Effects of physical activity on the levels of remnant cholesterol: A population-based study.

Physical activity (PA) has the potential to bring about favourable changes in plasma lipid profile. However, the relationship between PA and remnant cholesterol (RC) remains unclear. We aimed to study the link between PA and RC using the database of the 2007-2020 National Health and Nutrition Examination Survey (NHANES). PA was categorized based on Physical Activity Guidelines for Americans. A multivariate linear regression model was used to determine the correlations between PA and RC. The study involved a total of 18,396 participants and revealed that individuals whose PA met the guidelines by engaging in moderate-intensity PA at least 150 min per week had lower body mass index and showed decreased levels of triglyceride, TC, and haemoglobin A1c compared to those who were physically inactive, exercising <150 min per week. Participants whose intensity of PA meets PA guidelines had a lower level of RC than those who did not met PA guidelines (ß = -1.3, 95% confidence interval [CI]: -1.9 to -0.7, p < 0.001), even after adjusting for confounders. During subgroup analysis, we observed that race (pinteraction = 0.0089) emerged as a significant factor of interaction.

Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer.

USP14 deubiquitinates ERα to maintain its stability in ECEndometrial cancer (EC) is one of the common gynecological malignancies of which the incidence has been rising for decades. It is considered that continuously unopposed estrogen exposure is the main risk factor for EC initiation. Thus, exploring the modulation of estrogen/estrogen receptor α (ERα) signaling pathway in EC would be helpful to well understand the mechanism of EC development and find the potential target for EC therapy. Ubiquitin-specific peptidase 14 (USP14), a member of the proteasome-associated deubiquitinating enzyme family, plays a crucial role in a series of tumors. However, the function of USP14 in EC is still elusive. Here, our results have demonstrated that USP14 is highly expressed in EC tissues compared with that in normal endometrial tissues, and higher expression of USP14 is positively correlated with poor prognosis. Moreover, USP14 maintains ERα stability through its deubiquitination activity. Our results further demonstrate that USP14 depletion decreases the expression of ERα-regulated genes in EC-derived cell lines. Moreover, knockdown of USP14 or USP14-specific inhibitor treatment significantly suppresses cell growth and migration in EC cell lines or in mice. We further provide the evidence to show that the effect of USP14 on EC cell growth, if not all, at least is partially related to ERα pathway. Our study provides new sights for USP14 to be a potential therapeutic target for the treatment of EC, especially for EC patients with fertility preservation needs.

The serum soluble ASGR1 concentration is elevated in patients with coronary artery disease and is associated with inflammatory markers.

BACKGROUND AND AIMS: Current research has suggested that asialoglycoprotein receptor 1 (ASGR1) is involved in cholesterol metabolism and is also related to systemic inflammation. This study aimed to assess the correlation between the serum soluble ASGR1 (sASGR1) concentration and inflammatory marker levels. Moreover, the second objective of the study was to assess the association between sASGR1 levels and the presence of coronary artery disease (CAD). METHODS: The study subjects included 160 patients who underwent coronary angiography. Ninety patients were diagnosed with CAD, while seventy age- and sex-matched non-CAD patients served as controls. We measured the serum sASGR1 levels using an ELISA kit after collecting clinical baseline characteristics. RESULTS: Patients with CAD had higher serum sASGR1 levels than non-CAD patients did (P < 0.0001). sASGR1 was independently correlated with the risk of CAD after adjusting for confounding variables (OR = 1.522, P = 0.012). The receiver operating characteristic (ROC) curve showed that sASGR1 had a larger area under the curve (AUC) than did the conventional biomarkers apolipoprotein B (APO-B) and low-density lipoprotein cholesterol (LDL-C). In addition, multivariate linear regression models revealed that sASGR1 is independently and positively correlated with high-sensitivity C-reactive protein (CRP) (ß = 0.86, P < 0.001) and WBC (ß = 0.13, P = 0.004) counts even after adjusting for lipid parameters. According to our subgroup analysis, this relationship existed only for CAD patients. CONCLUSION: Our research demonstrated the link between CAD and sASGR1 levels, suggesting that sASGR1 may be an independent risk factor for CAD. In addition, this study provides a reference for revealing the potential role of sASGR1 in the inflammation of atherosclerosis.

