RESUMO
BACKGROUND: Human pegivirus (HPgV)-formerly known as GBV-C-is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Belém-PA-Brazil. METHODS: Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. RESULTS: The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. CONCLUSIONS: This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon.
Assuntos
Doadores de Sangue , Infecções por Flaviviridae/epidemiologia , Vírus GB C/genética , Pegivirus/genética , RNA Viral/sangue , Viremia/epidemiologia , Adolescente , Adulto , Doadores de Sangue/estatística & dados numéricos , Brasil/epidemiologia , Estudos Transversais , Feminino , Infecções por Flaviviridae/virologia , Vírus GB C/classificação , Vírus GB C/isolamento & purificação , Genoma Viral , Genótipo , Infecções por HIV/complicações , Infecções por HIV/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Pegivirus/classificação , Pegivirus/isolamento & purificação , Filogenia , Prevalência , RNA Viral/genética , Carga Viral , Sequenciamento Completo do Genoma , Adulto JovemRESUMO
We report the genome sequence of Vibrio cholerae strain IEC224, which fails to ferment sucrose. It was isolated from a cholera outbreak in the Amazon. The defective sucrose phenotype was determined to be due to a frameshift mutation, and a molecular marker of the Latin American main epidemic lineage was identified.
Assuntos
Cólera/microbiologia , Epidemias , Genoma Bacteriano , Sacarose/metabolismo , Vibrio cholerae/classificação , Vibrio cholerae/genética , Brasil/epidemiologia , Cólera/epidemiologia , Humanos , Dados de Sequência Molecular , Vibrio cholerae/metabolismoRESUMO
We report the genome sequence of Vibrio cholerae strain IEC224, which fails to ferment sucrose. It was isolated from a cholera outbreak in the Amazon. The defective sucrose phenotype was determined to be due to a frameshift mutation, and a molecular marker of the Latin American main epidemic lineage was identified...(AU)