Patterns of genetic connectedness between modern and medieval Estonian genomes reveal the origins of a major ancestry component of the Finnish population.

The Finnish population is a unique example of a genetic isolate affected by a recent founder event. Previous studies have suggested that the ancestors of Finnic-speaking Finns and Estonians reached the circum-Baltic region by the 1st millennium BC. However, high linguistic similarity points to a more recent split of their languages. To study genetic connectedness between Finns and Estonians directly, we first assessed the efficacy of imputation of low-coverage ancient genomes by sequencing a medieval Estonian genome to high depth (23×) and evaluated the performance of its down-sampled replicas. We find that ancient genomes imputed from >0.1× coverage can be reliably used in principal-component analyses without projection. By searching for long shared allele intervals (LSAIs; similar to identity-by-descent segments) in unphased data for >143,000 present-day Estonians, 99 Finns, and 14 imputed ancient genomes from Estonia, we find unexpectedly high levels of individual connectedness between Estonians and Finns for the last eight centuries in contrast to their clear differentiation by allele frequencies. High levels of sharing of these segments between Estonians and Finns predate the demographic expansion and late settlement process of Finland. One plausible source of this extensive sharing is the 8th-10th centuries AD migration event from North Estonia to Finland that has been proposed to explain uniquely shared linguistic features between the Finnish language and the northern dialect of Estonian and shared Christianity-related loanwords from Slavic. These results suggest that LSAI detection provides a computationally tractable way to detect fine-scale structure in large cohorts.

Stress-associated ß -glucan administration stimulates the TLR - MYD88 - NFKB1 signaling pathway in Nile tilapia (Oreochromis niloticus).

There is evidence that the administration of ß-glucan can effectively activate several defense mechanisms, such as the Tlr-Myd88-Nfkb1 pathway that induces the expression of immune cytokines. Thus, the objective of this work was to evaluate whether ß-glucan acts on the mechanisms of gene transcription via the Tlr-Myd88-Nfkb1 pathway in Nile tilapia under stress after challenge with Streptococcus agalactiae. Therefore, we evaluated the expression of immune system genes such as toll-like receptors 1 (tlr1), toll-like receptors 2 (tlr2), primary myeloid differentiation response gene (myd88) and nuclear factor kappa B1 (nfkb1). A total of 408 fish were distributed in 24 polyethylene boxes and randomly divided into eight groups with 3 replications each: C15: Tilapias received a control diet (free of ß-glucan) for 15 days and were sampled after the 15th day of the experiment; C15D: Tilapias received a control diet (free of ß-glucan) for 15 days, were challenged on the 14th day and were sampled at the 15th day of the experiment; ß15: Tilapias received experimental diet (1g kg-1 of ß-glucan) for 15 days and were sampled after 15 days; ß15D: Tilapias received an experimental diet (1g kg-1 of ß-glucan) for 15 days, were challenged on the 14th day and were sampled at the 15th day of the experiment; C30: Tilapias received a control diet (free of ß-glucan) for 30 days and were sampled on the 30th day of the experiment; C30D: Tilapias received a control diet (free of ß-glucan) for 30 days, were challenged on the 29th day and were sampled at the 30th day of the experiment; ß30: Tilapias received experimental diet (1g kg-1 of ß-glucan) for 30 days and were sampled after 30 days and ß30D: Tilapias received experimental diet (1g kg-1 of ß-glucan) for 30 days, were challenged on the 29th day and were sampled at 30 of the experiment. In the fish sampled at 15 and 30 days of the experiment, after being anesthetized and killed by brain section, cranial kidney and spleen were collected for gene expression analysis. The analyzes showed that the association of ß-glucan and stressful management modulated the immune system, using the Tlr-Myd88-Nfkb1 signaling pathway, indicating that this compound can be used to promote early defense and protect fish against diseases.

Flashing in-curb LEDs and beacons at unsignalized crosswalks and driver's visual attention to pedestrians during nighttime.

