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1.
Haemophilia ; 23(5): 750-758, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28470853

RESUMO

BACKGROUND: Red blood cells (RBCs) contribute to hemostasis under blood-flow, and anemia might contribute to a hemorrhagic diathesis. The majority of current laboratory techniques to assess hemostasis do not consider the effects of RBCs. An assay to determine the role of RBCs in hemostasis could be beneficial for clinical management. OBJECTIVES: To investigate the influence of RBCs in hemostasis. METHODS: Hemostasis was investigated using a novel microchip flow-chamber system (T-TAS® ) in an anemic patient with von Willebrand disease. Subsequently, the effects of RBCs in total thrombus analysis system (T-TAS) were examined using reconstituted whole blood at various hematocrit levels. RESULTS: In vivo: When the patient was anemic and demonstrated persisted hemorrhagic symptoms despite the maintained adequate von Willebrand factor ristocetin cofactor activity levels, thrombus formation determined by T-TAS was delayed. However, transfusions of RBCs resolved bleeding symptom and, accordingly, the thrombus formation in T-TAS improved. In vitro: Thrombus formation determined by T-TAS at 1000 s-1 was dose-dependent on hematocrit (the time to reach 10 kPa (T10 ): 10.0 ± 0, 9.5 ± 1.4, 6.7 ± 2.4, 2.8 ± 1.6 min at hematocrits of 0%, 12.5%, 25% and 50%, respectively). Markedly defective thrombus formation (T10 >10 min) was confirmed at a hematocrit <25% at 2000 s-1 . CONCLUSION: Red blood cells play an essential role in hemostasis under high shear, and RBC transfusions could be effective for refractory bleeding in patients with anemia. T-TAS measurements appear to reflect the hemostatic consequences of diminished red cell numbers under blood-flow, and could provide a valuable means for monitoring patients.


Assuntos
Anemia/sangue , Anemia/complicações , Eritrócitos/metabolismo , Hemorragia/etiologia , Hemostasia , Resistência ao Cisalhamento , Anemia/diagnóstico , Testes de Coagulação Sanguínea , Pré-Escolar , Eritrócitos/patologia , Feminino , Humanos , Doenças de von Willebrand/sangue , Doenças de von Willebrand/complicações , Doenças de von Willebrand/diagnóstico
2.
J Clin Pharm Ther ; 39(1): 97-101, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24117862

RESUMO

WHAT IS KNOWN AND OBJECTIVES: Belimumab is a recombinant human monoclonal antibody that binds and antagonizes the biological activity of soluble B-lymphocyte stimulator (BLyS) protein. BLyS appears to play a role in the pathogenesis of systemic lupus erythematosus, and the biological profile of belimumab suggests that it may have a therapeutic benefit in the treatment for the disease. In this healthy Japanese subjects study, we investigated the pharmacokinetics and safety of a single subcutaneous and intravenous injection of belimumab administered as a 200 mg/mL liquid formulation. METHODS: This was an open-label, randomized, parallel-group, single-dose study in healthy Japanese subjects. Each subject received a single intravenous infusion or a subcutaneous injection of 200 mg belimumab. The pharmacokinetic parameters and safety parameters including local tolerance (injection site), biomarkers, immunogenicity and adverse events were evaluated up to 70 days post-dosing. RESULTS: After a single intravenous or a subcutaneous administration of 200 mg belimumab, all 16 subjects completed the study. There were no serious adverse events or adverse events related to injection site reactions. All seven adverse events were considered mild or moderate in intensity and deemed unrelated to belimumab except for cellulitis following intravenous administration. The bioavailability of the single subcutaneous dose of 200 mg belimumab in the subjects was estimated to be 77·5%. Time to the maximum serum concentration after subcutaneous injection was 6·5 days (median). The geometric mean terminal half-life was comparable between the two administration routes (17·7 days intravenous and 15·9 days subcutaneous). Serum immunoglobulin G level decreased slightly after each treatment. No subjects were found to produce antibelimumab antibodies. WHAT IS NEW AND CONCLUSIONS: A favourable absolute bioavailability in healthy Japanese subjects was seen following a subcutaneous injection of 200 mg belimumab. Considering the intersubject variability, exposures were consistent with those previously observed in healthy non-Japanese subjects. Safety and biomarker data were also consistent with previous non-Japanese clinical studies.


