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1.
Ethn Health ; 29(2): 220-238, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37938146

RESUMO

OBJECTIVES: Depression is a major public health concern due to its high prevalence and association with functioning. Ethnic minorities in Western countries are more likely to experience economic disadvantage and exposure to stressors that may put them at higher risk of developing depression. One major protective factor associated with reduced depressive symptoms is an existing support network. This study examined the associations between economic disadvantage, formal and informal social support, and depressive symptoms among two ethnicity groups in Israel: Arab and Jewish mothers of young children, as well as the potential mediating role of formal and informal social support in the associations between economic disadvantage and depressive symptoms. DESIGN: We recruited a representative sample of 837 Jewish and Arab mothers of children aged 2-6 years. We collected data via structured face-to-face interviews following approval of the university ethic committee. We used the Center for Epidemiologic Studies Depression Scale (CES-D), the Medical Outcomes Study (MOS) scale to measure maternal perceived social support, and a measure designed for this study to quantify formal social support. RESULTS: Compared to the Jewish mothers, Arab mothers reported more depressive symptoms, greater economic disadvantage, and fewer informal and formal support networks. Economic disadvantage was negatively associated with informal support but positively associated with formal support among both Jewish and Arab mothers. Results further revealed that informal and formal social support mediated the associations between economic disadvantage and symptoms of depression. CONCLUSIONS: More attention should be paid to the associations between social determinants and mothers' mental health, with a possible shift of focus to macro-level factors, such as economic inequality and minority status.


Assuntos
Árabes , Depressão , Criança , Feminino , Humanos , Pré-Escolar , Árabes/psicologia , Depressão/psicologia , Israel/epidemiologia , Judeus/psicologia , Apoio Social
2.
Int J Psychol ; 2024 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-38837397

RESUMO

This study focused on the implications of the COVID-19 pandemic on the academic experience of university students and their plans for dropping out. We examined how their health, exposure to COVID, and personal COVID-related health risk were associated with perceived social support and a positive academic experience versus plans to drop out of academic studies. We questioned 10,635 students from six research universities in Israel in a cross-sectional online survey. Our findings confirmed that exposure to COVID-19 was positively associated with perceived social support and plans to drop out of academic studies, but negatively associated with a positive academic experience. The greater the perceived social support, the more positive the academic experience, with fewer plans to drop out academically. The more negative students' perceptions of their general health, the more frequent the plans to drop out of academic studies. Also, perceived social support and a positive academic experience mediated the associations between COVID-19 exposure and health status, and plans to drop out of academic studies. This study highlights the potentially positive role of social support and positive academic experiences as significant resources and potential protective factors against plans brought on by COVID to drop out of academic studies.

3.
J Relig Health ; 2024 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-38530581

RESUMO

According to official data, the ultra-Orthodox group in Israel had the highest COVID-19 infection rate yet the lowest vaccination rate compared to the general population. The present study aimed to explore the rate of vaccine uptake as well as reported reasons for vaccine avoidance. In addition, we examined whether several protection motivation theory (PMT) components are good predictors of vaccine uptake. The components we addressed were: perceived susceptibility to the threat of COVID-19, perceived severity of the virus, and perceived efficiency and safety of the vaccine (i.e., response efficacy). The sample included 623 individuals (337 men) aged 18 + who were drawn from a database of a survey company specializing in the ultra-Orthodox community. We conducted a cross-sectional online survey between June 22, 2021, and July 7, 2021, approximately six months after the beginning of vaccination distribution. Results revealed that 65.8% of the participants (versus 89% of the general population) were vaccinated. Women were vaccinated at lower rates than men, whereas those in the Misnagdim ultra-Orthodox subgroup were vaccinated at higher rates than other subgroups in that community. The most prominent reasons for vaccine avoidance were perceived immunity based on prior infection by the virus and lack of trust in the vaccine's safety. In support of the PMT model, the perceived severity of the virus and the vaccine high efficacy were significant predictors of vaccine uptake. The study results call for better outreach to this community and specific psycho-education interventions tailored for its women.