Driver's visual attention (eye movements) and driving behaviour (kinematic data) were assessed when approaching an experimental crosswalk that included flashing white in-curb LED strips, flashing orange beacons, backlit 'Yield here to pedestrians' vertical signs, and enhanced lighting when a staged pedestrian attempted to cross. An experimental condition in which all devices were active was compared with a control condition in which only enhanced lighting and backlit vertical signs were active. The results showed a significant increase of motorists' yielding compliance, distance of first fixation to the pedestrian area, standard deviation for horizontal eye movements in the experimental condition. The introduction of flashing in-curb LED strips and flashing orange beacons proved to be very effective in increasing the night-time safety of the pedestrian crossing. Practitioner summary: The study investigated the effects of flashing in-curb LED strips and beacons on driver's visual attention (eye movements) and speed when approaching a crosswalk during night-time. The results showed that the combination of these flashing devices significantly increased yielding compliance and the distance of pedestrian detection. Abbreviations: ANOVA: analysis of variance; EU: European Union; HAWK: high intensity activated crosswalk system; LED: light-emitting diode; PHB: pedestrian hybrid beacons; ROI: region of interest; RRFB: rectangular rapid flashing beacons; UK: United Kingdom; US: United States.

Occurrence of polar organic compounds in atmospheric particulate matter: a system review in South America.

Particulate matter (PM) is one of the existing air pollutants, which can cause damages to human health, public property, and the environment. The chemical composition of this pollutant greatly varies, mainly its organic fraction. Thus, our objective was to carry out a literature review based on articles, considering studies conducted in South America, whose authors address the characterization of the polar organic fraction of PM. We performed the review using the Scopus, SciELO, and Web of Science databases, considering publications dated from the years 2010 to 2019. A total of 14,575 articles were found, of which only 12 met the predefined selection criteria. According to our research, the most studied compound is levoglucosan, a biomass burning marker belonging to the group of anhydrous sugars. Besides, nitro-PAHs, which usually originate from vehicular sources and are compounds with mutagenic and carcinogenic characteristics, have also been found. Moreover, we concluded that, currently, there are few studies on the subject in South America, requiring more research on polar organic compounds present in PM in countries of this region. These studies are of great importance because some compounds can cause great damage to human health, such as the nitro-PAHs; furthermore, PM may still have unknown compounds that need identification and elucidation of their toxicity.

The place of metropolitan France in the European genomic landscape.

Unlike other European countries, the human population genetics and demographic history of Metropolitan France is surprisingly understudied. In this work, we combined newly genotyped samples from various zones in France with publicly available data and applied both allele frequency and haplotype-based methods to describe the internal structure of this country, using genome-wide single nucleotide polymorphism (SNP) array genotypes. We found out that French Basques, already known for their linguistic uniqueness, are genetically distinct from all other groups and that the populations from southwest France (namely the Gascony region) share a large proportion of their ancestry with Basques. Otherwise, the genetic makeup of the French population is relatively homogeneous and mostly related to Southern and Central European groups. However, a fine-grained, haplotype-based analysis revealed that Bretons slightly separated from the rest of the groups, due mostly to gene flow from the British Isles in a time frame that coincides both historically attested Celtic population movements to this area between the 3th and the ninth centuries CE, but also with a more ancient genetic continuity between Brittany and the British Isles related to the shared drift with hunter-gatherer populations. Haplotype-based methods also unveiled subtle internal structures and connections with the surrounding modern populations, particularly in the periphery of the country.

A Bayesian approach for monitoring epidemics in presence of undetected cases.

One of the key indicators used in tracking the evolution of an infectious disease is the reproduction number. This quantity is usually computed using the reported number of cases, but ignoring that many more individuals may be infected (e.g. asymptomatic carriers). We develop a Bayesian procedure to quantify the impact of undetected infectious cases on the determination of the effective reproduction number. Our approach is stochastic, data-driven and not relying on any compartmental model. It is applied to the COVID-19 outbreak in eight different countries and all Italian regions, showing that the effect of undetected cases leads to estimates of the effective reproduction numbers larger than those obtained only with the reported cases by factors ranging from two to ten.

A review of multivariate analysis: is there a relationship between airborne particulate matter and meteorological variables?

