Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane.

Pollution induces pressure to soil microorganism; and conversely, the degradation of pollutants is reported largely regulated by the soil microbiome assembly in situ. However, the specific-dependent core taxa of degraders were barely confirmed, which is not conducive to improving the soil remediation strategy. Taking pollution of a typical organochlorine pesticide (OCP), lindane, as an example, we explored the microbial community assembly in flooded soils and simultaneously quantified the corresponding dynamics of typical soil redox processes. Contrasting initial status of microbial diversity was set up by gamma irradiation or not, with additives (acetate, NaNO3, acetate + NaNO3) capable of modifying microbial growth employed simultaneously. Microorganism under lindane stress was reflected by microbial adaptability within complex co-occurrence networks, wherein some environment-dependent core taxa (e.g., Clostridia, Bacteroidia, Bacilli) were highly resilient to pollution and sterilization disturbances. Lindane had higher degradation rate in irradiated soil (0.96 mg kg-1 d-1) than non-irradiated soil (0.83 mg kg-1 d-1). In non-irradiated soil, addition of acetate promoted lindane degradation and methanogenesis, whereas nitrate inhibited lindane degradation but promoted denitrification. No significant differences in lindane degradation were observed in irradiated soils, which exhibited low-diversity microbiomes in parallel to stronger Fe reduction and methanogenesis. The varied corresponding trigger effects on soil redox processes are likely due to differences of soil microbiome, specifically, deterministic or stochastic assembly, in response to pollution stress under high or low initial microbial diversity conditions. Our results improve the knowledge of the adaptability of disturbed microbiomes and their feedback on microbial functional development in OCP-polluted soils, achieving for a more reliable understanding with respect to the ecological risk of soils resided with OCPs under the fact of global microbial diversity loss.

Methane-associated micro-ecological processes crucially improve the self-purification of lindane-polluted paddy soil.

Reductive dechlorination, an efficient pathway for complete removal of organic chlorinated pollutants (OCPs), is commonly reported to be coupled to oxidation of methane (CH4) or methanogenesis in anaerobic environments. However, the relationship between dechlorination and CH4-associated bioprocesses is unclear. Based on the hypothesis that CH4 supplementation could facilitate OCP dechlorination, we investigated the role of CH4-associated bioprocesses in the self-purification of flooded lindane-spiked paddy soils. Four treatments were conducted for up to 28 days: sterilized soil (S), sterilized soil + CH4 (SC), non-sterilized soil (NS), and non-sterilized soil + CH4 (NSC). Results indicated that both sterilization and addition of CH4 promoted lindane degradation and CH4 emissions in the flooded paddy soils. In the NS treatment, lindane had the lowest degradation rate when CH4 emissions were barely detected; while in the SC treatment, lindane had the highest degradation rate when CH4 achieved its highest emissions from anaerobic soil. Also, sterilization led to microbial diversity loss and functional recession, but increased ferrous ion [Fe(II)] concentrations compared to non-sterilized soils. Methanogenic communities and mcrA gene recovered faster than the majority of microorganisms (e.g., Fe bacteria, Bdellovibrionaceae, Rhizobiaceae, Dehalogenimonas) or functional genes (e.g., Dhc, Geo, narG, nirS). Collectively, we assume the enhanced removal of lindane may partly be due to both abiotic dechlorination promoted by chemical Fe redox processes and methanogenesis-derived biotic dechlorination. Revealing the coupling between dechlorination and CH4-associated bioprocesses is helpful to resolve both pollution remediation and mitigation of CH4 emissions in anaerobic contaminated sites.

Crop-dependent root-microbe-soil interactions induce contrasting natural attenuation of organochlorine lindane in soils.

Plant-specific root-microbe-soil interactions play an indisputable role in microbial adaptation to environmental stresses. However, the assembly of plant rhizosphere microbiomes and their feedbacks in modification of pollution alleviation under organochlorine stress condition is far less clear. This study examined the response of root-associated bacterial microbiomes to lindane pollution and compared the dissipation of lindane in maize-cultivated dry soils and rice-cultivated flooded soils. Results showed that lindane pollution dramatically altered the microbial structure in the rhizosphere soil of maize but had less influence on the microbial composition in flooded treatments regardless of rice growth, when the reductive dechlorination of lindane was actively coupled with natural redox processes under anaerobic conditions. After 30 days of plant growth, lindane residues dissipated much faster in anaerobic than in aerobic environments, with only 1.08 mg kg-1 lindane remaining in flooded control compared to 12.79 mg kg-1 in dry control soils. Compared to the corresponding unplanted control, maize growth significantly increased, but rice growth slightly decreased the dissipation of lindane. Our study suggests that opposite impacts would lead to the self-purification of polluted soils during the growth of xerophytic maize and hygrocolous rice. This was attributed to the contrasting belowground micro-ecological processes regarding protection of root tissues and thereby assembly of rhizosphere microbiomes shaped by the xerophytic and hygrocolous crops under different water managements, in response to lindane pollution.

Pollution adaptive responses of root-associated microbiomes induced the promoted but different attenuation of soil residual lindane: Differences between maize and soybean.

Microorganisms colonize plant-associated environments and constitute complex communities aided in key functions for nutrient acquisition, disease suppression and abiotic stress resistance. In this study, we evaluated the variation of root-associated microbiomes of two typical farmland crops, maize (Zea mays L.) and soybean (Glycine max L. Merr.) respond to organochlorine pesticide stress, taking lindane as an example. Results showed that there were promoted but different attenuation rates of residual lindane in rhizosphere soils during maize and soybean growth, and the differential is due to the comprehensive effects of plant characters and microbial activities. Organochlorine pollution did not have significant impact on the microbial diversity and populations in all rhizo-compartments, but mostly stimulated the microbial connectivity. The multistep and decreasing processes for root-associated microbiomes of both maize and soybean were spatially different and mainly dependent on the shaping roles of host plants. These results expand our understandings of the organochlorine influence on the underground ecological system in crop-dependent soils.