4.
Fam Process ; 62(4): 1608-1623, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-36572646

RESUMO

This study sought to investigate a positive dimension of coping with the COVID-19 pandemic, that of post-traumatic growth (PTG). This study investigated coping difficulties and PTG amongst parent and nonparent students in Israeli Universities. A total of 4022 parents (3648 Jews and 374 Palestinian-Arab Citizens [PACs]) and 14,651 nonparents (12,010 Jews and 2641 PACs) completed measures of coping, social support and PTG. Parents demonstrated significantly higher levels of coping and PTG. Amongst parents, fathers coped slightly better than mothers; however, while Jewish mothers demonstrated greater PTG than Jewish fathers, PAC fathers had significantly greater PTG than both PAC mothers and Jewish parents. These findings, while specific to COVID-19, indicate that PTG should be studied in greater depth in different ethnic and minority groups in order to develop enhanced understanding and facilitate promotion of post-traumatic growth, in addition to the prevention of COVID-19-related distress.


Assuntos
COVID-19 , Crescimento Psicológico Pós-Traumático , Transtornos de Estresse Pós-Traumáticos , Feminino , Humanos , Universidades , Pandemias/prevenção & controle , Estudantes , Adaptação Psicológica , Transtornos de Estresse Pós-Traumáticos/epidemiologia
5.
J Community Psychol ; 51(7): 3012-3028, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37459279

RESUMO

Using the Conservation of Resources (COR) theory, we aimed to determine whether university students' loss and gain in resources during the pandemic mediated the relationships between COVID-19 exposure/concern and depression and anxiety. In 2021, 7013 Israeli university students completed online questionnaires. We assessed the data using structural equation modeling. We found that resource loss was directly related to concern (ß = 0.32, p < 0.001). Depression and anxiety were both directly related to loss of resources (depression: ß = 0.75, p < 0.001; anxiety: ß = 0.69, p < 0.001) and concern (depression: ß = 0.06, p < 0.001; anxiety: ß = 0.12, p < 0.001). Resource loss had an indirect effect on the relationships between concern and depression (ß = 0.24, p < 0.001) and concern and anxiety (ß = 0.22, p < 0.001). COR theory may help explain the mechanisms behind university students' mental health during the pandemic and provides a framework to better understand preparedness for future pandemics, epidemics, and major disasters. Governments and universities should help prevent the loss of resources during future emergencies.


Assuntos
COVID-19 , Angústia Psicológica , Humanos , Pandemias , Universidades , Estudantes
6.
Int J Psychol ; 58(1): 7-15, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36617992

RESUMO

This study examined differences between Arab and Jewish undergraduate students in their perceived academic challenges, COVID-19-related concerns, perceived social support and trust in the university and the government. It also examined factors associated with perceived academic challenges and the moderating role of trust in the university in the associations between COVID-19-related concerns and perceived academic challenges. The sample consisted of 2751 students: 2291 (83%) Jewish, 398 (15%) Arab and 61 (2%) 'other'. Arab students reported higher COVID-19-related concerns and perceived academic challenges, and lower levels of perceived social support and trust in university and government than did Jewish students. Both Jewish and Arab students identified COVID-19-related concerns as a stress-vulnerability factor for perceived academic challenges, whereas perceived social support and trust in the university were identified as resources for perceived academic challenges. No support was found for the role of trust in government in the face of perceived academic challenges nor for the moderating role of trust in the university in the associations between COVID-19-related concerns and perceived academic challenges. In conclusion, the need of Arab students for emotional and academic support needs to be acknowledged as part of the efforts to promote academic success during the pandemic.


Assuntos
Árabes , COVID-19 , Humanos , Árabes/psicologia , Judeus/psicologia , Estudantes/psicologia , Emoções
7.
Health Promot Int ; 37(2)2022 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-34279594

RESUMO

BACKGROUND: The present study examined personality, situational and organizational predictors of burnout during COVID-19 in a military setting, based on the salutogenic theory of health (Antonovsky, 1987). METHOD: Questionnaires were completed by 116 reserve Israeli Home Front Command medical staff (71% males). Background variables (e.g., gender), personality variables (self-efficacy and sense of coherence - SOC), situational variables (state-anxiety, self-rated health and sense of threat) and organizational variables (satisfaction with military's and government's handling of the COVID-19 crisis) were measured as predictors of burnout. RESULTS: Females had higher levels of state anxiety and burnout compared to males. Females also reported a lower level of satisfaction with the military's handling of the COVID-19 crisis than males. SOC and state anxiety were the only statistically significant predictors of burnout after controlling for sociodemographic variables. The entire model explained 59.4% of the burnout variance. CONCLUSION: In accordance with salutogenic theory, SOC is associated with active adaptation through use of generalized and specific resistance resources to avoid burnout in a stressful milieu. Psychological support, psychoeducation and simulation training are offered to increase manageability in crisis situations. LIMITATIONS: Following a large dropout rate due to being quarantined, the final sample size was much smaller than planned. Also, although previous longitudinal studies have found SOC to be a causal factor in burnout, the present cross-sectional design limits such conclusions.


