The Change in Winter Wheat Response to Deoxynivalenol and Fusarium Head Blight Through Technological and Agronomic Progress.

Fusarium head blight (FHB) in wheat causes yield loss, quality reduction, and mycotoxin contamination in temperate wheat production areas worldwide. The objective of this study was to quantify the progress of agronomic and FHB management strategies over the past two decades in FHB suppression and agronomic performance of winter wheat in environments favorable for FHB. Field experiments were conducted in environments typical of FHB epidemics to compare common agronomic and FHB management practices used in the 1996 era compared with those used in 2016. The experiments included a comparison of three different nitrogen (N) fertilizer application rates and six old (1996-era) and new (modern-era) winter wheat cultivars representing combinations of susceptibility and era to FHB, with and without a fungicide applied at flowering (pydiflumetofen + propiconazole). To mimic environments favorable for infection (similar to 1996 in Ontario, Canada), plots were challenged at 50% anthesis with F. graminearum macroconidia suspension followed by mist irrigation. The modern management strategy of using moderately resistant cultivars, a fungicide applied at flowering, and a high rate of N fertilizer reduced total deoxynivalenol by 67%, reduced Fusarium-damaged kernels by 49%, reduced FHB index by 86%, increased grain test weight by 11%, and increased grain yield by 31% compared with the standard management practice of seeding highly susceptible cultivars with no fungicide and a lower rate of N fertilizer recommended in the 1996 era. This study enabled a published economic assessment of the return on investment for the improvements in cultivars, fungicide, and N fertilizer applications since 1996.

Early Detection and Mitigation of Resistance to Bt Maize by Western Corn Rootworm (Coleoptera: Chrysomelidae).

Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges. Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, and more general concerns around Bt crops that produce less than a high-dose of Bt toxin, have caused uncertainty around current IRM protocols. More than 15 years of experience with IRM has shown that high-dose and refuge-based IRM is not applicable to Bt crops that produce less than a high-dose. Adaptive IRM approaches and pro-active, integrated IRM-pest management strategies are needed and should be in place before release of new technologies that produce less than a high-dose. We suggest changes in IRM strategies to preserve the utility of corn rootworm Bt maize by 1) targeting local resistance management earlier in the sequence of responses to resistance and 2) developing area-wide criteria to address widespread economic losses. We also favor consideration of policies and programs to counteract economic forces that are contributing to rapid resistance evolution.

Quantifying Neonicotinoid Insecticide Residues Escaping during Maize Planting with Vacuum Planters.

Neonicotinoid residues escaping in vacuum-planter exhaust during maize planting were measured in 25 fields in southwestern Ontario in 2013-2014 using filter bags to collect planter exhaust dust and horizontal and vertical sticky traps to collect planter operation-generated dust. Atrazine residues were used to differentiate between neonicotinoid residues originating from seed or from disturbed soil. Recovery rates of seed-applied neonicotinoids in exhaust were 0.014 and 0.365% in 2013 and 2014, respectively, calculated on the basis of neonicotinoid concentrations in preplant soil and seed application rates. Neonicotinoid exhaust emission rates were 0.0036 and 0.1104 g/ha for 2013 and 2014, respectively, with 99.9472 and 99.7820% originating from treated seed in 2013 and 2014, respectively, calculated on the basis of the atrazine marker. Rates of recovery of seed-applied neonicotinoid residues by exhaust filter bags were 0.015 and 0.437% for 2013 and 2014, respectively. Neonicotinoid residues captured on horizontal and vertical traps were 1.10 ng/cm2 (0.1104 g/ha) and 1.45 ng/cm2 (0.0029 g/ha), respectively, with 92.31 and 93.03% originating from treated seed, respectively, representing 0.3896% of the original active ingredient applied to the seed planted. Exposure to pollinators can be best reduced by strategies to keep active ingredient on the seed, below the soil surface, and in the field where applied.

Susceptibility of Aphelinus certus (Hymenoptera: Aphelinidae) to neonicotinoid seed treatments used for soybean pest management.