Systematic investigation of metal dopants and mechanism for the SnO2 electron transport layer in perovskite solar cells.

SnO2, the most promising alternative to TiO2 as the electron transport layer (ETL), has attracted great attention for perovskite solar cells (PSCs) due to its high bulk electron mobility, good band energy at the ETL/perovskite interface, and high chemical stability. To enable more efficient carrier transfer and extraction, elemental doping with different metal cations has been studied in SnO2 ETLs. However, the systematic investigation of the doping mechanism lag far behind their efficiency promotion. In this paper, elements of the same main group (Li, Na, K) and period (K, Ca, Ga) have been selected for doping in SnO2. The results showed that among the properties of the dopants, the electronegativity has the greatest influence. The smaller the electronegativity of the doping species, the more conducive it is to carrier transmission and separation. The corresponding mechanism was proposed and discussed. At last, an efficiency of 20.92% of PSCs based on SnO2-K was achieved. In addition, the doped SnO2 is more beneficial for the growth of perovskite crystals, thus reducing grain boundaries and enhancing the stability of the device.

Correlation between serum soluble ASGR1 concentration and low-density lipoprotein cholesterol levels: a cross-sectional study.

BACKGROUND: Recent studies have shown that loss-of-function mutations in hepatic asialoglycoprotein receptor 1 (ASGR1) are associated with low levels of circulating cholesterol and a reduced risk of coronary artery disease (CAD). In contrast to ASGR1 on the hepatocyte membrane, serum soluble ASGR1 (sASGR1) is a secreted form that has been detected in circulation. However, the functions of serum sASGR1 are unclear. This study aims to investigate the relationship between human serum sASGR1 concentration and low-density lipoprotein cholesterol (LDL-C) levels. METHODS: In a cohort of 134 participants who underwent coronary angiography examination, basic information was recorded, and blood samples were collected for biochemical testing. The serum sASGR1 concentration was determined by ELISA kits. The relationship between sASGR1 concentration and LDL-C levels was examined using linear regression models and interaction tests. Univariate and multivariate analyses were used to identify clinical variables that affect sASGR1 levels. RESULTS: After adjusting for potential confounders such as age, sex, BMI, and statin use, the serum sASGR1 concentration was positively correlated with LDL-C levels (ß = 0.093, 95% CI: 0.04 to 0.14, P < 0.001). Subgroup analysis and interaction tests showed that the effect of serum sASGR1 concentration on LDL-C levels was significantly influenced by hypertension status (P for interaction = 0.0067). The results of a multivariate linear regression analysis incorporating age, serum TG, LDL-C, nonesterified fatty acid (NEFA), white blood cell counts (WBCC), and fibrinogen revealed that LDL-C (ß = 1.005, 95% CI: 0.35 to 1.66, P = 0.003) and WBCC (ß = 0.787, 95% CI: 0.41 to 1.16, P < 0.0001) were independent influencing factors for serum sASGR1 levels. CONCLUSIONS: The serum sASGR1 concentration was positively correlated with LDL-C levels. In addition, hypertension status significantly affected the effect of serum sASGR1 on LDL-C levels. This study provides some research ideas for clinical doctors and researchers, as well as some references for additional research on serum sASGR1.

Enhancing N uptake and reducing N pollution via green, sustainable N fixation-release model.

