RESUMO
Considerable progress has recently been achieved in understanding molecular medicine in general and vell molecular biology in particular. Molecular medicine is a scientific discipline studying man under normal condition and pathological changes at the cellular-molecular level with reference to gene activity and function of protein mediators responsible for delivery of information to various organs or systems of the body and cell-to-cell interaction.
Assuntos
Benzamidas/administração & dosagem , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Neoplasias Gastrointestinais , Tumores do Estroma Gastrointestinal , Piperazinas/administração & dosagem , Pirimidinas/administração & dosagem , Idoso , Protocolos Antineoplásicos , Biomarcadores Tumorais/genética , Feminino , Neoplasias Gastrointestinais/diagnóstico , Neoplasias Gastrointestinais/genética , Neoplasias Gastrointestinais/terapia , Tumores do Estroma Gastrointestinal/diagnóstico , Tumores do Estroma Gastrointestinal/genética , Tumores do Estroma Gastrointestinal/terapia , Trato Gastrointestinal/patologia , Testes Genéticos/métodos , Humanos , Mesilato de Imatinib , Masculino , Pessoa de Meia-Idade , Técnicas de Diagnóstico Molecular/métodos , Prognóstico , Inibidores de Proteínas Quinases/administração & dosagem , Proteínas Quinases/genética , Resultado do TratamentoRESUMO
The study of the model of superinvasive opisthorchiasis (SO) in Syrian hamsters and autopsy material obtained from patients with SO, it was shown that during the early period of pulmonary disease, the lungs underwent changes developing on the basis of delayed-type hypersensitivity reactions. In the chronic phase of SO, exudative and predominant productive reactions were revealed leading to the interstitial lung disease, fibrosing alveolitis. In the heart in SO, eosinophilic cell myocarditis was found with subsequent diffuse cardiosclerosis. In the testes, sclerotic processes with atrophy of glandulocytes (Leydig cells) and sustentocytes (Sertoli cells) were detected. The structural changes in the lungs, heart and testes were induced by parasite metabolites, that are deposited in tissues in the form of granules and conglomerates.
Assuntos
Opistorquíase/patologia , Opisthorchis , Adulto , Animais , Cricetinae , Modelos Animais de Doenças , Humanos , Hipersensibilidade Tardia/parasitologia , Hipersensibilidade Tardia/patologia , Pulmão/parasitologia , Pulmão/patologia , Masculino , Miocárdio/patologia , Testículo/parasitologia , Testículo/patologia , Adulto JovemRESUMO
Superinvasion opisthorchiasis is characterized by host growth retardation and weight reduction, which are due to the cleptoeffect of Opisthorchis felineus; metabolic disturbances, proliferative processes, accelerated collagen biosynthesis and biodegradation in the organs reflect parasite-host interactions as components of the system. The productive reactions of visceral pathology are initiated by the parasite's metabolites located in the liver, lung, heart, and testes.
Assuntos
Fígado/patologia , Pulmão/patologia , Opistorquíase , Opisthorchis/crescimento & desenvolvimento , Phodopus/parasitologia , Testículo/patologia , Adolescente , Adulto , Animais , Antropometria , Estatura , Estudos de Casos e Controles , Cricetinae , Coração/parasitologia , Histocitoquímica , Interações Hospedeiro-Parasita , Humanos , Fígado/parasitologia , Pulmão/parasitologia , Masculino , Malondialdeído/análise , Metacercárias/crescimento & desenvolvimento , Microscopia Eletrônica de Transmissão , Opistorquíase/metabolismo , Opistorquíase/parasitologia , Opistorquíase/patologia , Opistorquíase/fisiopatologia , Opistorquíase/veterinária , Federação Russa , Superóxido Dismutase/análise , Testículo/parasitologiaRESUMO
Changes in the liver where Opisthorchis felines (Rivolta, 1884) parasitizes were traced by light and transmission electron microscopies on experimental models (gold hamsters) of acute and chronic opisthorchiasis. Regenerative processes, such as hepatic epithelial mitotic indices, binucleated, hypertrophic hepatocytes, were determined; the composition of infiltrates was identified; fibroplastic changes and hepatocytic apoptotic index were traced. The progression/regression (P/R) ratio was used to detect atrophy of a hepatic parenchymatous component (hepatocytes) in different periods (from 3 to 120 days) of invasion. In opisthorchiasis, proliferation of committed hepatocytes was first found on day 10 of invasion. Oval (committed) cells are differentiated mainly to cholangiocytes, followed by the formation of ductular structures; the committed cells of the liver may be simultaneously a source of programmed hepatocellular differentiation; however, the newly formed hepatocytes are solitary amongst them in opisthorchiasis.
Assuntos
Fígado/parasitologia , Fígado/ultraestrutura , Opistorquíase/patologia , Opisthorchis , Animais , Apoptose , Diferenciação Celular , Cricetinae , Modelos Animais de Doenças , Hepatócitos/parasitologia , Hepatócitos/ultraestrutura , Regeneração Hepática , Masculino , Microscopia Eletrônica de Transmissão , Índice Mitótico , Fatores de TempoRESUMO
Light, electron microscopy, polymerase chain reaction (PCR), and immunohistochemistry were used to reveal the proliferative, alterative processes of hepatic parenchymal cells, P 53, K-ras, B-raf, c-Kit, p 16, APC, immunoreactive proteins Ki-67, cytokeratins 9, 14, 20, EMA, c-erb B-2, CD-117, mutated p 53 and bcl-2 genes. P53, K-ras, B-raf, c-Kit, and p 16 gene mutations were detected in the liver of animals with experimental superinvasive opisthorchiasis (SO) and cholangiocellular carcinoma (CCC). Mutations of these genes were found in the plasma samples taken from SO patients. The CCC tissue from SO patients displayed expression of the following genes: p 53 (100.0%), p 53+B-raf (50.0%), K-ras+c-Kit (33.3%), K-ras+B-rafat more than 5 cm from the tumor in 2 cases. Following 2 years, target therapy (canglait, megamin, immunomodulators) leveled mutations in 91.25% of the patients with SO.