RESUMO
INTRODUCTION: While acute models of orthotopic lung transplantation have been described in dogs, the technical considerations of developing a survival model in this species have not been elaborated. Herein, we describe optimization of a canine survival model of orthotopic lung transplantation. METHODS: Protocols of orthotopic left lung transplantation and single lung ventilation were established in acute experiments (n=9). Four dogs, serving as controls, received autologous, orthotopic lung transplants. Allogeneic transplants were performed in 16 DLA-identical and 16 DLA-mismatched unrelated recipient dogs. Selective right lung ventilation was utilized in all animals. A Malecot tube was left in the pleural space connected to a Heimlich valve for up to 24 hours. To date, animals have been followed up to 24 months by chest radiography, pulmonary function tests, bronchoscopy with lavage, and open biopsies. RESULTS: Long-term survival was achieved in 34/36 animals. Two recipients died intraoperatively secondary to cardiac arrest. All animals were extubated on the operating table, and in all cases the chest tube was removed within 24 hours. Major complications included thrombosis of the pulmonary artery and subcritical stenosis of bronchial anastamosis. One recipient underwent successful treatment of a small bowel intussusception. CONCLUSIONS: We report our experience in developing a survival canine model of orthotopic single lung transplantation. While short-term survival following canine lung transplantation is achievable, we report particular considerations that facilitate animal comfort, early extubation, and lung reexpansion in the immediate postoperative period, further optimizing use of this species for experimental modeling of long-term complications after lung transplantation.
Assuntos
Sobrevivência de Enxerto/fisiologia , Transplante de Pulmão/fisiologia , Animais , Cães , Sobrevivência de Enxerto/imunologia , Transplante de Pulmão/imunologia , Transplante de Pulmão/veterinária , Modelos Animais , Fatores de Tempo , Coleta de Tecidos e Órgãos/métodos , Coleta de Tecidos e Órgãos/veterinária , Obtenção de Tecidos e Órgãos/métodos , Transplante Autólogo , Transplante HomólogoRESUMO
Endodontically treated multirooted posterior teeth can be predictably and conservatively restored by using a combination of cast cores and multiple narrow-diameter, parallel serrated stainless steel posts.
Assuntos
Coroas , Planejamento de Dentadura , Técnica para Retentor Intrarradicular , Técnica de Fundição Odontológica , Humanos , Tratamento do Canal Radicular/métodos , Aço InoxidávelRESUMO
A passive fit between osseointegrated implants and the prosthesis they will support has been advocated. An experimental model was developed to test the accuracy of three impression techniques and the components used to make the transfer records. Statistically, no significant difference was found between the three methods tested. From this initial study, it appears that further work is needed to isolate techniques that will predictably provide accurate registration of the position of endosseous implants.