Application of Modern Multi-Sensor Holter in Diagnosis and Treatment.

Modern Holter devices are very trendy tools used in medicine, research, or sport. They monitor a variety of human physiological or pathophysiological signals. Nowadays, Holter devices have been developing very fast. New innovative products come to the market every day. They have become smaller, smarter, cheaper, have ultra-low power consumption, do not limit everyday life, and allow comfortable measurements of humans to be accomplished in a familiar and natural environment, without extreme fear from doctors. People can be informed about their health and 24/7 monitoring can sometimes easily detect specific diseases, which are normally passed during routine ambulance operation. However, there is a problem with the reliability, quality, and quantity of the collected data. In normal life, there may be a loss of signal recording, abnormal growth of artifacts, etc. At this point, there is a need for multiple sensors capturing single variables in parallel by different sensing methods to complement these methods and diminish the level of artifacts. We can also sense multiple different signals that are complementary and give us a coherent picture. In this article, we describe actual interesting multi-sensor principles on the grounds of our own long-year experiences and many experiments.

Pollen percentage thresholds of Abies alba based on 13-year annual records of pollen deposition in modified Tauber traps: perspectives of application to fossil situations.

Abies alba (fir), a submontane tree from Central European mountains and uplands, is of special interest for palaeoecological and palaeoclimate interpretations due to its sensitivity to air and soil humidity. Its present distribution limit in the uplands of SE Poland is still a matter of debate. In the Holocene fir expanded to Poland very late, but early fir populations are supposed to occur in the Sumava Mts (Czech Republic). The study aims: to estimate pollen thresholds for fir presence/absence in Bohemia (Czech Republic) and Poland on the basis of modified Tauber pollen traps; to use these thresholds for tracing fir presence in two pollen diagrams from Poland (Slone and Bezedna lakes) in the border zone between the Roztocze region (with fir forest stands today) and Polesie (where fir has never played an important role); and to investigate how the percentage presence/absence threshold can be used to trace the occurrence and abundance of fir trees in the Sumava Mts based on the pollen diagrams of Rokytecká slat' and Mrtvý luh. The fir pollen thresholds estimated in terms of PAR (pollen accumulation rates or pollen influx) range from 843 (grains cm-2 year-1) (Roztocze) to 61 (Krkonose) and 49 (Sumava). Percentage thresholds range from 0.3% in Krkonose where fir trees are not present within 4 km to 22% in fir-dominated woodland of the Roztocze, providing evidence of strong underrepresentation of fir in the pollen deposition. Application of these percentage thresholds to the Slone and Bezedna pollen diagrams indicates that occurrence of fir in the region is possible from 3.5 ky cal BP onwards, though the evidence is not decisive. In the Sumava, a low representation of fir pollen (1-2%) reflecting presence of scattered fir trees was detected as early as ca. 7.0 ky cal BP.

Spatial scaling of pollen-plant diversity relationship in landscapes with contrasting diversity patterns.

Mitigating the effects of global change on biodiversity requires its understanding in the past. The main proxy of plant diversity, fossil pollen record, has a complex relationship to surrounding vegetation and unknown spatial scale. We explored both using modern pollen spectra in species-rich and species-poor regions in temperate Central Europe. We also considered the biasing effects of the trees by using sites in forests and open habitats in each region. Pollen samples were collected from moss polsters at 60 sites and plant species were recorded along two 1 km-transects at each site. We found a significant positive correlation between pollen and plant richness (alpha diversity) in both complete datasets and for both subsets from open habitats. Pollen richness in forest datasets is not significantly related to floristic data due to canopy interception of pollen rather than to pollen productivity. Variances (beta diversity) of the six pollen and floristic datasets are strongly correlated. The source area of pollen richness is determined by the number of species appearing with increasing distance, which aggregates information on diversity of individual patches within the landscape mosaic and on their compositional similarity. Our results validate pollen as a reconstruction tool for plant diversity in the past.

Diabetic phenotype in mouse and humans reduces the number of microglia around ß-amyloid plaques.

