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Stem cell maintenance in multilayered shoot apical meristems (SAMs) of plants requires strict regulation of cell growth and division. Exactly how the complex milieu of chemical and mechanical signals interact in the central region of the SAM to regulate cell division plane orientation is not well understood. In this paper, simulations using a newly developed multiscale computational model are combined with experimental studies to suggest and test three hypothesized mechanisms for the regulation of cell division plane orientation and the direction of anisotropic cell expansion in the corpus. Simulations predict that in the Apical corpus, WUSCHEL and cytokinin regulate the direction of anisotropic cell expansion, and cells divide according to tensile stress on the cell wall. In the Basal corpus, model simulations suggest dual roles for WUSCHEL and cytokinin in regulating both the direction of anisotropic cell expansion and cell division plane orientation. Simulation results are followed by a detailed analysis of changes in cell characteristics upon manipulation of WUSCHEL and cytokinin in experiments that support model predictions. Moreover, simulations predict that this layer-specific mechanism maintains both the experimentally observed shape and structure of the SAM as well as the distribution of WUSCHEL in the tissue. This provides an additional link between the roles of WUSCHEL, cytokinin, and mechanical stress in regulating SAM growth and proper stem cell maintenance in the SAM.
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Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Parede Celular/metabolismo , Simulação por Computador , Citocininas , Regulação da Expressão Gênica de Plantas , Proteínas de Homeodomínio/metabolismo , Meristema , Brotos de PlantaRESUMO
FTIR spectroscopy and Raman spectroscopy of biological analytes are increasingly explored as screening tools for early detection of cancer. In the present study, an integrated analysis of the FTIR and Raman spectra obtained from exfoliated cells is adopted to improve discrimination of normal, pre-cancerous and cancerous conditions. Multiple spectra were obtained from 13 normal, 13 pre-cancer and 10 cancer patients in both modes. Compared to normal patients, significant differences were observed at 1550, 1580, 1640, 2370, 2330, 2950-3000 and 3650-3750 cm-1 (FTIR) and 520, 640, 785, 827, 850, 935, 1003, 1175, 1311 cm-1 and 1606 cm-1 (Raman) vibrations of the other two. The increase in DNA, protein and lipid content with malignancy was more clearly elucidated by examining both spectra. Principal component analysis (PCA)-linear discriminant analysis (LDA) with 10-fold cross validation of the FTIR and Raman spectral data sets showed efficient discrimination between normal and pathological conditions while overlapping was seen between the two pathologies. The PCA-LDA model of the dual spectra yielded a classification accuracy of 98% in comparison with either FTIR (85%) or Raman (82%) in a spectrum-wise comparison. In the patient-wise approach (mean of all spectra from a patient), the overall classification efficiency was 73%, 80% and 87% for FTIR, Raman and integrated spectral approaches respectively. Moreover, the efficiency of the integrated FTIR-Raman PCA-LDA model as a prediction tool was tested to screen susceptible individuals (11 cigarette smokers) using the dual spectra acquired from these individuals. The study presents proof-of-concept for adopting a large-scale, follow-up trial of the approach for mass screening purposes.
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Detecção Precoce de Câncer/métodos , Mucosa Bucal , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Análise Espectral Raman/métodos , Células Cultivadas , Análise Discriminante , Células Epiteliais/patologia , Voluntários Saudáveis , Humanos , Mucosa Bucal/citologia , Mucosa Bucal/patologia , Análise de Componente PrincipalRESUMO
Supervised person re-identification (Re-ID) approaches are sensitive to label corrupted data, which is inevitable and generally ignored in the field of person Re-ID. In this paper, we propose a two-stage noise-tolerant paradigm (TSNT) for labeling corrupted person Re-ID. Specifically, at stage one, we present a self-refining strategy to separately train each network in TSNT by concentrating more on pure samples. These pure samples are progressively refurbished via mining the consistency between annotations and predictions. To enhance the tolerance of TSNT to noisy labels, at stage two, we employ a co-training strategy to collaboratively supervise the learning of the two networks. Concretely, a rectified cross-entropy loss is proposed to learn the mutual information from the peer network by assigning large weights to the refurbished reliable samples. Moreover, a noise-robust triplet loss is formulated for further improving the robustness of TSNT by increasing inter-class distances and reducing intra-class distances in the label-corrupted dataset, where a constraint condition for reliability discrimination is carefully designed to select reliable triplets. Extensive experiments demonstrate the superiority of TSNT, for instance, on the Market1501 dataset, our paradigm achieves 90.3% rank-1 accuracy (6.2% improvement over the state-of-the-art method) under noise ratio 20%.