Soybean aphid is an economic pest of soybean in North America. Currently, management of soybean aphid is achieved through the use of foliar- and seed-applied insecticides. However, natural enemies play an important role in regulating soybean aphid populations, and may be adversely affected by insecticides. The effects of imidacloprid and thiamethoxam seed treatments on the soybean aphid parasitoid, Aphelinus certus Yasnosh, were examined using a tritrophic bioassay. A. certus was able to parasitize soybean aphids feeding on imidacloprid- and thiamethoxam-treated plants 5 and 6 wk after planting, respectively. However, up to 10 wk after planting, overall parasitism rates were reduced by 69-88% compared with the control. Therefore, neonicotinoid seed treatments may reduce the effectiveness of A. certus as a natural enemy of soybean aphid in seed-treated crops.

Susceptibility and field exposure of Striacosta albicosta (Lepidoptera: Noctuidae) eggs and larvae in Ontario, Canada to four insecticides.

BACKGROUND: Striacosta albicosta Smith (Lepidoptera: Noctuidae) is a primary pest of corn, Zea mays L., in the Great Lakes region, causing yield loss and exacerbating mycotoxin contamination of grain. Foliar insecticides are currently used to manage S. albicosta; however, the toxicity and residual activity of these insecticides against S. albicosta are unknown. Laboratory and field bioassays were conducted to determine the susceptibility and period of in-field efficacy provided by chlorantraniliprole, lambda-cyhalothrin, spinetoram, and methoxyfenozide against S. albicosta. Bioassay data were used to simulate management scenarios. RESULTS: For all insecticides tested, 1st instars were highly susceptible to the recommended field application rates and were >3-fold more susceptible to insecticides than 3rd instars. Insecticide activity decreased after application for all insecticides, with chlorantraniliprole having the longest residual activity. In simulated management scenarios where an insecticide was applied at or below the recommended 5% egg mass threshold with additional oviposition, methoxyfenozide application resulted in greater larval survival 14 days after application (DAA) than the other insecticides tested. In scenarios where insecticides were applied 7 days before threshold was reached, all insecticides resulted in larval survival. CONCLUSION: These data demonstrate that chlorantraniliprole, lambda-cyhalothrin and spinetoram, applied in conjunction with monitoring, provide effective control of S. albicosta larvae for 10-14 days, whereas methoxyfenozide provides effective control for less than 7 days. © 2022 Society of Chemical Industry.

Sublethal effects of Cry 1F Bt corn and clothianidin on black cutworm (Lepidoptera: Noctuidae) larval development.

Black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), is an occasional pest of maize (corn), Zea mays L., that may cause severe stand losses and injury to corn seedlings. The efficacy of the neonicotinoid seed treatment clothianidin at two commercially available rates and their interaction with a transgenic corn hybrid (Bt corn), trait expressing the Bacillus thuringiensis variety aizawai insecticidal toxin Cry 1Fa2, against black cutworm larvae was investigated. Clothianidin at a rate of 25 mg kernel(-1) on Bt corn increased larval mortality and reduced larval weight gains additively. In contrast, weights of larvae fed non-Bt corn seedlings treated with clothianidin at a rate of 25 mg kernel(-1) increased significantly, suggesting either compensatory overconsumption, hormesis, or hormoligosis. Both Bt corn alone and clothianidin at a rate of 125 mg kernel(-1) applied to non-Bt corn seedlings caused increased mortality and reduced larval weight gains. In two field trials, plots planted with Bt corn hybrids consistently had the highest plant populations and yields, regardless of whether they were treated with clothianidin at the lower commercial rate of 25 mg kernel(-1) The use of Bt corn alone or in combination with the low rate of clothianidin (25 mg kernel(-1)) seems suitable as a means of suppressing black cutworm in no-tillage cornfields, although rescue treatments may still be necessary under severe infestations. Clothianidin alone at the low rate of 25 mg kernel(-1) is not recommended for black cutworm control until further studies of its effects on larval physiology and field performance have been completed.