Among statistical tools for the study of atmospheric pollutants, trajectory regression analysis (TRA), cluster analysis (CA), and principal component analysis (PCA) can be highlighted. Therefore, this article presents a systematic review of such techniques based on (i) air mass influences on particulate matter (PM) and (ii) the study of the relationship between PM and meteorological variables. This article aims to review studies that use TRA and to review studies that adopt CA and/or PCA to identify the associations and relationship between meteorological variables and atmospheric pollutants. Papers published between 2006 and 2018 and indexed by five of the main scientific databases were considered (ScienceDirect, Web of Science, PubMed, SciELO, and Scopus databases). PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) recommendations supported this systematic review. From the resulting most relevant papers, eight studies analyzed the influence of air mass trajectories on PM using TRA and twenty-one studies searched for the relationship between meteorological variables and PM using CA and/or PCA. A combination of TRA and time series models was identified as the possibility of future works. Besides, studies that simultaneously combine the three techniques to identify both the influence of air masses on PM and its relationship with meteorological variables are a possibility of future papers, because it can lead to a better comprehension of such a phenomenon.

The role of peripheral vision in vertical road sign identification and discrimination.

The role of peripheral vision in road sign identification and discrimination was investigated in two studies. Peripheral vision plays an important role in road signs perception due to their lateral positioning. In the first study 20 participants identified road signs presented at five levels of horizontal eccentricity (1.1°-12.4°), and two levels of vertical eccentricity (0°-2.5°). In the second study road sign discrimination was tested in a same-different discrimination task. The first study showed that a vertical offset of 2.5° degraded proportion correct rate by 9%. Proportion correct rate decreased from 79% to 41% in the transition from 1.1° to 12.4° of horizontal offset. The second study showed an accurate discrimination for road signs presented within a horizontal offset of 6.4°. Road signs with angular shapes and prominent vertexes as triangular or cross signs were better identified in peripheral vision than signs with more compact shapes (circular signs). Practitioner summary: Vertical road signs, due to their lateral positioning, are often perceived in peripheral vision. Horizontal and vertical eccentricity negatively impacts the driver's ability to correctly identify and discriminate traffic signs. The use of singular shapes and a design with simple pictograms and large contrasting areas strongly facilitate road sign perception in peripheral vision.

Geometric Baryogenesis from Shift Symmetry.

We present a new scenario for generating the baryon asymmetry of the Universe that is induced by a Nambu-Goldstone (NG) boson. The shift symmetry naturally controls the operators in the theory while allowing the NG boson to couple to the spacetime geometry as well as to the baryons. The cosmological background thus sources a coherent motion of the NG boson, which leads to baryogenesis. Good candidates of the baryon-generating NG boson are the QCD axion and axionlike fields. In these cases, the axion induces baryogenesis in the early Universe and can also serve as dark matter in the late Universe.

Is cardiovascular disease in patients with diabetes associated with serum levels of MMP-2, LOX, and the elastin degradation products ELM and ELM-2?

BACKGROUND: Diabetes mellitus type 2 (T2DM) is a significant risk factor for the development of cardiovascular diseases (CVDs). In a previous microarray study of internal mammary arteries from patients with and without T2DM, we observed several elastin-related genes with altered mRNA-expression in diabetic patients, namely matrix metalloproteinase 2 (MMP-2), lysyl oxidase (LOX) and elastin itself. In this study we investigate whether the serum concentrations of elastin-related proteins correlate to signs of CVD in patients with T2DM. METHODS: Blood samples from 302 type 2 diabetic patients were analysed for MMP-2, LOX, and the elastin degradation products ELM and ELM2. The results were investigated for correlations to signs of CVD in different vascular territories, as determined by myocardial perfusion scintigraphy, carotid artery thickness and ankle-brachial blood pressure index. RESULTS: T2DM patients with peripheral arterial disease (low ankle-brachial index) (PAD) display higher levels of MMP-2 and ELM compared to patients without PAD. However, none of the proteins or degradation products correlated with myocardial ischemia or a combined measure of CVD-signs, including myocardial ischemia, increased carotid thickness and decreased ankle-brachial blood pressure. CONCLUSIONS: Our results suggest that the diabetic environment affects the circulating amounts of MMP-2 and ELM in patients with PAD. However, the same connection could not be seen in diabetic patients with CVD broadly identified in three vascular territories. LOX and ELM-2 did not correlate to any type of CVD. Overall, serum levels of elastin-related molecules are only remotely related to CVD in type 2 diabetes.

Reflective Tape Applied to Bicycle Frame and Conspicuity Enhancement at Night.