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In many applications, we are constrained to learn classifiers from very limited data (few-shot classification). The task becomes even more challenging if it is also required to identify samples from unknown categories (open-set classification). Learning a good abstraction for a class with very few samples is extremely difficult, especially under open-set settings. As a result, open-set recognition has received limited attention in the few-shot setting. However, it is a critical task in many applications like environmental monitoring, where the number of labeled examples for each class is limited. Existing few-shot open-set recognition (FSOSR) methods rely on thresholding schemes, with some considering uniform probability for open-class samples. However, this approach is often inaccurate, especially for fine-grained categorization, and makes them highly sensitive to the choice of a threshold. To address these concerns, we propose Reconstructing Exemplar-based Few-shot Open-set ClaSsifier (ReFOCS). By using a novel exemplar reconstruction-based meta-learning strategy ReFOCS streamlines FSOSR eliminating the need for a carefully tuned threshold by learning to be self-aware of the openness of a sample. The exemplars, act as class representatives and can be either provided in the training dataset or estimated in the feature domain. By testing on a wide variety of datasets, we show ReFOCS to outperform multiple state-of-the-art methods.
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In plants, the robust maintenance of tissue structure is crucial to supporting its functionality. The multi-layered shoot apical meristem (SAM) of Arabidopsis, containing stem cells, is an approximately radially symmetric tissue whose shape and structure is maintained throughout the life of the plant. In this paper, a new biologically calibrated pseudo-three-dimensional (P3D) computational model of a longitudinal section of the SAM is developed. It includes anisotropic expansion and division of cells out of the cross-section plane, as well as representation of tension experienced by the SAM epidermis. Results from the experimentally calibrated P3D model provide new insights into maintenance of the structure of the SAM epidermal cell monolayer under tension and quantify dependence of epidermal and subepidermal cell anisotropy on the amount of tension. Moreover, the model simulations revealed that out-of-plane cell growth is important in offsetting cell crowding and regulating mechanical stresses experienced by tunica cells. Predictive model simulations show that tension-determined cell division plane orientation in the apical corpus may be regulating cell and tissue shape distributions needed for maintaining structure of the wild-type SAM. This suggests that cells' responses to local mechanical cues may serve as a mechanism to regulate cell- and tissue-scale patterning.
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Proteínas de Arabidopsis , Arabidopsis , Meristema/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Proliferação de Células , Regulação da Expressão Gênica de Plantas , Brotos de Planta/metabolismoRESUMO
The surgical needle insertion process is widely applied in medical interference. During the insertion process, the inhomogeneity and denseness of the soft tissues make it tough to detect the essential tissue damage, a rupture occurs that contains huge forces and material deformations. This study is very important, as all the above-mentioned factors are very significant for modern invasive surgery so that the success rate of the surgery can increase and the patient recovers smoothly. This investigation intends to perform minimally invasive surgical (MIS) procedures and reduce the living tissue damage while performing the biopsy, PCNL, etc. A fracture mechanics method was analyzed to create a needle insertion model which can estimate the needle insertion force during inset in tissue-like PVA gel. The force model was calculated by needle insertion experimentally, and also estimated the needle tip geometry, and diameter influences the fracture toughness. Validate exp. results with simulation results and other papers. It is observed that needle diameter has a significant effect on fracture toughness, whereas the insertion velocity has a slight impact on the fracture toughness. During the rotational needle insertion process, the winds-up of the gel occurs and the diameter of the hole was increasing with increased rpm. Maximum insertion force was noticed in the 27 G needle at 5 mm/s. The interaction function will be less at the maximum fracture development region.