Assuntos
Esgotamento Profissional , COVID-19 , Militares , Esgotamento Profissional/epidemiologia , COVID-19/epidemiologia , Estudos Transversais , Feminino , Humanos , Israel/epidemiologia , Masculino , Pandemias
8.
Death Stud ; 46(5): 1266-1275, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-32903171

RESUMO

This study examined parental coping with grief and identified protective factors for better coping among parents who suffered the loss of a child during military service in Israel. Coping indicators included complicated grief, functioning in life tasks, succeeding in living meaningful lives, and personal growth. Participants were 164 parents who had lost children 5-16 years previously. We found strong associations between parents' decision to continue life despite traumatic loss and several indicators of coping. Meaning-making was associated with better functioning and greater personal growth. Practitioners should explore with parents the internal struggles about deciding whether to continue in life.


Assuntos
Luto , Militares , Adaptação Psicológica , Criança , Pesar , Humanos , Israel , Pais , Fatores de Proteção
9.
Int J Psychol ; 57(5): 547-558, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35567307

RESUMO

This study focused on the specific challenges of university students in the face of the COVID-19 pandemic and examined similarities and differences in COVID-related concerns and difficulties in functioning in samples of undergraduate students in five countries. A sample of 4306 undergraduate university students (43.8% males, 56.2% females) from Israel, Kosovo, Ukraine, Cyprus and Germany participated in an anonymous online survey during the first wave of the pandemic, between March and June 2020. Study variables included the assessment of the exposure to COVID-19, perceived health status, specific COVID-related concerns and functional difficulties, social support, and the perceived level of coping. Similar concerns about the uncertainty regarding the termination of the health crisis and worry for the health of family members were identified as the most common concerns in the five countries. Challenges in online learning and financial difficulties were rated as the most central difficulties. Both COVID-related concerns and COVID-related difficulties predicted lower levels of perceived coping. Greater social support was associated with better perceived coping. Policymakers should be informed by the accumulating research showing the substantive relationships between academic difficulties and perceived COVID-related distress and coping.


Assuntos
COVID-19 , Pandemias , Adaptação Psicológica , COVID-19/epidemiologia , Feminino , Humanos , Masculino , Estudantes , Universidades
10.
Soc Work Health Care ; 61(4): 243-260, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35575192

RESUMO

This study describes the conditions under which Israeli social workers in hospital settings operated s during the COVID-19 pandemic, and assesses their perceived support (informal and organizational support) and preparedness for the next pandemic. It further assesses correlates for perceived support and associations between perceived support and preparedness. The participants were 163 social workers from four hospitals who completed an on-line survey. The findings revealed that the level of exposure to COVID-19 and fear of contracting COVID-19 were unrelated to perceived informal and organizational support. Age and having children who are minors living at home moderated the relationship between fear of contracting COVID-19 and both types of perceived support. Each type of perceived support was significantly associated with preparedness beyond age, having minors at home, exposure to COVID-19, and fear of contracting COVID-19. Implications for research and practice are discussed.


Assuntos
COVID-19 , Pandemias , COVID-19/epidemiologia , Criança , Hospitais , Humanos , Israel/epidemiologia , Assistentes Sociais
11.
Public Health Nutr ; 24(11): 3205-3209, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33896440

RESUMO

OBJECTIVE: The effects of adverse childhood experiences (ACE) on children and adolescents' health status such as obesity are understudied. The current study addressed the effect of ACE on obesity status during childhood utilising multiple waves of national panel data. DESIGN: Longitudinal survey. SETTING: Data were drawn from three waves of the second cohort of the National Survey of Child and Adolescent Well-Being (NSCAW II). NSCAW II study sampled cases from Child Protective Services investigations that were closed between February 2008 and April 2009 nationwide. We measured ACE cumulatively and as separate events and stratified by gender. PARTICIPANTS: Totally, 3170 youth births to 14 years of age at baseline. RESULTS: A count measure of ACE is indeed associated with greater odds of obesity during childhood. Differential effects for different types of ACE were also found, most notably neglect. For girls, physical and psychological neglect increased odds of obesity. CONCLUSIONS: Findings support evidence for the importance of using both a count measure of ACE as well as separating out single events by gender.


