Novel measurement system for respiratory aerosols and droplets in indoor environments.

The SARS-CoV-2 pandemic has created a great demand for a better understanding of the spread of viruses in indoor environments. A novel measurement system consisting of one portable aerosol-emitting mannequin (emitter) and a number of portable aerosol-absorbing mannequins (recipients) was developed that can measure the spread of aerosols and droplets that potentially contain infectious viruses. The emission of the virus from a human is simulated by using tracer particles solved in water. The recipients inhale the aerosols and droplets and quantify the level of solved tracer particles in their artificial lungs simultaneously over time. The mobile system can be arranged in a large variety of spreading scenarios in indoor environments and allows for quantification of the infection probability due to airborne virus spreading. This study shows the accuracy of the new measurement system and its ability to compare aerosol reduction measures such as regular ventilation or the use of a room air purifier.

Towards robust data-driven reduced-order modelling for turbulent flows: application to vortex-induced vibrations.

This work presents a robust method that minimises the impact of user-selected parameter on the identification of generic models to study the coherent dynamics in turbulent flows. The objective is to gain insight into the flow dynamics from a data-driven reduced order model (ROM) that is developed from measurement data of the respective flow. For an efficient separation of the coherent dynamics, spectral proper orthogonal decomposition (SPOD) is used, projecting the flow field onto a low-dimensional subspace, so that the dominating dynamics can be represented with a minimal number of modes. A function library is defined using polynomial combinations of the temporal modal coefficients to describe the flow dynamics with a system of nonlinear ordinary differential equations. The most important library functions are identified in a two-stage cross-validation procedure (conservative and restrictive sparsification) and combined in the final model. In the first stage, the process uses a simple approximation of the derivative to match the model with the data. This stage delivers a reduced set of possible library function candidates for the model. In the second, more complex stage, the model of the entire flow is integrated over a short time and compared with the progression of the measured data. This restrictive stage allows a robust identification of nonlinearities and modal interactions in the data and their representation in the model. The method is demonstrated using data from particle image velocimetry (PIV) measurements of a circular cylinder undergoing vortex-induced vibration (VIV) at Re = 4000 . It delivers a reduced order model that reproduces the average dynamics of the flow and reveals the interaction of coexisting flow dynamics by the model structure.

Inflammatory pathways in patients with heart failure and preserved ejection fraction.

UNLABELLED: Immune activation is well established in patients with chronic heart failure and reduced ejection fraction (HF and reduced EF) and is associated with an impaired prognosis. Patients with heart failure and preserved ejection fraction (HF and preserved EF) have an impaired prognosis as well. It is not known whether they have signs of immune activation. METHODS: We studied patients with HF and preserved EF (n=17, NYHA II [n=7]/III [n=10]) and patients with HF and reduced EF (n=17 NYHA II [n=1]/III [n=16]) and 20 controls. Echocardiography demonstrated preserved ejection fraction (LVEF 59+/-9%), but LV hypertrophy in patients with preserved EF as compared with patients with reduced EF (LVEF 23+/-5%). We evaluated levels of TNFalpha, its receptors (sTNFR-1 and 2), IL-6, IL-10 and NT-proBNP. RESULTS: TNFalpha, was highest in HF with reduced EF (2.87+/-0.65 vs 1.67+/-0.58 pg/mL, p<0.001) compared to preserved EF and similar between HF with preserved EF and controls. However, sTNFR1 (1618+/-384 vs 1017+/-302 pg/mL, p<0.001) and sTNFR2 levels (3554+/-916 vs 2041+/-586 pg/mL, p<0.001) in HF with preserved EF were significantly higher compared with controls. The same was true for IL-6, IL-10 and NT-proBNP. The highest cytokine and NT-proBNP levels were present in HF with reduced EF. There was a negative correlation between TNFalpha, and LVEF (r=- 0.700; p<0.0001) and positive correlations between sTNFR1 and 2 with NT-proBNP. CONCLUSION: Patients with HF and preserved EF already show signs of systemic-immune activation which may contribute to the impaired prognosis and the progression to HF with reduced EF.