Assuntos
Anticorpos Monoclonais Humanizados/efeitos adversos , Anticorpos Monoclonais Humanizados/farmacocinética , Adulto , Anticorpos Monoclonais Humanizados/administração & dosagem , Área Sob a Curva , Povo Asiático , Humanos , Imunossupressores/administração & dosagem , Imunossupressores/efeitos adversos , Imunossupressores/farmacocinética , Infusões Intravenosas , Injeções Subcutâneas , Masculino , Pessoa de Meia-Idade , Adulto Jovem
3.
J Clin Pharm Ther ; 38(3): 236-40, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23489210

RESUMO

WHAT IS KNOWN AND OBJECTIVE: Neuraminidase inhibitors are important options for the treatment of infection by the influenza virus. For the treatment of severe influenza, parenteral administration of a neuraminidase inhibitor may be desirable. This study was conducted to evaluate the pharmacokinetic and safety profiles of intravenous zanamivir, an influenza viral neuraminidase inhibitor, in Japanese subjects to further characterize these profiles particularly following relatively high-doses when compared with inhalation doses and to provide reassurance that there are no marked differences with profiles reported for other ethnically different populations. METHODS: Single doses of 100, 300, 600 mg zanamivir were administered to healthy Japanese men in a randomized, double-blind, ascending dose, placebo-controlled, incomplete three-period cross-over study. In period 3, subjects were given 600 mg of zanamivir on day 1, followed by a 60 h washout period and then a 5-day course of 600 mg zanamivir twice daily. Each subjects received two of three active dosages of zanamivir from 100, 300 and 600 mg, and placebo. RESULTS: Adverse events reported in the study were all mild in intensity and resolved without any treatment. The mean AUC0-∞ values after single intravenous administration of 100, 300 and 600 mg were 16768, 53462 and 100400 ng·h/mL, respectively, demonstrating dose proportionality. No accumulation or time variance was observed after 5 days of twice-daily administration of 600 mg zanamivir. Urinary concentrations of zanamivir after single doses ranging from 100 to 600 mg indicated that over 94% of the zanamivir administered was excreted in urine within 24 h. WHAT IS NEW AND CONCLUSION: Single and 5-day BID repeat dosing of 600 mg were safely administered in Japanese healthy subjects. The pharmacokinetic profile of zanamivir after intravenous administration was consistent with previously reported findings in non-Japanese subjects.


Assuntos
Antivirais/efeitos adversos , Antivirais/farmacocinética , Inibidores Enzimáticos/efeitos adversos , Inibidores Enzimáticos/farmacocinética , Zanamivir/efeitos adversos , Zanamivir/farmacocinética , Administração Intravenosa , Adulto , Antivirais/administração & dosagem , Área Sob a Curva , Povo Asiático , Estudos Cross-Over , Relação Dose-Resposta a Droga , Método Duplo-Cego , Inibidores Enzimáticos/administração & dosagem , Humanos , Masculino , Neuraminidase/antagonistas & inibidores , Adulto Jovem , Zanamivir/administração & dosagem
4.
J Dent Res ; 101(6): 686-694, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35001679

RESUMO

Embryonic craniofacial development depends on the coordinated outgrowth and fusion of multiple facial primordia, which are populated with cranial neural crest cells and covered by the facial ectoderm. Any disturbance in these developmental events, their progenitor tissues, or signaling pathways can result in craniofacial deformities such as orofacial clefts, which are among the most common birth defects in humans. In the present study, we show that Rdh10 loss of function leads to a substantial reduction in retinoic acid (RA) signaling in the developing frontonasal process during early embryogenesis, which results in a variety of craniofacial anomalies, including midfacial cleft and ectopic chondrogenic nodules. Elevated apoptosis and perturbed cell proliferation in postmigratory cranial neural crest cells and a substantial reduction in Alx1 and Alx3 transcription in the developing frontonasal process were associated with midfacial cleft in Rdh10-deficient mice. More important, expanded Shh signaling in the ventral forebrain, as well as partial abrogation of midfacial defects in Rdh10 mutants via inhibition of Hh signaling, indicates that misregulation of Shh signaling underlies the pathogenesis of reduced RA signaling-associated midfacial defects. Taken together, these data illustrate the precise spatiotemporal function of Rdh10 and RA signaling during early embryogenesis and their importance in orchestrating molecular and cellular events essential for normal midfacial development.


