RESUMO
The derivatives of ganglioside GM3-NeuLacCer. NeuLacSph and NeuAcLacSphAc-were obtained and their immunomodulating properties studied. These substances are shown to inhibit lymphocyte blast-transformation independently of their ceramide structure. On the contrary, the stimulation by the above GM3-derivatives of Con A-induced T-suppressor activity depends significantly on the structure of their ceramide moiety.
Assuntos
Adjuvantes Imunológicos , Gangliosídeo G(M3)/imunologia , Gangliosídeos/imunologia , Adjuvantes Imunológicos/isolamento & purificação , Cromatografia em Camada Fina , Gangliosídeo G(M3)/análogos & derivados , Gangliosídeo G(M3)/isolamento & purificação , Humanos , Fígado/análise , Ativação Linfocitária , Linfócitos T Reguladores/imunologiaRESUMO
Specific immunosuppressive activity of cyclosporine prepared at the National Research Centre of Antibiotics by using its original producing culture was studied. It inhibited basic cellular immune responses such as DTH and transplant vs. host and prolonged the lifespan of tumor xenografts under the kidney capsule. Cyclosporine also suppressed the humoral immunity. Its inhibitory effect on lymphocyte blast transformation induced by lectins and in mixed lymphocyte cultures was shown in vitro. Cyclosporine inhibited activity of natural killer cells and T-suppressor cells induced by concanavalin A. It was demonstrated on the models of skin allotransplantation in mice and heterotopic transplantation of the heart in rats that cyclosporine prolonged the transplant lifespan. By the main indices of the cellular immunity and in the models of the transplantation immunity, cyclosporine prepared at the National Research Centre of Antibiotics was shown to be similar to that manufactured by Sandoz.
Assuntos
Células Produtoras de Anticorpos/efeitos dos fármacos , Ciclosporina/administração & dosagem , Sobrevivência de Enxerto/efeitos dos fármacos , Transplante de Coração/imunologia , Modelos Biológicos , Transplante de Pele/imunologia , Linfócitos T Reguladores/efeitos dos fármacos , Abdome , Animais , Células Produtoras de Anticorpos/imunologia , Sobrevivência de Enxerto/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Ratos , Linfócitos T Reguladores/imunologia , Transplante Heterotópico , Transplante HomólogoAssuntos
Ciclosporinas/farmacologia , Tolerância Imunológica/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Concanavalina A/farmacologia , Relação Dose-Resposta a Droga , Humanos , Imunidade Celular/efeitos dos fármacos , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/imunologia , Ativação Linfocitária/efeitos dos fármacos , Teste de Cultura Mista de Linfócitos , Fito-Hemaglutininas/farmacologiaRESUMO
Influence of chorionic gonadotropin on induction and formation of specific T-suppressors, TDTH-effectors and spontaneous killer cells, depending on its doses and schema of injections was studied. It is shown that chorionic gonadotropin has a stimulating effect on precursors of T-suppressors, not affecting the mature specific T-suppressors, causes damage to the TDTH-effectors and spontaneous killer cells, that permits chorionic gonadotropin to be recognized as an immunomodulating agent.