Water consumption in Iron Age, Roman, and Early Medieval Croatia.

Patterns of water consumption by past human populations are rarely considered, yet drinking behavior is socially mediated and access to water sources is often socially controlled. Oxygen isotope analysis of archeological human remains is commonly used to identify migrants in the archeological record, but it can also be used to consider water itself, as this technique documents water consumption rather than migration directly. Here, we report an oxygen isotope study of humans and animals from coastal regions of Croatia in the Iron Age, Roman, and Early Medieval periods. The results show that while faunal values have little diachronic variation, the human data vary through time, and there are wide ranges of values within each period. Our interpretation is that this is not solely a result of mobility, but that human behavior can and did lead to human oxygen isotope ratios that are different from that expected from consumption of local precipitation.

Bone growth, limb proportions and non-specific stress in archaeological populations from Croatia.

BACKGROUND: The effect of environmental factors and, in particular, non-specific stress on the growth patterns of limbs and other body dimensions of children from past populations is not well understood. AIMS: This study assesses whether growth of mediaeval and post-mediaeval children aged between 0-11.5 years from Adriatic (coastal) and continental Croatia varies by region and by the prevalence and type of non-specific stress. METHODS: Dental ages were estimated using the Moorrees, Fanning and Hunt (MFH) scoring method. Growth of long bone diaphyses (femur, tibia, humerus, radius and ulna) was assessed by using a composite Z-score statistic (CZS). Clavicular length was measured as a proxy for upper trunk width, distal metaphyseal width of the femur was measured as a proxy for body mass and upper and lower intra-limb indices were calculated. Differences between sub-sets sampled by (a) region and (b) active vs healed non-specific stress indicators and (c) intra-limb indices were tested by Mann--Whitney U-tests and Analysis of Covariance (ANCOVA). RESULTS: Adriatic children attained larger dimensions-per-age than continental children. Children with healed stress lesions had larger dimensions-per-age than those with active lesions. No inter-regional difference was found in intra-limb indices. CONCLUSION: These findings highlight the complexity of growth patterns in past populations and indicate that variation in environmental conditions such as diet and differences in the nature of non-specific stress lesions both exert a significant effect on long bone growth.

Changing cultures, changing cuisines: Cultural transitions and dietary change in Iron Age, Roman, and Early Medieval Croatia.

Food is well-known to encode social and cultural values, for example different social groups use different consumption patterns to act as social boundaries. When societies and cultures change, whether through drift, through population replacement or other factors, diet may also alter despite unchanging resource availability within a region. This study investigates the extent to which dietary change coincides with cultural change, to understand the effects of large-scale migrations on the populations' diets. Through stable carbon and nitrogen isotope analysis of Iron Age, Roman, and Early Medieval human bone collagen, we show that in Croatia large-scale cultural change led to significant changes in diet. The isotopic evidence indicates that Iron Age diet consisted of C(3) foodstuffs with no isotopic evidence for the consumption of C(4) or marine resources. With the Roman conquest, marine resources were added to the diet, although C(3) foodstuffs continued to play an important role. In the Early Medieval period, this marine component was lost and varying amounts of C(4) foodstuffs, probably millet, were added to the otherwise C(3) diet. In both of these transitions it is likely that the changes in diet are related to the arrival of a new people into the area.

Life stress on the Roman limes in continental Croatia.

The purpose of the paper is to analyze and compare the demographic profiles and disease frequencies between a skeletal series from Zmajevac, a settlement on the Danubian limes, and a composite "non-limes" skeletal series consisting of human osteological remains from three large urban settlements to the west of the limes; roman Mursa (modern Osijek), Cibalae (Vinkovci) and Certissia (Strbinci). To determine if life stresses were different in settlements on the limes the age and sex distribution in Zmajevac was compared to the composite "non-limes" series. All skeletons were also analyzed for the presence of dental pathology, dental enamel hypoplasia, cribra orbitalia, trauma, and physical stress. Data collected from the skeletal series show that, with the exception of some indicators of physical stress, no significant differences in quality of life is evident. Both series are characterized by an under-representation of subadults from the youngest age category and by similar average adult male and female ages at death. In Zmajevac the average ages at death for adult males and females were 40.0 and 39.0 years respectively, in the composite "non-limes" series 37.4 years for both males and females. The frequencies of dental disease, subadult stress indicators, and trauma are similar in both series. The only consistent difference between the two series is noted in the frequencies of skeletal markers of physical stress, in particular the frequencies of vertebral osteoarthritis and Schmorl's defects. Total male and total female vertebral osteoarthritis frequencies in the two series are significantly different, as is the difference in total male frequencies of Schmorl's defects. Young adult males in the Zmajevac series seem to have been experiencing particularly heavy physical strain on the vertebral column. They exhibit significantly higher frequencies of both vertebral osteoarthritis and Schmorl's defects than young adult males from the composite non-limes series.

Dental disease in the late Medieval population from Nova Raca, Croatia.

Male and female dental disease frequencies were compared to test the hypothesis of differential stress levels between the sexes in the late Medieval population from Nova Raca, Croatia. The following categories of dental disease were compared: alveolar bone pathology defined by the presence of periodontal or periapical abscesses or antemortem tooth loss, caries, hypoplastic defects in the enamel and occlusal surface wear. The results show clear gender differences in the frequencies of alveolar bone pathology, carious lesions and occlusal surface wear. In all cases males exhibited significantly greater frequencies. Further analyses indicated that these differences were related to younger (15-29 years) adults and to the upper jaw. Together with paleodemographic data and the fact that females exhibited higher frequencies of enamel defects, this dichotomy in dental disease frequencies is interpreted as the result of a culturally imposed defensive mechanism which selectively buffered young males from malnutrition, exposing them at the same time to higher levels of cariogenic food and thus to higher frequencies of carious lesions and alveolar bone disease.