Relationship between Mycotoxin Content in Winter Wheat Grain and Aspirated Dust Collected during Harvest and after Storage.

A total of 323 paired grain and grain dust samples (particle size <1650 µm) were collected from combines at harvest (56%), on-farm bins (28%), and experimental minibins seeded with an ochratoxin A (OTA)/Penicillium verrucosum hot spots (15%) of which >98% were soft red winter wheat. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to detect 21 mycotoxins, including deoxynivalenol (DON) and its plant-conjugated form, deoxynivalenol 3-ß-d-glucoside (DON 3-Glc). Except for DON 3-Glc, all mycotoxin concentrations found in grain dust were higher than in grain (p < 0.0030). Pearson correlation coefficients and two-variable regression show a significant (p < 0.0001) linear relationship between the mycotoxin content in grain and that in grain dust with 19 toxins. In only five mycotoxins (DON, OTA, ochratoxin B, citrinin, and enniatin A1), more than 82% of the variation in the data is explained by the two-variable regression model. Because of its higher mean concentration and detection frequency, only DON produced a strong relationship (p < 0.0001, r 2 = 0.949) with low root-mean-square error (RMSE) (293.41 ng/g). The results suggest that modeling levels in grain based upon levels in grain dust can be used to estimate DON in grain bulk.

Fugitive Dust During Planting of Canola with an Air Seeder as a Source of Environmental Contamination for Pesticides Applied on Seed: A Case Study.

The dispersion of clothianidin from treated seeds was studied in a commercial winter canola field. During planting, using a John Deere 1890 single disk air seeder, a proportion, an estimated 14.2 ± 2.9% (mean ± standard error), of the clothianidin that was applied to the seed escaped into the atmosphere from the seeder's exhaust. We suggest that this source of environmental contamination may be the main contributor that explains the off-target detection of neonicotinoid residues in soils and water near canola plantings better than movement from seed after it is placed in the soil, which is often proposed in the literature. Environ Toxicol Chem 2020;39:2420-2423. © 2020 SETAC.

Quantifying Early-Season Pest Injury and Yield Protection of Insecticide Seed Treatments in Corn and Soybean Production in Ontario, Canada.

A 4-yr study was conducted comparing the efficacy and value of fungicide-only (FST), neonicotinoid insecticide + fungicide (NST), and diamide insecticide + fungicide (DST) seed treatments for commercial corn Zea mays L. and soybean Glycines max (L.) Merr. production in Ontario, Canada. Plant stand, plant vigor, above- and below-ground insect injury, and yield were assessed on 160 field-scale experiments. Experiments also assessed early-season insect incidence and abundance using newly legislated thresholds for NST use in Ontario and in-season destructive sampling. Wireworms (Coleoptera: Elateridae) and white grubs (Coleoptera: Scarabeidae) were frequently observed at experimental sites; however, thresholds were rarely met and injury levels rarely led to yield loss. Of 129 and 31 corn and soybean sites, 8 and 6%, respectively, had a positive yield response to NST use. Across all sites, yield response of 0.1 and -0.05 Mg ha-1 was observed with NST use in corn and soybean, respectively; however, the costs associated with NST use were recovered at only 48 and 23% of corn and soybean sites, respectively, based on average grain prices and yields during the study. Infrequent incidence of economic injury and the absence of a consistent yield response to NST and DSTs throughout the 4 yr of the study indicate that widespread use of seed-applied insecticides in corn and soybean is unlikely to provide benefit to producers. These data highlight an opportunity for reducing input costs, environmental loading, and nontarget effects without adverse outcomes for Ontario producers.

The Effect of Simulated Lepidopteran Ear Feeding Injury on Mycotoxin Accumulation in Grain Corn (Poales: Poaceae).

