1.
Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities.
J Microbiol Methods
; 117: 1-3, 2015 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-26159909
RESUMO
A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.