Carabelli's trait in Croatian populations over 1800 years.

Examination and comparison of the morphological features of tooth crown in archaeological and recent samples can be difficult due to the different levels of tooth wear seen both within and between populations. These differences make the comparison of frequency data for Carabelli trait problematic. The aim of the present study is to detect the frequency and degree of expression of Carabelli's trait in Croatian populations from late antiquity to recent times and to use these data as supplementary evidence of complex population migration. A total of 1287 individuals from the late antiquity, medieval, early modern and modern periods were examined. Correlation between the presence of Carabelli's trait and tooth crown size was tested. The results of our analyses show that the frequency of Carabelli's trait is significantly greater in the early modern period (51.3%) and in the 21st century (43.1%) than in the late antiquity (20.4%) and medieval periods (23.4%). These results are consistent with historical evidence of migration and population change in the territory of present-day Croatia throughout the almost 1800 years covered by this study. The results also provide additional evidence for the complex nature of population change in the transition from the late antiquity to the early medieval period.

Skeletal and dental indicators of health in the late mediaeval (12-15th century) population from Nin, southern Croatia.

A comprehensive bioarchaeological study of the late mediaeval (12-15th century) skeletal sample from Nin was carried out in order to test the historically documented hypothesis that during the late mediaeval period Nin sustained a period of rapid development that resulted in it becoming one of the major urban centres on the eastern Adriatic coast. The analysed pathological changes (alveolar bone disease, dental caries, dental enamel hypoplasia, cribra orbitalia, periostitis, tuberculosis, Schmorl's nodes, vertebral osteoarthritis, and bone fractures) indicate a relatively good quality of life for the majority of the population from this late mediaeval site. A low prevalence of dental pathologies suggests an adequate diet while a low frequency of long bone trauma testifies to a relatively peaceful life for the inhabitants of mediaeval Nin. Increased urban development during this period resulted in a worsening of sanitary conditions most likely caused by overcrowding, which is reflected in the presence of tuberculosis and the relatively high frequencies of dental enamel hypoplasia and cribra orbitalia. An additional health concern for the late mediaeval inhabitants of Nin may have been the presence of malaria, as recorded in numerous historical sources. Comparison with other Croatian mediaeval skeletal samples suggests that the inhabitants of late mediaeval Nin experienced somewhat better living conditions than their contemporaries from other parts of Croatia.

Biocultural analysis of sex differences in mortality profiles and stress levels in the late medieval population from Nova Raca, Croatia.

Human skeletal remains of 104 individuals from the late medieval (14th-18th century) Nova Raca cemetery, in continental Croatia, are described. Historic data from the parish Book of the Dead, relevant to a period in the early 19th century, suggest that females may have been under greater stress than males. To test this hypothesis, the skeletal material is analyzed for the presence and distribution of enamel hypoplasias and cribra orbitalia. Observations are also made on the presence and pattern of dental disease, skeletal infection, trauma, osteoarthritis, vertebral degenerative changes, and Schmorl's depression frequencies. The mortuary sample, consisting of 36 subadults and 68 adults, exhibits underrepresentation of subadults, and differential adult male/female mortality profiles. Peak female mortality is between 21-25 years, compared to peak male mortality between 31-35 years. Average adult female age at death is 29.9 years, compared to 34.1 years in adult males. Significant sex differences are present in the frequencies of enamel hypoplasia. Adult females have higher frequencies of hypoplastic teeth, and a larger number of defects in the teeth affected than in males. Subadults in the series also exhibit higher frequencies of hypoplastic teeth, and a larger number of defects in the teeth affected than in adults, documenting a relationship between enamel hypoplasia-causing stress events and reduced life expectancy. Significant sex differences are also present in dental pathology frequencies, possibly reflecting differences in resource access. Sex differences in vertebral osteoarthritis and Schmorl's depression frequencies may reflect differential activity patterns.

Osteochondroma in a skeleton from an 11th century Croatian cemetery.

We present a case of a well-preserved bone tumor in a skeleton from a Croatian skeletal series dated to the 11th century AD. The tumor is located on the anterior side of the neck of the right femur. The gross morphology of the tumor - a round, lumpy, cauliflower-like appearance with a fairly smooth external surface - is consistent with osteochondroma. The diagnosis is supported by x-ray and CAT-scan findings, which show thickened trabeculae and an internal structure of cancellous bone interspersed with areas of dense compact bone. Comparison with x-rays from a patient surgically treated in 1999 for an osteochondroma with the same localization shows that the characteristics of the tumor have remained unchanged from the 11th century.

Dental identification of war victims from Petrinja in Croatia.

In this paper the authors report their experiences and problems encountered in the identification of war victims from Petrinja in Croatia. Soon after Croatian forces regained Petrinja in 1995, four mass graves were discovered from which the bodies of 46 civilians, 38 males and 8 females, were recovered. Identification of the victims was performed at the Department of Forensic Medicine and Criminology at the School of Medicine in Zagreb. A forensic odonto-stomatologist from the Department of Dental Anthropology of the School of Dental Medicine at the University of Zagreb participated in the identification process by carrying out dental identifications. A total of 27 victims (59%) were identified, while 19 (41%) are at present still unidentified. Identification by supportive and anthropological evidence (e.g. sex, age, height, personal documents, dress, jewellery) was achieved in 43% of cases, while identification based only on dental records was achieved in 16%. The most useful dental characteristics for the purpose of identification were fixed and removable prosthetic appliances for oral rehabilitation. The reason for the low number of dental identifications was the lack of antemortem dental data which could be compared with postmortem dental records.