A Short Review of the Toxicity of Dentifrices-Zebrafish Model as a Useful Tool in Ecotoxicological Studies.

This review aims to summarize the literature data regarding the effects of different toothpaste compounds in the zebrafish model. Danio rerio provides an insight into the mechanisms of the ecotoxicity of chemicals as well as an assessment of their fate in the environment to determine long-term environmental impact. The regular use of adequate toothpaste with safe active ingredients possessing anti-bacterial, anti-inflammatory, anti-oxidant, and regenerative properties is one of the most effective strategies for oral healthcare. In addition to water, a typical toothpaste consists of a variety of components, among which three are of predominant importance, i.e., abrasive substances, fluoride, and detergents. These ingredients provide healthy teeth, but their environmental impact on living organisms are often not well-known. Each of them can influence a higher level of organization: subcellular, cellular, tissue, organ, individual, and population. Therefore, it is very important that the properties of a chemical are detected before it is released into the environment to minimize damage. An important part of a chemical risk assessment is the estimation of the ecotoxicity of a compound. The zebrafish model has unique advantages in environmental ecotoxicity research and has been used to study vertebrate developmental biology. Among others, the advantages of this model include its external, visually accessible development, which allows for providing many experimental manipulations. The zebrafish has a significant genetic similarity with other vertebrates. Nevertheless, translating findings from zebrafish studies to human risk assessment requires careful consideration of these differences.

Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model.

Tobacco smoking is one of the most serious health problems. Potentially lethal effects of nicotine for adults can occur with as little as 30 to 60 mg, although severe symptoms can arise with lower doses. Furthermore, the route of administration also influences the toxicity. Cytisine is one of the most popular medications in nicotinism treatment. Like nicotine, cytisine is a plant alkaloid, signaling through nicotinic acetylcholine receptors. Our study evaluated the effects of cytisine in nicotine-induced embryotoxic effects using zebrafish larvae. We examined the teratogenicity of nicotine and cytisine alone or in combination. Nicotine increased mortality and delayed hatching of zebrafish larvae in a dose-dependent manner. Cytisine did not affect mortality in a wide range of concentrations, and hatching delay was observed only at the highest concentrations, above 2 mM. Administering compounds together partially reduced the adverse teratogenic effect induced by nicotine alone. The protective effect of cytisine against the nicotine effect, observed in zebrafish, will contribute to future studies or treatments related to nicotine addiction or prenatal nicotine exposure in humans.

Selected Applications of Chitosan Composites.

Chitosan is one of the emerging materials for various applications. The most intensive studies have focused on its use as a biomaterial and for biomedical, cosmetic, and packaging systems. The research on biodegradable food packaging systems over conventional non-biodegradable packaging systems has gained much importance in the last decade. The deacetylation of chitin, a polysaccharide mainly obtained from crustaceans and shrimp shells, yields chitosan. The deacetylation process of chitin leads to the generation of primary amino groups. The functional activity of chitosan is generally owed to this amino group, which imparts inherent antioxidant and antimicrobial activity to the chitosan. Further, since chitosan is a naturally derived polymer, it is biodegradable and safe for human consumption. Food-focused researchers are exploiting the properties of chitosan to develop biodegradable food packaging systems. However, the properties of packaging systems using chitosan can be improved by adding different additives or blending chitosan with other polymers. In this review, we report on the different properties of chitosan that make it suitable for food packaging applications, various methods to develop chitosan-based packaging films, and finally, the applications of chitosan in developing multifunctional food packaging materials. Here we present a short overview of the chitosan-based nanocomposites, beginning with principal properties, selected preparation techniques, and finally, selected current research.

Insight into structural and chemical profile / composition of powdered enamel and dentine in different types of permanent human teeth.

Research on the structure and chemical composition of dental tissues allows for the optimisation of materials used in the treatment and care of teeth. Understanding pathological processes occurring in dental tissues and their reactions to various substances, including dental materials, are crucial for the development of new dental technologies. The aim of the study was to check the similarities in the chemical and morphological structure of enamel and dentine powders in various groups of permanent teeth, as well as differential chemical analysis for both grinded tissues tested. The extracted non-carious and non-pathological human permanent teeth were divided into four groups: incisors, canines, premolars and molars. Each tooth was sectioned to thick slices. Enamel and dentine were mechanically separated and ground in an agate mortar and pestle. FT-Raman and FTIR spectroscopy methods were used for the analysis of biological tissues. SEM method was applied to visualise hard dental tissues structures present on the surface and within the particles. The morphological structures were the same within the analysed tissues and did not depend on the analysed group of teeth. A comparison of the mineral-to-organic ratios of enamel and dentine in each tooth group showed that the bands related to PO43- were clearly higher in content for enamel than for dentine. Higher absorbance measured at the region of 2800-3700 cm-1 and at 1500-1800 cm-1 for dentine as compared to enamel samples were indicative of a higher content of organic structures. The highest contribution of phosphates was in canine enamel samples.The studies showed that the carbonate-to-phosphate ratio was higher for dentine (0.20 - 0.48) compared to the values obtained for enamel (0.13 - 0.22), however, minor differences were found in each group of enamel or dentine samples. The lack of significant differences between the enamel and dentine powders of incisors, canines, premolars and molars may prove that each extracted tooth, regardless of the tooth group, is an excellent substrate for their substitution.

Root Canal Infection and Its Impact on the Oral Cavity Microenvironment in the Context of Immune System Disorders in Selected Diseases: A Narrative Review.

The oral cavity has a specific microenvironment, and structures such as teeth are constantly exposed to chemical and biological factors. Although the structure of the teeth is permanent, due to exposure of the pulp and root canal system, trauma can have severe consequences and cause the development of local inflammation caused by external and opportunistic pathogens. Long-term inflammation can affect not only the local pulp and periodontal tissues but also the functioning of the immune system, which can trigger a systemic reaction. This literature review presents the current knowledge on root canal infections and their impact on the oral microenvironment in the context of immune system disorders in selected diseases. The result of the analysis of the literature is the statement that periodontal-disease-caused inflammation in the oral cavity may affect the development and progression of autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome, as well as affecting the faster progression of conditions in which inflammation occurs such as, among others, chronic kidney disease or inflammatory bowel disease.

Occurrence of non-carious enamelopathies in the children living in the vicinity of the Coal Mine Bogdanka.

The main aim of this study was to examine the incidence of enamelopathies among children from the Bogdanka region. Altogether, there were examined 273 people (aged 7-14) who live in the neigbourhood of the mainfield (group I). The controlled group consisted of 188 children of the same age who lived in the areas free of chemical pollution (group II). The appearing of distempers in the structure of the enamel was marked among 62 people, the majority of whom were the boys (39), girls--23. When it comes to the placement of these changes, they were mostly located in the labial surface of incisors of the maxilla, less frequently in the canines and premolar teeth. On the other hand, just particular changes were spotted in the mandibula. The changes mentioned above were characterized by the white turbidity, were well-limited and did not show the symmetry. In none of the cases the decay appeared as an additional factor. The clinical researches were enriched by the survey research aiming to estimate the existence and the level of discomfort among children suffering from enamelopathies.