de Crescimento Insulin-Like I/fisiologia , Microinjeções , Proteínas de Transporte de Monossacarídeos/biossíntese , Plasmídeos , RNA/metabolismo , Ratos , Proteínas Recombinantes/biossíntese , Xenopus laevis
8.
Mol Cell Biol ; 8(4): 1638-47, 1988 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2454394

RESUMO

The Drosophila melanogaster insulin receptor (Drosophila insulin receptor homolog [dIRH]) is similar to its mammalian counterpart in deduced amino acid sequence, subunit structure, and ligand-stimulated protein tyrosine kinase activity. The function of this receptor in D. melanogaster is not yet known. However, a role in development is suggested by the observations that levels of insulin-stimulated kinase activity and expression of dIRH mRNA are maximal during Drosophila midembryogenesis. In this study, a 2.9-kilobase (kb) cDNA clone corresponding to both the dIRH tyrosine kinase domain and some of the 3' untranslated sequence was used to determine the tissue distribution of dIRH mRNA during development. Two principal mRNAs of 11 and 8.6 kb hybridized with the dIRH cDNA in Northern (RNA) blot analysis. The abundance of the 8.6-kb mRNA increased transiently in early embryos, whereas the 11-kb species was most abundant during midembryogenesis. A similar pattern of expression was previously determined by Northern analysis, using a dIRH genomic clone (L. Petruzzelli, R. Herrera, R. Arenas-Garcia, R. Fernandez, M. J. Birnbaum, and O. M. Rosen, Proc. Natl. Acad. Sci. USA 83:4710-4714, 1986). In situ hybridization revealed dIRH transcripts in the ovaries of adult flies, in which the transcripts appeared to be synthesized by nurse cells for eventual storage as maternal RNA in the mature oocyte. Throughout embryogenesis, dIRH transcripts were ubiquitously expressed, although after midembryogenesis, higher levels were detected in the developing nervous system. Nervous system expression remained elevated throughout the larval stages and persisted in the adult, in which the cortex of the brain and ganglion cells were among the most prominently labeled tissues. In larvae, the imaginal disk cells exhibited comparatively high levels of dIRH mRNA expression. The broad distribution of dIRH mRNA in embryos and imaginal disks is compatible with a role for dIRH in anabolic processes required for cell growth. The apparently elevated expression of dIRH mRNA in nervous tissue during mid- and late embryogenesis coincides with a period of active neurite outgrowth and suggests that dIRH may be involved in this process.


Assuntos
Drosophila melanogaster/genética , Receptor de Insulina/genética , Transcrição Gênica , Animais , Clonagem Molecular , Enzimas de Restrição do DNA , Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Feminino , Genes , Masculino , Hibridização de Ácido Nucleico , Especificidade de Órgãos , RNA/genética , RNA Antissenso , RNA Mensageiro/antagonistas & inibidores , RNA Mensageiro/genética
9.
Mol Cell Biol ; 7(8): 2718-27, 1987 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-3118188

RESUMO

The insulin-binding and protein tyrosine kinase subunits of the Drosophila melanogaster insulin receptor homolog have been identified and characterized by using antipeptide antibodies elicited to the deduced amino acid sequence of the alpha and beta subunits of the human insulin receptor. In D. melanogaster embryos and cell lines, the insulin receptor contains insulin-binding alpha subunits of 110 or 120 kilodaltons (kDa), a 95-kDa beta subunit that is phosphorylated on tyrosine in response to insulin in intact cells and in vitro, and a 170-kDa protein that may be an incompletely processed receptor. All of the components are synthesized from a proreceptor, joined by disulfide bonds, and exposed on the cell surface. The beta subunit is recognized by an antipeptide antibody elicited to amino acids 1142 to 1162 of the human insulin proreceptor, and the alpha subunit is recognized by an antipeptide antibody elicited to amino acids 702 to 723 of the human proreceptor. Of the polypeptide ligands tested, only insulin reacts with the D. melanogaster receptor. Insulinlike growth factors type I and II, epidermal growth factor, and the silkworm insulinlike prothoracicotropic hormone are unable to stimulate autophosphorylation. Thus despite the evolutionary divergence of vertebrates and invertebrates, the essential features of the structure and intrinsic functions of the insulin receptor have been remarkably conserved.