Assuntos
Fenda Labial , Fissura Palatina , Anormalidades Craniofaciais , Animais , Fenda Labial/genética , Fissura Palatina/genética , Anormalidades Craniofaciais/genética , Desenvolvimento Embrionário , Proteínas Hedgehog/metabolismo , Camundongos , Crista Neural , Tretinoína
7.
Respir Med Case Rep ; 30: 101035, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32190545

RESUMO

Mucormycosis is a life-threatening infectious disease that occurs most commonly in immunocompromised patients such as those with hematological malignancies. Its clinical symptoms and associated radiological findings vary and specific biomarkers and culture characteristics have not been defined. An 85-year-old man who had been treated for myelodysplastic syndrome and tuberculosis for several months presented with subacute fever and worsening left-side chest pain. Contrast-enhanced computed tomography images depicted massive tumor-like consolidation without enhancement, expanding from the left lower lobe. Emboli that did not respond to anticoagulants were detected in the left descending pulmonary artery. Despite intensive treatment he developed multiple organ failure and died 47 days after hospitalization. Gross pathology of a lung autopsy specimen revealed left lower pulmonary arterial emboli and pulmonary infarction, which was concluded to be the direct cause of death. The emboli were histopathologically identified as invasive mycelia in vessels. Mucor sp. was detected via real-time polymerase chain reaction and immunohistopathological analyses revealed that the mold in the blood vessels of lung tissue was partially positive for the mucor antigen. In the present case of Mucor sp. pulmonary emboli in a patient with myelodysplastic syndrome, radiographic findings were hard to distinguish from those typical of a lung abscess.

8.
J Thromb Haemost ; 14(4): 667-74, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27061057

RESUMO

BACKGROUND: The clinical phenotype of von Willebrand disease (VWD) is heterogeneous, and von Willebrand factor ristocetin cofactor activity (VWF:RCo) does not always reflect clinical severity, especially in VWD type 1. We have reported the potential of a microchip flow-chamber system (Total-Thrombus Formation Analysis System [T-TAS®]) for assessing physiologic hemostasis in VWD. Aim To evaluate the relationship between T-TAS, bleeding score (BS) and laboratory test results in type 1 VWD patients. METHODS: Microchips coated with collagen (platelet chip [PL-chip]) or collagen/thromboplastin (AR-chip) were used to assess platelet thrombus formation (PTF) at high shear rates or fibrin-rich PTF at low shear rates, respectively, in whole blood from 50 patients. The times needed for the flow pressure to increase by 10 kPa and 30 kPa (T10 and T30 ) from baseline were calculated from flow pressure curves. BS was determined by the use of a standardized questionnaire. RESULTS: PL-T10 values correlated with BS (R(2) ~ 0.45) better than VWF:RCo (R(2) ~ 0.36), irrespective of the flow rate, whereas AR-T10 showed only a weak correlation with BS (R(2) ~ 0.18). Patients with PL-T10 > 10 min or AR-T10 > 30 min had lower VWF levels and higher BS than those with PL-T10 ≤ 10 min or AR-T10 ≤ 30 min, and the greatest differences were observed with PL-T10. Clinical severity appeared to correlate best with PL-T10 > 8 min. BS was significantly higher in patients with VWF:RCo of < 10 IU dL(-1) than in those with VWF:RCo of 10 IU dL(-1) to < 25 IU dL(-1) and 25-40 IU dL(-1). In patients with VWF:RCo of < 10 IU dL(-1) , BS was significantly higher in those with PL-T10 > 8 min than in those with PL-T10 ≤ 8 min. CONCLUSION: T-TAS could be a useful technique for discriminating and predicting BS in VWD type 1 patients.


Assuntos
Técnicas Analíticas Microfluídicas/instrumentação , Doença de von Willebrand Tipo 1/sangue , Fator de von Willebrand/química , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Criança , Pré-Escolar , Colágeno/química , Feminino , Hemorragia , Hemostasia , Humanos , Lactente , Masculino , Microfluídica , Pessoa de Meia-Idade , Fenótipo , Pressão , Índice de Gravidade de Doença , Resistência ao Cisalhamento , Estresse Mecânico , Inquéritos e Questionários , Tromboplastina/química , Trombose , Adulto Jovem
9.
J Med Chem ; 39(18): 3461-9, 1996 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-8784443

