[STATE OF NONALLERGIC HYPERSENSITIVITY UNDER THE EXPOSURE TO ADVERSE ENVIRONMENTAL FACTORS].

Now a number of allergopatologies is shown to be formed as non-allergic hypersensitivity. Research of this state is especially important when studying a question of formation of a condition of a chemical sensitization in response to the contact of a human body with chemical pollutants. For diagnostics of reactions of non-allergic hypersensitivity the test was developed for an assessment of activation of basophiles by specific allergens (the test of activation of basophiles by method of a flowing cytometry Flow Cast® (BD FACSCalibur)). It is possible to judge by activation on an expression of some receptors on a surface of these cages (CD63). Examination of a group of persons, having continuous contact with means of household chemicals was performed. In 54.5% of the examined persons the percent of CD63-of nonspecific activated cages was revealed to be at the level of 16-65% that gives the grounds to assume existence of a condition of not allergic hypersensitivity. Thus, the test of activation of basophiles by method of a flowing cytometry can be used for a differentiation of an IgE-dependent allergy from a condition of non-allergic hypersensitivity.

[Experimental data for evaluation of biological effect during exposure to chemical and biological factors]. / Eksperimentalnye dannye po otsenke biologicheskogo éffecta pri vozdeistvii khimicheskogo i biologicheskogo faktorov.

Joint effects of lysin, eprin and formaldehyde in acute and subacute experiments were investigated. As shown, this effect can be evaluated as a partial summing with coefficient 1.84.

[Protein spectra and phospholipid composition of cholinoreceptor-enriched membranes from rat skeletal muscles after sensitization]. / Belkovye spektry i fosfolipidnyi sostav membran, obogashchennykh kholinoretseptorami, iz skeletnykh myshts krys v usloviiakh sensibilizatsii.

The effect of neisserial sensitization on the protein spectra and phospholipid composition of acetyscholine receptor-rich membrane fragments was investigated in rat skeletal muscles. The sensitization led to substantial changes in the protein spectrum of the cell membranes of the skeletal muscles with high receptor activity in respect to choline. The phospholipid composition of the membranes was found to experience profound transformations. A considerable increase was noted in the content of sphingomye lin and phosphatidylcholine together with a marked drop in the content of phosphatidylethanolamine, phosphatidylinosite and phosphatidylserine.