Ibn al-Haytham's ground theory of distance perception.

The 11th-century Arab scholar, Ibn al-Haytham, in his Optics, offers a detailed, rigorous, empirically oriented explanation of distance perception that may be the first essentially modern, scientific theory of distance perception. Based on carefully described experiments, he argues that for distance to be perceived accurately: (1) the distance must lie along a continuous surface such as the ground; (2) the continuous surface must be visible; (3) the magnitudes of distances along the surface must be perceived and calibrated through bodily interaction (walking and reaching) with them; and finally (4) the distance must be moderate. Al-Haytham's work reached Europe early in the 13th century, and his was the dominant theory of distance perception there for about 400 years. It was superseded early in the 17th century by a theory, based on cues such as convergence and accommodation, of distance seen through empty, mathematized space. Around 1950, an explanation of distance perception strikingly like that of al-Haytham was independently developed by J. J. Gibson, who called his theory the "ground theory" of space perception.

J. J. Gibson's "Ground Theory of Space Perception".

J. J. Gibson's ground theory of space perception is contrasted with Descartes' theory, which reduces all of space perception to the perception of distance and angular direction, relative to an abstract viewpoint. Instead, Gibson posits an embodied perceiver, grounded by gravity, in a stable layout of realistically textured, extended surfaces and more delimited objects supported by these surfaces. Gibson's concept of optical contact ties together this spatial layout, locating each surface relative to the others and specifying the position of each object by its location relative to its surface of support. His concept of surface texture-augmented by perspective structures such as the horizon-specifies the scale of objects and extents within this layout. And his concept of geographical slant provides surfaces with environment-centered orientations that remain stable as the perceiver moves around. Contact-specified locations on extended environmental surfaces may be the unattended primitives of the visual world, rather than egocentric or allocentric distances. The perception of such distances may best be understood using Gibson's concept of affordances. Distances may be perceived only as needed, bound through affordances to the particular actions that require them.

Lab-on-a-chip technologies for proteomic analysis from isolated cells.

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.

An investigation of the components used to calculate staff:student ratios in nursing and midwifery education.

This article describes the results of a census questionnaire sent to all colleges of nursing and midwifery and institutions of further and higher education undertaking English National Board for Nursing, Midwifery and Health Visiting (ENB) validated pre- and post-registration nursing and midwifery courses. The questionnaire was designed to identify the range of components, incorporated into formulae, used by the colleges and institutions to calculate staff:student ratios (SSRs), for each type of course. The results indicate that the majority of colleges and institutions use: [formula: see text] The definitions of staff and student within the formulae were either assumed or variously defined. A total of 17 different formulae for calculating SSRs were identified and 10 different formulae for calculating whole time equivalents. Each of these formulae are given in the article. The advantages and disadvantages for resourcing nurse education arising from the lack of definition surrounding the standardisation of formulae and the components within formulae are discussed. The discussion reflects both the responses given in the open-ended questions in the questionnaire and the debates surrounding the use of SSRs highlighted in the literature.

Spatial compression produced by a stationary low-vision telescope.

PURPOSE: Geometrical analysis of the monocular information for visual space perception predicts that the magnification produced by a low-vision telescope will compress the depth dimension of space. To test this prediction we measured the compression in depth of perceived shape while looking through a stationary telescope. To control for the other aspects of telescopic viewing, apart from magnification, we also measured perception while looking through a plain tube having the same field of view. METHODS: A 2.75x Keplarian telescope was mounted 40 cm above a tabletop patterned with receding stripes. The 11.6 degrees field of view was centered on a series of rectangular stimulus cards lying flat on the table at a distance of 100 cm. Participants monocularly viewed each card through the telescope, or through a tube having the same field of view, and verbally judged the card's perceived length (in depth) relative to its width (in the frontal plane). RESULTS: Perceptual compression of shape was calculated by dividing the perceived proportion (length/width) by the actual proportion. The telescope and the tube both produced significant perceptual compression, but perception was significantly more compressed through the telescope (0.43) than through the tube (0.52). CONCLUSIONS: The magnification produced by a stationary low-vision telescope can result in a compression of perceived depth. In addition, other aspects of telescopic viewing, such as monocular vision, restricted head movements, and a restricted field of view, can together contribute substantially to such compression. Further research is needed to assess the clinical implications of these results.

Protein phase behavior and crystallization: effect of glycerol.

Glycerol is widely used as an additive to stabilize proteins in aqueous solution. We have studied the effect of up to 40 wt % glycerol on the crystallization of lysozyme from brine. As the glycerol concentration increased, progressively larger amounts of salt were needed to crystallize the protein. Like previous authors, we interpret this as evidence for glycerol changing the interaction between lysozyme molecules. We quantitatively model the interprotein interaction using a Derjaguin-Landau-Verwey-Overbeek potential. We find that the effect of glycerol can be entirely accounted for by the way it modifies the dielectric constant and refractive index of the solvent. Quantifying the interprotein interaction by the second virial coefficient, B(2), we find a universal crystallization boundary for all glycerol concentrations.

Non-equilibrium behavior of sticky colloidal particles: beads, clusters and gels.

To understand the non-equilibrium behavior of colloidal particles with short-range attraction, we studied salt-induced aggregation of lysozyme. Optical microscopy revealed four regimes: bicontinuous texture, 'beads', large aggregates, and transient gelation. The interaction of a metastable liquid-liquid binodal and an ergodic to non-ergodic transition boundary inside the equilibrium crystallization region can explain our findings.

Spatial compression and adaptation with the low vision telescope.