RESUMO

In the course of our search for new antitumor agents in breast cancer, novel amino-substituted flavone derivatives were synthesized and examined for antitumor activities. Among them, 5,4'-diaminoflavone and some of its congeners showed remarkable antiproliferative activity against the estrogen receptor (ER)-positive and estrogen-responsive human breast cancer cell line MCF-7. The activity was observed irrespective of the presence or absence of estrogen. The 5-aminoflavone derivatives (5-AFs) are not classical anti-estrogens because they did not compete with [3H]estradiol to bind the estrogen receptor. Moreover, 5-AFs showed antitumor activity highly selective to the ER-positive breast cancer cell line, and they showed no effects against the ER-negative human cancer cell lines HeLa S3, WiDr, and MDA-MB-453. Although the mechanism of their selective antitumor activity to ER-positive breast cancer cells is unclear, 5-AFs are expected to be a new type of antitumor agents in breast cancer.


Assuntos
Antineoplásicos/síntese química , Neoplasias da Mama/tratamento farmacológico , Flavonoides/síntese química , Antineoplásicos/farmacologia , Estradiol/farmacologia , Feminino , Flavonoides/farmacologia , Células HeLa , Humanos , Receptores de Estrogênio/análise , Relação Estrutura-Atividade , Células Tumorais Cultivadas
10.
J Med Chem ; 40(12): 1894-900, 1997 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-9191967

RESUMO

Recently, we reported that 5,4'-diaminoflavone (1) exhibits potent and specific growth-inhibitory activity against the estrogen receptor (ER)-positive human breast cancer cell line MCF-7. However, when compound 1 was incubated with S-9 mix, its metabolites were observed. Moreover, addition of S-9 mix to the medium caused the drastic decrease in activity of compound 1. Since the 6-, 8-, and 3'-positions were considered to be metabolized oxidatively in vivo from MO calculations, a series of 5,4'-diaminoflavone derivatives substituted at such putative metabolic positions with various functional groups were synthesized aiming at the metabolically stable derivatives. Among them, 5,4'-diamino-6,8,3'-trifluoroflavone (14d) exhibited strong growth-inhibitory activity against MCF-7 cells even in the presence of S-9 mix. Moreover, orally administered compound 14d completely suppressed the growth of MCF-7 inoculated into nude mice, and the effect was more potent than that of compound 1. In addition to ER-positive breast cancer cells, compound 14d exhibited growth-inhibitory activity against a panel of human cancer cell lines including a part of ER-negative breast, endometrial, ovarian, and liver cancers. From these results, fluorine introduction to the putative metabolic positions of compound 1 was elucidated to be effective in the enhancement of the in vivo antitumor activity, probably due to the block of the metabolic deactivation.


Assuntos
Antineoplásicos/síntese química , Desenho de Fármacos , Animais , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Neoplasias da Mama/química , Neoplasias da Mama/patologia , Divisão Celular/efeitos dos fármacos , Neoplasias do Endométrio/patologia , Feminino , Flavonoides/síntese química , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Humanos , Neoplasias Hepáticas/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Estrutura Molecular , Transplante de Neoplasias , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Ovarianas/patologia , Receptores de Estradiol/análise , Relação Estrutura-Atividade , Células Tumorais Cultivadas
11.
J Med Chem ; 37(7): 1028-32, 1994 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-8151613

RESUMO

The 7-substituted 6H-pyrazolo[4,5,1-de]acridin-6-ones with (aminoalkyl)amino and/or (hydroxyalkyl)amino groups in the side chains were synthesized by bromination using N-bromosuccinimide and the subsequent reaction with amines from the 7-substituted 5-bromo-2-methyl-6H-pyrazolo-[4,5,1-de]acridin-6-one. The substitution reaction of the amines with alkyl bromide (the C2 position) and aryl bromide (the C5 position) was accomplished by choosing the proper reaction conditions. These compounds show DNA intercalating ability in ethidium fluorescence assay and antiproliferative activity against Hela S3 cells. Impressive antitumor activity in vivo against murine P388 leukemia and murine sarcoma 180 solid tumor in mice was demonstrated for the 7-hydroxy analogs. In addition, some of these showed excellent antitumor activity against adriamycin-resistant murine P388 leukemia (P388/ADM) in mice.