Assuntos
Drosophila melanogaster/metabolismo , Proteínas Tirosina Quinases/metabolismo , Receptor de Insulina/metabolismo , Aminoácidos/análise , Animais , Anticorpos , Complexo Antígeno-Anticorpo , Linhagem Celular , Membrana Celular/metabolismo , Humanos , Ligantes , Substâncias Macromoleculares , Peso Molecular , Fosforilação
10.
Mol Cell Biol ; 10(2): 743-51, 1990 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-1688999

RESUMO

We report the functional expression of the mammalian muscle-adipocyte insulin-sensitive hexose transporter in Xenopus laevis oocytes. Oocytes microinjected with RNA synthesized in vitro showed enhanced hexose transport activity compared with uninjected controls. However, like the endogenous oocyte hexose transporter, activity was stimulated only twofold by 1 microM insulin. X. laevis oocytes injected with in vitro-synthesized RNA encoding the human insulin proreceptor expressed a functionally active insulin receptor that enhanced the insulin sensitivity of injected oocytes. This increase was not observed in oocytes expressing a mutant insulin receptor that lacked protein tyrosine kinase activity. In the presence of the coexpressed human insulin receptor, insulin induced a two- to threefold increase in hexose transport. The muscle-, brain-, and liver-type hexose carriers normally expressed in tissues with different responses to insulin exhibited the same insulin sensitivity when expressed in oocytes. This was observed whether or not the insulin signal was transduced through a coexpressed human insulin receptor or the endogenous oocyte insulin-like growth factor I receptor. We conclude that the expressed human insulin receptor is able to couple efficiently with preexisting postreceptor regulatory pathways in oocytes and that the regulation of hexose transport in these cells can be mediated through the combined actions of the expressed human insulin receptor and the endogenous oocyte insulin-like growth factor I receptor.


Assuntos
Encéfalo/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Genes , Insulina/farmacologia , Fígado/metabolismo , Proteínas de Transporte de Monossacarídeos/genética , Oócitos/metabolismo , Receptor de Insulina/genética , Transdução de Sinais/efeitos dos fármacos , Animais , Cinética , Microinjeções , Músculos/metabolismo , Oócitos/efeitos dos fármacos , Fosforilação , Proteínas Tirosina Quinases/metabolismo , RNA/administração & dosagem , RNA/genética , Ratos , Transcrição Gênica , Xenopus laevis
11.
Mol Cell Biol ; 11(7): 3407-18, 1991 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1675425

RESUMO

We show that D- but not L-hexoses modulate the accumulation of radioactive vinblastine in injected Xenopus laevis oocytes expressing the murine Mdr1b P-glycoprotein. We also show that X. laevis oocytes injected with RNA encoding the rat erythroid/brain glucose transport protein (GLUT1) and expressing the corresponding functional transporter exhibit a lower accumulation of [3H]vinblastine and show a greater capacity to extrude the drug than do control oocytes not expressing the rat GLUT1 protein. Cytochalasin B and phloretin, two inhibitors of the mammalian facilitative glucose transporters, can overcome the reduced drug accumulation conferred by expression of the rat GLUT1 protein in Xenopus oocytes but have no significant effect on the accumulation of drug by Xenopus oocytes expressing the mouse Mdr1b P-glycoprotein. These drugs also increase the accumulation of [3H]vinblastine in multidrug-resistant Chinese hamster ovary cells. Cytochalasin E, an analog of cytochalasin B that does not affect the activity of the facilitative glucose transporter, has no effect on the accumulation of vinblastine by multidrug-resistant Chinese hamster cells or by oocytes expressing either the mouse Mdr1b P-glycoprotein or the GLUT1 protein. In all three cases, the drug verapamil produces a profound effect on the cellular accumulation of vinblastine. Interestingly, although immunological analysis indicated the presence of massive amounts of P-glycoprotein in the multidrug-resistant cells, immunological and functional studies revealed only a minor increase in the expression of a hexose transporter-like protein in resistant versus drug-sensitive cells. Taken together, these results suggest the participation of the mammalian facilitative glucose transporter in the development of drug resistance.