The overuse of N fertilizers has caused serious environmental problems (e.g., soil acidification, excessive N2O in the air, and groundwater contamination) and poses a serious threat to human health. Improving N fertilizer utilization efficiency and plant uptake is an alternative for N fertilizers overuses. Enterobacter cloacae is an opportunistic pathogen, also used as plant growth-promoting rhizobacteria (PGPR), has been widely presented in the fields of bioremediation and bioprotection. Here we developed a new N fixation-release model by combining biochar with E. cloacae. The efficiency of the model was evaluated using a greenhouse pot experiment with maize (Zea mays L.) as the test crop. The results showed that biochar combined with E. cloacae significantly increased the N content. The application of biochar combined with E. cloacae increased total N in soil by 33% compared with that of N fertilizers application. The N-uptake and utilization efficiency (NUE) in plant was increased 17.03% and 14.18%, respectively. The activities of urease, dehydrogenase and fluorescein diacetate hydrolase (FDA) was improved, the catalase (CAT) activity decreased. Analysis of the microbial community diversity revealed the abundance of Proteobacteria, Actinobacteria, Firmicutes, and Gemmatimonadetes were significantly improved. The mechanism under the model is that E. cloacae acted as N-fixation by capturing N2 from air. Biochar served as carrier, supporting better living environment for E. cloacae, also as adsorbent adsorbing N from fertilizer and from fixed N by E. cloacae, the adsorption in turn slower the N release. Altogether, the model promotes N utilization by plants, improves the soil environment, and reduces N pollution.

Uric acid and blood pressure in NHANES dated from 2009 to 2018: A cross-sectional research.

BACKGROUND AND AIM: This study aimed to explore the association between uric acid (UA) and blood pressure (BP), included systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP). METHODS AND RESULTS: A cross-sectional study with 22,478 individuals aged from 12 to 80 years (11,443 males and 11,035 females) from the National Health and Nutrition Examination Survey (NHANES) was performed. Multiple linear regression analysis was applied to explore the relationship between UA and BP, Stratified analysis and interaction were performed based on gender, race, age, body mass index (BMI), and alcohol consumption. Significantly positively associations were presented in SBP(ß, 0.84 [95% CI, 0.67, 1.00]), DBP(ß, 0.23 [95% CI, 0.11, 0.36]), and MAP (ß, 0.43 [95% CI, 0.31, 0.55]). The associations were much more stronger between UA and SBP in females (ß, 1.04 [95% CI, 0.78, 1.30], p for interaction 0.0003), black group (ß, 1.17 [95% CI, 0.77, 1.56], p for interaction 0.0296), age (≥45) group (ß, 1.03 [95% CI, 0.68, 1.39], p for interaction <0.0001) and drinking group (ß, 0.98 [95% CI, 0.75, 1.21], p for interaction <0.0001). The significant interactions were found between UA and DBP in gender and alcohol consumption (all p for interaction <0.05). In terms of MAP, the significant interactions were found in race, age, and alcohol consumption (all p for interaction <0.05). CONCLUSIONS: A significantly positively association was found between UA and BP, including SBP, DBP, and MAP.

Enhanced laccase production by mutagenized Myrothecium verrucaria using corn stover as a carbon source and its potential in the degradation of 2-chlorophen.

Chlorophenols are widely used in industry and are known environmental pollutants. The degradation of chlorophenols is important for environmental remediation. In this study, we evaluated the biodegradation of 2-chlorophenol using crude laccase produced by Myrothecium verrucaria. Atmospheric and room temperature plasma technology was used to increase laccase production. The culture conditions of the M-6 mutant were optimized. Our results showed that corn stover could replace glucose as a carbon source and promote laccase production. The maximum laccase activity of 30.08 U/mL was achieved after optimization, which was a 19.04-fold increase. The biodegradation rate of 2-chlorophenol using crude laccase was 97.13%, a positive correlation was determined between laccase activity and degradation rate. The toxicity of 2-CP was substantially reduced after degradation by laccase solution. Our findings show the feasibility of the use of corn stover in laccase production by M. verrucaria mutant and the subsequent biodegradation of 2-chlorophenol using crude laccase.