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Materiais Biocompatíveis , Agulhas , Humanos , Fenômenos Mecânicos , Simulação por Computador , Procedimentos Cirúrgicos Minimamente InvasivosRESUMO
Improvement of cell migration by the nano-topographical modification of implant surface can directly or indirectly accelerate wound healing and osseointegration between bone and implant. Therefore, modification of the implant surface was done with TiO2 nanorod (NR) arrays to develop a more osseointegration-friendly implant in this study. Modulating the migration of a cell, adhered to a scaffold, by the variations of NR diameter, density and tip diameter in vitro is the primary objective of the study. The fluid structure interaction method was used, followed by the submodelling technique in this multiscale analysis. After completing a simulation over a global model, fluid structure interaction data was applied to the sub-scaffold finite element model to predict the mechanical response over cells at the cell-substrate interface. Special focus was given to strain energy density at the cell interface as a response parameter due to its direct correlation with the migration of an adherent cell. The results showed a huge rise in strain energy density after the addition of NRs on the scaffold surface. It also highlighted that variation in NR density plays a more effective role than the variation in NR diameter to control cell migration over a substrate. However, the effect of NR diameter becomes insignificant when the NR tip was considered. The findings of this study could be used to determine the best nanostructure parameters for better osseointegration.
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Nanotubos , Titânio , Titânio/química , Nanotubos/química , Osseointegração , Próteses e ImplantesRESUMO
Targeted delivery of site-specific therapeutic agents is an effective strategy for osteoarthritis treatment. The lack of blood vessels in cartilage makes it difficult to deliver therapeutic agents like peptides to the defect area. Therefore, nucleus-targeting zwitterionic carbon nano-dots (CDs) have immense potential as a delivery vehicle for effective peptide delivery to the cytoplasm as well as nucleus. In the present study, nucleus-targeting zwitterionic CDs have been synthesized as delivery vehicle for peptides while also working as nano-agents towards optical monitoring of cartilage healing. The functional groups of zwitterion CDs were introduced by a single-step microwave assisted oxidation procedure followed by COL II peptide conjugation derived from Capra auricular cartilage through NHS/EDC coupling. The peptide-conjugated CDs (PCDs) allows cytoplasmic uptake within a short period of time (â¼30 m) followed by translocation to nucleus after â¼24 h. Moreover, multicolor fluorescence of PCDs improves (blue, green, and read channel) its sensitivity as an optical code providing a compelling solution towards enhanced non-invasive tracking system with multifunctional properties. The PCDs-based delivery system developed in this study has exhibited superior ability to induce ex-vivo chondrogenic differentiation of ADMSCs as compared to bare CDs. For assessment of cartilage regeneration potential, pluronic F-127 based PCDs hydrogel was injected to rabbit auricular cartilage defects and potential healing was observed after 60 days. Therefore, the results confirm that PCDs could be an ideal alternate for multimodal therapeutic agents.
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Extrusion-based bioprinting is an enabling biofabrication technique that is used to create heterogeneous tissue constructs according to patient-specific geometries and compositions. The optimization of bioinks as per requirements for specific tissue applications is an essential exercise in ensuring clinical translation of the bioprinting technologies. Most notably, optimum hydrogel polymer concentrations are required to ensure adequate mechanical properties of bioprinted constructs without causing significant shear stresses on cells. However, experimental iterations are often tedious for optimizing the bioink properties. In this work, a nonlinear finite element modeling approach has been undertaken to determine the effect of different bioink parameters such as composition, concentration on the range of stresses being experienced by the cells in the bioprinted construct. The stress distribution of the cells at different parts of the constructs has also been modeled. It is found that both bioink chemical compositions and concentrations can substantially alter the stress effects experienced by the cells. Concentrated regions of softer cells near pore regions were found to increase stress concentrations by almost three times compared with stress generated in cells away from the pores. The study provides a method for rapid optimization of bioinks, design of bioprinted constructs, as well as toolpath plans for fabricating constructs with homogenous properties.
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The articular disc reduces the stress distribution from the mandible to fossa. In total temporomandibular joint (TMJ) replacement, the implant is required to reduce the stress on fossa implant. Current studies lack standard and optimized parameters for the cylindrical dome on Christensen TMJ implant collar. This study briefed a novel TMJ implant head design and investigates the biomechanical behaviour by considering the articular disc. The radius of the head was varied with the height of the cylinder height to obtain the design of the experiment and the stress distribution was compared with an intact mandible-articular disc model by considering the viscoelastic property of the TMJ disc. The model was simulated at three different angles: 20°, 0° and -20° in the mediolateral direction to simulate the manducation. FEA analysis showed high stresses at the circular heads, and high strength is achieved with increased implant cylinder length and diameter. The results also showed a stress reduction of 50% on the fossa from the mandible. Hence, the newly designed head and suggested modifications may be used as a reference for further clinical improvement of Christensen TMJ as well as other TMJ implants.