Assuntos
Experiências Adversas da Infância , Maus-Tratos Infantis , Adolescente , Criança , Estudos de Coortes , Família , Feminino , Humanos , Estudos Longitudinais , Obesidade/epidemiologia
12.
J Community Health ; 46(4): 667-675, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33029678

RESUMO

The COVID-19 pandemic has created a sense of threat, and stress that has surged globally at an alarming pace. University students were confronted with new challenges. This study examined university students' functional difficulties and concerns during COVID-19 pandemic in two countries: Israel and Ukraine. Additionally, it examined the similarities and differences in prediction of COVID-related concerns in both countries. Two large samples of university students were drawn from both countries. Results showed that students' main functional difficulties in both countries were: worries about their family health status and their learning assignments. In both countries, COVID-related functional difficulties and stress associated with exposure to the media added a significant amount of the explained variance of COVID-related concerns after controlling for background variables. In conclusion-while the level of exposure and difficulties may differ by country and context, their associations with students' concerns seem robust. Additionally, repeated exposure to media coverage about a community threat can lead to increased anxiety.


Assuntos
COVID-19/psicologia , Pandemias , Estudantes/psicologia , Estudantes/estatística & dados numéricos , COVID-19/epidemiologia , Feminino , Humanos , SARS-CoV-2 , Universidades
13.
J Neurochem ; 152(3): 333-349, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31608978

RESUMO

In humans, variations in the polysialic acid-producing enzyme ST8SIA2 and disturbances in the cortical inhibitory system are associated with neurodevelopmental psychiatric disorders such as schizophrenia and autism. In mice, the ST8SIA2-dependent formation of polysialic acid during embryonic development is crucial for the establishment of interneuron populations of the medial prefrontal cortex. However, the spatial pattern and the neurodevelopmental mechanisms of interneuron changes caused by loss of ST8SIA2 function have not been fully characterized. Here, we use immunohistochemical analysis to demonstrate that densities of parvalbumin-positive interneurons are not only reduced in the medial prefrontal cortex, but also in the adjacent motor and somatosensory cortices of St8sia2-deficient male mice. These reductions, however, were confined to the rostral parts of the analyzed region. Mice with conditional knockout of St8sia2 under the interneuron-specific Lhx6 promoter, but not mice with a deletion under the Emx1 promoter that targets cortical excitatory neurons and glia, largely recapitulated the area-specific changes of parvalbumin-positive interneurons in the anterior cortex of St8sia2-/- mice. Live imaging of interneuron migration in slice cultures of the developing cortex revealed a comparable reduction of directional persistence accompanied by increased branching of leading processes in slice cultures obtained from St8sia2-/- embryos or from embryos with interneuron-specific ablation of St8sia2. Together, the data demonstrate a cell-autonomous impact of ST8SIA2 on cortical interneuron migration and the distribution of parvalbumin-positive interneurons in the anterior cortex. This provides a neurodevelopmental mechanism for how dysregulation of ST8SIA2 may lead to disturbed inhibitory balance as observed in schizophrenia and autism.


Assuntos
Movimento Celular/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Interneurônios/metabolismo , Sialiltransferases/metabolismo , Animais , Interneurônios/citologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout
14.
Harefuah ; 159(5): 326-331, 2020 May.
Artigo em Hebraico | MEDLINE | ID: mdl-32431120

RESUMO

INTRODUCTION: In January 2020, the World Health Organization (WHO) declared an international emergency due to the outbreak of COVID-19. In March 2020 the WHO announced that the epidemic could be characterized as a pandemic. Subsequently, the WHO acknowledged the importance of providing support to the medical teams, realizing the enormous burden and stress facing this group. The aim of the present study is to review the up-to-date professional literature dealing with the psychological impact of a pandemic outbreak on medical staff. The literature existing so-far is scarce. It does, however, provide evidence of the short- and long-term impact of such epidemics on the mental health of the medical teams. Direct exposure to affected patients, the risk of contagion, a subjectively negative perception of one's medical condition and being in isolation or quarantine, all constitute risk factors for mental symptoms such as depression, anxiety and post-trauma. A sense of control, especially regarding the means of protection and controlling infection, altruistic acceptance of the work-related risks, social support, good team-spirit in the medical unit and support from superiors are all protective factors.