One-Step Synthesis of Nitrogen-Doped Porous Biochar Based on N-Doping Co-Activation Method and Its Application in Water Pollutants Control.

In this work, birch bark (BB) was used for the first time to prepare porous biochars via different one-step methods including direct activation (BBB) and N-doping co-activation (N-BBB). The specific surface area and total pore volume of BBB and N-BBB were 2502.3 and 2292.7 m2/g, and 1.1389 and 1.0356 cm3/g, respectively. When removing synthetic methyl orange (MO) dye and heavy metal Cr6+, both BBB and N-BBB showed excellent treatment ability. The maximum adsorption capacities of BBB and N-BBB were 836.9 and 858.3 mg/g for MO, and 141.1 and 169.1 mg/g for Cr6+, respectively, which were higher than most previously reported biochar adsorbents. The probable adsorption mechanisms, including pore filling, π-π interaction, H-bond interaction, and electrostatic attraction, supported the biochars' demonstrated high performance. In addition, after five recycles, the removal rates remained above 80%, which showed the high stability of the biochars. This work verified the feasibility of the one-step N-doping co-activation method to prepare high-performance biochars, and two kinds of biochars with excellent performance (BBB and N-BBB) were prepared. More importantly, this method provides new directions and ideas for the development and utilization of other biomasses.

Influence of Microbial Treatment on the Preparation of Porous Biochar with Stepped-Up Performance and Its Application in Organic Pollutants Control.

In this study, Irisensata Thunb grass (ITG) was used as a biomass carbon resource to prepare biochars for the first time. After microbial treatment, the obtained microbial-treated ITG (MITG) was activated by using a mixed base as an activator for preparation of biochar (MITGB). The specific surface area and total pore volume of MITGB were 3036.4 m2/g and 1.5252 cm3/g, which were higher than those of biochar prepared without microbial treatment (ITGB, 2930.0 m2/g and 1.5062 cm3/g). Besides, the physicochemical properties of MITGB and ITGB were also quite different including micro morphology, surface chemistry, functional groups, etc. In the experiment of removing organic pollutants with synthetic dye RhB and antibiotic TH as the models, MITGB showed excellent treatment ability. The maximum adsorption capacities of MITGB for RhB and TH were 1354.2 and 1462.6 mg/g, which were higher than most of the biochars. In addition, after five cycles of recycling, the adsorption capacities of the organic pollutant models can still be maintained at more than 80%, which showed high stability. This work verified the feasibility of microbial treatment to further improve the performance of biochar and provided a new idea and direction for exploring other biochars.

Magnetic Luffa-Leaf-Derived Hierarchical Porous Biochar for Efficient Removal of Rhodamine B and Tetracycline Hydrochloride.

Luffa leaf (LL) is an agricultural waste produced by loofah. In this work, LL was used as biomass carbon source for biochars for the first time. After carbonization, activation, and chemical co-precipitation treatments, a magnetic lignocellulose-derived hierarchical porous biochar was obtained. The specific surface area and total pore volume were 2565.4 m2/g and 1.4643 cm3/g, and the surface was rich in carbon and oxygen functional groups. The synthetic dye rhodamine B (RhB) and the antibiotic tetracycline hydrochloride (TH) were selected as organic pollutant models to explore the ability to remove organic pollutants, and the results showed good adsorption performances. The maximum adsorption capacities were 1701.7 mg/g for RhB and 1755.9 mg/g for TH, which were higher than most carbon-based adsorbents. After 10 cycles of use, the removal efficiencies were still maintained at more than 70%, showing good stability. This work not only verified the feasibility of lignocellulose LL as a carbon source to prepare biochar but also prepared a magnetic hierarchical porous adsorbent with good performances that can better treat RhB and TH, which provided a new idea and direction for the efficient removal of organic pollutants in water.

One-Step Carbonization Synthesis of Magnetic Biochar with 3D Network Structure and Its Application in Organic Pollutant Control.

