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1.
Nature ; 599(7886): 622-627, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34759320

RESUMO

Zero hunger and good health could be realized by 2030 through effective conservation, characterization and utilization of germplasm resources1. So far, few chickpea (Cicer arietinum) germplasm accessions have been characterized at the genome sequence level2. Here we present a detailed map of variation in 3,171 cultivated and 195 wild accessions to provide publicly available resources for chickpea genomics research and breeding. We constructed a chickpea pan-genome to describe genomic diversity across cultivated chickpea and its wild progenitor accessions. A divergence tree using genes present in around 80% of individuals in one species allowed us to estimate the divergence of Cicer over the last 21 million years. Our analysis found chromosomal segments and genes that show signatures of selection during domestication, migration and improvement. The chromosomal locations of deleterious mutations responsible for limited genetic diversity and decreased fitness were identified in elite germplasm. We identified superior haplotypes for improvement-related traits in landraces that can be introgressed into elite breeding lines through haplotype-based breeding, and found targets for purging deleterious alleles through genomics-assisted breeding and/or gene editing. Finally, we propose three crop breeding strategies based on genomic prediction to enhance crop productivity for 16 traits while avoiding the erosion of genetic diversity through optimal contribution selection (OCS)-based pre-breeding. The predicted performance for 100-seed weight, an important yield-related trait, increased by up to 23% and 12% with OCS- and haplotype-based genomic approaches, respectively.


Assuntos
Cicer/genética , Variação Genética , Genoma de Planta/genética , Análise de Sequência de DNA , Produtos Agrícolas/genética , Haplótipos/genética , Melhoramento Vegetal , Polimorfismo de Nucleotídeo Único/genética
2.
BMC Plant Biol ; 24(1): 124, 2024 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-38373874

RESUMO

BACKGROUND: Rice (Oryza sativa L.) is one of the globally important staple food crops, and yield-related traits are prerequisites for improved breeding efficiency in rice. Here, we used six different genome-wide association study (GWAS) models for 198 accessions, with 553,229 single nucleotide markers (SNPs) to identify the quantitative trait nucleotides (QTNs) and candidate genes (CGs) governing rice yield. RESULTS: Amongst the 73 different QTNs in total, 24 were co-localized with already reported QTLs or loci in previous mapping studies. We obtained fifteen significant QTNs, pathway analysis revealed 10 potential candidates within 100kb of these QTNs that are predicted to govern plant height, days to flowering, and plot yield in rice. Based on their superior allelic information in 20 elite and 6 inferior genotypes, we found a higher percentage of superior alleles in the elite genotypes in comparison to inferior genotypes. Further, we implemented expression analysis and enrichment analysis enabling the identification of 73 candidate genes and 25 homologues of Arabidopsis, 19 of which might regulate rice yield traits. Of these candidate genes, 40 CGs were found to be enriched in 60 GO terms of the studied traits for instance, positive regulator metabolic process (GO:0010929), intracellular part (GO:0031090), and nucleic acid binding (GO:0090079). Haplotype and phenotypic variation analysis confirmed that LOC_OS09G15770, LOC_OS02G36710 and LOC_OS02G17520 are key candidates associated with rice yield. CONCLUSIONS: Overall, we foresee that the QTNs, putative candidates elucidated in the study could summarize the polygenic regulatory networks controlling rice yield and be useful for breeding high-yielding varieties.


Assuntos
Estudo de Associação Genômica Ampla , Oryza , Mapeamento Cromossômico , Oryza/genética , Melhoramento Vegetal , Locos de Características Quantitativas/genética
3.
Plant Biotechnol J ; 22(6): 1504-1515, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38206288

RESUMO

Professor Rajeev K. Varshney's transformative impact on crop genomics, genetics, and agriculture is the result of his passion, dedication, and unyielding commitment to harnessing the potential of genomics to address the most pressing challenges faced by the global agricultural community. Starting from a small town in India and reaching the global stage, Professor Varshney's academic and professional trajectory has inspired many scientists active in research today. His ground-breaking work, especially his effort to list orphan tropical crops to genomic resource-rich entities, has been transformative. Beyond his scientific achievements, Professor Varshney is recognized by his colleagues as an exemplary mentor, fostering the growth of future researchers, building institutional capacity, and strengthening scientific capability. His focus on translational genomics and strengthening seed system in developing countries for the improvement of agriculture has made a tangible impact on farmers' lives. His skills have been best utilized in roles at leading research centres where he has applied his expertise to deliver a new vision for crop improvement. These efforts have now been recognized by the Royal Society with the award of the Fellowship (FRS). As we mark this significant milestone in his career, we not only celebrate Professor Varshney's accomplishments but also his wider contributions that continue to transform the agricultural landscape.