Assuntos
Infecções por Coronavirus/epidemiologia , Corpo Clínico , Pandemias , Pneumonia Viral/epidemiologia , Betacoronavirus , COVID-19 , Surtos de Doenças , Humanos , SARS-CoV-2 , Organização Mundial da Saúde
15.
Curr Psychiatry Rep ; 21(8): 71, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31264027

RESUMO

PURPOSE OF REVIEW: Millions of individuals and families live under continual exposure to threat such as protracted socio-political conflict or community violence facing current and future danger. The construct of continuous traumatic stress (CTS) was suggested as a supplement perspective for the understanding of the specific impact of living under both current and realistic future threat. Yet, the unique parameters, utility, and validity of the construct CTS are underexplored. RECENT FINDINGS: Currently, CTS describes both the exposure and the unique clinical conglomerate of distress. In this paper, we first elaborate on the rationale for the construct of CTS. Then, we present evidence on the consequences of exposure to CTS in the Israeli context of continual ongoing and prolonged political violence. Research on CTS is presented across the life cycle with an emphasis on risk and protective factors from a developmental perspective. We conclude with implications for research, assessment, and interventions.


Assuntos
Exposição à Violência/psicologia , Política , Transtornos de Estresse Pós-Traumáticos/psicologia , Humanos , Israel , Estudos Longitudinais , Fatores de Proteção , Fatores de Risco , Transtornos de Estresse Pós-Traumáticos/prevenção & controle , Transtornos de Estresse Pós-Traumáticos/terapia
17.
Curr Psychiatry Rep ; 18(6): 57, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27087347

RESUMO

This paper reviews the impact of exposure to man-made or natural disasters on adolescent substance use. It covers empirical studies published from 2005 to 2015 concerning (a) the scope of the problem, (b) vulnerable groups and risk and protective factors, and (c) evidence-based interventions. The review suggests a strong link between adolescent substance use and exposure to either man-made or natural disaster. Vulnerable groups include adolescents with previous exposure to traumatic events, living in areas that are continually exposed to disasters, and ethnic minorities. Risk and protective factors at the individual, familial, community, and societal levels are described based on the bioecological model of mass trauma. Given that mass trauma is unfortunately a global problem, it is important to establish international interdisciplinary working teams to set gold standards for comparative studies on the etiology for adolescent substance use in the context of disasters.


Assuntos
Desastres , Transtornos Relacionados ao Uso de Substâncias/etiologia , Populações Vulneráveis , Adolescente , Etnicidade , Humanos , Masculino , Grupos Minoritários , Fatores de Risco , Transtornos Relacionados ao Uso de Substâncias/epidemiologia
18.
Soc Work Health Care ; 54(8): 687-707, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26399489

RESUMO

The aims of this pilot study were: (a) to test the feasibility of prolonged exposure (PE) therapy conducted by a social worker staff on female patients in methadone program clinics who were survivors of child sexual abuse or rape and (b) to examine preliminary outcomes of PE on posttraumatic stress disorder (PTSD), depression, and illicit drug use at pre- and posttreatment, and up to 12-month follow-ups. Twelve female methadone patients who were survivors of child sexual abuse or rape diagnosed with PTSD were enrolled in 13-19 weekly individual PE sessions. Assessments were conducted at pre-, mid-, and posttreatment, as well as at 3, 6, and 12-month follow-ups. The treatment outcomes measures included PTSD symptoms, depressive symptoms, and illicit drug use. Ten of the 12 study patients completed treatment. PTSD and depressive symptoms showed significant reduction. No relapse to illicit drug use was detected. These preliminary results suggest that PE may be delivered by methadone social workers with successful outcomes. Further research should test the efficacy of PE among methadone patients in a randomized control trial with standard care as the control condition.