In this study, a magnetic biochar with a unique 3D network structure was synthesized by using a simple and controllable method. In brief, the microbial filamentous fungus Trichoderma reesei was used as a template, and Fe3+ was added to the culture process, which resulted in uniform recombination through the bio-assembly property of fungal hyphae. Finally, magnetic biochar (BMFH/Fe3O4) was synthesized by controlling different heating conditions in a high temperature process. The adsorption and Fenton-like catalytic performance of BMFH/Fe3O4 were investigated by using the synthetic dye malachite green (MG) and the antibiotic tetracycline hydrochloride (TH) as organic pollutant models. The results showed that the adsorption capacity of BMFH/Fe3O4 for MG and TH was 158.2 and 171.26 mg/g, respectively, which was higher than that of most biochar adsorbents, and the Fenton-like catalytic degradation effect of organic pollutants was also better than that of most catalysts. This study provides a magnetic biochar with excellent performance, but more importantly, the method used can be effective in further improving the performance of biochar for better control of organic pollutants.

Cow Dung-Based Biochar Materials Prepared via Mixed Base and Its Application in the Removal of Organic Pollutants.

Cow dung (CD) is a waste product of livestock production. Improper disposal of a large amount of CD will cause environmental pollution. In this work, three biochar materials based on CD (BMCD) were prepared by using three types of base, including KOH, NaOH, and mixed base (MB, a mixture of equal mass NaOH and KOH) as activators to investigate the different physicochemical properties of BMCDs (BMCD-K, BMCD-Na, and BMCD-MB). The objective was to verify the effectiveness of MB activation in the preparation of biochar materials. The results show that MB has an effect on the structural characteristics of BMCDs. In particular, the surface area and total pore volume, the specific surface area, and the total pore volume of BMCD-MB (4081.1 m2 g-1 and 3.0118 cm3 g-1) are significantly larger than those of BMCD-K (1784.6 m2 g-1 and 1.1142 cm3 g-1) and BMCD-Na (1446.1 m2 g-1 and 1.0788 cm3 g-1). While synthetic dye rhodamine B (RhB) and antibiotic tetracycline hydrochloride (TH) were selected as organic pollutant models to explore the adsorption performances, the maximum adsorption capacities of BMCD-K, BMCD-NA and BMCD-MB were 951, 770, and 1241 mg g-1 for RhB, 975, 1051, and 1105 mg g-1 for TH, respectively, which were higher than those of most adsorbents. This study demonstrated that MB can be used as an effective activator for the preparation of biochar materials with enhanced performance.

Evaluation of Preparation and Detoxification of Hemicellulose Hydrolysate for Improved Xylitol Production from Quinoa Straw.

Quinoa straw is rich in hemicellulose, and it could be hydrolyzed into xylose. It is a promising energy resource alternative that acts as a potential low-cost material for producing xylitol. In this study, quinoa straw was used as a substrate subjected to the hydrolysis of dilute sulfuric acid solution. Based on the production of xylose and inhibitors during hydrolysis, the optimal conditions for the hydrolysis of hemicellulose in quinoa straw were determined. Detoxification was performed via activated carbon adsorption. The optimal detoxification conditions were determined on the basis of major inhibitor concentrations in the hydrolysate. When the addition of activated carbon was 3% at 30 °C for 40 min, the removal of formic acid, acetic acid, furfural, and 5-HMF could reach 66.52%, 64.54%, 88.31%, and 89.44%, respectively. In addition to activated carbon adsorption, vacuum evaporation was further conducted to perform two-step detoxification. Subsequently, the detoxified hydrolysate was used for xylitol fermentation. The yield of xylitol reached 0.50 g/g after 96 h of fermentation by Candida tropicalis (CICC 1779). It is 1.2-fold higher than that obtained through the sole vacuum evaporation method. This study validated the feasibility of xylitol production from quinoa straw via a biorefinery process.