Assuntos
Produtos Agrícolas , Genômica , Retratos como Assunto , Agricultura/história , Produtos Agrícolas/genética , Genômica/história , História do Século XX , História do Século XXI , Retratos como Assunto , Sociedades Científicas/organização & administração
4.
Langmuir ; 40(14): 7412-7421, 2024 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-38529936

RESUMO

A quick evaluation of the effectiveness of additives is important in lubricant formulation. In this study, we employed a friction-force measurement approach using a newly designed lateral force-controlled tribometer. This tribometer evaluates the lubricant properties under boundary lubrication. In this lateral force-controlled tribometer, the absence of a stiffness-altering sensor enables the modeling of the actual contact conditions without altering the contact stiffness. Indirect friction force measurement ensures precise measurements of friction properties while avoiding common measurement errors encountered in conventional tribometers, such as sensor misalignment and changes in the stiffness of the machine due to the sensor. The tribometer designed and built consists of a pendulum that measures the rate of dissipation of the oscillation amplitude as a function of time. The unique characteristics of the machine are the possibility of changing the energy input into the tribosystem without altering the tribo-contact conditions and the capability to do experiments at higher temperatures. To evaluate the capabilities of this tribometer, the impact of temperature on the frictional properties of a base oil and a blend of base oil and stearic acid (SA) (a prominent Organic Friction Modifier) is investigated. The test result shows that frictional energy dissipation decreases when stearic acid is present in the lubricant. And, as the temperature of the oil increases, the energy dissipation increases for pure base oil but reduces for the blend. The observed frictional trends are attributed to the decrease in the viscosity of the base oil with an increase in the temperature. The decrease in friction for the SA blend is attributed to tribofilm formation. Fourier transform infrared (FTIR) and X-ray Photoelectron Spectroscopy (XPS) analyses confirm the presence of the SA tribofilm on the surface. XPS indicates an increase in tribofilm quantity with rising temperatures. The kinetics of film formation and thickness increase with temperature, consequently reducing the friction in the SA blend.

5.
Biologicals ; 87: 101784, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39053122

RESUMO

Pneumococcal conjugate vaccines (PCVs) have been developed to protect against pneumococcal diseases caused by the more than 100 serotypes of the bacterium Streptococcus pneumoniae. PCVs primarily prevent pneumococcal infections such as sepsis, bacteraemia, meningitis, otitis media, pneumonia, septicaemia, and sinusitis among infants, adults, elderly, and immunocompromised individuals. The current available PCVs only cover a limited number of serotypes, and there is an immense need for developing higher-valent PCVs that can protect against non-vaccine serotypes to overcome challenges like serotype replacement and antibiotic resistance. The main challenges for developing higher valent PCVs are the complexity of the manufacturing process comprising polysaccharide fermentation, purification, modification or sizing of multiple polysaccharides and conjugation between polysaccharides and carrier proteins, the stability of the conjugates, and the immunogenicity of the vaccine. Different manufacturing processes have been explored to produce higher valent PCVs using different serotypes of S. pneumoniae and conjugation with different carrier proteins. The global coverage of higher valent PCVs are still low, mainly due to the high cost and limited supply of the vaccine. This review focuses on the existing and emerging manufacturing processes and challenges associated with higher-valent pneumococcal PCV development.


Assuntos
Infecções Pneumocócicas , Vacinas Pneumocócicas , Streptococcus pneumoniae , Vacinas Conjugadas , Vacinas Pneumocócicas/imunologia , Vacinas Pneumocócicas/química , Vacinas Pneumocócicas/uso terapêutico , Vacinas Conjugadas/imunologia , Vacinas Conjugadas/química , Humanos , Streptococcus pneumoniae/imunologia , Infecções Pneumocócicas/prevenção & controle , Infecções Pneumocócicas/microbiologia , Infecções Pneumocócicas/imunologia
7.
Theor Appl Genet ; 134(6): 1829-1843, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34014373

