RESUMO
We looked for differentially expressed genes at different stages of preadipocyte differentiation and examined their functions, based on DNA microarrays of preadipocytes obtained from healthy subjects undergoing cosmetic liposuction. We downloaded gene expression profile GSE25910 from the Gene Expression Omnibus database and identified the differentially expressed genes with packages in R language. The selected differentially expressed genes were further analyzed using bioinformatics methods. Comparing gene expression at different stages of preadipocytes differentiation, we found that expression of 668 and 1007 genes were altered in middle and late stages compared with the early stage, respectively. Function analysis revealed that the differentially expressed genes were mainly related to fatty acid metabolic processes in the former two stages.
Assuntos
Adipogenia/genética , Ácidos Graxos/metabolismo , Obesidade/genética , Adipócitos/citologia , Adipócitos/metabolismo , Células-Tronco Adultas/citologia , Células-Tronco Adultas/metabolismo , Ácidos Graxos/genética , Perfilação da Expressão Gênica , Humanos , Análise de Sequência com Séries de OligonucleotídeosRESUMO
Atrial natriuretic peptide (ANP) present in the brain has been reported to have profound effects on water and salt metabolism. This study was designed to observe the effect of intracerebroventricular (ICV) injection of ANP on drinking behavior of rats, induced by centrally administered angiotensin II (Ang II) and 24-hours water deprivation, by using a T-maze to measure the speed they ran in a runway for water rewards. In 24-hours water deprived rats ICV injection of ANP resulted in a significant decrease of either running speed or water intake. Drinking behavior induced by ICV injection of Ang II in normally hydrated rats was also significantly inhibited by a prior injection of ANP. These findings suggest that ANP in the brain plays an important role in the central control of drinking behavior.
Assuntos
Angiotensina II/farmacologia , Fator Natriurético Atrial/farmacologia , Comportamento de Ingestão de Líquido/efeitos dos fármacos , Animais , Injeções Intraventriculares , Masculino , Ratos , Ratos Endogâmicos , Privação de ÁguaRESUMO
It has been demonstrated that atrial natriuretic polypeptide (ANP), or atriopeptin, is synthesized and stored not only in the cardiac atrial myocyte but also in the central nervous system, especially in the hypothalamus. ANP may play an important role in the regulation of fluid and electrolyte balance and blood pressure in the peripheral and central nervous systems. Some of the biological actions of ANP are opposite to those of angiotensin II and arginine vasopressin in the central nervous system. However, the mechanism of action of ANP remains, in a large part, to be defined.