OBJECTIVE: Four studies were conducted to assess bicyclist conspicuity enhancement at night by the application of reflective tape (ECE/ONU 104) to the bicycle rear frame and to pedal cranks. BACKGROUND: Previous studies have tested the benefits of reflective markings applied to bicyclist clothing. Reflective jackets however need to be available and worn while reflective markings enhance conspicuity without any active behavior by the bicyclist. METHOD: In the first study, reflective tape was applied to the rear frame. Detection distance was compared in four conditions: control, rear red reflector, high visibility jacket, and reflective tape. In the second study, the same conditions were studied with night street lighting on and off. In the third study, detection and recognition distances were evaluated in rainy conditions. In the fourth study, visibility was assessed with the reflective tape applied to pedal cranks. RESULTS: In the first study, the application of reflective markings resulted in a detection distance of 168.28 m. In the second study, the detection distance with reflective markings was 229.74 m with public street light on and 256.41 m with public street light off. In rainy conditions, detection distance using the reflective markings was 146.47 m. Reflective tape applied to pedal cracks resulted in a detection distance of 168.60 m. CONCLUSION: Reflective tape applied to the rear bicycle frame can considerably increase bicyclist conspicuity and safety at night. APPLICATION: Reflective tape is highly recommended to complement anterior and rear lights in bicycle riding at night.

PSCF method for source identification of particulate matter in an agricultural background region in Brazil.

The use of mathematical and statistical models to investigate potential sources of pollutants that have been transported by air masses to a study site is important for establishing control and monitoring measures for air pollutants such as PM10 and PM2.5. During the study period, from 2018 to 2021, the concentrations of PM10 and PM2.5 recorded in Ribeirão Preto (SP, Brazil) were higher during spring and winter, with a tendency to increase the amplitude and its maximum values relative to daily averages. The source-receptor model, Potential Source Contribution Function (PSCF), was used to identify probable sources of these pollutants, and the regions known as Triângulo Mineiro and Intermediate Geographic Region of Juiz de Fora (MG, Brazil) were the main regions associated with high PSCF probability values (> 0.5) as sources of PM. These regions indicate that the possible sources of PM emissions are associated with industrial complexes and agriculture, especially coffee production.

Folate-producing bifidobacteria: metabolism, genetics, and relevance.

Folate (the general term for all bioactive forms of vitamin B9) plays a crucial role in the evolutionary highly conserved one-carbon (1C) metabolism, a network including central reactions such as DNA and protein synthesis and methylation of macromolecules. Folate delivers 1C units, such as methyl and formyl, between reactants. Plants, algae, fungi, and many bacteria can naturally produce folate, whereas animals, including humans, must obtain folate from external sources. For humans, folate deficiency is, however, a widespread problem. Bifidobacteria constitute an important component of human and many animal microbiomes, providing various health advantages to the host, such as producing folate. This review focuses on bifidobacteria and folate metabolism and the current knowledge of the distribution of genes needed for complete folate biosynthesis across different bifidobacterial species. Biotechnologies based on folate-trophic probiotics aim to create fermented products enriched with folate or design probiotic supplements that can synthesize folate in the colon, improving overall health. Therefore, bifidobacteria (alone or in association with other microorganisms) may, in the future, contribute to reducing widespread folate deficiencies prevalent among vulnerable human population groups, such as older people, women at child-birth age, and people in low-income countries.

Application of an in vitro new approach methodology to determine relative cancer potency factors of air pollutants based on whole mixtures.

Air pollution is an example of a complex environmental mixture with different biological activities, making risk assessment challenging. Current cancer risk assessment strategies that focus on individual pollutants may overlook interactions among them, potentially underestimating health risks. Therefore, a shift towards the evaluation of whole mixtures is essential for accurate risk assessment. This study presents the application of an in vitro New Approach Methodology (NAM) to estimate relative cancer potency factors of whole mixtures, with a focus on organic pollutants associated with air particulate matter (PM). Using concentration-dependent activation of the DNA damage-signaling protein checkpoint kinase 1 (pChk1) as a readout, we compared two modeling approaches, the Hill equation and the benchmark dose (BMD) method, to derive Mixture Potency Factors (MPFs). MPFs were determined for five PM2.5 samples covering sites with different land uses and our historical pChk1 data for PM10 samples and Standard Reference Materials. Our results showed a concentration-dependent increase in pChk1 by all samples and a higher potency compared to the reference compound benzo[a]pyrene. The MPFs derived from the Hill equation ranged from 128 to 9793, while those from BMD modeling ranged from 70 to 303. Despite the differences in magnitude, a consistency in the relative order of potencies was observed. Notably, PM2.5 samples from sites strongly impacted by biomass burning had the highest MPFs. Although discrepancies were observed between the two modeling approaches for whole mixture samples, relative potency factors for individual PAHs were more consistent. We conclude that differences in the shape of the concentration-response curves and how MPFs are derived explain the observed differences in model agreement for complex mixtures and individual PAHs. This research contributes to the advancement of predictive toxicology and highlights the feasibility of transitioning from assessing individual agents to whole mixture assessment for accurate cancer risk assessment and public health protection.