RESUMO

KEY MESSAGE: Integrating genomics technologies and breeding methods to tweak core parameters of the breeder's equation could accelerate delivery of climate-resilient and nutrient rich crops for future food security. Accelerating genetic gain in crop improvement programs with respect to climate resilience and nutrition traits, and the realization of the improved gain in farmers' fields require integration of several approaches. This article focuses on innovative approaches to address core components of the breeder's equation. A prerequisite to enhancing genetic variance (σ2g) is the identification or creation of favorable alleles/haplotypes and their deployment for improving key traits. Novel alleles for new and existing target traits need to be accessed and added to the breeding population while maintaining genetic diversity. Selection intensity (i) in the breeding program can be improved by testing a larger population size, enabled by the statistical designs with minimal replications and high-throughput phenotyping. Selection priorities and criteria to select appropriate portion of the population too assume an important role. The most important component of breeder's equation is heritability (h2). Heritability estimates depend on several factors including the size and the type of population and the statistical methods. The present article starts with a brief discussion on the potential ways to enhance σ2g in the population. We highlight statistical methods and experimental designs that could improve trait heritability estimation. We also offer a perspective on reducing the breeding cycle time (t), which could be achieved through the selection of appropriate parents, optimizing the breeding scheme, rapid fixation of target alleles, and combining speed breeding with breeding programs to optimize trials for release. Finally, we summarize knowledge from multiple disciplines for enhancing genetic gains for climate resilience and nutritional traits.


Assuntos
Mudança Climática , Produtos Agrícolas/genética , Genômica , Valor Nutritivo , Melhoramento Vegetal , Alelos , Edição de Genes , Interação Gene-Ambiente , Genes de Plantas , Variação Genética , Fenótipo , Seleção Genética
8.
Br J Clin Pharmacol ; 87(11): 4366-4374, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-33856070

RESUMO

AIMS: The current opioid crisis in the USA is a formidable challenge for the healthcare system, and the general population. Our objective is to characterize the burden of opioid-related disorders in an inpatient setting in the USA for the years 2016, 2017 and 2018 using the National Inpatient Sample (NIS). METHODS: A cross-sectional analysis of the NIS was performed to identify and analyse hospitalizations with an opioid-related diagnosis in 2016, 2017 and 2018. Descriptive statistics and regression models were utilized to define the demographics of the population of interest and measure the outcomes. RESULTS: We identified 962 900 discharges with opioid-related diagnosis in 2016, 982 710 in 2017 and 942 110 in 2018. The majority were age <60 years, were found in residents of low-income zip codes and covered by Medicaid. The adjusted mean total hospitalization cost trended up from $12 828 (95% confidence interval [CI] 12 547-13 108) in 2016, to $13164.9 (95% CI 12 872.47-13 457.34) in 2017 and then to $13 626.65 (95% CI 13 325.95-13 927.34) in 2018. The adjusted mortality was highest in 2016; 2.26% (95% CI 2.16-2.35) and it trended down to 1.97% (95% CI 1.88-2.05) in 2017, and to 1.89% (95% CI 1.81-1.98) in 2018. CONCLUSIONS: Opioid-related disorders cause a significant number of hospitalizations in the USA. A large proportion of these patients are age <60 years, have lower household income, and are covered by Medicaid. Programmes directed towards this specific group can help reduce the overall burden of hospitalizations.


Assuntos
Analgésicos Opioides , Transtornos Relacionados ao Uso de Opioides , Analgésicos Opioides/efeitos adversos , Estudos Transversais , Hospitalização , Humanos , Pacientes Internados , Pessoa de Meia-Idade , Transtornos Relacionados ao Uso de Opioides/tratamento farmacológico , Transtornos Relacionados ao Uso de Opioides/epidemiologia , Estados Unidos/epidemiologia
9.
Funct Integr Genomics ; 20(6): 739-761, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33089419

RESUMO

Epigenetics is defined as changes in gene expression that are not associated with changes in DNA sequence but due to the result of methylation of DNA and post-translational modifications to the histones. These epigenetic modifications are known to regulate gene expression by bringing changes in the chromatin state, which underlies plant development and shapes phenotypic plasticity in responses to the environment and internal cues. This review articulates the role of histone modifications and DNA methylation in modulating biotic and abiotic stresses, as well as crop improvement. It also highlights the possibility of engineering epigenomes and epigenome-based predictive models for improving agronomic traits.