Fusarium graminearum Schwabe (Hypocreales: Nectriaceae) and Fusarium verticillioides (Saccardo) (Hypocreales: Nectriaceae) Nirenberg infection results in accumulation of deoxynivalenol (DON), zearalenone (ZON), and fumonisin (FBs) mycotoxins in infected corn, Zea mays L. Lepidopteran insect feeding may exacerbate fungal infection by providing entry points on the ear resulting in increased mycotoxin contamination of grain. The objective of the current study was to simulate different types and severity levels (extent of injury) of lepidopteran injury to corn ears at different stages of ear development and its effect on mycotoxin accumulation in grain corn. Field experiments were conducted under conditions favorable for F. graminearum development where insect injury was simulated to corn ears and inoculated with F. graminearum. All simulated injury treatments resulted in elevated mycotoxin concentration compared with ears without simulated injury; however, the severity of injury within a treatment had little effect. Injury to kernels on the side of the ear resulted in greater DON and ZON concentration than injury to tip kernels, grazing injury applied at physiological maturity, or when no injury was simulated. Greater FBs was measured when tip kernel injury was simulated at the blister stage or when side kernel injury was simulated at milk and dent stages compared with noninjured ears, silk clipping, tip injury at milk and dent stages, or grazing injury at physiological maturity. The current study confirms that the risk of mycotoxin accumulation in the Great Lakes region is greater in the presence of ear-feeding insect pests and may differ depending on the feeding behavior of pest species.

Practical Resistance of Ostrinia nubilalis (Lepidoptera: Crambidae) to Cry1F Bacillus thuringiensis maize discovered in Nova Scotia, Canada.

Transgenic maize, Zea mays L., modified to express insecticidal proteins from the bacterium Bacillus thuringiensis Berliner, was introduced in 1996 to control Ostrinia nubilalis Hübner (Lepidoptera: Crambidae), a key maize pest in North America. The high-dose/refuge concept, developed to delay or prevent resistance evolution to this technology, has been exemplified by O. nubilalis as no cases of practical resistance were identified in >20 years. This study documents the first case of practical resistance to Cry1F Bt maize by O. nubilalis in North America. Four collections of O. nubilalis were made from Cry1F maize in Nova Scotia, Canada with unexpected injury (UXI) ranging from 30-70%. Greater survival of UXI collections was observed when larvae were exposed to the highest concentration of 200 ng Cry1F cm-2 in diet-overlay bioassays compared to susceptible laboratory colonies. Larvae also fed and survived on Cry1F leaf tissue in 7 d bioassays. A collection from non-Bt maize, 120 km west of the UXI region, also survived 200 ng Cry1F cm-2, but was susceptible to Cry1F leaf tissue. Detection of Cry1F-resistant O. nubilalis in what might be considered an insignificant maize-growing region indicates that a number of preventable causal factors may have been related to inadequate stewardship of Bt maize technology.

Susceptibility of Different Instars of Striacosta albicosta (Lepidoptera: Noctuidae) to Vip3A, a Bacillus thuringiensis (Bacillaceae: Bacillales) Protein.

Striacosta albicosta (Smith) (Lepidoptera: Noctuidae) is an important pest of corn, Zea mays L. in the Great Lakes region, which can be controlled by transgenic corn expressing Vip3A protein from Bacillus thuringiensis. To inform insect resistance management, the susceptibility, survival, and development of first, third, and fifth instar S. albicosta to Vip3A was determined using protein-overlay and corn tissue bioassays. Tissue bioassays were also used to determine the quantity of corn tissues with and without Vip3A-expression consumed by various instars. In diet bioassays, third and fifth instars were significantly less susceptible to Vip3A compared with first instars; however, no significant difference was observed in susceptibility of older instars. In tissue bioassays, survival was lowest for larvae fed Vip3A-expressing tissues, ranging from 0 to 21%, however, developmental measures of larvae fed Vip3A-expressing tissues did not differ from those fed artificial diet or tissues of other Bt events. Consumption of Vip3A × Cry1Ab tissues did not differ from that of Cry1Ab for each instar. Estimated Vip3A exposure of first instars ranged from 3 to 57 times higher than the concentration required for 99% mortality (LC99) based on the product of the reported Vip3A expression in transgenic corn tissues and the consumption observed in tissue bioassays; however, the estimated exposure of third and fifth instars to Vip3A was lower than their respective LC99. These findings suggest that first instar S. albicosta maybe exposed to a high dose of Vip3A under field conditions; however, Vip3A-expression in corn may not be high dose against older instars, increasing the risk of resistance development.