Assuntos
Aminoacridinas/síntese química , Antineoplásicos/síntese química , Pirazóis/síntese química , Aminoacridinas/farmacologia , Animais , Antineoplásicos/farmacologia , Divisão Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Leucemia P388/tratamento farmacológico , Camundongos , Estrutura Molecular , Pirazóis/farmacologia , Sarcoma 180/tratamento farmacológico , Células Tumorais Cultivadas
12.
J Am Soc Mass Spectrom ; 3(5): 563-74, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24234500

RESUMO

Nanoscale separation techniques, nanoscale packed capillary columns (75 µm id), and capillary zone electrophoresis (CZE), on-line with electrospray mass spectrometry (ESI/MS), were applied to the separation of a series of ten macrolide antibiotics. Both techniques use sub-microliter-per-minute flow rates through the analytical column and therefore require an electrospray probe that incorporates coaxial sheath flow. Positive ion electrospray mass spectra of these compounds yielded mainly protonated molecules. Fragmentation to yield structurally significant fragment ions was achieved by collision-induced dissociation (CID) at increased skimmer voltages. Separations were achieved using both techniques, with CZE/ESI/MS showing improved peak shapes and detection limits combined with faster analysis times. Nanoscale packed capillary columns provided better chromatographic resolution and was less susceptible to peak broadening caused by overloading of the analytes.

13.
J Biochem ; 99(1): 311-4, 1986 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-3754255

RESUMO

Vibrio sp. isolated from a xanthid crab, Atergatis floridus, was cultured, and tetrodotoxin (TTX) and anhydroTTX were indicated to be present in several fractions of the cell extract and the culture medium by reverse phase HPLC. The presence of the C9-base in alkaline hydrolyzates of these fractions was confirmed by GC-MS and UV spectrometry. These results showed the production of TTX and anhydroTTX in the Vibrio sp., thus indicating one of the origins of TTX in nature.


Assuntos
Braquiúros/microbiologia , Tetrodotoxina/análogos & derivados , Tetrodotoxina/isolamento & purificação , Vibrio/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Meios de Cultura/análise , Concentração de Íons de Hidrogênio , Intestinos/microbiologia , Espectrometria de Massas
14.
Toxicon ; 32(2): 175-83, 1994 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-8153956

RESUMO

Two novel paralytic toxins were isolated from toxic specimens of a xanthid crab Zosimus aeneus inhabiting Ishigaki Island, Okinawa. The structures of two of these were deduced to be carbamoyl-N-hydroxysaxitoxin and carbamoyl-N-hydroxyneosaxitoxin based on electrophoresis, high performance liquid chromatography, electrospray ionization mass spectrometry, 1H NMR, 13C NMR and conversion experiments. They showed specific toxicities of 1700 and 1400 mouse units per mg on i.p. injection into mice.


Assuntos
Braquiúros/química , Saxitoxina/análogos & derivados , Animais , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Camundongos , Oxirredução , Saxitoxina/análise
15.
Toxicon ; 39(2-3): 415-8, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-10978763

RESUMO

Instead of tetrodotoxin, significant amounts of tetrodotoxin-related substances with no mouse lethality were detected in the nontoxic liver specimen of puffer fish, Takifugu xanthopterus. The tetrodotoxin-related substances, which were demonstrated to be tetrodotoxin derivatives by gas chromatography-mass spectrometry analysis, were similar to tetrodonic acid in HPLC but distinguishable from it in electrophoresis. Our results suggest that nontoxic puffer fish contains nontoxic tetrodotoxin derivatives as precursors or metabolites of tetrodotoxin.


Assuntos
Peixes Venenosos , Fígado/química , Tetrodotoxina/isolamento & purificação , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Gasosa-Espectrometria de Massas , Camundongos , Tetrodotoxina/toxicidade
16.
Toxicon ; 38(6): 763-73, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10695964

RESUMO

In 1998, during the surveillance of the toxicity of various marine fouling organisms in Hiroshima Bay, Hiroshima Prefecture, Japan, specimens of the ribbon worm, "himomushi" Cephalothrix sp. (Nemertean) adherent to the shells of cultured oysters hanging onto floating culture rafts were found to contain toxins which showed strong paralytic action in mice throughout the survey period, February to May. The maximum toxicity (as tetrodotoxin, TTX) was 14,734 MU/g whole body. Attempts were made to identify the paralytic toxins in this worm. The "himomushi" toxin (HMT) was extracted from the worm with 80% methanol acidified with acetic acid and the extract defatted with dichloromethane. The aqueous layer was chromatographed on activated charcoal and the unbound and bound toxic fractions were analyzed by high-performance liquid chromatography and gas chromatography-mass spectrometry. It was rather unexpectedly revealed from these results that HMT was comprised of TTX, 4-epiTTX, anhydroTTX and three unidentified toxins. To our knowledge, this is the first report of the occurrence of toxic organisms, containing a high concentration of TTX, adherent to cultured bivalves such as oysters.


