Comparison of the Antioxidant Properties of Green Macroalgae from Diverse European Water Habitats by Use of Several Semi-Quantitative Assays.

Nowadays, algae are becoming more and more popular as a food group rich in nutrients, cosmetic raw materials full of antioxidants or valuable dietary supplements. They are of interest for the industry because they are found almost all over the world, in all climatic zones, both in fresh and salt waters. The aim of this study was to take a broad look at green algae (Chlorophyta) and to show how large the variability of the content of active compounds may depend on the species and the place and time of sample collection. Particular attention was paid to compounds with antioxidant activity, whose simplified profiles were created on the basis of complementary, semi-quantitative methods. Additionally, time-yield extraction optimizations were performed. Three different specimens of Ulva lactuca were compared: from the coastal zone of the Baltic Sea, from the open Baltic Sea area around Bornholm and Ulva spiralis (Ulva lactuca polymorph) from the Atlantic Ocean. The studied algae of the Cladophora genera were three different species of freshwater algae from various habitats: a lake (Cladophora glomerata), a river (Cladophora rivularis) and aquarium farming (Cladophora aegagropila, syn. Aegagropila linnaei). The content of antioxidants and the extraction efficiency varied significantly depending on the species.

Phenol-rich alternatives for Rosa x damascena Mill. Efficient phytochemical profiling using different extraction methods and colorimetric assays.

Damask rose is a well-established, abundant source of phytochemicals, as well as economically important essential oil-however, its cultivation is demanding and costly. In this paper, extracts from four raw plant materials-Salvia officinalis, Sambucus nigra, Matricaria chamomilla, Calendula officinalis, known to be rich in phenolic compounds, but also far easier to cultivate-were directly compared to those obtained from Rosa × damascena Mill. By combining diverse extraction methodologies (in a Soxhlet apparatus, ultrawave-assisted and microwave-assisted, using supercritical CO2) and complementary in vitro assays (radical scavenging, iron reducing, Folin-Ciocalteau and Al3+ complexation), it was possible to conveniently approximate and compare the phytochemical portfolios of those diverse plants. By factoring in the crop yields of different species, economically important conclusions can be reached-with pot marigold (C. officinalis) seemingly the most viable substitute for damask rose as a source of phenolics. Fatty acid and microelement analyses were also performed, to further enrich the chemical profiles of plant extracts. The paper also aims to collate and redesign multiple colorimetric assays frequently used while studying plant extracts in vitro, but criticized for their lack of correlation to in vivo activity. We show that they remain a viable tool for direct comparison of extraction methodologies, while highlighting their shortcomings.

Sweet surfactants I: Fatty acid esters of sucralose.

Surface active agents derived from the non-toxic sweetener sucralose and fatty acids of different chain length were synthesized. Obtained compounds were characterized chemically and with regard to their properties as emulsifying agents, antimicrobial preservatives and fat-soluble sweeteners. Results show that sucralose-fatty acid esters are possible multi-purpose additives, compatible with both cosmetic and edible emulsions, as well as purely oil-based, waterless formulations. Their relative effectiveness in those applications varies, and is highly dependent on the fatty acid chain length, with hydrophobic/hydrophilic character strongly impacting both emulsifying and antimicrobial properties. While the structural differences between sucrose and sucralose proved to be enough to push all of the newly synthesized compounds out of the detergent/solubilizer category of surfactants, the retention of the substrate's high sweetness is an indication that non-bitter compounds with washing capabilities are possible to obtain.

Exploring ion-ion preferences through structure-property correlations: amino acid-derived, bis(guanidinium) disiloxane salts.

In a more synthetical approach to the study of ion-specific phenomena, four dipodal bis(guanidinium) siloxanes have been synthesized starting from glycine, ß-alanine, γ-aminobutanoic acid, L-proline and 1,3-bis(3-aminopropyl)tetramethyldisiloxane. Together with their non-amide progenitor they were comparatively studied in regards to their interactions with nine different anions: sulphate, chromate, molybdate, benzoate, chloride, azide, nitrite, nitrate and thiocyanate. Their aqueous solubilities, form, 1H NMR and FT-IR spectra were examined while searching for anion-specific interactions falling in- or outside of the Hofmeister series. We show that although the "chao-" and "kosmotropic" ions affect the properties of solutions in a predictable way, more selective cation-anion pairing is responsible for phase separation and crystallinity. As a prominent example, crystal structure of one of the benzoate salts was successfully obtained and reveals a synergy of hydrophobic packing, ionic and hydrogen bonding. Immobilized but still flexible siloxane bridges give rise to crystals described by P 42/n space group and neatly segregated into hydro- and lipophilic sections.

"DIY" Silica Nanoparticles: Exploring the Scope of a Simplified Synthetic Procedure and Absorbance-Based Diameter Measurements.

In this study, the classical Stöber silica synthesis protocol was used to test the limits of simplification in the preparation and size determination of nanoparticles. The scope of three-ingredient, one-pot synthesis was established in conditions of regular 96% and 99.8% ethanol as solvent, with aqueous ammonia as the only source of base and water. Particles with diameters in the 15-400 nm range can be reliably obtained with this straightforward approach, and the direct relationship between the size and the product of concentrations of water and ammonia is evidenced. Furthermore, the idea of a linear approximation for Mie scattering in particular conditions is discussed, using experimental data and theoretical calculations. A simple, fast method for particle size determination utilizing a UV-Vis spectrophotometer-an easily accessible instrument-is explained, and shows a level of error (<0.5 SD) that can be acceptable for less rigorous laboratory use of nanoparticles or serve as a quick means for testing the influence of minor alterations to known synthetic protocols. This work aims to show that nanoparticle synthesis can (and should) become a regular occurrence, even in non-specialized labs, facilitating research into their new applications and inspiring outside-the-box solutions, while discussing the drawbacks of a more relaxed synthetic regimen.

Disiloxanes and Functionalized Silica Gels: One Route, Two Complementary Outcomes-Guanidinium and Pyridinium Ion-Exchangers.

Five novel disiloxane compounds comprising guanidinium and pyridinium moieties were obtained with high yields and purity. The verified synthetic pathways were then applied for modification of pre-functionalized silica gel, producing materials with the analogous organic side-chains. These halide-containing compounds and materials were then compared as to their ion-exchange properties: two disiloxanes proved to be effective in leaching different anions (nitrate, benzoate and ascorbate) from solid to organic phase, and pyridinium-functionalized silica gels showed selectivity towards perchlorate ion, removing it from methanolic solutions with preference to other singly charged anions. The results presented demonstrate that both compounds and materials containing silicon-carbon bonds can be produced using the same methodology, but offer strikingly different application opportunities. Comparison of their properties provides additional insight into the binding mode of different anions and hints at how the transition from a flexible siloxane bridge to immobilization on solid surface influences anion-binding selectivity. Additionally, one of the siloxane dipodands was found to form a crystalline and poorly soluble nitrate salt (1.316 g/L, water), although it was miscible with a wide range of solvents as a hydrochloride. A possible explanation is given with the help of semi-empirical calculations. A simple, time- and cost-efficient automated potentiometric titration methodology was used as a viable analytical tool for studying ion-exchange processes for both compounds and materials, in addition to standard NMR, FT-IR and ESI-MS methods.