Assuntos
Epigenômica/tendências , Código das Histonas/genética , Histonas/genética , Melhoramento Vegetal , Cromatina/genética , Produtos Agrícolas/genética , Metilação de DNA/genética , Regulação da Expressão Gênica de Plantas/genética , Desenvolvimento Vegetal/genética , Plantas/genética , Processamento de Proteína Pós-Traducional/genética
10.
Plant Biotechnol J ; 18(12): 2482-2490, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32455481

RESUMO

Haplotype-based breeding, a recent promising breeding approach to develop tailor-made crop varieties, deals with identification of superior haplotypes and their deployment in breeding programmes. In this context, whole genome re-sequencing data of 292 genotypes from pigeonpea reference set were mined to identify the superior haplotypes for 10 drought-responsive candidate genes. A total of 83, 132 and 60 haplotypes were identified in breeding lines, landraces and wild species, respectively. Candidate gene-based association analysis of these 10 genes on a subset of 137 accessions of the pigeonpea reference set revealed 23 strong marker-trait associations (MTAs) in five genes influencing seven drought-responsive component traits. Haplo-pheno analysis for the strongly associated genes resulted in the identification of most promising haplotypes for three genes regulating five component drought traits. The haplotype C. cajan_23080-H2 for plant weight (PW), fresh weight (FW) and turgid weight (TW), the haplotype C. cajan_30211-H6 for PW, FW, TW and dry weight (DW), the haplotype C. cajan_26230-H11 for FW and DW and the haplotype C. cajan_26230-H5 for relative water content (RWC) were identified as superior haplotypes under drought stress condition. Furthermore, 17 accessions containing superior haplotypes for three drought-responsive genes were identified. The identified superior haplotypes and the accessions carrying these superior haplotypes will be very useful for deploying haplotype-based breeding to develop next-generation tailor-made better drought-responsive pigeonpea cultivars.


Assuntos
Cajanus , Cruzamento , Secas , Genótipo , Haplótipos
11.
Plant Biotechnol J ; 18(8): 1697-1710, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-31925873

RESUMO

Hybrids are extensively used in agriculture to deliver an increase in yield, yet the molecular basis of heterosis is not well understood. Global DNA methylation analysis, transcriptome analysis and small RNA profiling were aimed to understand the epigenetic effect of the changes in gene expression level in the two hybrids and their parental lines. Increased DNA methylation was observed in both the hybrids as compared to their parents. This increased DNA methylation in hybrids showed that majority of the 24-nt siRNA clusters had higher expression in hybrids than the parents. Transcriptome analysis revealed that various phytohormones (auxin and salicylic acid) responsive hybrid-MPV DEGs were significantly altered in both the hybrids in comparison to MPV. DEGs associated with plant immunity and growth were overexpressed whereas DEGs associated with basal defence level were repressed. This antagonistic patterns of gene expression might contribute to the greater growth of the hybrids. It was also noticed that some common as well as unique changes in the regulatory pathways were associated with heterotic growth in both the hybrids. Approximately 70% and 67% of down-regulated hybrid-MPV DEGs were found to be differentially methylated in ICPH 2671 and ICPH 2740 hybrid, respectively. This reflected the association of epigenetic regulation in altered gene expressions. Our findings also revealed that miRNAs might play important roles in hybrid vigour in both the hybrids by regulating their target genes, especially in controlling plant growth and development, defence and stress response pathways. The above finding provides an insight into the molecular mechanism of pigeonpea heterosis.


Assuntos
Epigênese Genética , Vigor Híbrido , Metilação de DNA/genética , Epigênese Genética/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas/genética , Genoma de Planta , Vigor Híbrido/genética
12.
Mol Genet Genomics ; 294(1): 57-68, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30173295