Percutaneous suprapubic tube bladder drainage after robot-assisted radical prostatectomy: a step-by-step guide.

OBJECTIVE: To describe our technique of maintaining bladder drainage after robot-assisted radical prostatectomy (RARP) using a percutaneous suprapubic tube (PST) in place of a urethral catheter. METHODS: A watertight anastomosis permits placement of the PST. Contraindications include morbid obesity, concomitant inguinal hernia mesh repair, anticoagulation therapy, limited hand dexterity in the patient, bladder neck reconstruction and extensive adhesiolysis at RARP. The necessary equipment includes a 14-F PST balloon catheter set, a three-way connector, a connecting tube, a suture passer, 1/0 polypropylene sutures on a CT1 needle, a sterile plastic button, adhesive and steri-strips. RESULTS: The important steps for PST placement are: Step 1: robot-assisted placement of a bladder wall anchor suture; Step 2: transferring the bladder wall suture to anterior abdominal skin; Step 3: guided placement of the PST under robotic vision; Step 4: securing the PST within the bladder and abdominal wall; Step 5. postoperative care: clamping the PST on postoperative day 5, recording each void and post-void residual urine volumes in a patient diary, removal of the PST on postoperative day 7 after 48 h of voiding with residual urine <100 mL per void. CONCLUSION: We provide a concise step-by-step guide for placement of a PST during RARP as well as important management aspects for the successful adoption of this technique.

Long-term follow-up of patients undergoing percutaneous suprapubic tube drainage after robot-assisted radical prostatectomy (RARP).

UNLABELLED: Study Type - Therapy (case series). Level of Evidence 4 What's known on the subject? and What does the study add? • Initial reports of percutaneous suprapubic tube (PST) drainage following RARP demonstrated feasibility and short-term safety, while decreasing patient discomfort and utilization of anti-cholinergic medication. • This study demonstrates the long-term safety and efficacy of bladder drainage by PST; splinting the urethrovesical anastomosis is simply not essential if mucosal apposition is ensured. OBJECTIVES: • To evaluate the long-term safety and functional outcomes of patients undergoing percutaneous suprapubic tube (PST) drainage after robot-assisted radical prostatectomy (RARP). PATIENTS AND METHODS: • Between January 2008 and October 2009, 339 patients undergoing RARP by one surgeon experienced in RA surgery (M.M.) had postoperative bladder drainage with PST and a minimum of 1-year follow-up for urinary function. • Functional outcomes were obtained via patient-administered questionnaire. • Complications were captured by exhaustive review of multiple datasets, including our prospective prostate cancer database, claims data, as well as electronic medical and institutional morbidity and mortality records. RESULTS: • Urinary function assessed by patient-administered questionnaire was analysed at a mean (sd) follow-up of 11.5 (1.7) months; after RARP with PST placement, 293 patients (86.4%) had total urinary control and only nine (2.7%) required >1 pad/day. • In all, 86 patients (25.4%) never wore a pad; the median time to 0-1 pad/day was 2 weeks (interquartile range [IQR] 0,6); median time to total control was 6 weeks (IQR 1,22). • The mean (sd) follow-up for complications was 23.7 (6.1) months. In all, 15 patients (4.4%) had a procedure-specific complication, of which 13 were minor (Clavien Class I/II 3.8%); one patient had a bladder neck contracture. • In all, 16 patients (4.7%) required Foley placement after RARP for gross haematuria (two patients), urinary retention (three), tube malfunction (four) or need for prolonged Foley catheterization (seven). CONCLUSIONS: • PST placement after RARP is safe and efficacious on long-term follow-up. • Splinting of the urethrovesical anastomosis is not a critical step of RP if a watertight anastomosis and excellent mucosal apposition are achieved.