BACKGROUND: Alzheimer's disease (AD) is the most common neurodegenerative disease and type 2 diabetes (T2D) plays an important role in conferring the risk for AD. Although AD and T2D share common features, the common molecular mechanisms underlying these two diseases remain elusive. METHODS: Mice with different AD- and/or tauopathy-linked genetic backgrounds (APPswe/PS1dE9, Tau P301L and APPswe/PS1dE9/Tau P301L) were fed for 6 months with standard diet or typical Western diet (TWD). After behavioral and metabolic assessments of the mice, the effects of TWD on global gene expression as well as dystrophic neurite and microglia pathology were elucidated. Consequently, mechanistic aspects related to autophagy, cell survival, phagocytic uptake as well as Trem2/Dap12 signaling pathway, were assessed in microglia upon modulation of PI3K-Akt signaling. To evaluate whether the mouse model-derived results translate to human patients, the effects of diabetic phenotype on microglial pathology were assessed in cortical biopsies of idiopathic normal pressure hydrocephalus (iNPH) patients encompassing ß-amyloid pathology. RESULTS: TWD led to obesity and diabetic phenotype in all mice regardless of the genetic background. TWD also exacerbated memory and learning impairment in APPswe/PS1dE9 and Tau P301L mice. Gene co-expression network analysis revealed impaired microglial responses to AD-related pathologies in APPswe/PS1dE9 and APPswe/PS1dE9/Tau P301L mice upon TWD, pointing specifically towards aberrant microglial functionality due to altered downstream signaling of Trem2 and PI3K-Akt. Accordingly, fewer microglia, which did not show morphological changes, and increased number of dystrophic neurites around ß-amyloid plaques were discovered in the hippocampus of TWD mice. Mechanistic studies in mouse microglia revealed that interference of PI3K-Akt signaling significantly decreased phagocytic uptake and proinflammatory response. Moreover, increased activity of Syk-kinase upon ligand-induced activation of Trem2/Dap12 signaling was detected. Finally, characterization of microglial pathology in cortical biopsies of iNPH patients revealed a significant decrease in the number of microglia per ß-amyloid plaque in obese individuals with concomitant T2D as compared to both normal weight and obese individuals without T2D. CONCLUSIONS: Collectively, these results suggest that diabetic phenotype in mice and humans mechanistically associates with abnormally reduced microglial responses to ß-amyloid pathology and further suggest that AD and T2D share overlapping pathomechanisms, likely involving altered immune function in the brain.

Wood and Its Impact on Humans and Environment Quality in Health Care Facilities.

The paper presents the application of natural materials, especially wood, which are relevant for human well-being in built environments of health, social, and day care facilities. These properties were tested by a complex methodology in a case study in the wooden waiting room at National Oncology Institute in Bratislava. In this space, experimental tests of physiological responses were further executed on 50 volunteers moving in the waiting room for 20 min. In this article, the EEG (electroencephalograph) (four persons) and emotions from the faces of all our volunteers before entering and after a stay in a wooden waiting room were recorded. Specifically, the ECG (electrocardiograph), heart rate (HR), and respiration activity were measured by using our own designed ECG holter (40 persons), and also blood pressure and cortisol levels were observed. The usage of wooden materials verifies their regenerative and positive impact on the human nervous system, through the appealing aesthetics (color, texture, and structures), high contact comfort, pleasant smell, possibility to regulate air humidity, volatile organic compound emissions (VOC-emissions), and acoustic well-being in the space.

Population and forest dynamics during the Central European Eneolithic (4500-2000 BC).

The population boom-and-bust during the European Neolithic (7000-2000 BC) has been the subject of lively discussion for the past decade. Most of the research on this topic was carried out with help of summed radiocarbon probability distributions. We aim to reconstruct population dynamics within the catchment of a medium sized lake on the basis of information on the presence of all known past human activities. We calculated a human activity model based on Monte Carlo simulations. The model showed the lowest level of human activity between 4000 and 3000 BC. For a better understanding of long-term socio-environmental dynamics, we also used the results of a pollen-based quantitative vegetation model, as well as a local macrophysical climate model. The beginning of the decline of archaeologically visible human activities corresponds with climatic changes and an increase in secondary forest taxa probably indicating more extensive land-use. In addition, important social and technological innovations, such as the introduction of the ard, wheel, animal traction and metallurgy, as well as changes in social hierarchy characterizing the same period.