RESUMO
BACKGROUND: Toxoplasmosis led to the death of two Brachyteles arachnoides, an endangered atelid. METHODS: The diagnosis was established by necropsy, histopathological, immunohistochemical and ultrastructural changes. RESULTS: The analysis confirms the presence of Toxoplasma gondii. CONCLUSIONS: This report contributes to the development of protocols for health surveillance on maintenance and conservation of southern muriquis.
Assuntos
Animais de Zoológico/parasitologia , Atelinae/parasitologia , Doenças dos Macacos/diagnóstico , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/diagnóstico , Animais , Brasil , Conservação dos Recursos Naturais , Espécies em Perigo de Extinção , Evolução Fatal , Masculino , Doenças dos Macacos/parasitologia , Toxoplasmose Animal/parasitologiaRESUMO
BACKGROUND: Few authors have been attempting between mast cells and dermal dendrocytes interactions on urticaria. OBJECTIVE: To describe the extruded mast cell granules and dermal dendrocytes in drug-induced acute urticaria. METHODS: Seven patients with drug-induced acute urticaria were enrolled in the study. We token skin biopsies of urticaria lesion and perilesional skin. The 14 fragments collected were processed to immunogold electron microscopy using single stains to tryptase and FXIIIa, besides double immunogold labeling with both. RESULTS: Some sections demonstrated mast cells in degranulation process, both in anaphylactic and piecemeal degranulation types. After double immunogold staining, 10 nm (FXIIIa) and 15 nm (Tryptase) gold particles were present together over the granules in mast cells indicating that tryptase and FXIIIa are each localized within the granules of these cells. Interestingly, we found a strong evidence of than the exocytosed mast cell granules contents both FXIIIa and tryptase immunolabeled are phagocytized by dermal dendrocytes. CONCLUSIONS: The current observations provide morphological evidence that the exocytosis-phagocytosis mechanisms of mast cell granules represents one pathophysiological example of mast cells-dermal dendrocytes interactions in urticaria.
Assuntos
Comunicação Celular , Fagocitose/fisiologia , Urticária/induzido quimicamente , Urticária/patologia , Adulto , Grânulos Citoplasmáticos/patologia , Derme/citologia , Derme/patologia , Fator XIIIa/metabolismo , Feminino , Humanos , Imuno-Histoquímica , Mastócitos/citologia , Mastócitos/patologia , Microscopia Eletrônica de Transmissão/métodos , Microscopia Imunoeletrônica/métodos , Pessoa de Meia-Idade , Estudos de AmostragemRESUMO
BACKGROUND: An adult male Brachyteles arachanoides, kept in captivity since 1990, was found dead without apparent clinical evidence. METHODS: Necropsy report, histopathology, immunohistochemistry, and ultrastructural examination were conducted. RESULTS: Pulmonary syncytial cells were positive for respiratory syncytial virus (RSV), and ultrastructural examination revealed viral particles inside macrophages compatible with the Paramyxoviridae family. CONCLUSIONS: Muriquis are susceptible to RSV pneumonia followed by respiratory distress syndrome and death.
Assuntos
Atelinae/virologia , Doenças dos Macacos/virologia , Pneumonia Viral/veterinária , Infecções por Vírus Respiratório Sincicial/veterinária , Animais , Evolução Fatal , Imuno-Histoquímica/veterinária , Pulmão/patologia , Pulmão/virologia , Macrófagos/virologia , Masculino , Doenças dos Macacos/patologia , Pneumonia Viral/patologia , Pneumonia Viral/virologia , Infecções por Vírus Respiratório Sincicial/patologia , Infecções por Vírus Respiratório Sincicial/virologia , Vírus Sinciciais Respiratórios/isolamento & purificação , Vírion/isolamento & purificaçãoRESUMO
This study reports the occurrence of renal lesions in cetaceans from the coast of Brazil subjected to necropsy examination between 1996 and 2011. The animals (n = 192) were by-caught in fishing nets, were found dead on beaches or died despite attempted rehabilitation. Kidney samples were evaluated grossly and microscopically and, depending on the histopathological findings, immunohistochemical and ultrastructural analyses were conducted. Due to autolysis, a diagnosis was reached in only 128 animals, of which 82 (64.1%) had kidney lesions. Cystic renal disease was the most common lesion observed in 34 cases (26.6%) and these were classified as simple cysts in eight cases (6.3%), polycystic kidney disease in one rough-toothed dolphin (Steno bredanensis), secondary glomerulocystic disease in 16 cases (12.5%) and primary glomerulocystic disease in nine cases (7%). Other lesions included membranous glomerulonephritis (28 cases; 21.9%), membranoproliferative glomerulonephritis (20 cases; 15.6%), lymphoplasmacytic interstitial nephritis (21 cases; 16.4%), lipidosis (19 cases; 14.8%), glomerulosclerosis (8 cases; 6.3%) and pyogranulomatous nephritis(five cases; 3.9%); two of the later were associated with the migration of nematode larvae. Additionally, tubular adenoma was identified in a Franciscana (Pontoporia blainvillei). The pathological implications of these lesions are discussed according the cause of death, age or sex of the animals. Furthermore, the lesions were compared with those of other marine and terrestrial mammals, including man.