Assuntos
Resistência a Medicamentos , Hexoses/farmacologia , Glicoproteínas de Membrana/genética , Proteínas de Transporte de Monossacarídeos/fisiologia , Oócitos/metabolismo , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Sequência de Aminoácidos , Animais , Anticorpos , Encéfalo/metabolismo , Linhagem Celular , Citocalasina B/farmacologia , Citocalasinas/farmacologia , Desoxiglucose/metabolismo , Glucose/metabolismo , Cinética , Glicoproteínas de Membrana/metabolismo , Camundongos , Dados de Sequência Molecular , Proteínas de Transporte de Monossacarídeos/genética , Oócitos/efeitos dos fármacos , Floretina/farmacologia , Ratos , Verapamil/farmacologia , Vimblastina/metabolismo , Xenopus laevis
12.
Diabetes ; 38(12): 1508-11, 1989 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-2555239

RESUMO

The actions of insulin are mediated by an integral plasma membrane protein, the insulin receptor. The processed receptor is a tetramer composed of two alpha-subunits that bind insulin and two beta-subunits that traverse the plasma membrane and are, in their cytosolic domains, protein tyrosine kinases. The insulin proreceptor cDNA has been cloned and its complete amino acid sequence deduced. The availability of cDNA permitted an analysis of both the role of protein tyrosine kinase activity in insulin action and the autophosphorylation sites that regulate kinase activity. The human cDNA probe has also been used to identify a putative Drosophila insulin receptor. This work is reviewed, and approaches that may be used to identify physiological substrates for the receptor kinase are suggested.


Assuntos
Receptor de Insulina/fisiologia , Animais , Membrana Celular/metabolismo , Humanos , Fosforilação , Proteínas Tirosina Quinases/metabolismo , Receptor de Insulina/genética , Receptor de Insulina/metabolismo
13.
Diabetes Care ; 13(3): 280-7, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2155093

RESUMO

Protein tyrosine kinase activity found in the beta-subunit of the insulin receptor provides a mechanism by which insulin binding on the outside of the cell transmits its signal across the plasma membrane into the cytosol. The autophosphorylation of the insulin receptor on tyrosyl residues activates the intrinsic tyrosine kinase of the receptor, rendering its ligand independent. Evidence suggests that phosphorylation of tyrosyl residues 1146, 1150, and 1151 in the kinase domain of the beta-subunit play a role in activation. Point mutations in the cytoplasmic portion of the beta-subunit confirm the above suggestions and indicate that additional sites are important for receptor function. We present methodology for overproducing the cytoplasmic domain of the receptor in the Baculovirus expression system. The protein, produced in insect cells and larvae, is soluble and fully active on autophosphorylation. Like the intact receptor, its autophosphorylation is intramolecular. Because greater than or equal to 10 mg of pure protein can be isolated from 10(10) insect cells infected with the recombinant Baculovirus encoding the human insulin-receptor kinase domain, sufficient enzyme is available for various studies, including physicochemical analysis. Isolation of beta-subunit defects found in the receptors of patients with various forms of diabetes mellitus also implicates the insulin-receptor kinase in insulin action. Finally, a potential model system for the genetic analysis of the insulin-insulin-receptor system with Drosophila melanogaster is noted. Conservation of the deduced amino acid sequence for both alpha- and beta-subunit sequences between humans and insects highlights the significance of this manner of signal transduction throughout nearly 1 billion years of evolution.