Assuntos
Anelídeos/metabolismo , Toxinas Marinhas/análise , Toxinas Marinhas/toxicidade , Ostreidae , Animais , Anelídeos/química , Cromatografia Gasosa-Espectrometria de Massas , Japão , Masculino , Toxinas Marinhas/isolamento & purificação , Camundongos , Paralisia/induzido quimicamente , Simbiose , Tetrodotoxina/análogos & derivados , Tetrodotoxina/análise , Tetrodotoxina/toxicidade
17.
Toxicon ; 33(12): 1577-84, 1995 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8866615

RESUMO

A novel paralytic toxin was isolated from toxic specimens of a xanthid crab Atergatis floridus inhabiting the Pacific coast of Shikoku Island. Its structure was deduced to be 11-saxitoxinethanoic acid (SEA) based on high-performance liquid chromatography, electrospray ionization mass spectrometry, and 1H and 13C-NMR spectrometries. This acid was assumed to exist as an equilibrium mixture of three tautomers, the main tautomer being the hydrate form 11 beta-epimer. SEA showed a specific toxicity of 830 mouse units per mumole on i.p. injection into mice.


Assuntos
Venenos de Peixe/metabolismo , Saxitoxina/isolamento & purificação , Ácido Acético/metabolismo , Animais , Braquiúros , Cromatografia Líquida de Alta Pressão , Venenos de Peixe/química , Venenos de Peixe/isolamento & purificação , Injeções Intraperitoneais , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Camundongos , Padrões de Referência , Saxitoxina/química , Saxitoxina/metabolismo
18.
Toxicon ; 36(4): 627-30, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9643475

RESUMO

A new component of paralytic shellfish poison was isolated from a Bangladeshi freshwater puffer Tetraodon cutcutia. Its structure was deduced to be carbamoyl-N-methylsaxitoxin based on electrospray ionization mass spectrometry, [1H] NMR, and conversion experiments.


Assuntos
Peixes Venenosos , Toxinas Marinhas/química , Saxitoxina/análogos & derivados , Animais , Água Doce , Espectroscopia de Ressonância Magnética , Saxitoxina/química
19.
Toxicon ; 22(3): 425-32, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6474493

RESUMO

Toxins were extracted from the xanthid crab Atergatis floridus inhabiting Ishigaki Island, Okinawa, and subjected to several types of chromatography, resulting in separation into gonyautoxin and saxitoxin fractions. Thin-layer chromatographic and electrophoretic analyses showed that the gonyautoxin fraction was composed of gonyautoxins 1-4, along with some unknown compounds. Gas chromatography-mass spectrometry demonstrated that the gonyautoxin fraction gave rise to the C9-base when alkali-hydrolyzed, indicating that this fraction contained a tetrodotoxin-like compound possessing the quinazoline skeleton specific to tetrodotoxin. The saxitoxin fraction consisted of neosaxitoxin, saxitoxin and two unknown compounds.


Assuntos
Braquiúros/análise , Toxinas Marinhas/análise , Tetrodotoxina/análise , Animais , Cromatografia em Camada Fina/métodos , Eletroforese em Gel de Poliacrilamida/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos
20.
Toxicon ; 26(9): 867-74, 1988.
Artigo em Inglês | MEDLINE | ID: mdl-3201489

RESUMO

Extracts of two species of ribbon worm, Lineus fuscoviridis and Tubulanus punctatus, had lethal potencies in mice from 15-503 MU/g of worm and from less than 10-540 MU/g, respectively. The toxins were partially purified by ultrafiltration and column chromatography using Bio-Gel P-2 and Bio-Rex 70 (H+ form). Thin-layer chromatographic, high performance liquid chromatographic and gas chromatography-mass spectrometric analyses revealed the presence of tetrodotoxin and anhydrotetrodotoxin plus some unidentified compounds.


Assuntos
Toxinas Marinhas/análise , Platelmintos/metabolismo , Tetrodotoxina/análise , Animais , Cromatografia Líquida de Alta Pressão , Masculino , Toxinas Marinhas/toxicidade , Camundongos , Tetrodotoxina/toxicidade
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