Assuntos
Cetáceos , Nefropatias/veterinária , Rim/patologia , Animais , Brasil , Incidência , Nefropatias/epidemiologia , Nefropatias/patologiaRESUMO
Lung fragments from 12 patients were collected immediately after death and studied by light and electron microscopy and by immunohistochemistry to describe the main morphologic and ultrastructural aspects of the lung and platelets in leptospirosis (Weil's syndrome), to search for the possibility of disseminated intravascular coagulation (DIC), and to assess the relationship between endothelial lesions and local platelet aggregation and the leptospiral antigen distribution, as well as its relationship with the intensity of the lesions. The immunohistochemical results for fibrin aggregates were positive in the lumen and/or on the vascular endothelium in nine cases and on the alveolar surface in seven cases, leading to the diagnosis of the adult respiratory distress syndrome in these seven cases. Test results for leptospiral antigen by immunohistochemistry were positive in eight cases with no direct relationship between antigen deposits in the pulmonary vascular endothelium and intensity of the lesions. The ultrastructural findings were uniform and constant. Capillary lesions were characterized by swelling of endothelial cells, an increase in pinocytotic vesicles, and giant dense bodies in the cytoplasm of these cells. No necrosis, rupture, nor exposed subendothelial collagen was observed outside the hemorrhagic areas, and the intercellular junctions were preserved. The lung involvement in severe human leptospirosis presents as hemorrhagic pneumopathy with septal capillary lesions that are the usual cause of death. The thrombocytopenia that was verified in 11 of 12 patients in our study seems to bear no relationship to DIC and seems to be determined by activation, adhesion, and aggregation of platelets to the stimulated vascular endothelium, with an amorphous electron-dense substance between the endothelial cells and the adherent platelets in places where the subendothelial collagen was not exposed.
Assuntos
Pulmão/patologia , Trombocitopenia/etiologia , Doença de Weil/patologia , Adulto , Antígenos de Bactérias/análise , Plaquetas/imunologia , Plaquetas/metabolismo , Plaquetas/ultraestrutura , Capilares/parasitologia , Capilares/ultraestrutura , Adesão Celular , Endotélio Vascular/metabolismo , Endotélio Vascular/parasitologia , Endotélio Vascular/ultraestrutura , Feminino , Fibrina/análise , Fibrina/imunologia , Hemorragia/etiologia , Humanos , Imuno-Histoquímica , Leptospira interrogans/imunologia , Leptospira interrogans/isolamento & purificação , Pulmão/irrigação sanguínea , Pulmão/ultraestrutura , Pneumopatias/etiologia , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Agregação Plaquetária , Trombocitopenia/patologia , Doença de Weil/complicaçõesRESUMO
This study describes toxoplasmosis in a by caught Guiana dolphin (Sotalia guinensis) from Paranaguá Bay, Paraná, Brazil. Interstitial pneumonia, multisystemic arteritis, multifocal adrenalitis and hepatitis were the primary lesions observed. These tissues had moderate to severe necrosis and mononuclear cells infiltration usually surrounded by tachyzoites and tissue cysts. Moderate lymphoid depletion was evident in the spleen. Toxoplasma gondii was positive by immunohistochemical and ultrastructural evaluation. Furthermore, the animal was negative for Morbillivirus by immunohistochemistry and had low levels of persistent organochlorines. There is evidence of environmental changes in the Paranaguá Bay that could justify the occurrence of toxoplasmosis in Guiana dolphin. The sewage run-off from main urban areas and the presence of domestic and wild felids in areas surrounding the bay could be a source of T. gondii oocysts from land to sea. Based on its habitat, the authors recommend this dolphin species as sentinels for the health of bays and estuaries where they occur.