PURPOSE: Geometrical analysis of monocular visual information specifying distance shows that a low vision telescope compresses optically specified distances by a factor about equal to its magnification. Using a group of eight visually healthy adults, we investigated the initial perceptual effect of putting on a 2x Galilean telescope and the adaptation produced by wearing the telescope. METHODS: Viewing was monocular, and the environment was only visible through the telescope. Because the telescope reduced the field of view to 13 degrees , we also tested a different group of eight visually normal adults who wore a simple monocular tube that restricted the field of view to 13 degrees . We measured perceived distance in a corridor using a visually directed open-loop walking task with distances ranging from 4 to 8 m. For both groups, monocular distance perception was measured before putting on the viewing device (baseline), immediately after putting on the viewing device (preadaptation), after wearing the viewing device during a 30-minute period of visual-motor activities (postadaptation), and immediately after taking off the viewing device (aftereffect). RESULTS: Comparing preadaptation with baseline measurements, the viewing devices produced a 15.4% initial compression of perceived distance on average. Comparing aftereffect with baseline measurements, the adaptation period produced a negative aftereffect that was 56.5% of the initial compression, thus showing substantial adaptation. The initial compression and the adaptation were highly significant effects, but neither effect was significantly different for the telescope group and the tube group. CONCLUSION: We conclude that free head movements in a structured environment can largely overcome the optically specified compression of distance produced by the 2x magnification of a low vision telescope, but there remains a significant initial compression of perceived distance that is produced by the restricted field of view. This compression can be substantially reduced by a short period of interaction with the environment.

Distance perception mediated through nested contact relations among surfaces.

In complex natural scenes, objects at different spatial locations can usually be related to each other through nested contact relations among adjoining surfaces. Our research asks how well human observers, under monocular static viewing conditions, are able to utilize this information in distance perception. We present computer-generated naturalistic scenes of a cube resting on a platform, which is in turn resting on the ground. Observers adjust the location of a marker on the ground to equal the perceived distance of the cube. We find that (1) perceived distance of the cube varies appropriately as the perceived location of contact between the platform and the ground varies; (2) variability increases systematically as the relating surfaces move apart; and (3) certain local edge alignments allow precise propagation of distance information. These results demonstrate considerable efficiency in the mediation of distance perception through nested contact relations among surfaces.

Distance perception across spatial discontinuities.

We investigated the use of nested contact relations in perceiving the relative distance of locations on discontinuous surfaces. Observers viewed computer-generated displays under monocular static conditions and adjusted a marker to match the perceived distance of a cube. The marker and cube were raised above the ground by two different platforms separated by a gap. The relative heights and distances of the platforms were varied. We found the following: (1) When spatially discontinuous surfaces are coplanar, locations of objects resting on these surfaces appear to be compared directly, bypassing relations with the underlying ground plane. (2) Spatial displacement between the platforms produces a bias, in the direction of the displacement, in the perceived relative locations of objects resting on the platforms. This suggests that local spatial relations between objects and their platforms are only partially integrated with more global spatial relations between the discontinuous surfaces of the platforms.

Utilization of chopped and long alfalfa by dairy heifers.

Two physical forms of alfalfa hay, chopped and long, were fed at maintenance and for ad libitum consumption to dairy heifers to determine effects upon digestibility and energy utilization. Ad libitum intake was associated with lower digestibility of dry matter (56 versus 58%), neutral detergent fiber (45 versus 58%), and acid detergent fiber (45 versus 52%). Energy balance was 1.77 Mcal/day at ad libitum versus .08 Mcal/day at maintenance. Dry matter intake was similar for chopped (5.4 kg/day) versus long alfalfa (5.5 kg/day). Digestibilities of dry matter, neutral detergent fiber, acid detergent fiber, and cellulose were 7 to 10 percentage units lower when heifers were fed the chopped alfalfa. Metabolizable energy intake generally reflected digestible energy intake, which was about 2 Mcal/day less for heifers consuming chopped alfalfa. Heat production was similar for the two physical forms. Consequently, heifers consuming chopped alfalfa had lower energy balance. Reducing particle size of the alfalfa resulted in lower dry matter and energy digestibility, presumably because of increased rate of passage. Increased intake to compensate for depressed energy utilization of the chopped alfalfa did not occur; effects upon animal gain could not be assessed.

Body protein losses estimated by nitrogen balance and potassium-40 counting.

Body protein losses estimated from N balance were compared with those estimated by 40K counting. Six nonlactating dairy cows were fed an adequate N diet for 7 wk, a low N diet for 9 wk, and a replete N diet for 3 wk. The low N diet contained high cell wall grass hay plus ground corn, starch, and molasses. Soybean meal was added to the low N diet to increase N in the adequate N and replete N diets. Intake was measured daily. Digestibilities, N balance, and body composition (estimated by 40K counting) were determined during each dietary regimen. During low N treatment, hay dry matter intake declined 2 kg/d, and supplement increased about .5 kg/d. Dry matter digestibility was not altered by N treatment. Protein and acid detergent fiber digestibilities decreased from 40 and 36% during adequate N to 20 and 2%, respectively, during low N. Fecal and urinary N also declined when cows were fed the low N diet. By the end of repletion, total intake, fiber, and protein digestibilities as well as N partition were similar to or exceeded those during adequate N intake. Body protein (N) loss was estimated by N balance to be about 3 kg compared with 8 kg by 40K counting. Body fat losses (32 kg) were large because of low energy digestibility and intake. Seven kilograms of body fat were regained during repletion, but there was no change in body protein.