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Prótese Articular , Côndilo Mandibular , Fenômenos Biomecânicos , Análise de Elementos Finitos , Articulação Temporomandibular/cirurgia , Disco da Articulação TemporomandibularRESUMO
Shoot apical meristems (SAMs) of higher plants harbor stem-cell niches. The cells of the stem-cell niche are organized into spatial domains of distinct function and cell behaviors. A coordinated interplay between cell growth dynamics and changes in gene expression is critical to ensure stem-cell homeostasis and organ differentiation. Exploring the causal relationships between cell growth patterns and gene expression dynamics requires quantitative methods to analyze cell behaviors from time-lapse imagery. Although technical breakthroughs in live-imaging methods have revealed spatio-temporal dynamics of SAM-cell growth patterns, robust computational methods for cell segmentation and automated tracking of cells have not been developed. Here we present a local graph matching-based method for automated-tracking of cells and cell divisions of SAMs of Arabidopsis thaliana. The cells of the SAM are tightly clustered in space which poses a unique challenge in computing spatio-temporal correspondences of cells. The local graph-matching principle efficiently exploits the geometric structure and topology of the relative positions of cells in obtaining spatio-temporal correspondences. The tracker integrates information across multiple slices in which a cell may be properly imaged, thus providing robustness to cell tracking in noisy live-imaging datasets. By relying on the local geometry and topology, the method is able to track cells in areas of high curvature such as regions of primordial outgrowth. The cell tracker not only computes the correspondences of cells across spatio-temporal scale, but it also detects cell division events, and identifies daughter cells upon divisions, thus allowing automated estimation of cell lineages from images captured over a period of 72 h. The method presented here should enable quantitative analysis of cell growth patterns and thus facilitating the development of in silico models for SAM growth.
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Arabidopsis/crescimento & desenvolvimento , Linhagem da Célula , Reconhecimento Automatizado de Padrão , Brotos de Planta/citologia , Células-Tronco/citologia , Divisão Celular , Meristema/citologia , Microscopia Confocal , Modelos TeóricosRESUMO
Prior works on text-based video moment localization focus on temporally grounding the textual query in an untrimmed video. These works assume that the relevant video is already known and attempt to localize the moment on that relevant video only. Different from such works, we relax this assumption and address the task of localizing moments in a corpus of videos for a given sentence query. This task poses a unique challenge as the system is required to perform: 2) retrieval of the relevant video where only a segment of the video corresponds with the queried sentence, 2) temporal localization of moment in the relevant video based on sentence query. Towards overcoming this challenge, we propose Hierarchical Moment Alignment Network (HMAN) which learns an effective joint embedding space for moments and sentences. In addition to learning subtle differences between intra-video moments, HMAN focuses on distinguishing inter-video global semantic concepts based on sentence queries. Qualitative and quantitative results on three benchmark text-based video moment retrieval datasets - Charades-STA, DiDeMo, and ActivityNet Captions - demonstrate that our method achieves promising performance on the proposed task of temporal localization of moments in a corpus of videos.
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Most person re-identification methods, being supervised techniques, suffer from the burden of massive annotation requirement. Unsupervised methods overcome this need for labeled data, but perform poorly compared to the supervised alternatives. In order to cope with this issue, we introduce the problem of learning person re-identification models from videos with weak supervision. The weak nature of the supervision arises from the requirement of video-level labels, i.e. person identities who appear in the video, in contrast to the more precise frame-level annotations. Towards this goal, we propose a multiple instance attention learning framework for person re-identification using such video-level labels. Specifically, we first cast the video person re-identification task into a multiple instance learning setting, in which person images in a video are collected into a bag. The relations between videos with similar labels can be utilized to identify persons, on top of that, we introduce a co-person attention mechanism which mines the similarity correlations between videos with person identities in common. The attention weights are obtained based on all person images instead of person tracklets in a video, making our learned model less affected by noisy annotations. Extensive experiments demonstrate the superiority of the proposed method over the related methods on two weakly labeled person re-identification datasets.