RESUMO

Pigeonpea is an important source of dietary protein to over a billion people globally, but genetic enhancement of seed protein content (SPC) in the crop has received limited attention for a long time. Use of genomics-assisted breeding would facilitate accelerating genetic gain for SPC. However, neither genetic markers nor genes associated with this important trait have been identified in this crop. Therefore, the present study exploited whole genome re-sequencing (WGRS) data of four pigeonpea genotypes (~ 12X coverage) to identify sequence-based markers and associated candidate genes for SPC. By combining a common variant filtering strategy on available WGRS data with knowledge of gene functions in relation to SPC, 108 sequence variants from 57 genes were identified. These genes were assigned to 19 GO molecular function categories with 56% belonging to only two categories. Furthermore, Sanger sequencing confirmed presence of 75.4% of the variants in 37 genes. Out of 30 sequence variants converted into CAPS/dCAPS markers, 17 showed high level of polymorphism between low and high SPC genotypes. Assay of 16 of the polymorphic CAPS/dCAPS markers on an F2 population of the cross ICP 5529 (high SPC) × ICP 11605 (low SPC), resulted in four of the CAPS/dCAPS markers significantly (P < 0.05) co-segregated with SPC. In summary, four markers derived from mutations in four genes will be useful for enhancing/regulating SPC in pigeonpea crop improvement programs.


Assuntos
Cajanus/genética , Marcadores Genéticos , Sementes/genética , Sequenciamento Completo do Genoma/métodos , Cajanus/metabolismo , Mapeamento Cromossômico , DNA de Plantas/genética , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Mutação , Fenótipo , Melhoramento Vegetal , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sementes/metabolismo
13.
Theor Appl Genet ; 132(3): 797-816, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30560464

RESUMO

Efficiency of breeding programs of legume crops such as chickpea, pigeonpea and groundnut has been considerably improved over the past decade through deployment of modern genomic tools and technologies. For instance, next-generation sequencing technologies have facilitated availability of genome sequence assemblies, re-sequencing of several hundred lines, development of HapMaps, high-density genetic maps, a range of marker genotyping platforms and identification of markers associated with a number of agronomic traits in these legume crops. Although marker-assisted backcrossing and marker-assisted selection approaches have been used to develop superior lines in several cases, it is the need of the hour for continuous population improvement after every breeding cycle to accelerate genetic gain in the breeding programs. In this context, we propose a sequence-based breeding approach which includes use of independent or combination of parental selection, enhancing genetic diversity of breeding programs, forward breeding for early generation selection, and genomic selection using sequencing/genotyping technologies. Also, adoption of speed breeding technology by generating 4-6 generations per year will be contributing to accelerate genetic gain. While we see a huge potential of the sequence-based breeding to revolutionize crop improvement programs in these legumes, we anticipate several challenges especially associated with high-quality and precise phenotyping at affordable costs, data analysis and management related to improving breeding operation efficiency. Finally, integration of improved seed systems and better agronomic packages with the development of improved varieties by using sequence-based breeding will ensure higher genetic gains in farmers' fields.


Assuntos
Fabaceae/genética , Genoma de Planta , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Melhoramento Vegetal/métodos , Técnicas de Genotipagem , Característica Quantitativa Herdável
14.
Int J Neurosci ; 129(7): 722-728, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30526175

RESUMO

Oligodendrogliomas are a rare type of primary brain tumor. They are genetically defined as diffuse gliomas carrying mutation in isocitrate dehydrogenase type 1 (IDH1) or type 2 (IDH2) and codeletion of chromosomes 1p and 19q. The WHO grading system distinguishes two histopathologic grades of ODs: grade II (low-grade) and grade III (anaplastic oligodendroglioma or AO). These tumors rarely metastasize outside of central nervous system with only few cases reported in the literature. Here we present a case of an AO, which metastasized to the bone marrow and other sites within a year of diagnosis despite aggressive treatment measures. Our patient eventually succumbed to his disease, raising many questions about this rare condition, its natural course and optimal management strategy.


Assuntos
Neoplasias da Medula Óssea/secundário , Neoplasias Encefálicas/patologia , Oligodendroglioma/patologia , Neoplasias da Medula Óssea/diagnóstico por imagem , Encéfalo/diagnóstico por imagem , Encéfalo/patologia , Neoplasias Encefálicas/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Oligodendroglioma/diagnóstico por imagem
15.
Plant Biotechnol J ; 15(7): 906-914, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28027425