Neonicotinoid insecticide residues in subsurface drainage and open ditch water around maize fields in southwestern Ontario.

Neonicotinoids are widely used class of insecticides. Most are seed treatments and during planting active ingredient may be abraded and lost in fugitive dust. Much of this active ingredient contaminates surface waters, exposing aquatic organism to potential ill effects. This study examines concentrations of neonicotinoids appearing in tile drains and open ditches around commercial maize fields around planting time where neonicotinoid seed treatments had been used. This sample set represents surface water leaving the point of origin, for which data are sparse. Clothianidin was found more often than thiamethoxam and at higher concentrations; at a median concentration of 0.35 ng/mL in tile drain water and almost twice that (0.68 ng/mL) in ditches into which the tiles are draining after applications of 19 g/ha on seed. This concentration reveals a 40 to 50 fold dilution for neonicotinoid residues between the points where they leave the field in which they were applied and when they are found in nearby streams in a similar ecosystem. Our data support that for a no-observed-effect concentration of 0.3 ng/mL for thiamethoxam there would be between a 1.6 and 100-fold margin of safety to mayflies in most streams if fugitive dust on pneumatic planters were properly mitigated.

Increased expression of a cGMP-dependent protein kinase in rotation-adapted western corn rootworm (Diabrotica virgifera virgifera L.).

A new 'variant' behavior in western corn rootworm (WCR) has resulted in egg-laying into non-cornfields, compared to 'normal' deposition of eggs in cornfields, allowing these insects to circumvent crop rotation. No morphological or genetic characteristics have been defined to differentiate between the normal and variant biotypes. Cyclic GMP-dependent protein kinases (PKG) have been implicated in the regulation of behaviors in vertebrates, insects, and nematodes, including foraging behavior in Drosophila. A cDNA with homology to the Drosophila melanogaster foraging gene (called Dvfor1) was cloned from WCR. The deduced DvFOR1 protein is approximately 70% similar to FOR proteins in Drosophila, silkworm (Bombyx mori) and honeybee (Apis mellifera). It contains a coiled-coil region, two tandem cyclic nucleotide-binding domains, a serine/threonine kinase catalytic domain, and a serine/threonine kinase catalytic domain extension, which are all characteristically found in PKG proteins. Real-time PCR assays of foraging transcript levels in heads of normal and rotation adapted females of WCR obtained from lab-reared insect colonies indicated that the variants had higher levels (25%) of PKG expression than normals. The magnitude of this increase is similar to that observed in Drosophila rover phenotypes compared to sitter phenotypes. However, Diabrotica contains at least two different foraging gene transcripts, which complicates establishing a direct link between the level of gene expression and insect behavior.

Effect of Prothioconazole Application Timing on Fusarium Mycotoxin Content in Maize Grain.

In 2010 and 2011, studies to determine the optimal timing of prothioconazole application (200 g a.i./ha) for reducing Fusarium mycotoxin accumulation in grain were conducted in controlled replicated experiments under small-plot mist-irrigated experiments and in field-scale experiments using two hybrids susceptible to F. gramineaerum infection. A significant decrease in total deoxynivalenol (DON) [DON + 15-acetyl-DON + DON 3-glucoside + 3-acetyl-DON] and zearalenone concentrations was observed when fungicide was sprayed at VT (tasseling) and R1 (silking; P < 0.01) followed by applications at V18 (18th leaf) and R2 (blister; P < 0.05) stages, corresponding to silk completely emerged and fully elongated and to silk emergence and browning, respectively. No reduction in Fusarium graminearum toxins was found after silk senescence (R3 or milk) stage. Moniliformin, fumonisins, beauvericin, enniatins, HT-2 and T-2 toxins were also found in small quantities, and no reduction was observed after treatment ( P > 0.05). Mean reduction (±s.d.) of 59 ± 20% and 57 ± 38% of total DON and zearalenone was observed at full silk elongation, respectively.