Assuntos
Sobreviventes Adultos de Maus-Tratos Infantis/psicologia , Abuso Sexual na Infância/psicologia , Terapia Implosiva/métodos , Metadona/uso terapêutico , Entorpecentes/uso terapêutico , Tratamento de Substituição de Opiáceos/psicologia , Transtornos de Estresse Pós-Traumáticos/terapia , Adulto , Criança , Depressão/etiologia , Depressão/psicologia , Feminino , Humanos , Projetos Piloto , Escalas de Graduação Psiquiátrica , Transtornos de Estresse Pós-Traumáticos/etiologia , Resultado do Tratamento
19.
Psychol Trauma ; 16(1): 1-9, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36931842

RESUMO

OBJECTIVE: Hundreds of thousands of people around the world have lost their lives to intergroup violence (IV) over the past two decades. The current study tested a multifactor ecological model, including societal (exposure to trauma), social (exposure to community violence, and conflict with parents), and individual (exposure to microaggression) factors for engagement in IV in two samples of Israeli youths, Jewish (N = 306), and Arab (N = 302). METHOD: Participants filled out self-report questionnaires assessing exposure to trauma, community violence, conflict with parents, microaggression, and engagement in IV. RESULTS: The results showed that Arab youths who indicated higher levels of religiosity and reported experiencing more traumatic events, community violence, and microaggression were more likely to perpetrate IV (OR = 1.31, 1.39, 1.5, 2.28, respectively). Jewish youths who reported exposure to more traumatic events, more conflict with parents, and greater exposure to microaggression events were more likely to perpetrate IV (OR = 2.28, 2.70, 3.25, respectively). CONCLUSION: The current study illuminates the similarities (traumatic events and microaggression) and the differential associations of individual, social, and societal factors and IV among Jewish and Arab youths. Microaggression emerged as the strongest predictor for IV in both groups. These findings highlight the need to inform ecological interventions aimed at mitigating IV in a context-sensitive manner. (PsycInfo Database Record (c) 2023 APA, all rights reserved).


Assuntos
Árabes , Judeus , Humanos , Adolescente , Israel , Violência , Inquéritos e Questionários
20.
J Cell Sci ; 124(Pt 19): 3279-91, 2011 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-21940794

RESUMO

The polysialic acid (polySia) modification of the neural cell adhesion molecule NCAM is a key regulator of cell migration. Yet its role in NCAM-dependent or NCAM-independent modulation of motility and cell-matrix adhesion is largely unresolved. Here, we demonstrate that loss of polySia attenuates tumour cell migration and augments the number of focal adhesions in a cell-cell contact- and NCAM-dependent manner. In the presence or absence of polySia, NCAM never colocalised with focal adhesions but was enriched at cell-cell contacts. Focal adhesion of polySia- and NCAM-negative cells was enhanced by incubation with soluble NCAM or by removing polySia from heterotypic contacts with polySia-NCAM-positive cells. Focal adhesion was compromised by the src-family kinase inhibitor PP2, whereas loss of polySia or exposure to NCAM promoted the association of p59(Fyn) with the focal adhesion scaffolding protein paxillin. Unlike other NCAM responses, NCAM-induced focal adhesion was not prevented by inhibiting FGF receptor activity and could be evoked by NCAM fragments comprising immunoglobulin domains three and four but not by the NCAM fibronectin domains alone or by an NCAM-derived peptide known to interact with and activate FGF receptors. Together, these data indicate that polySia regulates cell motility through NCAM-induced but FGF-receptor-independent signalling to focal adhesions.


Assuntos
Antígeno CD56/metabolismo , Adesões Focais/metabolismo , Receptores de Fatores de Crescimento de Fibroblastos/metabolismo , Ácidos Siálicos/fisiologia , Transdução de Sinais , Linhagem Celular Tumoral , Movimento Celular , Ativação Enzimática , Fibronectinas/metabolismo , Glicosídeo Hidrolases/farmacologia , Glicosídeo Hidrolases/fisiologia , Humanos , Imunoprecipitação , Microscopia de Fluorescência , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Paxilina/metabolismo , Faloidina/metabolismo , Ligação Proteica , Isoformas de Proteínas/metabolismo , Proteínas Proto-Oncogênicas c-fyn/metabolismo
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