RESUMO

Identification of candidate genomic regions associated with target traits using conventional mapping methods is challenging and time-consuming. In recent years, a number of single nucleotide polymorphism (SNP)-based mapping approaches have been developed and used for identification of candidate/putative genomic regions. However, in the majority of these studies, insertion-deletion (Indel) were largely ignored. For efficient use of Indels in mapping target traits, we propose Indel-seq approach, which is a combination of whole-genome resequencing (WGRS) and bulked segregant analysis (BSA) and relies on the Indel frequencies in extreme bulks. Deployment of Indel-seq approach for identification of candidate genomic regions associated with fusarium wilt (FW) and sterility mosaic disease (SMD) resistance in pigeonpea has identified 16 Indels affecting 26 putative candidate genes. Of these 26 affected putative candidate genes, 24 genes showed effect in the upstream/downstream of the genic region and two genes showed effect in the genes. Validation of these 16 candidate Indels in other FW- and SMD-resistant and FW- and SMD-susceptible genotypes revealed a significant association of five Indels (three for FW and two for SMD resistance). Comparative analysis of Indel-seq with other genetic mapping approaches highlighted the importance of the approach in identification of significant genomic regions associated with target traits. Therefore, the Indel-seq approach can be used for quick and precise identification of candidate genomic regions for any target traits in any crop species.


Assuntos
Cajanus/genética , Genoma de Planta/genética , Cajanus/microbiologia , Fusarium/patogenicidade , Genótipo , Mutação INDEL/genética , Polimorfismo de Nucleotídeo Único/genética
16.
Plant Biotechnol J ; 15(8): 927-941, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28028892

RESUMO

Rust and late leaf spot (LLS) are the two major foliar fungal diseases in groundnut, and their co-occurrence leads to significant yield loss in addition to the deterioration of fodder quality. To identify candidate genomic regions controlling resistance to rust and LLS, whole-genome resequencing (WGRS)-based approach referred as 'QTL-seq' was deployed. A total of 231.67 Gb raw and 192.10 Gb of clean sequence data were generated through WGRS of resistant parent and the resistant and susceptible bulks for rust and LLS. Sequence analysis of bulks for rust and LLS with reference-guided resistant parent assembly identified 3136 single-nucleotide polymorphisms (SNPs) for rust and 66 SNPs for LLS with the read depth of ≥7 in the identified genomic region on pseudomolecule A03. Detailed analysis identified 30 nonsynonymous SNPs affecting 25 candidate genes for rust resistance, while 14 intronic and three synonymous SNPs affecting nine candidate genes for LLS resistance. Subsequently, allele-specific diagnostic markers were identified for three SNPs for rust resistance and one SNP for LLS resistance. Genotyping of one RIL population (TAG 24 × GPBD 4) with these four diagnostic markers revealed higher phenotypic variation for these two diseases. These results suggest usefulness of QTL-seq approach in precise and rapid identification of candidate genomic regions and development of diagnostic markers for breeding applications.


Assuntos
Arachis/genética , Genoma de Planta/genética , Doenças das Plantas/genética , Folhas de Planta/genética , Alelos , Arachis/microbiologia , Genótipo , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Polimorfismo de Nucleotídeo Único/genética , Locos de Características Quantitativas/genética
17.
Plant Biotechnol J ; 14(11): 2110-2119, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27107184

RESUMO

Terminal drought is a major constraint to chickpea productivity. Two component traits responsible for reduction in yield under drought stress include reduction in seeds size and root length/root density. QTL-seq approach, therefore, was used to identify candidate genomic regions for 100-seed weight (100SDW) and total dry root weight to total plant dry weight ratio (RTR) under rainfed conditions. Genomewide SNP profiling of extreme phenotypic bulks from the ICC 4958 × ICC 1882 population identified two significant genomic regions, one on CaLG01 (1.08 Mb) and another on CaLG04 (2.7 Mb) linkage groups for 100SDW. Similarly, one significant genomic region on CaLG04 (1.10 Mb) was identified for RTR. Comprehensive analysis revealed four and five putative candidate genes associated with 100SDW and RTR, respectively. Subsequently, two genes (Ca_04364 and Ca_04607) for 100SDW and one gene (Ca_04586) for RTR were validated using CAPS/dCAPS markers. Identified candidate genomic regions and genes may be useful for molecular breeding for chickpea improvement.


