Dynamic changes in lung water density and volume following supine body positioning.

PURPOSE: Measure the changes in relative lung water density (rLWD), lung volume, and total lung water content as a function of time after supine body positioning. METHODS: An efficient ultrashort-TE pulse sequence with a yarnball k-space trajectory was used to measure water density-weighted lung images for 25 min following supine body positioning (free breathing, 74-s acquisitions, 3D images at functional residual capacity, 18 time points) in 9 healthy volunteers. Global and regional (10 chest-to-back positions) rLWD, lung volume, and total lung water volume were measured in all subjects at all time points. Volume changes were validated with a nitrogen washout study in 3 participants. RESULTS: Global rLWD increased significantly (p = 0.001) from 31.8 ± 5.5% to 34.8 ± 6.8%, while lung volumes decreased significantly (p < 0.001) from 2390 ± 620 mL to 2130 ± 630 mL over the same 25-min interval. Total lung water volume decreased slightly from 730 ± 125 mL to 706 ± 126 mL (p = 0.028). There was a significant chest-to-back gradient in rLWD (20.7 ± 4.6% to 39.9 ± 6.1%) at all time points with absolute increases of 1.8 ± 1.2% at the chest and 5.4 ± 1.9% at the back. Nitrogen washout studies yielded a similar reduction in lung volume (12.5 ± 0.9%) and time course following supine positioning. CONCLUSION: Lung volumes during tidal breathing decrease significantly over tens of minutes following supine body positioning, with corresponding increases in lung water density (9.2 ± 4.4% relative increase). The total volume of lung water is slightly reduced over this interval (3.3 ± 4.0% relative change). Evaluation of rLWD should take time after supine positioning, and more generally, all sources of lung volume changes should be taken into consideration to avoid significant bias.

Diffusion time dependency along the human corpus callosum and exploration of age and sex differences as assessed by oscillating gradient spin-echo diffusion tensor imaging.

Conventional diffusion imaging uses pulsed gradient spin echo (PGSE) waveforms with diffusion times of tens of milliseconds (ms) to infer differences of white matter microstructure. The combined use of these long diffusion times with short diffusion times (<10 â€‹ms) enabled by oscillating gradient spin echo (OGSE) waveforms can enable more sensitivity to changes of restrictive boundaries on the scale of white matter microstructure (e.g. membranes reflecting the axon diameters). Here, PGSE and OGSE images were acquired at 4.7 â€‹T from 20 healthy volunteers aged 20-73 years (10 males). Mean, radial, and axial diffusivity, as well as fractional anisotropy were calculated in the genu, body and splenium of the corpus callosum (CC). Monte Carlo simulations were also conducted to examine the relationship of intra- and extra-axonal radial diffusivity with diffusion time over a range of axon diameters and distributions. The results showed elevated diffusivities with OGSE relative to PGSE in the genu and splenium (but not the body) in both males and females, but the OGSE-PGSE difference was greater in the genu for males. Females showed positive correlations of OGSE-PGSE diffusivity difference with age across the CC, whereas there were no such age correlations in males. Simulations of radial diffusion demonstrated that for axon sizes in human brain both OGSE and PGSE diffusivities were dominated by extra-axonal water, but the OGSE-PGSE difference nonetheless increased with area-weighted outer-axon diameter. Therefore, the lack of OGSE-PGSE difference in the body is not entirely consistent with literature that suggests it is composed predominantly of axons with large diameter. The greater OGSE-PGSE difference in the genu of males could reflect larger axon diameters than females. The OGSE-PGSE difference correlation with age in females could reflect loss of smaller axons at older ages. The use of OGSE with short diffusion times to sample the microstructural scale of restriction implies regional differences of axon diameters along the corpus callosum with preliminary results suggesting a dependence on age and sex.

Radiofrequency excitation-related 23 Na MRI signal loss in skeletal muscle, cartilage, and skin.

PURPOSE: To assess the sodium MRI signal loss resulting from typically used RF excitation pulses in human skeletal muscle, patellar cartilage, and skin. METHODS: A double flip-angle experiment was performed 3 times on the knees of 5 healthy volunteers with prescribed ω1 = γB1 of 1.67 kHz, 0.333 kHz, and 0.167 kHz. This was done to search for ω1 -dependent increased rates of sodium-23 central resonance flipping known to result from residual quadrupole splitting (ωQ ), as this flip-angle effect is associated with signal loss. This study facilitated in vivo regression of Gaussian-distributed residual quadrupole splitting SD (ωQ(SD) ) as well as T2fast and T2slow . Signal loss predicted from simulation was then compared with images acquired using 90° RF pulse lengths of 0.5 ms, 0.25 ms, and 0.15 ms. RESULTS: Sodium-23 central resonance flipping was significantly greater than prescribed (44% cartilage, 23% skin, 9% muscle) using ω1 = 0.167 kHz, but only 4% cartilage, 5% skin, and 2% muscle using ω1 = 1.67 kHz. Regression yielded ωQ(SD) = 420 ± 50 Hz for cartilage but no significant ωQ(SD) for skin or muscle. This points to rapid biexponential relaxation as the cause of the flip-angle effect for skin/muscle. The T2fast(60%) /T2slow(40%) values were 1.6 ± 0.8 ms/16.1 ± 2.5 ms for muscle, 2.7 ± 0.9 ms/18.4 ± 2.5 ms for cartilage, and 0.4 ± 0.1 ms/9.3 ± 1.7 ms for skin. Simulation predicted signal loss of 6% ± 3%, 3% ± 1%, and 2% ± 1% for muscle, 16% ± 3%, 6% ± 1%, and 3% ± 1% for cartilage, and 26% ± 7%, 15% ± 4%, and 10% ± 3% for skin when using 90° RF pulse lengths of 0.5 ms, 0.25 ms, and 0.15 ms, matching experiment. CONCLUSION: High-power (short) RF pulses are necessary to reduce excitation-related signal loss, particularly for sodium-23 imaging of cartilage and skin.