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Activity recognition is a challenging problem with many practical applications. In addition to the visual features, recent approaches have benefited from the use of context, e.g., inter-relationships among the activities and objects. However, these approaches require data to be labeled, entirely available beforehand, and not designed to be updated continuously, which make them unsuitable for surveillance applications. In contrast, we propose a continuous-learning framework for context-aware activity recognition from unlabeled video, which has two distinct advantages over existing methods. First, it employs a novel active-learning technique that not only exploits the informativeness of the individual activities but also utilizes their contextual information during query selection; this leads to significant reduction in expensive manual annotation effort. Second, the learned models can be adapted online as more data is available. We formulate a conditional random field model that encodes the context and devise an information-theoretic approach that utilizes entropy and mutual information of the nodes to compute the set of most informative queries, which are labeled by a human. These labels are combined with graphical inference techniques for incremental updates. We provide a theoretical formulation of the active learning framework with an analytic solution. Experiments on six challenging datasets demonstrate that our framework achieves superior performance with significantly less manual labeling.
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Fabrication of hollow channels with user-defined dimensions and patterns inside viscoelastic, gel-type materials is required for several applications, especially in biomedical engineering domain. These include objectives of obtaining vascularized tissues and enclosed or subsurface microfluidic devices. However, presently there is no suitable manufacturing technology that can create such channels and networks in a gel structure. The advent of three-dimensional bioprinting has opened new possibilities for fabricating structures with complex geometries. However, application of this technique to fabricate internal hollow channels in viscoelastic material has not been yet explored to a great extent. In this article, we present the theoretical modeling/background of a proposed manufacturing paradigm through which hollow channels can be conveniently fabricated inside a gel structure. We propose that a tip connected to a robotic arm can be moved in X-, Y-, and Z-axis as per the desired design. The tip can be moved by a magnet or mechanical force. If the tip is further trailed with porous tube and moved inside the viscoelastic material, corresponding internal channels can be fabricated. To achieve this, however, force modeling to understand the forces that will be required to move the tip inside viscoelastic material should be known and understood. Therefore, in our first attempt, we developed the computational force modeling of the tip movement inside gels with different viscoelastic properties to create the channels.
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Desenho de Equipamento/métodos , Géis/química , Engenharia Tecidual/instrumentação , Análise de Elementos Finitos , Agulhas , Porosidade , Impressão TridimensionalRESUMO
Pattern recognition in video is a challenging task because of the multitude of spatio-temporal variations that occur in different videos capturing the exact same event. While traditional pattern-theoretic approaches account for the spatial changes that occur due to lighting and pose, very little has been done to address the effect of temporal rate changes in the executions of an event. In this paper, we provide a systematic model-based approach to learn the nature of such temporal variations (time warps) while simultaneously allowing for the spatial variations in the descriptors. We illustrate our approach for the problem of action recognition and provide experimental justification for the importance of accounting for rate variations in action recognition. The model is composed of a nominal activity trajectory and a function space capturing the probability distribution of activity-specific time warping transformations. We use the square-root parameterization of time warps to derive geodesics, distance measures, and probability distributions on the space of time warping functions. We then design a Bayesian algorithm which treats the execution rate function as a nuisance variable and integrates it out using Monte Carlo sampling, to generate estimates of class posteriors. This approach allows us to learn the space of time warps for each activity while simultaneously capturing other intra- and interclass variations. Next, we discuss a special case of this approach which assumes a uniform distribution on the space of time warping functions and show how computationally efficient inference algorithms may be derived for this special case. We discuss the relative advantages and disadvantages of both approaches and show their efficacy using experiments on gait-based person identification and activity recognition.
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Algoritmos , Modelos Estatísticos , Movimento/fisiologia , Reconhecimento Automatizado de Padrão/métodos , Antropometria , Teorema de Bayes , Marcha/fisiologia , Humanos , Método de Monte Carlo , Gravação em VídeoRESUMO
In this paper, we present a novel approach to find informative and anomalous samples in videos exploiting the concept of typicality from information theory. In most video analysis tasks, selection of the most informative samples from a huge pool of training data in order to learn a good recognition model is an important problem. Furthermore, it is also useful to reduce the annotation cost as it is time-consuming to annotate unlabeled samples. Typicality is a simple and powerful technique which can be applied to compress the training data to learn a good classification model. In a continuous video clip, an activity shares a strong correlation with its previous activities. We assume that the activity samples that appear in a video form a Markov chain. We explicitly show how typicality can be utilized in this scenario. We compute an atypical score for a sample using typicality and the Markovian property, which can be applied to two challenging vision problems-(a) sample selection for learning activity recognition models, and (b) anomaly detection. In the first case, our approach leads to a significant reduction of manual labeling cost while achieving similar or better recognition performance compared to a model trained with the entire training set. For the latter case, the atypical score has been exploited in identifying anomalous activities in videos where our results demonstrate the effectiveness of the proposed framework over other recent strategies.
