Probe beam deflection optical imaging of thermal and mechanical phenomena resulting from nanosecond electric pulse (nsEP) exposure in-vitro.

Electric-field induced physical phenomena, such as thermal, mechanical and electrochemical dynamics, may be the driving mechanism behind bioeffects observed in mammalian cells during exposure to nanosecond-duration electric pulses (nsEP) in-vitro. Correlating a driving mechanism to a biological response requires the experimental measurement and quantification of all physical dynamics resulting from the nsEP stimulus. A passive and electromagnetic interference (EMI) immune sensor is required to resolve these dynamics in high strength electric fields. The probe beam deflection technique (PBDT) is a passive and EMI immune optical method for quantifying and imaging refractive index gradients in liquids and gases, both dynamic and static, with nanosecond temporal resolution. In this work, a probe beam deflection imaging system was designed to acquire 2-D time-lapse images of thermal/mechanical dynamics resulting from monopolar and bipolar nsEP stimulus.

Probe beam deflection technique as acoustic emission directionality sensor with photoacoustic emission source.

The goal of this paper is to demonstrate the unique capability of measuring the vector or angular information of propagating acoustic waves using an optical sensor. Acoustic waves were generated using photoacoustic interaction and detected by the probe beam deflection technique. Experiments and simulations were performed to study the interaction of acoustic emissions with an optical sensor in a coupling medium. The simulated results predict the probe beam and wavefront interaction and produced simulated signals that are verified by experiment.

Fiber Bragg Grating sensor for fault detection in radial and network transmission lines.

In this paper, a fiber optic based sensor capable of fault detection in both radial and network overhead transmission power line systems is investigated. Bragg wavelength shift is used to measure the fault current and detect fault in power systems. Magnetic fields generated by currents in the overhead transmission lines cause a strain in magnetostrictive material which is then detected by Fiber Bragg Grating (FBG). The Fiber Bragg interrogator senses the reflected FBG signals, and the Bragg wavelength shift is calculated and the signals are processed. A broadband light source in the control room scans the shift in the reflected signal. Any surge in the magnetic field relates to an increased fault current at a certain location. Also, fault location can be precisely defined with an artificial neural network (ANN) algorithm. This algorithm can be easily coordinated with other protective devices. It is shown that the faults in the overhead transmission line cause a detectable wavelength shift on the reflected signal of FBG and can be used to detect and classify different kind of faults. The proposed method has been extensively tested by simulation and results confirm that the proposed scheme is able to detect different kinds of fault in both radial and network system.

Characterization of Pressure Transients Generated by Nanosecond Electrical Pulse (nsEP) Exposure.

The mechanism(s) responsible for the breakdown (nanoporation) of cell plasma membranes after nanosecond pulse (nsEP) exposure remains poorly understood. Current theories focus exclusively on the electrical field, citing electrostriction, water dipole alignment and/or electrodeformation as the primary mechanisms for pore formation. However, the delivery of a high-voltage nsEP to cells by tungsten electrodes creates a multitude of biophysical phenomena, including electrohydraulic cavitation, electrochemical interactions, thermoelastic expansion, and others. To date, very limited research has investigated non-electric phenomena occurring during nsEP exposures and their potential effect on cell nanoporation. Of primary interest is the production of acoustic shock waves during nsEP exposure, as it is known that acoustic shock waves can cause membrane poration (sonoporation). Based on these observations, our group characterized the acoustic pressure transients generated by nsEP and determined if such transients played any role in nanoporation. In this paper, we show that nsEP exposures, equivalent to those used in cellular studies, are capable of generating high-frequency (2.5 MHz), high-intensity (>13 kPa) pressure transients. Using confocal microscopy to measure cell uptake of YO-PRO®-1 (indicator of nanoporation of the plasma membrane) and changing the electrode geometry, we determined that acoustic waves alone are not responsible for poration of the membrane.