Unveiling the Effect of Solvents on Crystallization and Morphology of 2D Perovskite in Solvent-Assisted Method.

Controlling the crystallographic orientations of 2D perovskite is regarded as an effective way to improve the efficiency of PSCs based on 2D perovskite. In this paper, five different assistant solvents were selected to unveil the effect of solvents on crystallization and morphology of 2D perovskite in a solvent-assisted method. Results demonstrated that the effect of Lewis basicity on the crystallization process was the most important factor for preparing 2D perovskite. The stability of the intermediate, reacted between the solvent and the Pb2+, determined the quality of 2D film. The stronger the Lewis basicity was, the more obvious the accurate control effect on the top-down crystallization process of 2D perovskite would be. This could enhance the crystallographic orientation of 2D perovskite. The effect of Lewis basicity played a more important role than other properties of the solvent, such as boiling point and polarity.

ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.

Absent, small or homeotic 2-like protein (ASH2L) is a core component of a multimeric histone methyltransferase complex that is involved in the maintenance of active transcription, participating in several cancers, however the biological function and molecular mechanism of ASH2L in endometrial cancer (ECa) are largely unknown. Endometrial cancer is a common malignant tumor in women and the incidence of this cancer is on the rise. Estrogen-ERα signaling, as an oncogenic pathway, plays a crucial role in endometrial carcinogenesis. Therefore, further exploration of the molecular mechanisms around ERα-mediated gene transcription in ECa would be helpful to the understanding of tumor development and to finding a new therapeutic target for ECa. Here, our study demonstrated that ASH2L was highly expressed in ECa samples, and higher expression of ASH2L was positively correlated with a poor prognosis. Moreover, we identified that ASH2L associated with ERα and that knockdown of ASH2L resulted in decreased expression of a subset of the estrogen-induced target genes, including paired box 2 (PAX2), an oncogenic gene in ECa. ASH2L was recruited to cis-regulatory elements in PAX2, thereby altering histone H3K4me3 and H3K27me3 levels, to enhance ERα-mediated transactivation. Finally, depletion of ASH2L suppressed endometrial cancer cell proliferation and migration. Our findings suggest that ASH2L participates in the promotion of ECa progression, if not totally at least partially, via upregulation of PAX2 transcription.

The bHLH gene family and its response to saline stress in Jilin ginseng, Panax ginseng C.A. Meyer.

Basic helix-loop-helix (bHLH) gene family is a gene family of transcription factors that plays essential roles in plant growth and development, secondary metabolism and response to biotic and abiotic stresses. Therefore, a comprehensive knowledge of the bHLH gene family is paramount to understand the molecular mechanisms underlying these processes and develop advanced technologies to manipulate the processes efficiently. Ginseng, Panax ginseng C.A. Meyer, is a well-known medicinal herb; however, little is known  about the bHLH genes (PgbHLH) in the species. Here, we identified 137 PgbHLH genes from Jilin ginseng cultivar, Damaya, widely cultivated in Jilin, China, of which 50 are newly identified by pan-genome analysis. These 137 PgbHLH genes were phylogenetically classified into 26 subfamilies, suggesting their sequence diversification. They are alternatively spliced into 366 transcripts in a 4-year-old plant and involved in 11 functional subcategories of the gene ontology, indicating their functional differentiation in ginseng. The expressions of the PgbHLH genes dramatically vary spatio-temporally and across 42 genotypes, but they are still somehow functionally correlated. Moreover, the PgbHLH gene family, at least some of its genes, is shown to have roles in plant response to the abiotic stress of saline. These results provide a new insight into the evolution and functional differentiation of the bHLH gene family in plants, new bHLH genes to the PgbHLH gene family, and saline stress-responsive genes for genetic improvement in ginseng and other plant species.

Innovative hydrolysis of corn stover biowaste by modified magnetite laccase immobilized nanoparticles.

