RESUMO
For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.
Assuntos
Proteínas , Síncrotrons , Cristalografia por Raios XRESUMO
An expert assessment of the fitness for work and vigour of women after gynaecological cancer operations has to be made by an experienced medical specialist who is well acquainted with the problem. The role of individual decisions on the basis of secure scientific knowledge gets more and more dominating with emphasis placed on the assessment of functional achievements. -Important assessment criteria are the following: stage of tumour, histology, degree of differentiation, type of therapy and possible consequences of therapy as well as psycho-social factors and elements of working medicine. In any case the consideration of individual consequences of illness and medical treatment remains the decisive criterion at the time of evaluation. -This paper should serve as guidance for the assessment of disablement/disability of women after gynaecological cancer operations in the framework of a graduated therapy concept.