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With advanced image journaling tools, one can easily alter the semantic meaning of an image by exploiting certain manipulation techniques such as copy clone, object splicing, and removal, which mislead the viewers. In contrast, the identification of these manipulations becomes a very challenging task as manipulated regions are not visually apparent. This paper proposes a high-confidence manipulation localization architecture that utilizes resampling features, long short-term memory (LSTM) cells, and an encoder-decoder network to segment out manipulated regions from non-manipulated ones. Resampling features are used to capture artifacts, such as JPEG quality loss, upsampling, downsampling, rotation, and shearing. The proposed network exploits larger receptive fields (spatial maps) and frequency-domain correlation to analyze the discriminative characteristics between the manipulated and non-manipulated regions by incorporating the encoder and LSTM network. Finally, the decoder network learns the mapping from low-resolution feature maps to pixel-wise predictions for image tamper localization. With the predicted mask provided by the final layer (softmax) of the proposed architecture, end-to-end training is performed to learn the network parameters through back-propagation using the ground-truth masks. Furthermore, a large image splicing dataset is introduced to guide the training process. The proposed method is capable of localizing image manipulations at the pixel level with high precision, which is demonstrated through rigorous experimentation on three diverse datasets.
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Synthesis of strontium-doped hydroxyapatite from Mercenaria clam shells has been carried out by hydrothermal method. The doping of bioceramic, processed from biogenic resources is mostly unexplored. The objective is to understand the effect of strontium (Sr) incorporation on phase stability, sintering behaviour, mechanical properties and cytotoxicity of hydroxyapatite (HAp) derived from clam shells. The different molar concentrations of Sr, varies from 10, 30, 50, 70% of Ca, were substituted into the HAp. The synthesized powders were sintered at 1200⯰C in air. The as synthesized powders and sintered specimens were characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and high resolution transmission electron microscopy. The crystallite size and cell parameters of sintered specimens were analyzed from XRD. The XRD of hydrothermally synthesized powders mostly matched with HAp with slight shifting due to Sr doping. However, some distinct Sr based compounds were also observed where Sr substitution is more that 50% of Ca. The XRD of sintered specimen showed increasing ß-tricalcium phosphate (ß-TCP) phase with Sr substitution. The sintered density of solid samples gradually increased from 3.04â¯g/cc to 3.50â¯g/cc and surface energy decreased with increasing Sr substitution. Similarly, microhardness, fracture toughness and nanohardness of solid samples found to be enhanced with Sr substitution. The elastic modulus gradually increased from 130 to 137â¯GPa for HAp and Sr substituted HAp (70% of Ca). The in vitro cytotoxicity of sintered specimen against mouse osteoblast cell line showed that all the samples were nontoxic. However cell proliferation found low for the solid samples containing more than 50% Sr substitution.
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Exoesqueleto/química , Durapatita/química , Durapatita/síntese química , Fenômenos Mecânicos , Mercenaria/anatomia & histologia , Estrôncio/química , Células 3T3 , Animais , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/toxicidade , Técnicas de Química Sintética , Durapatita/toxicidade , Camundongos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Transição de Fase , Propriedades de SuperfícieRESUMO
In this paper, we show how to estimate, accurately and efficiently, the 3D motion of a rigid object and time-varying lighting in a dynamic scene. This is achieved in an inverse compositional tracking framework with a novel warping function that involves a 2D --> 3D --> 2D transformation. This also allows us to extend traditional two frame inverse compositional tracking to a sequence of frames, leading to even higher computational savings. We prove the theoretical convergence of this method and show that it leads to significant reduction in computational burden. Experimental analysis on multiple video sequences shows impressive speed-up over existing methods while retaining a high level of accuracy.