The role of field dust in pesticide drift when pesticide-treated maize seeds are planted with vacuum-type planters.

BACKGROUND: Neonicotinoid-contaminated dust escaping pneumatic seeders causes exposure to non-target organisms such as pollinators. Two sources of dust have been reported: abrasion by talc which is added as seed lubricant during planting, and seed-to-seed abrasion occurring during seed handling, distribution and planting. We report a third important source that warrants remediation. Here, soil dust stirred up by planters was found to enter the vacuum air intake near seed metering devices. RESULTS: The mean quantity of dust collected from the exhaust of a commercial pneumatic planter over a number of field sites and situations was 46 g ha-1 , ranging from 5.8 to 184.2 g ha-1 . While the clothianidin concentration in exhaust dust declined with increasing quantity of dust, total clothianidin recovered increased linearly within the study parameters. Up to 2.4 g ha-1 of clothianidin was recovered from planter exhaust, representing approximately 12.6% of the active ingredient applied to seed. A similar pattern occurred in the laboratory on a single standing planter unit using diatomaceous earth as surrogate field dust. CONCLUSION: Field dust in pneumatic metering systems contributes significantly to clothianidin contamination in planter exhaust by seed abrasion. Adding diatomaceous earth as surrogate field dust to the Heubach seed dust protocol accounted for field dust abrasion and distinguished anti-abrasive properties of seed treatments. © 2017 Society of Chemical Industry.

Baseline Susceptibility of Striacosta albicosta (Lepidoptera: Noctuidae) in Ontario, Canada to Vip3A Bacillus thuringiensis Protein.

Striacosta albicosta (Smith; Lepidoptera: Noctuidae) is a pest of corn (Zea mays L.), which has recently expanded its range into Ontario, Canada. Genetically modified corn expressing Vip3A insecticidal protein from Bacillus thuringiensis is a biotechnological option for the control of S. albicosta. To support an insect resistance management program, we conducted a study of baseline susceptibility of 10-field collected S. albicosta populations in Ontario, Canada to Vip3A before widespread commercial adoption. Neonates were exposed to artificial diet overlaid with Vip3A. The LC50 ranged from 22.7 to 53.5 ng Vip3A cm-2. The EC50 ranged from 11.4 to 30.2 ng Vip3A cm-2. There was low inter-population variation in susceptibility to Vip3A, which we believe represents the natural geographical variation in response and not variation caused by previous exposure to selection pressure of the Vip3A protein.

Fusarium graminearum Mycotoxins in Maize Associated With Striacosta albicosta (Lepidoptera: Noctuidae) Injury.

Western bean cutworm, Striacosta albicosta (Smith; Lepidoptera: Noctuidae) has become a key pest of maize, Zea mays (L.), in Ontario, Canada which is challenging to control due to its lack of susceptibility to most Bt-maize events. Injury by S. albicosta may exacerbate Fusarium graminearum (Schwabe; Hypocreales: Nectriaceae) infection through provision of entry points on the ear. The objectives of this study were to: investigate the relationship between injury by S. albicosta and deoxynivalenol (DON) accumulation; evaluate non-Bt and Bt-maize hybrids, with and without insecticide and fungicide application; and determine optimal insecticide-fungicide application timing for reducing S. albicosta injury and DON accumulation. The incidence of injury by S. albicosta and ear rot severity were found to increase DON concentrations under favorable environmental conditions for F. graminearum infection. Incidence of S. albicosta injury was more important than severity of injury for DON accumulation which may be due to larval consumption of infected kernels. The Vip3A × Cry1Ab event provided superior protection from the incidence and severity of S. albicosta injury compared to non-Bt or Cry1F hybrids. Insecticide application to a Vip3A × Cry1Ab hybrid did not reduce injury further; however, lower severity of injury was observed for non-Bt and Cry1F hybrids when pyrethroids or diamides were applied at early VT or R1 stages. DON concentrations were reduced with application of prothioconazole fungicide tank-mixed with insecticide at late VT (before silk browning) or when insecticide was applied at early VT followed by prothioconazole at R1. The application of an insecticide/fungicide tank-mix is the most efficient approach for maize hybrids lacking high-dose insecticidal proteins against S. albicosta and F. graminearum tolerance. Results demonstrate that reducing the risk of DON accumulation requires a strategic approach to manage complex associations among S. albicosta, F. graminearum and the environment.