Intensive studies have been performed on the improvement of bioethanol production by transformation of lignocellulose biomass. In this study, the digestibility of corn stover was dramatically improved by using laccase immobilized on Cu2+ modified recyclable magnetite nanoparticles, Fe3O4-NH2. After digestion, the laccase was efficiently separated from slurry. The degradation rate of lignin reached 40.76%, and the subsequent cellulose conversion rate 38.37% for 72 h at 35 °C with cellulase at 50 U g-1 of corn stover. Compared to those of free and inactivated mode, the immobilized laccase pre-treatment increased subsequent cellulose conversion rates by 23.98% and 23.34%, respectively. Moreover, the reusability of immobilized laccase activity remained 50% after 6 cycles. The storage and thermal stability of the fixed laccase enhanced by 70% and 24.1% compared to those of free laccase at 65 °C, pH 4.5, respectively. At pH 10.5, it exhibited 16.3% more activities than its free mode at 35 °C. Our study provides a new avenue for improving the production of bioethanol with immobilized laccase for delignification using corn stover as the starting material.

Association between admission hemoglobin level and prognosis in sepsis patients based on a critical care database.

This study aimed to explore the association between admission hemoglobin level and clinical outcomes in sepsis based on Medical Information Mart for Intensive Care IV(MIMIC-IV) database. It was a retrospective study. Sepsis patients in the database were included. Data extraction from MIMIC-IV was performed by PostgreSQL 9.6 software. Three different models including crude model (adjusted for none), model I (adjusted for gender and age) and model II (adjusted for all potential cofounders) were constructed. A generalized liner model and a smooth fitting curve for indicating the relationship between hemoglobin level and 30-day mortality were performed. 6249 septic patients with a 30.18% of 30-day mortality were included. With 1 g/dl increment in hemoglobin level, the values of odds ratio (OR) in crude model, model I and model II were 0.96 (95% confidential interval (CI) 0.94-0.99, P = 0.0023), 0.96 (95%CI 0.93-0.98, P = 0.0010) and 0.87 (95%CI 0.79-0.95, P = 0.0020), respectively. The smooth fitting curve indicated a non-linear relationship and the turning point was 7.2 g/dl. Compared the hemoglobin group < 7.2 g/dl, the risk of 30-day mortality significantly decreased by 32% in the hemoglobin group ≥ 7.2 g/dl (OR = 0.68, 95%CI 0.51-0.93, P = 0.0142). The non-linear relationship between admission hemoglobin level and 30-day mortality in sepsis was found. Hemoglobin supplementation might be beneficial for septic patients with hemoglobin level < 7.2 g/dl.

Establishment and Validation of an Early Predictive Model for Severe Acute Pancreatitis.

Objective: The purpose of this study is to establishment and validation of an early predictive model for severe acute pancreatitis (SAP). Methods: From January 2015 to August 2022, 2986 AP patients admitted to Changsha Central Hospital were enrolled in this study. They were randomly divided into a modeling group (n = 2112) and a validation group (n = 874). In the modeling group, identify risk factors through logistic regression models and draw column charts. Use internal validation method to verify the accuracy of column chart prediction. Apply calibration curves to evaluate the consistency between nomograms and ideal observations. Draw a DCA curve to evaluate the net benefits of the prediction model. Results: Nine variables including respiratory rate, heart rate, WBC, PDW, PT, SCR, AMY, CK, and TG are the risk factors for SAP. The column chart risk prediction model which was constructed based on these 9 independent factors has high prediction accuracy (modeling group AUC = 0.788, validation group AUC = 7.789). The calibration curve analysis shows that the prediction probabilities of the modeling and validation groups are consistent with the observation probabilities. By drawing a DCA curve, it shows that the model has a wide threshold range (0.01-0.88). Conclusion: The study developed an intuitive nomogram containing readily available laboratory parameters to predict the incidence rate of SAP.