Assuntos
Cicer/genética , Locos de Características Quantitativas/genética , Cruzamento , Cicer/metabolismo , Cicer/fisiologia , Polimorfismo de Nucleotídeo Único/genética
18.
Plant Biotechnol J ; 14(5): 1183-94, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26397045

RESUMO

To map resistance genes for Fusarium wilt (FW) and sterility mosaic disease (SMD) in pigeonpea, sequencing-based bulked segregant analysis (Seq-BSA) was used. Resistant (R) and susceptible (S) bulks from the extreme recombinant inbred lines of ICPL 20096 × ICPL 332 were sequenced. Subsequently, SNP index was calculated between R- and S-bulks with the help of draft genome sequence and reference-guided assembly of ICPL 20096 (resistant parent). Seq-BSA has provided seven candidate SNPs for FW and SMD resistance in pigeonpea. In parallel, four additional genotypes were re-sequenced and their combined analysis with R- and S-bulks has provided a total of 8362 nonsynonymous (ns) SNPs. Of 8362 nsSNPs, 60 were found within the 2-Mb flanking regions of seven candidate SNPs identified through Seq-BSA. Haplotype analysis narrowed down to eight nsSNPs in seven genes. These eight nsSNPs were further validated by re-sequencing 11 genotypes that are resistant and susceptible to FW and SMD. This analysis revealed association of four candidate nsSNPs in four genes with FW resistance and four candidate nsSNPs in three genes with SMD resistance. Further, In silico protein analysis and expression profiling identified two most promising candidate genes namely C.cajan_01839 for SMD resistance and C.cajan_03203 for FW resistance. Identified candidate genomic regions/SNPs will be useful for genomics-assisted breeding in pigeonpea.


Assuntos
Cajanus/genética , Fusarium/fisiologia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Doenças das Plantas/imunologia , Polimorfismo de Nucleotídeo Único/genética , Cruzamento , Cajanus/imunologia , Mapeamento Cromossômico , Resistência à Doença/genética , Genótipo , Infertilidade das Plantas/genética , Análise de Sequência de DNA
19.
Theor Appl Genet ; 128(6): 999-1017, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25855139

RESUMO

KEY MESSAGE: MAGIC populations present novel challenges and opportunities in crops due to their complex pedigree structure. They offer great potential both for dissecting genomic structure and for improving breeding populations. The past decade has seen the rise of multiparental populations as a study design offering great advantages for genetic studies in plants. The genetic diversity of multiple parents, recombined over several generations, generates a genetic resource population with large phenotypic diversity suitable for high-resolution trait mapping. While there are many variations on the general design, this review focuses on populations where the parents have all been inter-mated, typically termed Multi-parent Advanced Generation Intercrosses (MAGIC). Such populations have already been created in model animals and plants, and are emerging in many crop species. However, there has been little consideration of the full range of factors which create novel challenges for design and analysis in these populations. We will present brief descriptions of large MAGIC crop studies currently in progress to motivate discussion of population construction, efficient experimental design, and genetic analysis in these populations. In addition, we will highlight some recent achievements and discuss the opportunities and advantages to exploit the unique structure of these resources post-QTL analysis for gene discovery.


Assuntos
Cruzamento , Produtos Agrícolas/genética , Cruzamentos Genéticos , Variação Genética , Agricultura/métodos , Mapeamento Cromossômico , Epistasia Genética , Ligação Genética , Genótipo , Fenótipo , Locos de Características Quantitativas
20.
Plant J ; 74(5): 715-29, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23489434

RESUMO

Cicer arietinum L. (chickpea) is the third most important food legume crop. We have generated the draft sequence of a desi-type chickpea genome using next-generation sequencing platforms, bacterial artificial chromosome end sequences and a genetic map. The 520-Mb assembly covers 70% of the predicted 740-Mb genome length, and more than 80% of the gene space. Genome analysis predicts the presence of 27,571 genes and 210 Mb as repeat elements. The gene expression analysis performed using 274 million RNA-Seq reads identified several tissue-specific and stress-responsive genes. Although segmental duplicated blocks are observed, the chickpea genome does not exhibit any indication of recent whole-genome duplication. Nucleotide diversity analysis provides an assessment of a narrow genetic base within the chickpea cultivars. We have developed a resource for genetic markers by comparing the genome sequences of one wild and three cultivated chickpea genotypes. The draft genome sequence is expected to facilitate genetic enhancement and breeding to develop improved chickpea varieties.


Assuntos
Cicer/genética , Genoma de Planta , Análise de Sequência de DNA/métodos , Transcriptoma/genética , Composição de Bases/genética , Mapeamento Cromossômico , Cromossomos de Plantas/genética , Fabaceae/classificação , Fabaceae/genética , Variação Genética , Genótipo , Repetições de Microssatélites/genética , Filogenia , Proteínas de Plantas/classificação , Proteínas de Plantas/genética , Sintenia
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