Assuntos
Proteínas Tirosina Quinases/metabolismo , Receptor de Insulina/metabolismo , Sequência de Aminoácidos , Animais , Humanos , Insulina/fisiologia , Dados de Sequência Molecular , Proteínas Tirosina Quinases/genética , Receptor de Insulina/genética
14.
FEBS Lett ; 231(2): 397-401, 1988 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-3360145

RESUMO

Protamine and poly(Lys) activate the protein tyrosine kinase of both the human placental insulin receptor and its purified recombinant cytoplasmic domain. Spermidine, poly(Arg) (average molecular mass 15 kDa), poly(Glu), Arg or Lys are not effective. Activation is stable, reversible, and optimal when the enzyme is preincubated with activator, divalent cation and ATP prior to the addition of exogenous protein substrates. The most striking feature of the activation is that it results in 20-30-fold stimulation of the kinase in the presence of 0.2-0.4 mM Mn2+ and induces equivalent activity in the presence of Mg2+ alone (0.4-4.0 mM). The activated protein tyrosine kinase has a specific activity (0.25-0.5 mumol/mg protein) that approaches that of well characterized protein serine kinases.


Assuntos
Magnésio/metabolismo , Manganês/metabolismo , Polilisina/farmacologia , Proteínas Tirosina Quinases/metabolismo , Receptor de Insulina/efeitos dos fármacos , Ativação Enzimática/efeitos dos fármacos , Humanos , Placenta/análise , Poliaminas/farmacologia , Receptor de Insulina/metabolismo , Proteínas Recombinantes/metabolismo
15.
Mol Cell Endocrinol ; 4(3): 167-82, 1976 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-174965

RESUMO

The protein kinase activities of a transplantable, insulin-producing hamster islet cell tumor were characterized using gel filtration, sucrose density gradient centrifugation and acrylamide gel electrophoresis. The post-microsomal supernatant fluid contains 70-80% of the protein kinase activity present in crude homogenates. A cAMP-dependent protein kinase, PK I (Mr 170,000), represents 25% of the soluble protein kinase activity assayed with protamine as substrate. It dissociates in the presence of cAMP into a cAMP-binding protein, R2 (Mr 90,000) and a catalytic subunit C (Mr 33,000). The dissociation induced by cAMP seems to be facilitated by the addition of Mg2+ and ATP. The regulatory subunit, R2, changes its gel filtration pattern in the presence of 0.5 M NaCl suggesting dissociation into a smaller subunit, R1 (Mr 44,000). By analogy with purified beef heart protein kinase (Erlichman et al., 1973) and skeletal muscle protein kinase, PK I. The presence in crude homogenates of a free cAMP-binding protein indistinguishable from the R2 derived by dissociation of PK I, suggests that PK I is partially dissociated in vivo. A cAMP-independent (casein) kinase (Mr 210,000) elutes with PK I on columns of Sepharose 6B. Another cAMP-independent protein kinase, PK II (Mr 88,000), is the predominatn form of soluble protein kinase accounting for approximately 75% of the soluble protein kinase activity detected using protaimine as substrate. This cAMP-independent protein kinase changes its gel filtration pattern in the presence of 0.5 M NaCl giving rise to a form which appears to have the same Mr (33,000) as the catalytic subunit of PK I. Studies comparing the catalytic subunit C of PK I with PK II and its salt-induced smaller molecular form demonstrate facile association of C with the cAMP-binding protein of purified bovine heart protein kinase to yield a hybrid holoenzyme, whereas PK II and its smaller form fail to recombine in this fashion. The 33,000 dalton forms derived from PK I (by cAMP) and PK II (by salt) also show different substrate specificities. It would appear, therefore, that pK II is a cAMP-independent protein kinase unrelated to PK I.


Assuntos
Adenoma de Células das Ilhotas Pancreáticas/enzimologia , Proteínas Quinases/metabolismo , Trifosfato de Adenosina/farmacologia , Animais , Sítios de Ligação , Cricetinae , AMP Cíclico/metabolismo , AMP Cíclico/farmacologia , Ativação Enzimática , Magnésio/farmacologia , Peso Molecular , Neoplasias Experimentais/enzimologia , Ligação Proteica , Proteínas Quinases/isolamento & purificação
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