Laser scanning on road pavements: a new approach for characterizing surface texture.

The surface layer of road pavement has a particular importance in relation to the satisfaction of the primary demands of locomotion, such as security and eco-compatibility. Among those pavement surface characteristics, the "texture" appears to be one of the most interesting with regard to the attainment of skid resistance. Specifications and regulations, providing a wide range of functional indicators, act as guidelines to satisfy the performance requirements. This paper describes an experiment on the use of laser scanner techniques on various types of asphalt for texture characterization. The use of high precision laser scanners, such as the triangulation types, is proposed to expand the analysis of road pavement from the commonly and currently used two-dimensional method to a three-dimensional one, with the aim of extending the range of the most important parameters for these kinds of applications. Laser scanners can be used in an innovative way to obtain information on areal surface layer through a single measurement, with data homogeneity and representativeness. The described experience highlights how the laser scanner is used for both laboratory experiments and tests in situ, with a particular attention paid to factors that could potentially affect the survey.

Fish Liver Discards as a Source of Long-Chain Omega-3 Polyunsaturated Fatty Acids.

The intake of omega-3 polyunsaturated fatty acids (PUFA) of the average consumer is generally low, and products such as fish oils high in omega-3 PUFA have become popular dietary supplements. There is a need for more sources of omega-3 PUFA to cover the increasing demand. This study investigated whether livers from different lean fish species could be a potential new source of oils rich in omega-3 PUFA. The seasonal variation in lipid content, fatty acid composition, peroxide value and free fatty acid content (FFA) of livers from cod, hake, ling, coalfish and monkfish was determined, and the effect of storage conditions on the fishing vessel (ice vs frozen) was studied. Generally, the lipid content and composition of the livers from the five fish species varied similarly during the two years of the sampling period, with significantly lower values in spring (March, April) and higher values in fall (October, November). Storage conditions were found to have no significant effect on the quality and oil composition. Monkfish livers were less suitable for production of omega-3 oil due to their lower lipid and EPA content as well as higher FFA levels. Coalfish had higher fluctuations in oil composition during the sampling period, which potentially makes a standardised quality difficult to obtain. Cod, hake and ling were the most suitable species for fish liver oil production.

Resolution of R-loops by topoisomerase III-ß (TOP3B) in coordination with the DEAD-box helicase DDX5.

The present study demonstrates how TOP3B is involved in resolving R-loops. We observed elevated R-loops in TOP3B knockout cells (TOP3BKO), which are suppressed by TOP3B transfection. R-loop-inducing agents, the topoisomerase I inhibitor camptothecin, and the splicing inhibitor pladienolide-B also induce higher R-loops in TOP3BKO cells. Camptothecin- and pladienolide-B-induced R-loops are concurrent with the induction of TOP3B cleavage complexes (TOP3Bccs). RNA/DNA hybrid IP-western blotting show that TOP3B is physically associated with R-loops. Biochemical assays using recombinant TOP3B and oligonucleotides mimicking R-loops show that TOP3B cleaves the single-stranded DNA displaced by the R-loop RNA-DNA duplex. IP-mass spectrometry and IP-western experiments reveal that TOP3B interacts with the R-loop helicase DDX5 independently of TDRD3. Finally, we demonstrate that DDX5 and TOP3B are epistatic in resolving R-loops in a pathway parallel with senataxin. We propose a decatenation model for R-loop resolution by TOP3B-DDX5 protecting cells from R-loop-induced damage.

Pseudo-Dirac dark matter leaves a trace.

Pseudo-Dirac dark matter is a viable type of dark matter which originates from a new Dirac fermion whose two Weyl states get slightly split in mass by a small Majorana term. The decay of the heavier to the lighter state naturally occurs over a detectable length scale. Thus, whenever pseudo-Dirac dark matter is produced in a collider, it leaves a clear trace: a visible displaced vertex in association with missing energy. Moreover, pseudo-Dirac dark matter behaves Dirac-like for relic abundance and Majorana-like in direct detection experiments. We provide a general effective field theory treatment, specializing to a pseudo-Dirac bino. The dark matter mass and the mass splitting can be extracted from measurements of the decay length and the invariant mass of the products, even in the presence of missing energy.