Occurrence of Penicillium verrucosum, ochratoxin A, ochratoxin B and citrinin in on-farm stored winter wheat from the Canadian Great Lakes Region.

The occurrence of P. verrucosum and ochratoxin A (OTA) were surveyed for 3 and 4 years, respectively. A total of 250 samples was collected from an average of 30 farms during the 2011, 2012, 2013 and 2014 winter seasons. Most storage bins surveyed were typically 11 m high round bins made of corrugated, galvanized steel, with flat-bottoms and conical roofs. Samples of clumped grain contained the most P. verrucosum (p<0.05, n = 10) followed by samples taken from the first load (n = 24, mean = 147±87 CFU/g) and last load (n = 17, mean = 101±77 CFU/g). Five grain samples (2.2%) tested positive for OTA, citrinin and OTB at concentrations of 14.7±7.9, 4.9±1.9 and 1.2±0.7 ng/g, with only three samples exceeding 5 ng/g. Grain samples positive for OTA were related to moisture resulting from either condensation or migrating moist warm air in the bin or areas where precipitation including snow entered the bin. Bins containing grain and clumps contaminated with OTA were studied in detail. A number of statistically-significant risk factors for OTA contamination were identified. These included 1) grain clumps accumulated around or directly under manhole openings, 2) debris and residue of old grain or grain clumps collected from the bin walls or left on storage floor and augers and 3) grain clumps accumulated around side doors. Even when grain enters storage below the 14.5% threshold of moisture, condensation and moisture migration occurs in hotspots in modern corrugated steel storage bins. Hot spots of OTA contamination were most often in areas affected by moisture migration due to inadequate aeration and exposure to moisture from precipitation or condensation. Further, we found that the nature of the condensation affects the nature and distribution of small and isolated areas with high incidence of toxin contamination and/or P. verrucosum prevalence in the grain bins examined.

Concentration and movement of neonicotinoids as particulate matter downwind during agricultural practices using air samplers in southwestern Ontario, Canada.

Atmospheric emissions of neonicotinoid seed treatment insecticides as particulate matter in field crops occur mainly for two reasons: 1) due to abraded dust of treated seed generated during planting using vacuum planters, and 2) as a result of disturbances (tillage or wind events) in the surface of parental soils which release wind erodible soil-bound residues. In the present study, concentration and movement of neonicotinoids as particulate matter were quantified under real conditions using passive and active air samplers. Average neonicotinoid concentrations in Total Suspended Particulate (TSP) using passive samplers were 0.48 ng/cm2, trace, trace (LOD 0.80 and 0.04 ng/cm2 for clothianidin and thiamethoxam, respectively), and using active samplers 16.22, 1.91 and 0.61 ng/m3 during planting, tillage and wind events, respectively. There was a difference between events on total neonicotinoid concentration collected in particulate matter using either passive or active sampling. Distance of sampling from the source field during planting of treated seed had an effect on total neonicotinoid air concentration. However, during tillage distance did not present an effect on measured concentrations. Using hypothetical scenarios, values of contact exposure for a honey bee were estimated to be in the range from 1.1% to 36.4% of the reference contact LD50 value of clothianidin of 44 ng/bee.