Assuntos
Golfinhos/parasitologia , Toxoplasmose Animal/patologia , Animais , Brasil , Feminino , Toxoplasma/fisiologiaRESUMO
A fast method for processing biologic material for electron microscopy for precise and specific diagnosis of infectious agents is an increasing necessity. After different, reportedly fast methods were tested, a useful and quick technique was developed that provides well-preserved cellular structures, enabling the etiologic diagnosis of infectious agents even in necrotic tissue or other biologic material such as sputum, bronchoalveolar lavage, and the like. This procedure takes less than 3 hours.
Assuntos
Técnicas de Preparação Histocitológica , Infecções/diagnóstico , Microscopia Eletrônica , Síndrome da Imunodeficiência Adquirida/complicações , Líquido da Lavagem Broncoalveolar/microbiologia , Líquido da Lavagem Broncoalveolar/parasitologia , Humanos , Intestino Grosso/microbiologia , Intestino Grosso/parasitologia , Intestino Grosso/ultraestrutura , Fígado/microbiologia , Fígado/parasitologia , Fígado/ultraestrutura , Pulmão/microbiologia , Pulmão/parasitologia , Pulmão/ultraestrutura , Linfonodos/microbiologia , Linfonodos/parasitologia , Linfonodos/ultraestrutura , Pneumonia por Pneumocystis/complicações , Pneumonia por Pneumocystis/diagnóstico , Escarro/microbiologia , Escarro/parasitologia , Toxoplasmose/complicações , Toxoplasmose/diagnósticoRESUMO
The fat cells of the fascia areolaris and fascia lamellaris of men, women, and pregnant women (aged between 20 and 35a) were morphometrically studied. The cell volumes showed the following average values: 4.423 X 10(5) micron3 and 2.004 X 10(5) micron3 for the fasciae areolaris and lamellaris respectively, in men; 6.236 X 10(5) micron3 and 3.964 X 10(5) micron3 in women, and 10.114 X 10(5) micron3 and 4.635 X 10(5) micron3 in the pregnant women. The analysis of variance showed significant differences between both sexes, and fasciae areolaris and lamellaris. The differences between women and pregnant women as far as the cell volume is concerned, in both fasciae, were not significant. As to the fascia areolaris, not the lamellaris, the difference between the sexes was significant.
Assuntos
Tecido Adiposo/citologia , Fáscia/citologia , Adulto , Análise de Variância , Técnicas Citológicas , Feminino , Virilha , Humanos , Masculino , Gravidez , Caracteres SexuaisRESUMO
In order to verify the effects of CO2 laser on the palatine mucosa of rats, 15 animals were utilized. The animals were anaesthetized with thionembutal by intra-peritoneal injection and the palatine mucosa of each animal was irradiated with a single pulse of 10 W. The results showed that immediately after the irradiation of CO2 laser, the formation of small cavities is observed. The wound healing post operationem is gradually in 1 week. The region is totally occupied by neoformed connective tissue and epithelial cells. Numerous polymorphonuclear cells and fibroblasts may be seen between the neoformed collagen fibres.
Assuntos
Lasers , Palato/efeitos da radiação , Animais , Masculino , Microscopia Eletrônica de Varredura , Mucosa/efeitos da radiação , Mucosa/ultraestrutura , Palato/ultraestrutura , Ratos , Ratos EndogâmicosRESUMO
The fat cells of the so called fascia areolaris and fascia lamelaris (Velpeau 1834; Sterzi 1910) of men and women (aged from 20 to 35 years) were ultrastructural and morphometrically (cell volume) studied. No noteworthy submicroscopic difference was observed between fascias. The cell volumes obtained from planimetric measures showed the following values: 3.770 X 10(5) microns and 2.497 X 10(5) microns in the fascia aerolaris and lamelaris of men, respectively. For the women the values were: 7.222 X 10(5) microns and 5.025 X 10(5) microns (Fig. 3). The analysis of variance shows significant differences between the sexes and between fascia areolaris and lamelaris. The difference between the fascias supports the Sterzi's (1910) description on the tela subcutanea as being formed by those two distinct layers.