[Effects of sevoflurane on the migration of vascular endothelial cells].

PURPOSE: To evaluate the effects of sevoflurane on cell migration and expression of VE-cadherin mRNA in human umbilical vein endothelial cells(HUVECs). METHODS: HUVECs were allocated to 4 groups randomly (each group contained 4 culture dishes with a diameter of 10 cm), and were exposed to 2% sevoflurane (refer to 1.6 MAC) respectively for 0 h (control group which exposed to the same environment as the other 3 groups but without sevoflurane), 0.5 h, 1 h, 2 h. After sevoflurane exposure, HUVECs were subjected to scratch assay to observe the migration distance; RT-PCR was used to detect the expression of vascular endothelial-cadherin mRNA(VE-cadherin mRNA). Statistical analysis of the data was conducted using SPSS 13.0 software package. RESULTS: Scratch assay results suggested that the migration ability of HUVECs exposed to 2% sevoflurane for 2 h were significantly inhibited during 12 h after scratch compared with the control group(P<0.01), and RT-PCR results suggested that HUVECs exposed to 2% sevoflurane for 2 h expressed greater VE-cadherin mRNA than the control group(P<0.05). CONCLUSIONS: Sevoflurane exposure for 2 h can inhibit HUVECs migration and the mechanism may be up-regulating VE-cadherin expression.

[Correlations of plasma concentrations of ß-amyloid peptide and S-100ß with postoperative cognitive dysfunction in patients undergoing oral and maxillofacial cancer surgery].

PURPOSE: To evaluate the changes of perioperative plasma concentrations of Aß1-40 and S-100ß to determine the relationship with postoperative cognitive dysfunction in elderly patients undergoing oral and maxillofacial cancer surgeries. METHODS: One hundred and fifteen patients aged at least 60 years undergoing oral and maxillofacial tumor resection were investigated between May 2014 to December 2014.Neuropsychological tests for detecting postoperative cognitive dysfunction(POCD) were performed one day before surgery and 7 days postoperatively. According to the results of neuropsychological tests on day 7, patients were divided into POCD group and non-POCD group.Plasma values of Aß1-40 and S-100ß were determined with enzyme linked immunosorbent assay (ELISA) before anesthesia induction, 24 h and 7 days after surgery. The data were analyzed using SPSS 19.0 software package. RESULTS: According to the definition, POCD was present in 37 of 115 (32.3%) patients 1 week after surgery. Compared with pre-anesthesia, S-100ß levels in POCD group were significantly increased (P<0.05); the level of Aß1-40 was significantly higher 24 h after surgery (P<0.05). Compared with non-POCD group, S-100ß levels were significantly increased 24 h postoperatively (P<0.05); Aß1-40 levels were significantly higher 24 h and 7 days postoperatively (P<0.05). CONCLUSIONS: POCD was present in 32.2% of patients on day 7 after oral and maxillofacial surgeries with general anesthesia. The increasing levels of Aß1-40, S-100ß may be associated with the occurence of POCD. Patients with long-lasting operation and high concentrations of Aß1-40 and S-100ß after surgeries were at a higher risk of POCD. The clinical values of Aß1-40 and S-100 as predictive measurements of POCD after oral and maxillofacial cancer surgery appear to be reasonable.